Saddest Pixar moments?

After catching an action figure ad on TV that supports Woody's claim, Buzz Lightyear learns the hard way that he is a toy.

Andy spurs Woody in favor of his new toy, Buzz Lightyear.

Flik is banished from the colony.

When She Loved Me (Jessie's Story)

Bye to Boo

Losing Coral (and All of Nemo's Unhatched Siblings)

Mr. Incredible thinks his family is dead.

Doc's Story

the inability of the viewer to taste the onscreen food

EVE manages to repair and reactivate a severely damaged WALL-E, but he does not remember her.

Married Life (the end of the prologue, a.k.a. the first 20 minutes of the movie)

Facing imminent doom at the landfill incinerator, the toys accept their fate and hold one another's hands.

Andy gives away and says goodbye to his toys.

The general existence of this movie

something about her mom

Mike feels left out or something

Bye Bye Bing Bong

Riley cries at her new school.

Riley returns to her family and so do her core memories.