1785213364
Promiscuity in Alkaline Phosphatase Superfamily. Unraveling Evolution through Molecular Simulations
J. Am. Chem. Soc., 2011
We here present a theoretical study of the alkaline hydrolysis of a phosphodiester (methyl p-nitrophenyl phosphate or MpNPP) in the active site of Escherichia coli alkaline phosphatase (AP), a monoesterase that also presents promiscuous activity as a diesterase. The analysis of our simulations, carried out by means of molecular dynamics (MD) simulations with hybrid quantum mechanics/molecular mechanics (QM/MM) potentials, shows that the reaction takes place through a DNAN or dissociative mechanism, the same mechanism employed by AP in the hydrolysis of monoesters. The promiscuous activity observed in this superfamily can be then explained on the basis of a conserved reaction mechanism. According to our simulations the specialization in the hydrolysis of phos...
1785213364
Numerical Study and Experimental Validation of Deformation of <111> FCC CuAl Single Crystal Obtained by Additive Manufacturing
Metals, 2021
The importance of taking into account directional solidification of grains formed during 3D printing is determined by a substantial influence of their crystallographic orientation on the mechanical properties of a loaded material. This issue is studied in the present study using molecular dynamics simulations. The compression of an FCC single crystal of aluminum bronze was performed along the <111> axis. A Ni single crystal, which is characterized by higher stacking fault energy (SFE) than aluminum bronze, was also considered. It was found that the first dislocations started to move earlier in the material with lower SFE, in which the slip of two Shockley partials was observed. In the case of the material with higher SFE, the slip of a full dislocation...
1785213364
Computer game for inner-city children does not improve asthma outcomes
Journal of Pediatric Health Care, 2003
The purpose of this randomized control trial was to evaluate the effects of a computer-assisted instructional (CAI) game on asthma symptoms (eg, coughing, wheezing, shortness of breath, and nighttime awakenings) in 7- to 12-year-old inner-city children over 12 weeks.A CAI that focused on reducing environmental irritants/allergens and the correct use of prescribed medications to prevent asthma symptoms was used with 101 children (56 in the intervention group and 45 in the control group). The primary outcome evaluated a change in children's asthma symptoms as measured by responses to nine symptom questions in Juniper's Pediatric Asthma Quality of Life Questionnaire (PAQOL) and measurements of lung function.No significant changes in asthma symptoms occurred bet...
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Regulation of UDP-glucuronosyltransferase (UGT) 1A1 by progesterone and its impact on labetalol elimination
Xenobiotica, 2008
The authors recently reported the increased oral clearance of labetalol in pregnant women. To elucidate the mechanism of the elevated oral clearance, it was hypothesized that female hormones, at the high concentrations attainable during pregnancy, enhance hepatic metabolism of labetalol. Labetalol glucuronidation, which is the major elimination pathway of labetalol, was characterized by screening six recombinant human UGTs (UGT1A1, 1A4, 1A6, 1A9, 2B4, and 2B7) for their capacity to catalyse labetalol glucuronidation. The effect of female hormones (progesterone, oestradiol, oestriol, or oestrone) on the promoter activities of relevant UDP glucuronosyltransferases (UGT) was investigated using a luciferase reporter assay in HepG2 cells. The involvement of oestr...
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Effects of Oxygen on Cell Turnover and Expression of Regulators of Apoptosis in Human Placental Trophoblast
Placenta, 2008
Pre-eclampsia (PE) and intrauterine growth restriction (IUGR) are associated with aberrant cell turnover, including increased apoptosis, in placental villous trophoblast. The increased apoptosis is associated with exaggerated expression of p53, which promotes cell cycle arrest or apoptosis via downstream proteins such as p21 or Bax. These changes in apoptosis and p53 expression are purported to result from exposure to altered oxygen tension. Using a model of villous trophoblast turnover, we examined the effect of 20%, 6% and 1% ambient oxygen (O(2)) on apoptosis, necrosis, proliferation and expression of p53 and related regulators of cell turnover, compared to both fresh tissue. Altered O(2) tension exerted an effect on cell turnover in cultured term villous...
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Cloning and characterization of the promoter of Hugl-2, the human homologue of Drosophila lethal giant larvae (lgl) polarity gene
Biochemical and Biophysical Research Communications, 2008
The human lgl gene, Hugl-2 (llgl2, Lgl2), codes for a cytoskeletal protein involved in regulating cell polarity. Here, we report the identification and functional characterization of the promoter region (∼1.2 kb) of the Hugl-2 gene. Luciferase expression assays show a high basal Hugl-2 promoter activity in different cell lines and primary human hepatocytes. Truncations of the promoter identified a GC-rich region important for this activity. Alignment of human and mouse genomic sequences demonstrate that this is an evolutionary conserved region fcontaining putative binding sites for several transcription factors including Elk-1 and Sp-1. Mithramycin A reduces Hugl-2 expression indicating Sp-1 transcription factors activate Hugl-2. Treatment of primary hepat...
1785213363
Solubilities of Some Chlorophenols in Supercritical CO2 in the Presence and Absence of Cosolvents
J. Chem. Eng. Data, 2009
The solubilities of three chlorophenols, namely, 4-chlorophenol, 2,4-dichlorophenol, and 2,4,6-trichlorophenol, in supercritical carbon dioxide were determined at temperatures from (308 to 318) K in the pressure range of (8.8 to 15.6) MPa. The solubilities were determined both in the absence of cosolvents and in the presence of two cosolvents, methanol and acetone. The solubilities (in the absence of cosolvents) in mole fraction of 4-chlorophenol, 2,4-dichlorophenol, and 2,4,6-trichlorophenol at 308 K were in the range of (0.0113 to 0.0215), (0.0312 to 0.0645), and (0.008 to 0.0173), respectively. The solubilities of the chlorophenols followed the order 2,4-dichlorophenol > 4-chlorophenol > phenol > 2,4,6-trichlorophenol > pentachlorophenol. The solubility d...
1785213363
A Novel Transverse-Flux Moving-Magnet Linear Oscillatory Actuator
IEEE Trans. Magn., 2012
A novel transverse-flux moving-magnet linear oscillatory actuator (TMLOA) for a compressor is proposed and analyzed, which features a unique configuration composed of usual laminated stators and moving-magnet mover. It not only offers low manufacturing cost and great dynamic characteristics, but also provides high thrust force density for its transverse flux path. The magnetic field and thrust force are analyzed by three-dimensional finite-element model. The proposed model is also used for studying effects of the axial dimensions variation on the thrust force and stroke, and then the selection of axial parameters is obtained with reference to the amplitude and smoothness of thrust force. Finally, the measurements of a prototype validate the predicted results...
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Ensuring Benevolent Neutrality: The British Government's Appeasement of General Franco during the Spanish Civil War, 1936–1939
The International History Review, 2018
This article examines the development of British non-intervention in the Spanish Civil War (1936–39). Previous studies have focused heavily on pro-rebel or anti-Republican sentiments among British officials in London and abroad, and often apply the term ‘malevolent neutrality’ to the motives behind the policy. However, utilising records from the National Archives as well as private papers, this article evaluates British non-intervention within the context of appeasement and demonstrates a clear link between the two policies. By examining British neutrality through the lens of appeasement, this study will enhance our understanding of British diplomacy in the 1930s and the links between non-intervention in Spain and the growing threat of fascism in Europ...
