Oranges and the orange tree are used for testing the ability to synthesize objects from the digital world. In Tron (1982) the very first item digitized was an orange.
The real-world motorcycles used in this movie are Ducati, which was also the same make as the bike ridden by Sam Flynn in Tron: El legado (2010).
On April 29, 2024 it was announced by Disney that Jeff Bridges would return, making him the only actor to appear in all three films.
In one of the archival video clips of Eve that Ares studies, she's wearing a Daft Punk shirt. Daft Punk composed the music for the previous Tron film, Tron: El legado (2010).
Jared Leto spent the entire shoot in-character as Ares, not speaking to anyone unless necessary and having everyone refer to him as such. Jeff Bridges was the only one who didn't follow this rule, reasoning that since his character, Kevin Flynn, is the creator of the Grid, he's therefore above Ares and could call him whatever he wanted.