At Mayu's house, her mother Sumire prepares for the opening of her store, Pretty Holic. Meanwhile, Satoru is continuing his research on the Garugaru, believing that gathering more information will help the Cures locate them.
While walking Komugi, Iroha meets a girl named Ema, her mother, and their Pomeranian, Pon, and offers to take them to Friendly Animal Hospital and Salon.
Following the Kirarin Penguin's return to Niko Garden, Komugi once more expresses her frustration at being unable to use her Tact's powers, with Mey Mey stating that this may be because she lacks something important as a Pretty Cure.
At the dog run, Komugi encounters Satoru, who takes her to his house. While Iroha and her parents are looking for Komugi, he calls them to tell them that she is at his house, but advises them to wait some time, as she is still upset.
At Pretty Holic, which has received more customers, Sumire tells Mayu that she can design a new product to sell. Throughout the day, Mayu struggles to come up with new ideas for the product, despite her classmates' suggestions.
After Mayu tells Iroha that Yuki sometimes disappears, Iroha decides to help her on her day off from school. At Mayu's house, Komugi and Iroha set up a camera to monitor Yuki and go outside to have Komugi track her scent.
After Mayu notices that Yuki has been refusing to eat and is not in her usual spots, she takes her to Friendly Animal Hospital and Salon, where Youko determines that she will be fine with rest.
On their way to school, Komugi and Iroha run into Youko, who is on her way to work at the zoo and finds Komugi familiar, even though she has never met her as a human.
Since it is baby animal season in Animal Town, Komugi, Iroha, Mayu, and Satoru go on a baby animal tour and see birds, deer, rabbits, kittens, and ducklings.
As Komugi, Iroha, and Satoru search for the hamster Garugaru, which fled following their first battle with it, the mysterious voice powers it up by commanding it to take revenge on Nyammy for harming it.
With Mayu having become Cure Lillian, the group heads to Niko Garden to introduce her and Yuki to Mey Mey. When he asks them to help save the Niko and Kirarin Animals.
Yuki transfers to Second Wangan Junior High under the guise of being Mayu's cousin and soon becomes popular among the students for her skills, but she refuses to work with Komugi and Iroha and refuses to participate in school activities.
Komugi and Iroha's friend Inuzuka, who works as a dog trainer, invites them to try dog agility by navigating an obstacle course, with Mayu, Yuki, Satoru, and Daifuku watching.
The Cures and Satoru visit their classmate Okuma's house, a farm run by her family. There, they visit the animals and try out farming as part of a stamp rally to win souvenirs.
In the morning, Komugi is awoken by Niko's egg shining and discovers that it has cracked. Along with the Cures, Satoru, and Mey Mey, they go to the dog run, where the egg hatches and Niko emerges from it.
At the Mirror Stone, Niko reveals to Mey Mey that it is a piece of the Niko Diamond that she gave to the animals to grant their wish to be friends with humans.
After remembering how she was afraid of an elephant when she visited the zoo with her parents as a child, Iroha decides that they should go to the zoo since she has not taken Komugi there, with Niko and Satoru going with them.
At Friendly Animal Hospital and Salon, Iroha prepares for her first date with Satoru. Although Tsuyoshi is reluctant for them to go out, he accepts it after realizing that they have a common interest in animals.
On Halloween, the residents of Animal Town have dressed up to go trick-or-treating, including the Cures, Satoru and Daifuku, and their friends and classmates.
The Cures, along with Satoru, Niko, and Mey Mey, go to Toboe Shrine, which was built to honor the extinct wolves, in search of Gaou. Although they can sense his presence and that he has been there recently, he is not there.
Mayor Washio asks Takayuki to photograph people's pets for the yearly animal calendar, and the Cures and Satoru decide to show him around Animal Town so that he can take pictures.
At school, Mayu's classmates ask her to make them scarves after seeing that she and Yuki have matching scarves, but she instead decides to teach them how to knit since they do not know how.
While going out, Komugi and Iroha encounter Otsuru, who is taking her dog - Fuku - to Friendly Animal Hospital and Salon for a check-up after their walk.
While the Cures fight the Gaogaons, Niko confronts Gaou directly and attempts to reason with him, saying that, despite his desire for revenge on humans, the humans and animals of Animal Town are not the same as the ones he knew.
In a flashback, Subaru, along with Torame and Zakuro, head to the summit of Mt. Toboe, where he informs Gaou of the villagers' decision to send hunters to kill the wolves.
Subaru and Gaou reunite and reconcile, but Subaru apologizes, saying that he could not keep his promise and was unable to protect him, Gaou, who has gained the ability to speak through the Mirror Stone.
