According to Gerry Anderson, the plot of Joe 90 was based around Gerry's pre-Supermarionation days when he served as an assistant editor for such films as The Wicked Lady (1945), handling recording tape on a daily basis. While pondering on the blanking and re-use of such tape, Anderson made a connection to the human brain's electrical activities, as he would later explain, "I read somewhere that the human brain is controlled by electrical impulses and how thoughts are stored electronically. I started toying with the story potential of a process that would allow the recording of brain patterns and transferring them to another brain. I was really likening it to magnetic recording, where material could be stored or transferred to another tape."
In most of the Gerry Anderson shows including Joe 90, all vehicles drive on the right even though Joe 90 is set in England where they drive on the left. Gerry Anderson would later explain in an interview for UFO (1970) which was set in then near future of 1980, that he thought the United Kingdom would convert over to driving on the right side of the road in the future.
Most of the guest puppets were previously used in Captain Scarlet and the Mysterons (1967) and would later be used again in The Secret Service (1969).
The device used to transfer the brain patterns of various experts in their fields, brain surgeons, fighter pilots etc in to Joe was called the B.I.G. R.A.T. which stood for Brain Impulse Galvanoscope Record And Transfer.
Joe is left-handed, as seen from his using his pistol in his left hand.