Balto, Jenna, Boris, Muk, and Luk are the only characters to appear in all three Balto films.
During the E.D.A.S. sequence, Kaltag (now with a darker reddish-gold colored coat, dark brown ears instead of his black-tipped ears and without his brown left eye patch and collar) and Nikki (now with a lighter back patch replacing his back stripes and without his collar) can be seen in two open windows of the hotel, and a Balto look-alike can be seen on the roof of John's Bar next door. Additionally, the terrier from Balto: Wolf Quest (2001) can be seen. Furthermore, the dog with the twitching eye, and the Saint Bernard-like dog from Balto (1995) can also be seen.
In an opening scene, Rosy, Balto, and Jenna can briefly be seen huddled around a small fire.
After this movie's release, 'Kodi' became one of the most popular sled dog names.
"You think your job is worth more than a life?" is said twice throughout the movie, once by Jenna and once by Balto.