At 37:46, Mary asks the meaning of "La tetilla del diablo." Ambrose lies and says it means "Welcome home wayward pilgrims." In truth it means "the devil's nipple."
Michael Parks, who plays Ambrose Bierce, also appeared in the original From Dusk Till Dawn (1996), playing a completely different character. His real life son, James Parks, had a small role in From Dusk Till Dawn 2: Texas Blood Money (1999).
At 58 minutes, Razor sings "La Cucaracha" but substitutes his own lyrics "marijuana para fumar" (marijuana to smoke).
The subtitle of the film, "The Hangman's Daughter," is taken from the title of a short story co-authored by Ambrose Bierce (who appears as a character in the film) - "The Monk and the Hangman's Daughter," Ambrose Bierce and Adolphe Danziger De Castro, 1911.
George Clooney, who played Seth Gecko in the original From Dusk Till Dawn (1996), said the inspiration for his character's tattoo was taken from recently watching Once Were Warriors (1994). The Hangman is played by Temuera Morrison who was one of the main actors in Once Were Warriors (1994).