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Vehement emotions and trauma-generated dissociation: A Janetian perspective on integrative failure
European Journal of Trauma & Dissociation, 2019
Abstract As the construct trauma-generated dissociation has received a wide range of meanings in recent years, it may be fruitful to re-visit Pierre Janet's original views. Having been the first researcher to study this phenomenon in depth, he concluded that so-called vehement emotions, that is, those that arise when a major challenge is beyond the individual's capacity for efficient and effective action, entail an integrative failure that involves a disaggregation or dissociation of the personality, especially as part of traumatic experiences. This involves a division of the personality into different subsystems, each with its own sense of self and first-person perspective, manifesting in a range of positive and negative dissociative symptoms. This dissocia...
1785213362
Evaluation of shrinkage and cracking in concrete of ring test by acoustic emission method
Mod. Phys. Lett. B, 2015
Drying shrinkage of concrete is one of the typical problems related to reduce durability and defilation of concrete structures. Lime stone, expansive additive and low-heat Portland cement are used to reduce drying shrinkage in Japan. Drying shrinkage is commonly evaluated by methods of measurement for length change of mortar and concrete. In these methods, there is detected strain due to drying shrinkage of free body, although visible cracking does not occur. In this study, the ring test was employed to detect strain and age cracking of concrete. The acoustic emission (AE) method was adopted to detect micro cracking due to shrinkage. It was recognized that in concrete using lime stone, expansive additive and low-heat Portland cement are effective to decrease...
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Antihypertensive Indigenous Lebanese Plants: Ethnopharmacology and a Clinical Trial
Biomolecules, 2019
Hypertension is highly prevalent among the Lebanese adult population and is indeed the major cause of mortality in Lebanon. Traditional use of antihypertensive medicinal plants has long been practiced. The aim of this study is to document this traditional knowledge and clinically test the antihypertensive capacity of three of the most commonly used wild plant species Mentha longifolia, Viola odorata and Urtica dioica. Ethno-pharmacological data was collected by personal interviews with herbalists and traditional healers using a semi structured survey questionnaire and assessing relative frequency of citation (RFC). The clinical study was conducted by a randomized, blind, placebo-controlled trial in 29 subjects with mild hypertension distributed in four group...
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Shaping Parliamentary Democracy: Collected Memories from the European Parliament, edited by A.De Feo and M.Shackleton (London: Palgrave Macmillan, 2019, ISBN 9783030272128); xi+135pp., €52.00 hb.
J of Common Market Studies, 2020
JCMS: Journal of Common Market StudiesVolume 58, Issue 3 p. 780-781 Book Review Shaping Parliamentary Democracy: Collected Memories from the European Parliament, edited by A. De Feo and M. Shackleton ( London: Palgrave Macmillan, 2019, ISBN 9783030272128); xi+135pp., €52.00 hb. Anja Thomas, Anja Thomas European University InstituteSearch for more papers by this author Anja Thomas, Anja Thomas European University InstituteSearch for more papers by this author First published: 28 April 2020 https://doi.org/10.1111/jcms.13038Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share f...
1785213362
Outcomes of laparoscopic hepatic resection versus percutaneous radiofrequency ablation for hepatocellular carcinoma located at the liver surface: A case-control study with propensity score matching
Hepatol Res, 2015
Aim Percutaneous radiofrequency ablation (P-RFA) therapy is a widely applied treatment for small hepatocellular carcinoma (HCC); however, local recurrence is a major issue of HCC located at the surface of the liver (surface HCC). The aim of this study was to compare the outcome of laparoscopic hepatic resection (LH) and P-RFA for surface HCC in case–control patient groups using the propensity score. Methods Between 2011 and 2013, 40 and 52 patients underwent LH and P-RFA for surface HCC (≤3 cm, 1–3 nodules). To correct the difference in clinicopathological factors between the two groups, propensity score matching was used at a 1:1 ratio, which resulted in a comparison of 27 patients/group. We compared outcomes between the two groups, with special refer...
1785213362
Molecular weight distribution and environmental stress cracking of linear polyethylene
Polymer Engineering & Sci, 1966
Molecular weight distributions of both Phillips and Zieglertype high density polyethylenes were determined following fractionation of the polymer samples using a column elution technique. The Wesslau log normal distribution function was used to describe the distributions of the resins investigated. Resistance to environmental stress rupture of speciments cut from compression molded plaques of these samples was measured by the constant tensile loading procedure. Data are presented showing annealed resins with “broad” molecular weight distributions, characterized particularly by a quantity of low molecular weigt material and a high molecular weight “tail,” to have poorer stress crack resistance than samples having a “narrow” molecular weight distri...
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Plosive (de-)voicing and f0 perturbations in Tokyo Japanese: Positional variation, cue enhancement, and contrast recovery
Journal of Phonetics, 2019
This study addresses the two-way laryngeal contrast of plosives in Tokyo Japanese, which is commonly analyzed as a “true voicing” language. We examine how voicing-related properties of the plosive and f0 of the following vowel varied with the position in the word and in the sentence. We compare word-initial with word-medial positions for words in citation (between two pauses) and for two prosodic conditions in a carrier sentence: with vs. without a preceding pause. In word-initial position, unlike in a typical “true-voicing” language such as French, voiced plosives in Tokyo Japanese show a high devoicing rate, while voiceless plosives are moderately aspirated. A combination of VOT and f0 of the following vowel is used to distinguish the two plosive s...
1785213361
PPARγ-dependent and -independent effects of Rosiglitazone on lipotoxic human pancreatic islets
Biochemical and Biophysical Research Communications, 2008
We explored the in vitro effects of Rosiglitazone (RZG), a PPARγ agonist, on human pancreatic islet dysfunctions induced by chronic free fatty acid exposure. We demonstrated that RZG beneficial effects on insulin secretion and apoptosis did not imply PDX-1 or insulin gene modulation. It rather involved, through a PPARγ-dependent mechanism, a reduction of iNOS overexpressed in lipotoxic islets. This reduction likely led to the restoration of ATP level and insulin secretion as well as the decrease in apoptosis. More interestingly, we also demonstrated that RZG beneficial effects involved PPARγ-independent mechanisms. RZG treatment led to a limitation of oxidative stress exemplified by an increase of GPx and SOD expression. It also increased UCP2 expression ...
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Temporal lobe epilepsy in The Passion According to GH
The Lancet Neurology, 2018
Clarice Lispector was an iconic Brazilian writer who was known for her strange, almost mystical writings. Her novel, The Passion According to GH, 1 Lispector C The Passion according to GH. University Of Minnesota Press, Minneapolis, MN1988 Google Scholar has long been considered to describe the internal workings of a woman (GH), who suddenly and unexpectedly finds herself in the midst of a spiritual crisis that is triggered by the sight of a cockroach in a clean room. During her crisis, the protagonist reports feeling disoriented and irritated, with subsequent auditory and visual hallucinations, and a range of symptoms including screaming, dry mouth, clenching of the jaw, intense sweating, and sudden vomiting. Additionally, the protagonist experiences a feel...
1785213361
Source Identification and Behavior of PCDD/Fs and Dioxin-like PCBs in Japanese River Water
Journal of Japan Society on Water Environment, 2003
The result of source identification of polychlorinated dibenzo-p-dioxins, dibenzofurans (PCDD/Fs) and dioxin-like PCBs (co-PCBs) in Japanese river water is presented for the first time. River water and atmospheric deposition samples were collected from different locations in Tokyo Metropolis and Kanagawa Prefecture. The river water samples exhibited a range from 190 to 520 pg·l-1 for total mono through octachlorinated PCDD/Fs and a range from 21 to 220 pg·l-1 for total co-PCBs. Dichlorinated dibenzo-p-dioxins (DiCDDs) were the most abundant homologue in the river water samples (54-89% of total PCDD/Fs), while monochlorinated dibenzofurans (MoCDFs) were the most abundant homologue in the atmospheric deposition samples (24-51% of total PCDD/Fs). Th...
