- His parents are Egyptian. He has also said that he has "an eighth Greek" ancestry.
- He had the Freddie Mercury teeth he wore while filming Bohemian Rhapsody (2018) cast in gold.
- His identical twin brother Sami, who is younger by four minutes, is a teacher.
- His late father was a tour guide in Cairo and later sold insurance. His mother is an accountant.
- Attended high school with Kirsten Dunst, where he was a year ahead. They had a musical theater class together.
- His older sister Yasmine is an emergency medical doctor.
- Received his Bachelor of Fine Arts degree from the University of Evansville in Evansville, Indiana. (2003)
- Attended high school with and once dated his Mr. Robot (2015) co-star Christian Slater's stepsister Emily.
- Graduated from Notre Dame High School in Sherman Oaks, Los Angeles (June 1999). Two of his classmates were Rachel Bilson and Taylor Fry. Kirsten Dunst graduated from the same school a year later, and Katharine McPhee graduated three years after Rami.
- Won the 2019 Academy Award in the Best Actor in a leading Role category for his role as Freddie Mercury in Bohemian Rhapsody (2018).
- Raised in the Coptic Orthodox faith.
- He is the fifth Oscar winning actor to play a main villain in a James Bond film after Christopher Walken, Benicio Del Toro, Javier Bardem and Christoph Waltz.
- Won the 2019 Golden Globe Award in the Best Performance by an Actor in a Motion Picture - Drama category for his role as Freddie Mercury in Bohemian Rhapsody (2018).
- Almost reprised his role as Freddie Mercury in a cameo appearance in Rocketman (2019), before the producers ultimately decided against this.
- He is the second actor with ancestry from an Arab country to win the Academy Award for Best Actor. F. Murray Abraham, who won for his role in Amadeus (1984), is the first actor.
- Attended the worldwide Women's March protest, while he was in Paris, France.
- Raised in Los Angeles, California.
- Was in a relationship with his Bohemian Rhapsody (2018) co-star Lucy Boynton from 2018 until 2023.
- Rami's Best Actor Oscar for Bohemian Rhapsody (2018) was presented to him by Gary Oldman and Allison Janney (Dolby Theatre, Los Angeles). (24 febbraio 2019)
- Is good friends with Bassam Habib.
- The first American actor to play a main James Bond villain since Robert Davi in 007 - Vendetta privata (1989).
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