Abacus 5 tubing guide

This guide was created to help service engineers to repair or refurbish five part analyzers by changing
used or dirty tubing of the unit to a new set. Tubing is an important and sensitive part of the
analyzer. The length, diameter and material of the tubing are very important to ensure the correct
function of the unit. It is highly recommended to use the defined tube types and solutions given in
this guide. Non-compliance in the use of the recommended tubing system could create overflow and
damage electical parts of the unit.

The shear valve tubing is not included in this guide.

Content of the guide:

- Total tube system on the valve block (tube types with length and part number)

- Preparing of "T" type tubes

- Step by step process how to connect the tubes

- Graphics to show how the tubing should be installed

Tools needed:

- Dry cloth or towel

- Cutter

- Ruler or tape measure

Note: Sections of the tubing system can be also changed by using this guide

Warning
The original tubing system may contain human sample, clot and infective particles. Before removing
any tubing, it is highly recommended to prime cleaner solution through the unit by connecting
cleaner to all the inlets. Make stress or blank measurements to remove these particles to make
removal of the tubing more safe.

When re-tubing an analyzer always do this with completely drained system. Preparing for shipment is
highly recommended before removing the tubing (after cleaning is ready).

The removed tubing parts are highly biohazard material so place all removed tubing and parts into a
Biohazardous material container.
Preparing of the "T" tubes:

The system contains tubes connected to a plastic junction. These tubes are called "T" tubes and they
are pre-defined to make the tubing exchange faster. Creating these tubes is necessary before starting
the exchange of the tubing. The "T" tubes are indicated with letter T and the identification number.

Junctions in the tubing system:

small "T" junction part number (A5451) Not needed for valve block tubing

middle "T" junction part number (A556) in T0, T1, T2, T3, T5, T7, T12, T16

large "T" junction part number (A545) in T4, T6, T9, T10, T11,

"Y" junction part number (A546) in T2, T3, T8, T13, T14, T15, T16

During the procedure, the tube data is indicated between () signs. (order number, external/internal
diameter, tube material) Please use the images and the steps in combination.

List of „T” tubes:

T0
T0 T1

4 8 Glass
V 12/2 V 15/1 7 12
Reservoir V2/2
bottom
10
2

V 1/3 V 12/3

3,5 3,5 V13/2 V12/1

T2
2
2 2 2 2,5 2,5
3,5
V4/3 V6/3 V8/3
25 6
10
P
3,5 3,5 3,5 2
3
2 2 2
V3/3 V5/3 V7/3 V23/2
T3 V10/1

8 4 22 Vacuum
Chamber
2,5
2 2
16

V15/2 V9/1 V11/1

T4 T5 (2 inlet wash head) V13/1

2 7 2
WBC Shear 2
chamber Valve
40 2 119 4
Wash head V14/1

V29/1 V11/2

T6
T5 (3 inlet wash head) V13/1

22 4
2 V36/2 V17/3

114 4 3
V14/1

V26/2

T7 T8

V20/2 Dilu Reservoir

1. 9

4 31
V16/1
47 Sheath
Reservoir Dilu Reservoir 6
2.
1,7

V32/2

T9
Dilu Dilu
Reservoir 2. Reservoir 1.

2 2
2,5 18,5 8 20 3,5 9,3
V43/1 V36/1

3,5 2 2

2,5
V41/1 V42/1 V40/1

10

V37/1
T10 T11
MIX RBC
chamber chamber

2 3,5

40 40
V42/2 V29/2

9 9

V9/2 V10/2

T13 (Glass reservoir with 3 inlet)

T12
V34/2 2,5
Glass Reservoir
top 20
2 V3/2

Glass Reservoir 7
Vacuum 6 3
V35/2 middle
Reservoir

T14 T15

Dilu Reservoir Vacuum

4
1. Reservoir 10
20
V8/2 23
V23/1

Dilu Reservoir 4 Vacuum 10

2. Reservoir

T16 (For V10/1

SSM only)
8
4 3 22 Vacuum
chamber
2,5
2 2 60
16

V9/1 V11/1 V22/2

V15/2
Step by step guide:

Step1.1:

Locate the hole on the bottom right side of the valve door. Pass the two tubes (A542, 6/3, silicon)
connected to the pumps through the hole. Both tubes should have the converter (A547, 3.2/3.2) at
the end as shown on the image below. 20 cm tube is connected to pump 1's (outer pump) top, 50 cm
tube is connected to pump 2's (internal pump) top.