1785213361
Differential Recognition of Phosphorylated Transactivation Domains of p53 by Different p300 Domains
Journal of Molecular Biology, 2008
Histone acetyltransferases form crucial links in transducing extrinsic signals to actual initiation of transcription. A multitude of stress signal integrations occur through the interaction of p300 with p53 phosphorylated at different residues of the transactivation domain. How such interactions activate different gene expression programs remains largely unknown. p300 contains at least five domains that are known to interact with p53, but their role in transcription regulation is not known. We measured the binding affinity of various phosphorylated transactivation domains towards several p53 binding domains of p300 by fluorescence anisotropy. The binding affinities of different phosphorylated transactivation domains of p53 towards different domains of p300 v...
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Methodologies and Algorithms for Group-Rankings Decision
Management Science, 2006
The problem of group ranking, also known as rank aggregation, has been studied in contexts varying from sports, to multicriteria decision making, to machine learning, to ranking Web pages, and to behavioral issues. The dynamics of the group aggregation of individual decisions has been a subject of central importance in decision theory. We present here a new paradigm using an optimization framework that addresses major shortcomings that exist in current models of group ranking. Moreover, the framework provides a specific performance measure for the quality of the aggregate ranking as per its deviations from the individual decision-makers’ rankings. The new model for the group-ranking problem presented here is based on rankings provided with intensity—that...
1785213361
Generation of an Adjustable Optical Cage through Focusing an Apertured Bessel-Gaussian Correlated Schell-Model Beam
Applied Sciences, 2019
An adjustable optical cage generated by focusing a partially coherent beam with nonconventional correlation function named the Bessel–Gaussian correlated Schell-model (BGCSM) beam is investigated in detail. With the help of the generalized Huygens–Fresnel integral and complex Gaussian function expansion, the analytical formula of the BGCSM beam passing through an apertured ABCD optical system was derived. Our numerical results show that the generated optical cage can be moderately adjusted by the aperture radius, the spatial coherence width, and the parameter β of the BGCSM beam. Furthermore, the effect of these parameters on the effective beam size and the spectral degree of coherence were also analyzed. The optical cage with adjustable size can be app...
1785213361
Chemical compositions and yield of essential oil of Moldavian balm (Dracocephalum moldavica L.) in intercropping with faba bean (Vicia faba L.) under different fertilizers application
Journal of Cleaner Production, 2019
Organic fertilizers and intercropping with legumes can be used as alternatives for chemical fertilizers in sustainable production. In order to evaluate the effects of chemical fertilizer (CF), bio-fertilizer (BF) and vermicompost (VC) on essential oil content, yield and chemical compositions of Moldavian balm (MB) in sole crop and intercropping with faba bean (FB) a field study was carried out in Iran in two growth seasons. Results indicated that the interaction effect of cropping pattern × fertilizer was significant on dry herbage yield of Moldavian balm (P ≤ 0.05) and among the intercropping patterns the highest values were obtained in FB:MB 2:2 intercropping. Essential oil content affected by fertilizer treatment and the highest value (0.57%) was obser...
1785213361
A comprehensive, multiscale framework for evaluation of arrhythmias arising from cell therapy in the whole post-myocardial infarcted heart
Sci Rep, 2019
Direct remuscularization approaches to cell-based heart repair seek to restore ventricular contractility following myocardial infarction (MI) by introducing new cardiomyocytes (CMs) to replace lost or injured ones. However, despite promising improvements in cardiac function, high incidences of ventricular arrhythmias have been observed in animal models of MI injected with pluripotent stem cell-derived cardiomyocytes (PSC-CMs). The mechanisms of arrhythmogenesis remain unclear. Here, we present a comprehensive framework for computational modeling of direct remuscularization approaches to cell therapy. Our multiscale 3D whole-heart modeling framework integrates realistic representations of cell delivery and transdifferentiation therapy modalities as well as re...
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Biochemical and functional characterization of a new murine monoclonal antibody against human platelet glycoprotein IIIa
Platelets, 1996
A new murine monoclonal antibody, MDP-1, specific for human platelet glycoprotein IIIa has been produced and characterized. Following SDS-polyacrylamide gel electrophoresis, MDP-1 reacted with a 94kDa protein immobilized on a nitrocellulose membrane. Upon reduction, MDP-1 no longer bound to the 94kDa protein indicating an epitope requiring at least one disulfide bond. On crossed immunoelectrophoresis MDP-1 reacted to the same peak as the GP IIb-IIIa complex-specific antibody AP-2. After dissociation of the GP IIb-IIIa complex with EDTA, AP-2 showed no reactivity while MDP-1 bound to a new peak that was broader and anodal to the original GP IIb-IIIa peak, consistent with GP IIIa. MDP-1 inhibited ADP and thrombin induced aggregation. In addition, MDP-1 inhibit...
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A self-administered screener for migraine in primary care
Neurology, 2003
Background: Migraine is a highly prevalent and disabling illness that remains substantially undiagnosed in primary care. Because of the potential value of a screening tool, the current study was designed to establish the validity and reliability of a brief, self-administered migraine screener in patients with headache complaints in the primary care setting. Methods: A total of 563 patients presenting for routine primary care appointments and reporting headaches in the past 3 months completed a self-administered migraine screener. All patients were then referred for an independent diagnostic evaluation by a headache expert, of whom 451 (80%) completed a full evaluation. Migraine diagnosis was assigned based on International Headach...
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A retrospective survey of Brucella melitensis human infection in Hainan Province, China
Biosafety and Health, 2021
Abstract In China, brucellosis has spread from the northern provinces to the southern coastal area, and Brucella melitensis has become the overwhelmingly dominant species nationwide. Although human Brucellosis cases have been reported in many parts of Hainan Province, investigations into the source of infection have been rare. In this case, we have used the techniques of bacteriology, genome sequencing, and WGS-SNP to investigate the source of infection on a patient with a traveling history between Hainan and Inner Mongolia autonomous regions. We collected a 5mL blood sample under sterile conditions from this suspected case of Brucellosis infection and incubated it in a culture bottle. A week later, Brucella colonies were purified and identified under the st...
1785213360
Collective goods and collective decision mechanisms
Public Choice, 1973
Economists stress the role of differences in relative prices (or costs) in explaining observed behavior. However, the full potential of this type of analysis for clarifying a number of fundamental socio-political issues involving (or public) goods has not yet been fully realized. With scattered but notable exceptions,1 writers in the area have focused on what should be, neglecting the question of more traditional scientific interest of what will be. We propose to provide a step towards the development of a complete comparative statics of collective goods at the same time we begin to explore some of its implications. To start with, the notion of a collective good is placed in the context of some recent advances in consumer theory. Part I then examines the fac...
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Elevated “Hydroxyl Radical” Generation In Vivo in an Animal Model of Amyotrophic Lateral Sclerosis
Journal of Neurochemistry, 1998
Mutations in the enzyme copper/zinc superoxide dismutase-1 (SOD1) are associated with familial amyotrophic lateral sclerosis (FALS). The means by which the mutations cause FALS appears to be due to an adverse property of the mutant SOD1 protein that may involve increased generation of free radicals. We used in vivo microdialysis to measure the conversion of 4-hydroxybenzoic acid to 3,4-dihydroxybenzoic acid (3,4-DHBA) as a measure of "hydroxyl radical-like" production in transgenic amyotrophic lateral sclerosis (ALS) mice with the G93A mutation as well as littermate controls. The conversion of 4-hydroxybenzoic acid to 3,4-DHBA was significantly increased in the striatum of transgenic ALS mice at baseline but not in mice overexpressing wild-type human SOD1. F...
