A) DNA polymerase binds to a single-stranded DNA template (blue) and synthesizes a complementary strand of DNA (red). B) When DNA polymerase randomly incorporates a fluorescently labeled ddNTP ...

Shichino said, “ddNTP spike-in, specifically dideoxycytidine phosphate (ddCTP), stops the excessive extension of polyN-tail by TdT in a stochastic manner, ...

TAS-Seq employs a nanowell/magnetic bead-based single-cell RNA sequencing platform, terminal transferase enzyme, and dideoxynucleotide phosphate terminators to generate high-precision information ...

When a ddNTP is incorporated into a chain of nucleotides, synthesis terminates. This is because the ddNTP molecule lacks a 3' hydroxyl group, which is required to form a link with the next ...