Step1.2:

Connect the 35 cm tube (A543, 4/1.8, tygon) to valve 13/3 and connect the other end to the
converter (A547, 3.2/3.2) on pump tube 50 cm.

Step1.3:

Connect the other 35 cm tube (A543, 4/1.8, tygon) to valve 14/3 and connect the other end to the
converter (A547, 3.2/3.2) on pump tube 20 cm.

Step1.4:

Connect the 56 cm tube (A5401, 4/2, RCT-NOVA-971) to valve Diff 5 P puffer reservoir's bottom front
inlet. Thread the other end through the hole (to pumps), under the pumps through the tube holders
and to the Diff 5 P metal inlet.

Step1.5:

Connect the 73 cm tube (A543, 4/1.8, tygon) with green marker to valve 16/3. Thread the other end
through the hole (to pumps), under the pumps through the tube holders and to Diluent metal inlet.

Step1.6:

Attach tube "T0" to valve 1/3 (10 cm) and valve 12/2 (4 cm). Thread the unattached end through the
hole (To glass reservoir's bottom) and attach it to the bottom inlet of the glass reservoir.

Step1.7:

Connect the other 52 cm tube (A543, 4/1.8, tygon) with yellow marker to Lyse reservoir's bottom
front inlet. Thread the other end of the tube through the hole (to pumps) under the pumps through
the tube holders and to the Lyse metal inlet.
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's
TOP

T0

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM
73
T0 T0 35
35

C
C
C
Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 EmptyC Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

20
Vacuum Sheath Diff 5 P Diluent Diluent
Lyse
reservoir reservoir reservoir reservoir reservoir 50
reservoir
1 2
C
To pumps
CC C

52

56
C
C C
C
Step2.1:

Connect a 22 cm tube to valve 3/2 and lead the other end through the hole (to glass reservoir's top)
and attach it to the glass reservoir's top inlet. Note that some glass reservoirs has two inlets on the
top. In this case "T13" should have a "Y" junction added with 2,5 cm and 7 cm on the two ends, so
both glass reservoir’s inlets can be covered with tygon. See the image below.

Step2.2:

If washing head has two inlets:

Connect the "T" fitting middle of tube "T5" to valve 13/1 (2 cm) and 14/1 (4 cm). Pass the other end
of the tube (119 cm with the red mark) above the reservoirs, under the dilutors to the XY unit and
attach it to the washing head's top inlet. Use the tube holders attached to the frame to grip the tube.

If washing head has 3 inlets:

The long tube of "T5" is 114 cm. Add an "Y" junction to this tube with 5 cm tube on both other ends.
Connect these 5 cm tubes to the top and bottom inlet of the washing head.

Step2.3:

Connect the 108 cm tube (A543, 4/1.8, tygon) to valve 18/2 and pass the other end of the tube above
the reservoirs, under the dilutors to the XY unit and attach it to the washing head's bottom inlet.

If washing head has 3 inlets:

Connect the same tube to the middle inlet of the washing head.

Step2.4:

Connect the 25 cm tube (A543, 4/1.8, tygon) to valve 24/1. The other end of the tube should have a
converter (2.3/1.6). This end will be connected to the shear valve with 30 cm tube (A5433, 2.23/1.27,
tygon).

Step 2.5:

Connect valve 17/1 and valve 18/3 with an 5 cm tube (A543, 4/1.8, tygon).

Step 2.6:

Connect valve 14/2 and valve 15/3 with a 4 cm tube (A543, 4/1.8, tygon).

Step 2.7:

Connect valve 43/2 and valve 44/3 with a 4 cm tube (A543, 4/1.8, tygon).
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's T13
TOP

T0

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM
5 73 108
4
T0 T0 T5 35
25 35

C
C
C
Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 C
Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

T5

20
Vacuum Sheath Diff 5 P Diluent Diluent
Lyse
reservoir reservoir reservoir reservoir reservoir 50
reservoir
1 2 CC
To pumps
C
C
52

56
CC
C
C
Washing
head
Step 3.1:

Connect valve 24/3 to valve 25/1 with a 5 cm tube (A543, 4/1.8, tygon).