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Behaviour of concrete in a stress continuity region after cracking under restrained drying shrinkage
Structural Concrete, 2013
Some studies have investigated the time-dependent characteristic of concrete for predicting stress under restrained shrinkage. However, these studies investigated the shrinkage behaviour of concrete in restrained conditions before cracking, and no study has yet investigated the behaviour of concrete due to restrained shrinkage in the stress continuity region after cracking. Therefore, this study investigates the shrinkage behaviour of concrete in the stress continuity region after cracking in uniaxial restrained specimens and analyses the restrained tensile stress of concrete in this region by way of a bond analysis. The results show that the effective tensile Young's modulus considering tensile creep is approx. 22–26% of the static modulus of elasticity. ...
1785213360
Antifungal properties and inhibitory effects upon aflatoxin production by Zingiber officinale essential oil in Aspergillus flavus
Int J of Food Sci Tech, 2015
Summary
In this study, the efficacy of ginger (Zingiber officinale Roscoe) essential oil (GEO) in reducing A. flavus growth and aflatoxin production was investigated. Gas chromatography coupled to mass spectrometry and nuclear magnetic resonance spectroscopy showed that the major components of GEO were α-zingiberene (23.85%) and geranial (14.16%). Mycelial growth of Aspergillus flavus was reduced significantly at a GEO concentration of 150 μg mL−1, and complete inhibition of conidial germination was observed at a concentration of 10 μg mL−1. Statistically significant inhibition of ergosterol biosynthesis was detected at a GEO concentration of 10 μg mL−1. GEO was capable of fully inhibiting aflatoxin production by A. flavus at a concentratio...
1785213360
Regulation of the murine TR2/HVEM gene expression by IRF
Biochemical and Biophysical Research Communications, 2008
TR2 (TNFR-related 2, HVEM, or TNFRSF-14), a member of the TNFR family, is involved in a number of immune responses. While TR2 is expressed on the surface of T cells during the resting state, little is known regarding how expression of the TR2 gene is regulated. To understand the mechanisms regulating the expression of TR2 in T cells, we analyzed the 5’ flanking region of TR2. We identified an important region for the activity of the TR2 promoter using site directed mutagenesis. Using EMSA analysis, we found that IRF-2 was bound to the promoter region of the TR2 gene during the resting state of EL-4 T cells. Transfection of IRF-2 expression plasmid and of dominant negative IRF-2 mutant further confirmed our results. Together, these data demonstrate that IRF...
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Live-cell multiphoton fluorescence correlation spectroscopy with an improved large Stokes shift fluorescent protein
MBoC, 2015
We report an improved variant of mKeima, a monomeric long Stokes shift red fluorescent protein, hmKeima8.5. The increased intracellular brightness and large Stokes shift (∼180 nm) make it an excellent partner with teal fluorescent protein (mTFP1) for multiphoton, multicolor applications. Excitation of this pair by a single multiphoton excitation wavelength (MPE, 850 nm) yields well-separable emission peaks (∼120-nm separation). Using this pair, we measure homo- and hetero-oligomerization interactions in living cells via multiphoton excitation fluorescence correlation spectroscopy (MPE-FCS). Using tandem dimer proteins and small-molecule inducible dimerization domains, we demonstrate robust and quantitative detection of intracellular protein–protein int...
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Estimation of mating system parameters in an evolving gynodioecous population of cultivated sunflower (Helianthus annuus L.)
Heredity, 2011
Cultivated plants have been molded by human-induced selection, including manipulations of the mating system in the twentieth century. How these manipulations have affected realized parameters of the mating system in freely evolving cultivated populations is of interest for optimizing the management of breeding populations, predicting the fate of escaped populations and providing material for experimental evolution studies. To produce modern varieties of sunflower (Helianthus annuus L.), self-incompatibility has been broken, recurrent generations of selfing have been performed and male sterility has been introduced. Populations deriving from hybrid-F1 varieties are gynodioecious because of the segregation of a nuclear restorer of male fertility. Using both ph...
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Light emitting composite beams during matrix cracking
Journal of Composite Materials, 2017
Defects in fiber-reinforced composite structures tend to initiate unpredictably and unalarmed due to local stress concentrations within a composite structure; this has given rise to active monitoring techniques that can quantify the mechanical stress within composites in order to evaluate the structural health. In this paper, triboluminescent mechanisms are used for damage monitoring of composite matrix under flexural loading. Vinyl ester resin is doped with ZnS:Mn phosphors and reinforced with glass fiber whiskers, were subjected to flexural loading while observing both the triboluminescent and acoustic response using a photo multiplier tube (PMT) and micro-mic respectively. Validity of triboluminescent emissions for determining structural integrity of glas...
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The Free Energy Landscape of Pseudorotation in 3′–5′ and 2′–5′ Linked Nucleic Acids
J. Am. Chem. Soc., 2014
The five-membered furanose ring is a central component of the chemical structure of biological nucleic acids. The conformations of the furanose ring can be analytically described using the concept of pseudorotation, and for RNA and DNA they are dominated by the C2'-endo and C3'-endo conformers. While the free energy difference between these two conformers can be inferred from NMR measurements, a free energy landscape of the complete pseudorotation cycle of nucleic acids in solution has remained elusive. Here, we describe a new free energy calculation method for molecular dynamics (MD) simulations using the two pseudorotation parameters directly as the collective variables. To validate our approach, we calculated the free energy surface of ribose pseudorotati...
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A New Approximate Equation for Heat Capacities at High Temperatures
J. Am. Chem. Soc., 1935
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA New Approximate Equation for Heat Capacities at High TemperaturesJohn Chipman and M. G. FontanaCite this: J. Am. Chem. Soc. 1935, 57, 1, 48–51Publication Date (Print):January 1, 1935Publication History Published online1 May 2002Published inissue 1 January 1935https://doi.org/10.1021/ja01304a015RIGHTS & PERMISSIONSArticle Views44Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated dail...
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External dose assessment for workers dismantling the bio-shield of a commercial power nuclear reactor: Case study of Kori-1, Korea
Nuclear Engineering and Technology, 2020
Abstract The license for Kori-1, the first commercial reactor in Busan, Korea, was terminated in June 2017; therefore, preparations are being made for its decommissioning. Because the radioactivity of Bio-shield varies greatly throughout the structure, the doses received by the workers depend on the location, order, and duration of dismantling operations. Thus, a model for evaluating the worker external dose during the dismantling of the Kori-1 bio-shield was developed, and work scenarios for dose assessment were designed. The Dose evaluation code VISIPLAN was used for dose assessment. The dose rate around the bio-shield was evaluated and the level of exposure to the operator was evaluated according to the work scenario. The maximum annual external dose was ...
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Culture, Hybridity, and the Dialogical Self: Cases From the South Asian Diaspora
Mind, Culture, and Activity, 2004
This article outlines a dialogical approach to understanding how South Asian-American women living in diasporic locations negotiate their multiple and often conflicting cultural identities. We specifically use the concept of voice to articulate the different forms of dialogicality-polyphonization, expropriation, and ventriloquation-that are involved in the acculturation experiences of two 2nd-generation South Asian-American women. In particular, we argue that it is important to think of acculturation of the South Asian-American women as essentially a contested, dynamic, and dialogical process. We demonstrate that such a dialogical process involves a constant moving back and forth between various cultural voices that are connected to various sociocultural con...
