This film contains a "Rogue's Gallery" of villains for Wonder Woman including Dr. Poison, Dr. Cyber, Cheetah, Giganta, Veronica Cale, Medusa, and Silver Swan.
First on-screen portrayal of the "purple healing ray" used by the Amazons of Themyscira.
Long time comic fans will recognize "Ferdinand the Minotaur", Diana's long-time friend in the comics, as the minotaur who protects the maze.
Rosario Dawson was in the previous solo animated Wonder Woman movie, Wonder Woman (2009) however, she was the voice of the Amazon named "Artemis" in the 2009 film rather than the titular Wonder Woman in Bloodlines.
This film takes inspiration from the Rebirth (2016) comic run of Wonder Woman. Most notably, the character of Etta Candy is African American as she is in Rebirth unlike most other comic portrayals where she is Caucasian.