Jimmy Fallon was originally supposed to take over when Jay Leno's contract expired in September 2014. However, Leno suggested that Fallon take over earlier in February, to take advantage of the ratings for the 2014 Winter Olympics.
During the opening theme sequence, just as Jimmy Fallon comes through the curtain, The Roots drummer Questlove calls out the episode number for that show.
After mentioning a one-eyed rockfish with a glass eye at the Vancouver Aquarium, the aquarium named the fish "Jimmy", in Jimmy Fallon's honor.
The show's studio, NBC Studio 6B, was the same studio from which The Tonight Show Starring Johnny Carson (1962) was originally broadcast. This was the first time "The Tonight Show" was regularly broadcast from New York City since Carson moved the show to Burbank in 1972.