Step 3.2:

Connect valve 25/3 to valve 26/1 with an 5 cm tube (A543, 4/1.8, tygon).

Step 3.3:

Connect valve 1/2 to the tube transition 7th pin with a 33 cm tube (A543, 4/1.8, tygon).

Step3.4:

Connect valve 1/1 to valve 19/2 with a 25 cm tube (A543, 4/1.8, tygon).

Step 3.5:

Connect "T1" tube's 2 cm long tube to valve 12/3, the two "side" tubes are going to valve 15/1 (7 cm)
and valve 2/2 (12 cm) as shown on the image below.

Step 3.6:

Connect the 1,5 cm tube (A543, 4/1.8, tygon) with exausting went to valve 5/2.
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's T13
TOP

T1
33
25

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11

Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM
4 5 108
T1
5 5
25

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

Vacuum Sheath Diluent Diluent

Diff 5 P Lyse
reservoir reservoir reservoir reservoir
reservoir reservoir
1 2

To pumps

Washing
head
Step 4.1:

Connect the Diff 5P reservoir's top inlet to valve 6/2 with a 25 cm tube (A5401, 4/2, RCT-NOVA-971).

Step 4.2:

Connect "T9" tube as shown on the image.

Connect the 9.3 cm end of the "T9" tube to valve 36/1. Follow the tube to the nearest "T" junction
with 2 cm tube and connect it to valve 40/1. Connect the next "T" junction with 2 cm tube to valve
42/1.

Connect the 10 cm end of "T9" and connect it to valve 37/1. Follow the tube to nearest "T" junction
with 2.5 cm tube and connect it to valve 41/1. Connect the next "T" junction with 2.5 cm tube to
valve 43/1.

Connect the last two "T" fitting with 2 cm tubes to Diluent reservoir's bottom rear inlets. Settle the
tube as shown on the image below.

Step 4.3:

Connect "T14" "Y" junction with the 4 cm tubes to the top inlets of the two Diluent reservoirs.
Connect other end of "T14" to valve 8/2 (23 cm) as shown on the image.

Step 4.4:

Connect lyse reservoir's top inlet to valve 7/2 with a 25 cm tube (A543, 4/1.8, tygon).

Step 4.5:

Connect "T8" "Y" junction with 6 cm tube to Diluent reservoir 2 bottom front inlet, connect the 9 cm
tube to Diluent reservoir 1 bottom front inlet. Connect the 31 cm tube of "T8" to valve 16/1 as shown
on the image.

Step 4.6:

Connect "T4" "T" fitting (with 2 cm tube facing up) to bottom of WBC chamber. Find the second "T"
fitting (with 2 cm tube facing down) and connect it to valve 11/2. Connect the 40 cm tube to valve
29/1 with a curve shown on the image below.
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's
TOP

T1 25
33 T4
25 25
T14

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11

Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM
T1
5 5 T8
T4

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

T9
T14

Vacuum Sheath Diff 5 P Diluent Diluent

Lyse
reservoir reservoir reservoir reservoir reservoir
reservoir
1 2
To pumps

T8

T9
Step 5.1:

Find "T11" "T" junction and connect the 2 cm long tube to RBC chamber's bottom. Connect the other,
9 cm long tube on "T" junction to valve 10/2. Lead the 40 cm long tube of "T11" as shown on the
image and connect it to valve 29/2.

Step 5.2:

Find "T10" "T" junction and connect the 2 cm long tube to MIX chamber's bottom. Connect the other,
9 cm long tube on "T" junction to valve 9/2. Lead the 40 cm long tube of "T10" as shown on the
image and connect it to valve 42/2.

Step 5.3:

Connect a 6 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 2/1.

Step 5.4:

Connect "T7" "T" fitting with 4 cm tube to valve 20/2 and 1.7 cm long tube to valve 32/2. Connect the
middle, 47 cm long tube of the "T" fitting to the bottom of sheath reservoir's rear inlet as shown on
the image.

Step 5.5:

Connect a 12 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 18/1.

Step 5.6:

Connect a 6 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 19/1.
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's
TOP

T11 25
T4

6 25 T10
T14

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM 6
12
T8
T11 T4
T7

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

T10

T9
T14

Vacuum Sheath Diff 5 P Diluent Diluent

Lyse
reservoir reservoir reservoir reservoir reservoir
reservoir
1 2

To pumps

T8
T7

T9
Step 6.1:

Connect the 4 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 20/1.