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Functional analyses of the C-terminal half of the Saccharomyces cerevisiae Rad52 protein
Nucleic Acids Research, 2013
The Saccharomyces cerevisiae Rad52 protein is essential for efficient homologous recombination (HR). An important role of Rad52 in HR is the loading of Rad51 onto replication protein A-coated single-stranded DNA (ssDNA), which is referred to as the recombination mediator activity. In vitro, Rad52 displays additional activities, including self-association, DNA binding and ssDNA annealing. Although Rad52 has been a subject of extensive genetic, biochemical and structural studies, the mechanisms by which these activities are coordinated in the various roles of Rad52 in HR remain largely unknown. In the present study, we found that an isolated C-terminal half of Rad52 disrupted the Rad51 oligomer and formed a heterodimeric complex with Rad51. The Rad52 fragment ...
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Absorption of Slow Neutrons in Silver
Phys. Rev., 1935
Fermi and his collaborators [1] have measured the absorption of slow neutrons in a variety of elements using as a detector of the neutrons usually rhodium, and sometimes silver. Wide differences are found among the various elements tested, and the removal of neutrons from the beam by absorbing layers has been shown to be due to the mechanisms of capture (with emission of a gamma-ray or of a heavy particle) and elastic scattering. Of these two mechanisms, the first seems to be predominately important in those elements exhibiting the largest cross sections for slow neutrons [2].
It seemed desirable to measure the absorption of neutrons in a single element, using a variety of detectors, and this letter is a preliminary report of investigations of that sort. Whi...
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Fra‐1 regulates vimentin during Ha‐RAS‐induced epithelial mesenchymal transition in human colon carcinoma cells
Intl Journal of Cancer, 2007
The process of epithelial mesenchymal transition, whereby cells acquire molecular alterations and fibroblastic features, is a fundamental process of embryogenesis and cancer invasion/metastasis. The mechanisms responsible for epithelial mesenchymal transition remain elusive. Human tumors frequently establish constitutively activated RAS signaling, which contributes to the malignant phenotype. In an effort to dissect distinct RAS isoform specific functions, we previously established human colon cell lines stably overexpressing activated Harvey-RAS (Ha-RAS) and Kirsten-RAS (Ki-RAS). Using these, we observed that only oncogenic Ha-RAS overexpression resulted in morphologic and molecular changes suggestive of epithelial to mesenchymal transition. We showed that ...
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Endothelin-1 induction of Glut1 transcription in 3T3-L1 adipocytes involves distinct PKCε- and p42/p44 MAPK-dependent pathways
Biochimica et Biophysica Acta (BBA) - General Subjects, 2008
We have shown previously that chronic exposure to endothelin-1 (ET-1) may stimulate GLUT1-mediated glucose transport in 3T3-L1 adipocytes via both protein kinase C (PKC)- and mitogen-activated protein kinase (p42/p44 MAPK)-dependent pathways. In the present study, by using a luciferase reporter driven by Glut1 promoter and enhancers (pLuc-GT1/E1/E2) and various constitutively active and dominant negative mutants of PKC isoforms, we identified PKCε as the PKC isoform involved. In addition, we provide evidence that there is no direct interaction between ET-1 activated PKCε and MAPK, at least at the kinase activity level. Furthermore, investigations employing deletion mutants of pLuc-GT1/E1/E2 to locate the putative ET-1 responsive sites and inhibitory agents...
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Poly-N-Acetyl Glucosamine Nanofibers Regulate Endothelial Cell Movement and Angiogenesis: Dependency on Integrin Activation of Ets1
J Vasc Res, 2007
Poly-N-acetyl glucosamine (pGlcNAc) nanofiber-derived materials effectively achieve hemostasis during surgical procedures. Treatment of cutaneous wounds with pGlcNAc in a diabetic mouse animal model causes marked increases in cell proliferation and angiogenesis. We sought to understand the effect of the pGlcNAc fibers on primary endothelial cells (EC) in culture and found that pGlcNAc induces EC motility. Cell motility induced by pGlcNAc fibers is blocked by antibodies directed against αVβ<sub>3</sub> and α<sub>5</sub>β<sub>1</sub> integrins, both known to play important roles in the regulation of EC motility, in vitroand in vivo. pGlcNAc treatment activates mitogen-activated protein kinase and increases Ets1, vascular...
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Shear Resistance of Pre-Cracking Reinforced Concrete T-Beam Strengthening with CFRP Sheets
AMM, 2013
Eleven pre-cracking reinforced concrete T-beams were strengthened with U-stirrups and longitudinal sheets of CFRP, the main parameters were the width, layer and space of U-stirrups of CFRP. The tests for shear resistance of the beams were carried out to measure the diagonal crack development and crack width, mid-span deflection, CFRP strain and ultimate resistance. Compared with the reference beam without strengthening, the diagonal cracks were effectively restrained and the shear resistances of the beams were also strengthened by the U-stirrups of CFRP. However, the shear resistance increment of the beams were not in direct proportion with the amount of CFRP sheet characterized by the width, layer and space of U-stirrups, as the strengthening effect is obvi...
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Investigations on the Δ23-, Δ24(28)- and Δ25-Sterols of zea mays
Phytochemistry, 1984
Abstract The sterols of Zea mays shoots were isolated and characterized by TLC, HPLC, GC/MS and 1 H NMR techniques. In all, 22 4-demethyl sterols were identified and they included trace amounts of the Δ 23 -, Δ 24 - and Δ 25 -sterols, 24-methylcholesta-5, E -23-dien-3β-ol, 24-methylcholesta-5, Z -23-dien-3β-ol, 24-methylcholesta-5,25-dien-3β-ol, 24-ethylcholesta-5,25-dien-3β-ol and 24-ethylcholesta-5,24-dien-3β-ol. In the 4,4-dimethyl sterol fraction, cycloartenol and 24-methylenecycloartanol were the major sterol components but small amounts of the Δ 23 -compound, cyclosadol, and the Δ 25 -compound, cyclolaudenol, were recognized. These various Δ 23 - and Δ 25 -sterols may have some importance in alternative biosynthetic routes to the major ster...
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New Insights into the Reactivity of Mn(II) Coordination Complexes with Dioxygen
Comments on Inorganic Chemistry, 1993
Abstract As a clearer picture of the oxygen activation processes by manganese(II) co-ordination complexes begins to emerge, we are more likely to understand the biological significance of the Mn-O2 interaction in various enzymes and to use the activated oxygen molecule for the catalytic oxidation of organic substrates. This Comment describes some of the recent advancements in the area of dioxygen activation by manganese(II) complexes. Details regarding the binding of the O2 moiety to the metal center and the reaction pathway that is taken during the four electron reduction of the O-O double bond are presented. Particular attention is given to new information on Mn(II) Schiff-base complexes as these results provide strong evidence for a more straightforward a...
