In the film, Moe is in charge of the seed money. In reality, Moe Howard kept track of all of The Three Stooges' finances--Larry Fine liked to play the horses and Curly Howard would spend much of his money on women. So Moe made sure that some of their money was invested for when their careers ended. Moe himself retired a wealthy man.
In the 1970s, Mel Brooks sought to write and direct a Three Stooges movie starring himself, Marty Feldman and Dom DeLuise as Moe, Larry and Curly. The trio eventually made Silent Movie (1976) instead.
Caroline Kimberly Scott, who plays one of the nuns in the beginning of the movie, is the great-granddaughter of Moe Howard, one of the original The Three Stooges.
Voice actor Billy West -- who had based the voice of Stimpy from The Ren & Stimpy Show (1991) on Stooge Larry Fine -- worked on-set as a dialect coach for Sean Hayes, who was playing Larry.
The orphanage is shown to be founded in 1934, the same year The Three Stooges began their series of shorts for Columbia Pictures, which continued until 1958.