PLAP Antibody (8B6): sc-47691

PLAP Antibody (8B6) is a mouse monoclonal IgG2a kappa light chain antibody that detects the PLAP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). PLAP (8B6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The placental alkaline phosphatase (PLAP) protein, also known as ALPP, is a 530 amino acid glycoprotein that plays a crucial role in cellular processes by catalyzing the hydrolysis of phosphomonoesters into inorganic phosphate and alcohol, which is vital for various metabolic pathways. PLAP is primarily expressed in the placenta and is also found in the serum of pregnant women, with ectopic expression observed in certain cancers, such as ovarian and testicular tumors. PLAP expression in these tissues suggests involvement in guiding migratory cells and facilitating the transport of specific molecules, including fatty acids and immunoglobulins, across the plasma membrane, which is essential for maintaining cellular homeostasis and supporting tumor progression. Notably, PLAP, along with germ cell alkaline phosphatase (GCAP) and intestinal alkaline phosphatase (IAP), are tissue-specific alkaline phosphatases that share 90-98% homology and are clustered on chromosome 2q, highlighting their evolutionary relationship and functional significance in human physiology.

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PLAP Antibody (8B6) References:

1. Crystal structure of alkaline phosphatase from human placenta at 1.8 A resolution. Implication for a substrate specificity.  |  Le Du, MH., et al. 2001. J Biol Chem. 276: 9158-65. PMID: 11124260
2. Placental-type alkaline phosphatase in peritoneal fluid of women with endometriosis.  |  Kang, JO., et al. 1990. Clin Chim Acta. 186: 285-94. PMID: 2311256
3. Placental alkaline phosphatase-like isoenzymes produced by human gastric cancer cells.  |  Aizawa, K., et al. 1989. Acta Pathol Jpn. 39: 630-7. PMID: 2686348
4. Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer.  |  Fisken, J., et al. 1989. J Clin Pathol. 42: 40-5. PMID: 2921344
5. Serum marker potential of placental alkaline phosphatase-like activity in testicular germ cell tumours evaluated by H17E2 monoclonal antibody assay.  |  Tucker, DF., et al. 1985. Br J Cancer. 51: 631-9. PMID: 2986664
6. Diagnostic aspects of alkaline phosphatase and its isoenzymes.  |  Moss, DW. 1987. Clin Biochem. 20: 225-30. PMID: 3319285
7. Human placental and intestinal alkaline phosphatase genes map to 2q34-q37.  |  Griffin, CA., et al. 1987. Am J Hum Genet. 41: 1025-34. PMID: 3687940
8. Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours.  |  Epenetos, AA., et al. 1985. Br J Cancer. 51: 641-4. PMID: 3994908
9. Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants.  |  Travers, P. and Bodmer, W. 1984. Int J Cancer. 33: 633-41. PMID: 6202646
10. An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.  |  Epenetos, AA., et al. 1984. Br J Cancer. 49: 11-5. PMID: 6362705
11. Biology of human alkaline phosphatases with special reference to cancer.  |  Millán, JL. and Fishman, WH. 1995. Crit Rev Clin Lab Sci. 32: 1-39. PMID: 7748466
12. Profile of placental alkaline phosphatase expression in human malignancies: effect of tumour cell activation on alkaline phosphatase expression.  |  Dabare, AA., et al. 1999. Urol Int. 63: 168-74. PMID: 10738188

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PLAP Antibody (8B6) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-PLAP Antibody (8B6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PLAP Antibody (8B6) Specifications

Alternate Product Name: PLAP

Product Clone Name: 8B6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: PLAP

Epitope Species: human

Product Reactivity/Detection: PLAP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for PLAP Antibody (8B6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred TNAP antibody is TNAP Antibody (F-4): sc-166261.