The mad scientist who never speaks is named Fritz Huhnmörder. "Huhnmörder" is German for "chicken murderer".
In the credits of every episode, Sarah Michelle Gellar and Mila Kunis have a special spoof credit, and in most seasons, there is a different person from the staff given a spoof credit right after them.
Over sixty titles were considered for this show, including "Junk in the Trunk", "ADD TV", "Gold Dust Gasoline", "Toyz in the Hood", "Vegetable Fun Fest", and "S&M Presents", but all were rejected due to trademark or other legal reasons. Out of the many titles the show's creators submitted, only "Robot Chicken", the name of a local Chinese restaurant dish, was accepted. Some of the rejected names were later used as episode titles.
Les Claypool is the composer of the theme song. He also composed and sang the theme to South Park (1997), and is the leader of the band, Primus.
The closing theme music is a chicken-clucking version of The Gonk, a piece of music played in the mall in Zombi (1978) that was also used for the closing credits of the movie.