In a multiplayer game, tapping the d-pad causes your character to say praises to the gamer whose turn it is. Tapping the c-buttons causes them to insult the player.
Most of the characters have a unique ball animation if they achieve a "nice shot." For instance, Mario's ball turns into a fireball, and Yoshi's ball leaves a rainbow trail.
The non-Mario characters Plum, Sonny, Harry, Maple and Charlie, that are introduced in the game, are new characters created by Camelot Software Planning specifically for the game.
Metal Mario is an exclusive unlockable character in the western version of Mario Golf. In the Japanese version, he is absent from the game. Metal Mario is unlockable by gaining a Birdie Badge on every course.
Princess Peach has a shorter skirt in this game than in other games. However, in one of her "course complete" animations, it "grows" back to its normal size.