One of the band members is Olive Oyl's brother Castor, in a nonspeaking role. He is the short man who plays the flute.
Another of the band members is Oscar, Popeye's friend and Swee' Pea's bucktooth babysitter from the Thimble Theatre comic strip.
The unkempt wig worn by Bluto is a reference to the hair style of famed conductor Leopold Stokowski.
Popeye eats his can of spinach in three separate gulps instead of his usual "chugging" of the entire can at once.
Fantasia (1940) was still 5 years away when this classical-music-based short was released. Of course, it was much more slapstick, as the series generally was.