Madame Hydra appears in this game as a member of HYDRA during World War II. In Agents of S.H.I.E.L.D. (2013), she is a member of HYDRA existing within the Framework.
The Sleeper appears in this game as a large robot used by HYDRA as a superweapon. In Agents of S.H.I.E.L.D. (2013), Sleeper Mechs are robot soldiers under the command of General Hale.
Wolfgang von Strucker appears in this game as a high-ranking member of HYDRA during World War II. In Captain America: The Winter Soldier (2014) and Avengers: Age of Ultron (2015), he is one of the leaders of HYDRA in the 21st Century and a former sleeper agent within S.H.I.E.L.D.. The version in the game has also had the Satan Claw irreversibly grafted to his arm, while the canon version has no Satan Claw.