Step 6.2:

Connect valve 38/1 to the bottom of the Diff 5P reservoir's rear inlet with a 40 cm tube (A5401, 4/2,
RCT-NOVA-971) as shown on the image below.

Step 6.3:

Connect "T6" "T" junction with 4 cm tube to valve 17/3 and the 3 cm tube to valve 26/2. Connect the
22 cm long tube of "T6" valve 36/2.

Step 6.4:

Connect valve 39/1 to the bottom of the Lyse reservoir's rear inlet with a 34 cm long tube (A543,
4/1.8, tygon).

Step 6.5:

Connect "T12" "T" junction with the 3 cm tube to valve 35/2 and the 2 cm tube on the middle of the
"T" fitting to valve 34/2. Connect the 6 cm tube to the top of the Vacuum reservoir's front inlet.

Step 6.6:

Connect a 5 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 28/1 .
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's
TOP

T11

6 T10

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM 6
12
T6
5
T11 4
T7

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

T10

T12
34

Vacuum Sheath Diluent Diluent

Diff 5 P Lyse
reservoir reservoir reservoir reservoir
reservoir reservoir
1 2

To pumps

T7
40
Step 7.1:

Connect "T2" "Y" junction with 10 cm tube to pressure sensor board's sheath sensor (right side). Find
the 3 cm tube to the first "T" junction with 2 cm tube in the middle and connect the tube to valve
3/1. Find the next "T" junction with 2 cm tube in the middle and connect the tube to valve 5/1. Find
the 3rd "T" junction with 2 cm tube in the middle and connect the tube to valve 7/1.

Connect the 2cm tubes on the "T" junctions on the opposite side of "T2" to valve 4/2, valve 6/1 and
valve 8/1.

Find the "Y" junction connecting the two sides of "T2". Lead the 25 cm tube from "Y" junction under
valve 13.

Connect the first "T" junction's middle 2,5 cm tube to valve 13/2. Find the last "T" fitting of "T2" and
connect the 2,5 cm long tube on the junction to valve 12/1 and the 2 cm tube to 23/2. See the image
below.

Step 7.2:

Find "T3" "Y" junction and connect the 16 cm tube on the middle of the junction to 15/2. Find the 8
cm tube on the other side of the "Y" junction and connect it to valve 10/1.

Follow the 2,5 cm tube to "T" fitting, find the 2 cm tube on the middle of the junction and connect it
to valve 9/1. Find the second "T" fitting with the 2 cm tube on the middle and connect it to valve
11/1.

Find the 22 cm tube on the "T" fitting and connect it to the bottom of the Vacuum chamber.

Step 7.3:

Connect a 5 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 30/1.

Step 7.4:

Connect a 8 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 33/1.

Step 7.5:

Connect a 3 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 33/2.

Step 7.6:

Connect a 11 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 44/1. Set the tube as
shown on the image below.
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's
TOP

T2
T3

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM 11 3

T2 T6
5
4 8
5

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

T12
34

Vacuum Sheath Diff 5 P Diluent Diluent

Lyse
reservoir reservoir reservoir reservoir reservoir
reservoir
1 2

To pumps

40
Step 8.1:

Connect the Drain sensor's right side on the middle of the pressure sensor board to the top inlet of
the Vacuum chamber with a 12 cm tube (A543, 4/1.8, tygon).

Step 8.2:

Connect the Chamber sensor's right side on the top of the pressure sensor board to the top rear inlet
of the Vacuum reservoir with a 33 cm tube (A543, 4/1.8, tygon).

Step 8.3:

Connect valve 17/2 to the RBC chamber's lower inlet on the side of the chamber with a 19 cm tube
(A543, 4/1.8, tygon).

Step 8.4:

Connect valve 30/2 to the WBC chamber's lower inlet on the side of the chamber with a 19 cm tube
(A543, 4/1.8, tygon).

Step 8.5:

Connect valve 4/3 to Sheath reservoir's top inlet with a 28 cm tube (A543, 4/1.8, tygon).

