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Pus and Wound

This document provides guidelines for collecting and processing pus samples for culture and sensitivity testing. It describes collecting pus samples using sterile technique and transporting them in appropriate media. Samples are plated on blood, chocolate and MacConkey agars and inoculated in thioglycollate broth for culturing aerobic and anaerobic pathogens. Gram stains are performed and isolates are identified and tested for antibiotic sensitivity. Results including Gram stains are reported within hours or days depending on growth.

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Armilyn Garcia Navarro
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189 views16 pages

Pus and Wound

This document provides guidelines for collecting and processing pus samples for culture and sensitivity testing. It describes collecting pus samples using sterile technique and transporting them in appropriate media. Samples are plated on blood, chocolate and MacConkey agars and inoculated in thioglycollate broth for culturing aerobic and anaerobic pathogens. Gram stains are performed and isolates are identified and tested for antibiotic sensitivity. Results including Gram stains are reported within hours or days depending on growth.

Uploaded by

Armilyn Garcia Navarro
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPS, PDF, TXT or read online on Scribd
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Pus (wound, Abscesses, Burns and

sinuses) culture & sensitivity


Aim of the test
 Isolate and identify aerobic and
anaerobic pathogenic organisms pus
specimen.
 Types of specimen:
 Swabs from the infected area or
aspiration from deep wounds. Swabs in
anaerobic transport media for the
isolation of anaerobes.
Criteria of specimen rejection
 Inappropriate specimen transport
device; mislabeled specimen;
unlabelled specimen.
 dried samples and specimen received
after prolonged delay (usually more
than 72 hours).
 specimen received in expired
transport media
Pathogen and commensals
Specimen collection
 Pus from abscess is best to collected at the time, the
abscess is incised and drained.
 Using sterile technique, aspirate or collect from
drainage tube up to 5 ml of pus, transfer to sterile
container.
 If pus is not being discharged use sterile cotton wool
swab to sample from the infected site.
 extend the swab deeply into the depth of the lesion.
 Immerse the swab in container of transport medium
 label it and send to the laboratory as soon as possible
Quantity of specimen

 Sufficient amount on swab, or


aspiration in transport media or
syring
 Time relapse before processing the
sample: 30min.
 Storage
 Maintain specimen swab at room
temperature. Do not refrigerate.
Specimen processing
 Media
 ‰Blood Agar,
 ‰Chocolate Agar,
 ‰MacConkey Agar
 ‰Thioglycollate broth
Culturing procedure

 Streak one blood agar plates, one


chocolate, MacConkey and inoculate
thioglycollate broth tube.
 Gram stain to check the presence or
absence and if present the type or
types and the predominant
organisms.
Post specimen processing
 Interfering factors:
 Patient on antibiotic therapy.
 Improper sample collection.
 Result reporting:
 Report Gram stain finding as an initial
report.
 Report the isolated pathogen/s and its
sensitivity pattern as a final report.
 Turn around time:
 Gram stain results should be available 1
hour after specimen receipt.
 Isolation of a possible pathogen can be
expected after 2-3 days.
 Negative culture will be reported out 1-2
days after the receipt of the specimen.
Additional information

 Contamination of the specimen with normal


flora is one of the major obstacles in
obtaining good results.
 Care should be taken to avoid
contaminating the specimen with normal
flora.
 This could e accomplished by swabbing
superficial infected wounds with 70%
alcohol.

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