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Role of compatibilizer in 3D printing of polymer blends
Additive Manufacturing, 2019
For additive manufacturing interfacial adhesion (bead-bead) remains an important issue affecting uniformity of mechanical properties. The present work examined the role a compatibilizer would play when used in fused filament fabrication (FFF) printing. Poly(styrene-maleic anhydride) (SMA) compatiblizer was mixed in varying compositions in blends of polyamide/poly(acrylonitrile-butadiene-styrene) (PA/ABS) to improve interfacial adhesion. Both small and large-scale 3-D component properties were examined. The mechanical property anisotropy ratio, an indication of bead-bead adhesive strength (defined as a property measured along the z axis versus the x axis) is representative of adhesive strength. Large-scale (big area additive manufacturing, BAAM) tests (flexur...
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Under Uniaxial Compression of Rock Specimens of Two Cracking of Shear Failure Mode Analysis
AMR, 2011
The dissertation introduced first simple uniaxial compression test of concrete, and in the specimen under uniaxial compression of the breakage mode reinforced and improved. For tensile destruction and shear failure damage model, bring forward the corresponding calculation model and some model set up compressive strength and tensile strength of the interrelations with each other, analysis of compressive strength and much higher than the tensile strength.In addition, from different model, drew diagrams with Matlab software，analysis of the compressive strength and tensile strength of the intrinsic value of relations.Finally, model for the primary split breakage and that shear failure can be ignored, used the buckling theory and the theory of mutation analysis...
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Soluble guanylate cyclase-α1is required for the cardioprotective effects of inhaled nitric oxide
American Journal of Physiology-Heart and Circulatory Physiology, 2011
Reperfusion injury limits the benefits of revascularization in the treatment of myocardial infarction (MI). Breathing nitric oxide (NO) reduces cardiac ischemia-reperfusion injury in animal models; however, the signaling pathways by which inhaled NO confers cardioprotection remain uncertain. The objective of this study was to learn whether inhaled NO reduces cardiac ischemia-reperfusion injury by activating the cGMP-generating enzyme, soluble guanylate cyclase (sGC), and to investigate whether bone marrow (BM)-derived cells participate in the sGC-mediated cardioprotective effects of inhaled NO. Wild-type (WT) mice and mice deficient in the sGC α 1 -subunit (sGCα 1 −/− mice) were subjected to cardiac ischemia for 1 h, followed by 24 h of reperfusion. Du...
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Pharmacogenetics of the Constitutive Androstane Receptor
Pharmacogenomics, 2007
The constitutive androstane receptor (CAR), a member of the NR1I group of nuclear hormone receptors, has been implicated in regulating the expression of genes that are critical in xenobiotic and endobiotic metabolism, uptake and elimination as well as genes involved in various other physiological processes. Hence, functional variation in CAR associated with its expression and/or activity can influence the transcriptional activation of its target genes and could contribute to the observed variation in drug response and toxicity. Moreover, coadministration of agents that are also CAR activators contributes to clinically relevant drug–drug interactions in patients receiving certain combination therapies. This review will discuss the functional significance of...
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[Cu81(PhS)46(tBuNH2)10(H)32]3+ Reveals the Coexistence of Large Planar Cores and Hemispherical Shells in High-Nuclearity Copper Nanoclusters
J. Am. Chem. Soc., 2020
Copper-based nanomaterials have attracted tremendous interest due to their unique properties in the fields of photoluminescence and catalysis. As a result, studies on the correlation between their molecular structure and their properties are of great importance. Copper nanoclusters are a new class of nanomaterials that can provide an atomic-level view of the crystal structure of copper nanoparticles. Herein, a high-nuclearity copper nanocluster with 81 copper atoms, formulated as [Cu81(PhS)46(tBuNH2)10(H)32]3+ (Cu81), was successfully synthesized and fully studied by X-ray crystallography, X-ray photoelectron spectroscopy, hydrogen evolution experiments, electrospray ionization mass spectrometry, nuclear magnetic resonance spectroscopy, and density functiona...
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Characteristics of graphite fluoride cathode prepared from residual carbon of graphite oxide.
NIPPON KAGAKU KAISHI, 1988
天然グラファイト (粒度 250～830μm および 61～74μm), 2800℃ で熱処理した石油コークス, 未処理石油コークスから酸化グラファイトを経て残留炭素をつくり, これを室温 ～600℃ でフッ素化してフッ化グラファイトを合成し, その組成, 構造および非水系リチウム電池の正極としての特性を調べた。400℃ で2時問減圧乾燥した残留炭素ほなお 12～25wt% の酸素を含み, 原料炭素より結晶性が低い。フッ素化反応速度は非常に速く, 低温かつ短時間でほとんどの場合 (CF)n が生成し, 得られた (CF)n も炭素材の直接フッ素化から合成される (CF)n より構造が乱れている。その正極特性は従...
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Dimeric FkpA Acts as an Anti-Aggregase on a Native Unfolded Membrane Protein Client
Biophysical Journal, 2019
Unfolded outer membrane proteins (uOMPs) transit through the aqueous periplasmic compartment of E. coli before reaching their native location in the bacterial outer membrane. The periplasm of E. coli contains a chaperone network that suppresses aggregation of nascent membrane protein polypeptides. FkpA, a heat shock periplasmic peptidyl-prolyl isomerase, functions as one of these chaperones. The periplasm does not contain external energy sources (i.e. ATP), which means that any chaperone activities of FkpA are accomplished using the free energy change available from complex formation. The crystal structure of FkpA is dimeric, which is thought to be the active form of the protein. To better understand the function of FkpA, we measured the equilibrium dissocia...
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On the incentives for security analysts to revise their earnings forecasts*
Contemporary Accting Res, 1990
Abstract. Several recent studies have empirically examined the accuracy of security analyst earnings forecasts. Among the objectives of this research is to measure the ability of analysts to gather and process both public and private information. An implicit assumption underlying these studies is that analysts' earnings forecasts fully reflect the information that the analysts possess. However, this may not be true; analysts may not incorporate all of their information into their forecasts. As shown here, analysts may be reluctant to revise their forecasts upon the receipt of new information because of the negative signal such a revision provides concerning the accuracy of their prior information. As a result, the accuracy of analysts' forecasts may underest...
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Effectiveness of five-element music therapy in cancer patients: A systematic review and meta-analysis
Complementary Therapies in Clinical Practice, 2021
To systematically evaluate the effectiveness of five-element music therapy on anxiety, depression, quality of life (QoL), sleep quality and Karnofsky performance score (KPS) in cancer patients. We searched English databases (PubMed, Cochrane Library, Embase, Web of Science) and Chinese databases (CNKI, WanFang, CBM and VIP database) from the inception to December 25, 2020. Two investigators independently screened literature, extracted data, and assessed risk of bias according to the eligibility criteria. The RevMan 5.3 software was used to perform the meta-analysis. A total of 22 studies, 2053 people with cancer were included. Meta-analysis showed that five-element music therapy had a significant difference for relieving depression (SMD = −1.11, 95% CI: 1....
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The effect of matrix cracking on mechanical properties in FRP laminates
Mech Adv Mater Mod Process, 2018
Abstract Background Fiber Reinforced Polymer (FRP) laminates have properties, which are highly dependent on the ply fiber orientations and which can be designed for optimum laminate performance. The purpose of this study is to investigate the effect of matrix cracking on the mechanical properties of FRP laminates with various off-axis angles, and to provide a critical test for an analytical solution using variational stress analysis. Methods Carbon and glass fiber reinforced polymer laminates (CFRP and GFRP) are tested. FRP prepregs are cured by using autoclave method to form laminates with layups [ θ m / φ n ] s . The laminates are then loaded monotonically and cyclically to obtain their mechanical properties and the effect of matrix cracks on the propert...
