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This document provides instrument settings and parameters for performing a protein quantification assay using the biuret method and spectrophotometry. Key details include using a wavelength of 540 nm, a sample volume of 4 μL, and reagent volumes of 200 μL. The dynamic range is 0 to 150 g/L protein concentration. A two-point linear calibration is specified using assigned standard values and factors to calculate concentration from absorbance readings.

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Enrique Duarte
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69 views1 page

Prot

This document provides instrument settings and parameters for performing a protein quantification assay using the biuret method and spectrophotometry. Key details include using a wavelength of 540 nm, a sample volume of 4 μL, and reagent volumes of 200 μL. The dynamic range is 0 to 150 g/L protein concentration. A two-point linear calibration is specified using assigned standard values and factors to calculate concentration from absorbance readings.

Uploaded by

Enrique Duarte
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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PROTEIN

Spectrophotometric
BIURET

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: AU 400 / 640


Reagent preparation
Reagent is ready to be used.

Instrument settings

Specific Test Parameters


Sample: Volume 4 µL Dilution 0 µL
Reagents: R1 Volume 200 µL Dilution 0 µL Reagent OD limit:
Wavelength: Pri. 540 Sec. First L -0.5 First H 0.1
Method: END Last L -0.5 Last H 0.1
Reaction slope: + Dynamic Range:
Measuring Point 1: First 0 Last 27 L 0 H 150
Measuring Point 2: First 0 Last 0 Correlation Factor:
Linearity: % A 1 B 0
No Lag Time: On-board stability period:

L H
7. None selected
8. Out of range 65 80
L H
Panic Value Unit: g/L Decimal places: 0

Calibration Specific

Calibration Type: AB Formula: Y=AX+B Counts: 2 Process: CONC.

Cal. Nº OD CONC Factor/OD-L Factor/OD-H


Point 1: # * -9999 9999

* Enter the assigned standard value


# User defined

Vers. 0206

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