During the montage where Big Jack Horner assembles his Baker's Dozen, the horses pulling their carriage were actually unicorns whose horns were cut off
After Death cuts Puss above the eye in the beginning, he has a scar there for the rest of the movie.
The film employs a stylized animation similar to Spider-Man: New Generation (2018) and Les Bad Guys (2022). The filmmakers decided to move away from the realistic animation style employed in Shrek (2001) and Le Chat potté (2011) in favor of a more creative, larger-than-life style to showcase Puss in Boots' adventure.
During the fight with the giant, Puss is flung into a bakery store sign. Freezing the image before he hits it shows it's a Jack Horner bakery, foreshadowing his presence later in the film.
The filmmakers cite the Western Le Bon, la Brute et le Truand (1966) as an influence on the film: "They're all after the ultimate fairytale prize. Everybody feels they have to have the wish, which really just leads to a great kind of action adventure with great momentum." In one scene, the protagonists and the antagonists are also involved in Mexican Standoff and their faces are shown with extreme close-ups as a nod to Sergio Leone's movie.