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A Novel Unsupervised Computational Method for Ventricular and Supraventricular Origin Beats Classification
Applied Sciences, 2021
Arrhythmias are the most common events tracked by a physician. The need for continuous monitoring of such events in the ECG has opened the opportunity for automatic detection. Intra- and inter-patient paradigms are the two approaches currently followed by the scientific community. The intra-patient approach seems to resolve the problem with a high classification percentage but requires a physician to label key samples. The inter-patient makes use of historic data of different patients to build a general classifier, but the inherent variability in the ECG’s signal among patients leads to lower classification percentages compared to the intra-patient approach. In this work, we propose a new unsupervised algorithm that adapts to every patient using the heart ...
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Detection of viruses directly from the fresh leaves of a Phalaenopsis orchid using a microfluidic system
Nanomedicine: Nanotechnology, Biology and Medicine, 2013
Early detection of pathogens is crucial for the effective surveillance of diseases. Many efforts have been made to explore methods which can detect these pathogens within a short period of time without requiring a tedious protocol. However, these developed methods have disadvantages such as they are relatively time-consuming or require specialized laboratory facilities. In this work, we have developed an integrated microfluidic system for rapid and automatic detection of viruses by direct analysis from fresh Phalaenopsis orchid leaves. The entire protocol, including ribonucleic acid (RNA) purification, reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring changes in turbidity was performed on a single chip....
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Synthesis and Antiretroviral Evaluation of New Alkoxy and Aryloxy Phosphate Derivatives of 3‘-Azido-3‘-deoxythymidine
J. Med. Chem., 1996
A series of new ether lipid-3'-azido-3'-deoxythymidine (AZT) conjugates (11a-g) were synthesized and evaluated for anti-HIV activity. The effect of chirality on the antiviral activity was examined through the synthesis of AZT conjugates bearing alkoxypropanols in the lipid portion of the molecule (11a-d). In addition, the long alkyl chain of alkoxyethyl ether lipid-AZT analogs was replaced with aromatic groups (11e-g), and the effect of this structural modification on activity is reported. The results of the biological tests indicate that analogs with a methyl group alpha to the phosphate moiety (11c,d) exhibit a marked degree of stereoselectivity with regard to their anti-HIV activity. Also, replacement of the long alkyl chain with aromatic groups in the ox...
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Antihypertensive efficacy and safety of Vaccinium arctostaphylos berry extract in overweight/obese hypertensive patients: A randomized, double-blind and placebo-controlled clinical trial
Complementary Therapies in Medicine, 2019
To evaluate the antihypertensive efficacy and safety of a standardized Vaccinium arctostaphylos (V. arctostaphylos) berry hydro-alcoholic extract in the overweight/obese hypertensive patients.Randomized placebo-controlled trial.Baqiyatallah hospital (Tehran, Iran).The antihypertensive efficacy and safety of 3-month intake of 400 mg extract capsule three times daily alongside standardized anti-hypertensive regimen in the treatment of 50 patients was compared with the placebo (n = 50).SBP (systolic blood pressure), DBP (diastolic blood pressure), body mass index, waist circumference, CBC (complete blood count), blood levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase), BUN (blood urea nitrogen) and creatinine....
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Droplet Microfluidics—A Tool for Single‐Cell Analysis
Angew Chem Int Ed, 2012
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse picoliter liquid capsules and manipulations at a throughput of thousands of droplets per second. These qualities allow many of the challenges in single-cell analysis to be overcome. Monodispersity enables quantitative control of solute concentrations, while encapsulation in droplets provides an isolated compartment for the single cell and its immediate environment. The high throughput allows the processing and analysis of the tens of thousands to millions of cells that must be analyzed to accurately describe a heterogeneous cell population so as to find rare cell types or access sufficient biological space to find hits in a directed evolution experiment. The low volumes o...
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Effect on Plasma Aldosterone, Renin Activity and Cortisol of Acute Volume Depletion Induced by Ethacrynic Acid under Constant Infusion of Angiotensin II and Dexamethasone in Man
Eur J Clin Investigation, 1976
Abstract. The influence on plasma aldosterone of acute volume depletion induced by ethacrynic acid was studied in man. The experiments were performed during the morning in supine healthy males receiving a control infusion of 5 % glucose or an infusion of angiotensin II (All) to suppress endogenous renin production or an infusion of dexamethasone to suppress endogenous ACTH. Ethacrynic acid induced in all circumstances a similar diuresis and volume depletion. The rise of plasma renin activity (PRA) was effectively suppressed by All and the rise of plasma Cortisol by dexamethasone. Plasma aldosterone (PA) rose markedly even when the elevation of PRA or Cortisol were suppressed. Yet when both endogenous renin and ACTH secretion were blocked, PA rose much less a...
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Molecular Cloning and Sequencing of the Circumsporozoite Protein Gene fromPlasmodium falciparumStrain FCC-1/HN and Expression of the Gene in Mycobacteria
J Clin Microbiol, 2001
Mycobacterium bovis bacillus Calmette-Guérin (BCG) has been used as a live bacterial vaccine to immunize more than 2 billion people against tuberculosis. In an attempt to use this vaccine strain as a vehicle for protective antigens, the Plasmodium falciparum gene from strain FCC-1/HN encoding circumsporozoite protein (CSP) was amplified from the P. falciparum genome, sequenced, and expressed in M. bovis BCG under the control of an expression cassette carrying the promoter of heat shock protein 70 (HSP70) from Mycobacterium tuberculosis. The recombinant shuttle plasmid pBCG/CSP was introduced into mycobacteria by electroporation, and the recombinant mycobacteria harboring pBCG/CSP could be induced by heating to express CSP; the molecular mass of recombinant ...
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Role of the cytomegalovirus major immediate early enhancer in acute infection and reactivation from latency
Med Microbiol Immunol, 2007
The cytomegalovirus (CMV) major immediate early (MIE) enhancer-containing promoter regulates the expression of the downstream MIE genes, which have critical roles in reactivation from latency and acute infection. The enhancer consists of binding sites for cellular transcription factors that are repeated multiple times. The primate and nonprimate CMV enhancers can substitute for one another. The enhancers are not functionally equivalent, but they do have overlapping activities. The CMV MIE enhancers are located between divergent promoters where the leftward genes are critical and essential for reactivation from latency and acute infection and the rightward gene is nonessential. The rightward transcription unit is controlled by an enhancer for murine CMV. In c...
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What does it take? Comparison of research standards for promotion in finance
Journal of Corporate Finance, 2018
Promotion decisions for professors are critical for any university and this is especially true when promotion also involves the granting of tenure. In this paper, we report the number of publications for Finance professors promoted to Associate or Full Professor at schools similar to the University of Georgia and also at the Top 10 Finance Departments. We also provide evidence on citations of the individuals' research. Our data reveal similarities in terms of the total number of articles published (between 6 and 8 for promotion to Associate Professor), the number of articles published in Finance “A” journals (about 3), and the number of citations between peer and aspirant schools. We find evidence that Associate Professors at Top 10 Departments have slig...
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Fretting wear of the nitrided medium carbon steel under line contact condition at an elevated temperature
ILT, 2019
Purpose Fretting wear exists widely in the field of matching mechanical parts whereas previous research studies mostly focus on the point contact through a ball-plate tribometer. This paper aims to study the influence of wear debris on the fretting wear characteristics of the nitrided medium carbon steel under line contact condition at elevated temperature. Design/methodology/approach Fretting wear behavior of the nitrided medium carbon steel was experimentally investigated under line contact condition at elevated temperature and different normal loads without lubrication. Wear loss, worn surface and wear debris were studied to analyze the wear mechanism of nitrided steel. Findings The results showed that surface hardness of the medium carbon steel was notab...
