In Jack's first pre-wedding flashback, he does not sport his distinctive shoulder tattoo - something that is explained later in the series. Indeed, in the Blu-ray presentation, it can be clearly seen that Matthew Fox's real tattoo has been painted over.
The title is a portion of the Hippocratic Oath doctors take, vowing to first and foremost, do no harm.
At the end of the episode, we see many of the "other survivors" that have never been centered on. They still have no dialogue but many, both men and women, can be seen clearly, more than other episodes.
Sawyer calls Jin "Kato." This is a character from TV series The Green Hornet (1966-1967). Kato was played by Hong Kong-American actor, Bruce Lee.
Julie Bowen, who plays Matthew Fox's wife in this episode, appeared on an episode of Matthew's first series, Party Of Five.