In the first few episodes, the production uses colored light to great effect to separate out each individual Defender story. Each scene involving Daredevil has a hint of red. Jessica Jones is lit with purple. Iron Fist with green and Luke Cage is lit in shades of yellow. As the series progresses and the Defenders become aligned, the four colors eventually turn into more neutral whites and blues.
In this series, the founding members of the Defenders are Daredevil, Jessica Jones, Luke Cage and Iron Fist. In the comics, the Defenders were formed by The Incredible Hulk, Doctor Strange, Silver Surfer, and Namor the Sub-Mariner.
When Matt and Jessica are leaving the police station, a photo of Stan Lee can be seen on a wall in the background.
The IP address hidden in the first teaser trailer leads to a site with information about The Defenders.
The budding friendship between Luke Cage and Danny Rand is a reference to the Heroes for Hire, a small business started by the two in the comics. Both actors have stated their desire to make a "Heroes for Hire" series.