The voice of Bowser Koopa was actually provided by a woman, Akiko Wada, a famous singer from Japan.
This is the 1st time Luigi appears taller and thinner than Mario.
The film seems to have inspired certain elements of the Mario games that came afterwards, such as Super Mario Bros. 3's giant enemies and flying ship, Super Mario World allowing Mario to hijack Lakitu's cloud, and Mario grabbing Bowser by the tail and spinning him around would become iconic in Super Mario 64,
The name of the song played during the end credits is "I Love You", sung by Mami Yamase who also voiced Peach in this movie.
The first ever animated adaptation of the "Super Mario Bros." video game, not counting Mario's appearance in the show "Saturday Supercade".