Desmond Miles continues to travel the memories of Ezio Auditore, bringing him to turn-of-the-century Rome with Ezio rebuilding the assassin order to bring down the tyrannical Borgia rule.
Desmond Miles is trained to become a modern-day Assassin through the resurrected memories of ancestor Ezio Auditore da Firenze, who uncovers a massive Templar conspiracy in Renaissance Italy leading to the new Pope.