Biotin Antibody (33): sc-101339
- Biotin Antibody (33) is a mouse monoclonal IgG1 κ, cited in 19 publications, provided at 200 µg/ml
- raised against Biotin
- Biotin Antibody (33) is recommended for detection of both free and carrier protein bound biotin by WB, IP and IF; also recommended for detection of biocytin
- Anti-Biotin Antibody (33) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Biotin (33): sc-101339.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Biotin Antibody (33) for WB applications. These reagents are now offered in bundles with Biotin Antibody (33) (see ordering information below).
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Biotin Antibody (33) is a mouse monoclonal IgG1 kappa light chain antibody that detects Biotin in non-species reactive applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Biotin antibody (33) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Biotin, a vital water-soluble B complex vitamin, plays a crucial role in numerous metabolic processes, particularly in carboxylation reactions essential for synthesizing fatty acids, metabolizing leucine, and facilitating gluconeogenesis. Biotin′s unique structure, characterized by a tetrahydrothiophene ring fused with an ureido ring, allows Biotin to serve as a cofactor for carboxylase enzymes, which are pivotal in energy metabolism and biosynthetic pathways. While Biotin is synthesized by certain microorganisms and plants, Biotin is also produced in sufficient quantities by human intestinal bacteria to meet daily requirements; however, a deficiency, though rare, can lead to significant health issues such as dermatitis, hair loss, and neurological symptoms. Anti-Biotin antibody (33) serves as an essential tool for researchers studying metabolic pathways and biotin-related functions in various biological systems.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Biotin Antibody (33) References:
- Skin manifestations of biotin deficiency. | Mock, DM. 1991. Semin Dermatol. 10: 296-302. PMID: 1764357
- Analysis of bacterial biotin-proteins. | Fall, RR., et al. 1975. Biochim Biophys Acta. 379: 496-503. PMID: 235315
- Biotin Treatment Mimicking Graves' Disease. | Kummer, S., et al. 2016. N Engl J Med. 375: 704-6. PMID: 27532849
- Chemical Modification of Proteoglycanases with Biotin. | Santamaria, S. 2020. Methods Mol Biol. 2043: 113-123. PMID: 31463907
- Chemistry of Biotin-Streptavidin and the Growing Concern of an Emerging Biotin Interference in Clinical Immunoassays. | Luong, JHT. and Vashist, SK. 2020. ACS Omega. 5: 10-18. PMID: 31956746
- Advances and prospects in microbial production of biotin. | Ma, D., et al. 2024. Microb Cell Fact. 23: 135. PMID: 38735926
- Biotin status of epileptics. | Krause, KH., et al. 1985. Ann N Y Acad Sci. 447: 297-313. PMID: 3925859
- Biotin-mediated protein biosynthesis. | Boeckx, RL. and Dakshinamurti, K. 1974. Biochem J. 140: 549-56. PMID: 4447630
- Biotinidase and its roles in biotin metabolism. | Hymes, J. and Wolf, B. 1996. Clin Chim Acta. 255: 1-11. PMID: 8930409
- Biotin-conjugated polychelating agent. | Torchilin, VP. 1999. Bioconjug Chem. 10: 146-9. PMID: 9893977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Biotin Antibody (33) | sc-101339 | 200 µg/ml | $316.00 | |||
Biotin Antibody (33): m-IgG Fc BP-HRP Bundle | sc-528715 | 200 µg Ab; 10 µg BP | $354.00 | |||
Biotin Antibody (33): m-IgGκ BP-HRP Bundle | sc-521186 | 200 µg Ab, 40 µg BP | $354.00 | |||
Biotin Antibody (33): m-IgG1 BP-HRP Bundle | sc-543085 | 200 µg Ab; 20 µg BP | $354.00 | |||
Biotin Antibody (33) AC | sc-101339 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Biotin Antibody (33) HRP | sc-101339 HRP | 200 µg/ml | $316.00 | |||
Biotin Antibody (33) FITC | sc-101339 FITC | 200 µg/ml | $330.00 | |||
Biotin Antibody (33) PE | sc-101339 PE | 200 µg/ml | $343.00 | |||
Biotin Antibody (33) Alexa Fluor® 488 | sc-101339 AF488 | 200 µg/ml | $357.00 | |||
Biotin Antibody (33) Alexa Fluor® 546 | sc-101339 AF546 | 200 µg/ml | $357.00 | |||
Biotin Antibody (33) Alexa Fluor® 594 | sc-101339 AF594 | 200 µg/ml | $357.00 | |||
Biotin Antibody (33) Alexa Fluor® 647 | sc-101339 AF647 | 200 µg/ml | $357.00 | |||
Biotin Antibody (33) Alexa Fluor® 680 | sc-101339 AF680 | 200 µg/ml | $357.00 | |||
Biotin Antibody (33) Alexa Fluor® 790 | sc-101339 AF790 | 200 µg/ml | $357.00 |