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A dietary score representing the overall relation of men’s diet with semen quality in relation to outcomes of infertility treatment with assisted reproduction
F&S Reports, 2021
To examine the impact of men's diet on outcomes of infertility treatment with assisted reproductive technology (ART) using an empirical score representing the relation of diet with semen quality.Prospective cohort study.Fertility center at an academic medical center.We included 296 men (688 semen samples) to identify an empirical dietary pattern and 231 couples (406 ART cycles) to investigate the association of this diet pattern with ART outcomes.Men's diet was assessed at baseline using a validated questionnaire. An empirical dietary pattern reflecting the overall relation of diet with semen quality was identified using reduced rank regression.The primary outcome was live birth per treatment cycle. The secondary outcomes were fertilization, implantation, an...
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Scrapie-associated prion protein accumulates in astrocytes during scrapie infection.
Proc. Natl. Acad. Sci. U.S.A., 1991
In the course of scrapie, a transmissible spongiform encephalopathy caused by an unconventional agent, a normal cellular protein is converted to an abnormal form that copurifies with infectivity and aggregates to form deposits of amyloid. We have used immunocytochemistry and methods that enhance detection of amyloidogenic proteins to investigate the types of cells in the central nervous system which are involved in the formation of the abnormal scrapie-associated protein. We show that this protein accumulates in astrocytes prior to the cardinal neuropathological changes in scrapie--astrogliosis, vacuolation, neuron loss, and amyloid deposition. These findings implicate the astrocyte in the formation of the scrapie isoform of the prion protein and amyloid in ...
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Social media use and parent–child relationship: A cross‐sectional study of adolescents
Journal Community Psychology, 2019
We examined the association between social media use and parent-child relationship quality and tested whether this association is independent of total screen time. Data on 9,732 students (48.4% female) aged 11-20 years were obtained from a provincially representative school-based survey. Heavy use of social media (daily use of more than 2 hr) was associated with greater odds of negative relationships between mother-daughter (odds ratio [OR] = 1.79; 95% confidence interval [CI]: 1.27-2.52), father-daughter (OR = 1.56; 95% CI: 1.16-2.09), father-son (OR = 2.19; 95% CI: 1.58-3.05) but not mother-son (OR = 1.17; 95% CI: 0.88-1.55). Results were similar after further adjusting for total screen time. There were no significant associations between regular use of so...
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Effect of liraglutide on body weight and pain in patients with overweight and knee osteoarthritis: protocol for a randomised, double-blind, placebo-controlled, parallel-group, single-centre trial
BMJ Open, 2019
Introduction With an increasing prevalence of citizens of older age and with overweight, the health issues related to knee osteoarthritis (OA) will intensify. Weight loss is considered a primary management strategy in patients with concomitant overweight and knee OA. However, there are no widely available and feasible methods to sustain weight loss in patients with overweight and knee OA. The present protocol describes a randomised controlled trial evaluating the efficacy and safety of the glucagon-like peptide-1 receptor agonist liraglutide in a 3 mg/day dosing in patients with overweight and knee OA. Methods and analysis 150 volunteer adult patients with overweight or obesity and knee OA will participate in a randomised, double-blind, placebo-controlled, p...
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Cracking and Delamination Behavior of Gold Thin Strip Deposited on Polycarbonate Plate under Cyclic and Stepwisely-Increased Trapezoidal Tension
AMM, 2014
Cracking and delamination behavior of small rounded-rectangular gold thin films called “strips” with a thickness less than one micrometer was observed under cyclic and stepwisely-increased trapezoidal tensions. Three strips in different directions were deposited on a polycarbonate plate specimen which was subjected to tensile loading. Straight slit and Vickers indentation were introduced as initial defects on each strip and cracking and delamination which were initiated around the defects were observed. In strips with slit, delamination was observed when introducing the slit. It was suggested that the delamination was caused by the residual stress accumulated during deposition. The growth of delamination was also observed during cyclic tension. In strips...
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Implications of Pulsed Chemical Exposures for Aquatic Life Criteria and Wastewater Permit Limits
Environ. Sci. Technol., 2006
Subacute effects of pulsed copper, zinc, or ammonia exposures were examined, including a range of pulse concentrations, durations, frequencies, and recovery times between pulses, using short-term chronic Pimephales promelas and 21-d Daphnia magna tests. Sublethal effects were rarely observed independent of mortality. Effects were observed only at concentrations near the species continuous exposure 48 h LC50 for each chemical. Daphnia often rebounded from temporary reproduction effects, meeting or exceeding control responses by the end of the test. Effects of 24 h ammonia or copper pulses were diminished soon after the pulse was removed, while 24 h zinc pulses caused continued effects for several days following removal of the pulse, indicating a slower uptake...
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Plasticizer-Free Thin-Film Sodium-Selective Optodes Inkjet-Printed on Transparent Plastic for Sweat Analysis
ACS Appl. Mater. Interfaces, 2020
A novel strategy to functionalize transparent flexible plastic films with an optical ion-sensing layer using an inkjet-printing technology is described. The hydrophobic sensing chemicals that include a sodium ionophore, a lipophilic proton chromoionophore, and a lipophilic ion-exchanger are co-deposited onto substrates such as transparent polyester film sheets in the absence of any plasticizer and/or hydrophobic polymer matrix. The inkjet-printing process enables the formation of optode films with nanoscale thickness/roughness that readily facilitate interfacing with aqueous samples. Using a smartphone detector, the colorimetric response of the optodes is shown to reach 95% of equilibrium values within 100 s in response to different concentrations of sodium ...
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Longitudinal trajectory of Amyloid‐related hippocampal subfield atrophy in nondemented elderly
Human Brain Mapping, 2020
Hippocampal atrophy and abnormal β-Amyloid (Aβ) deposition are established markers of Alzheimer's disease (AD). Nonetheless, longitudinal trajectory of Aβ-associated hippocampal subfield atrophy prior to dementia remains unclear. We hypothesized that elevated Aβ correlated with longitudinal subfield atrophy selectively in no cognitive impairment (NCI), spreading to other subfields in mild cognitive impairment (MCI). We analyzed data from two independent longitudinal cohorts of nondemented elderly, including global PET-Aβ in AD-vulnerable cortical regions and longitudinal subfield volumes quantified with a novel auto-segmentation method (FreeSurfer v.6.0). Moreover, we investigated associations of Aβ-related progressive subfield atrophy with memory decl...
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Phenotypic Analysis of Dlx5 Overexpression in Post-natal Bone
J Dent Res, 2008
Dlx5 plays an important role in the embryonic development of mineralized tissues. We hypothesized that Dlx5 also functions in regulating post-natal bone formation in mice. To prove this hypothesis, we infected 5-day-old bone sialoprotein (BSP)/avian retroviral receptor gene (TVA) transgenic mice with replication-competent retroviral vectors expressing wild-type Dlx5 (RCAS- Dlx5WT) and mutated Dlx5 at arginine (R) 31 of its homeodomain (RCAS- Dlx5RH). Immunohistochemistry indicated that RCAS- Dlx5WT increased BSP and osteopontin (OPN) expression, whereas it decreased that of osteocalcin (OC). RCAS- Dlx5RH mediated opposite effects. Semi-quantitative RT-PCR confirmed these results. Ex vivo overexpression of RCAS- Dlx5WT in BSP/TVA calvarial cells promoted, whe...