Step 8.6:

Connect valve 16/2 to the bottom of Sheath reservoir's front inlet with a 40 cm tube (A543, 4/1.8,
tygon).
 Pressure sensor Vacuum MIX RBC WBC
board 12 chamber chamber chamber chamber

To glass
reservoir's
TOP

T2
19 T3

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM 11 3

19
T2
33 8
5

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

28

40

Vacuum Sheath Diff 5 P Diluent Diluent

Lyse
reservoir reservoir reservoir reservoir reservoir
reservoir
1 2

To pumps
Step 9.1:

Connect valve 34/1 to RBC chamber's top inlet on the side of the chamber with a 36 cm tube (A543,
4/1.8, tygon).

Step 9.2:

Connect valve 35/1 to WBC chamber's top inlet on the side of the chamber with a 42 cm tube (A543,
4/1.8, tygon).

Step 9.3:

Connect valve 37/2 to valve 26/3 with a 14 cm tube (A543, 4/1.8, tygon) as shown on the image
below.

Step 9.4:

Connect valve 39/2 to valve 28/3 with a 14 cm tube (A543, 4/1.8, tygon) as shown on the image
below.

Step 9.5:

Connect a 17 cm tube (A5401, 4/2, RCT-NOVA-971) with 1.6/2.3 converter to valve 38/2. Set the tube
as shown on the image below. (The converter will be connected to the tube transition 4th pin with 20
cm tube (A5433, 2.23/1.27, tygon).

Step 9.6:

Connect valve 44/2 to valve 33/3 with a 14 cm tube (A543, 4/1.8, tygon) as shown on the image
below.
 Pressure sensor Vacuum MIX RBC WBC
board 12 chamber chamber chamber chamber

To glass
resreservoir's
TOP

36 19
42

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM

17 19
20
33

Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

14 14 14

28

40

Vacuum Sheath Diluent Diluent

Diff 5 P Lyse
reservoir reservoir reservoir reservoir
reservoir reservoir
1 2

To pumps
Step 10.1:

Connect a 15 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 24/2.

Step 10.2:

Connect a 12 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 25/2.

Step 10.3:

Connect valve 41/2 to valve 30/3 with a 14 cm tube (A543, 4/1.8, tygon) as shown on the image
below.

Step 10.4:

Connect valve 40/2 to valve 29/3 with a 14 cm tube (A543, 4/1.8, tygon) as shown on the image
below.

Step 10.5:

Connect a 5 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 28/2. The converter will be
connected to the tube transition 5th pin with 20 cm tube (A5433, 2.23/1.27, tygon).

Step 10.6:

Connect "T15" 23 cm long tube (single end) to valve 23/1. Connect both 10 cm tubes on the other
side of the "Y" junction to the vacuum reservoir's bottom inlets.

Step 10.7:

Connect a 2 cm tube (A543, 4/1.8, tygon) with 1.6/2.3 converter to valve 32/1. The converter will be
connected to the tube transition 6th pin with 14 cm tube (A5433, 2.23/1.27, tygon).
 Pressure sensor Vacuum MIX RBC WBC
board chamber chamber chamber chamber

To glass
reservoir's
TOP

14

36
42

Valve1 Valve2 Valve3 Valve4 Valve5 Valve6 Valve7 Valve8 Valve9 Valve10 Valve11
Valve12 Valve13 Valve14 Valve15 Valve16 Valve17 Valve18 Valve19 Valve20 Empty Empty

To glass
reservoir's
BOTTOM
20
5
17 20
15 12 2

14
Valve23 Valve24 Valve25 Valve26 Empty Valve28 Valve29 Valve30 Empty Valve32 Valve33

Valve34 Valve35 Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43 Valve44

14 14 14 14

T15

Vacuum Sheath Diff 5 P Diluent Diluent

Lyse
reservoir reservoir reservoir reservoir reservoir
reservoir
1 2

To pumps
 Step 11:

Connect dilutor pistons with the appropriate valves according to the image. Use same tube (A543,
4/1.8, tygon) as in the valves. Valve 38 to dilutor piston is a different type (A5401, 4/2, RCT-NOVA-
971).

Valve36 Valve37 Valve38 Valve39 Valve40 Valve41 Valve42 Valve43

37

37

37

37

35

35

35

17

Dilutor block 1 (left) Dilutor block 2 (right)

Tubing on valve block is done.