PROTOZOANS
Flagellates
                                                           • Axoneme: root/intracellular part of the flagellum
                                                           • Axostyle: sheet of microtubules
A. Phylum Sarcomastigophora
                                                           Rodlike structure that fxns in cellular support
Sexual reproduction
                                                           • Kinetosome: granule to which the flagellum
• Subphylum Sarcodina: Amoeba
                                                           • Cytosome: cell mouth
 Locomotory: pseudopodia (false foot) - Habitat:
                                                           • Cytopyge: cell anus
intestinal tract (majority), CNS
•    Subphylum Mastigophora:           Flagellates         -   All are commensal except G. lamblia, D,
Locomotory: flagella - Habitat: intestinal lumen,              fragilis, and T. vaginalis
mouth, vagina, blood                                       - All are inhabitants of large intestine/spleen
                                                               except G. lamblia & Trichomonas spp.
B. Phylum Ciliophora: Ciliates                             - Only Trichomonas has tropho as infective stage
Distinct cytosome (mouth) structure Dimorphic              Classifications:
nuclei - macronucleus and micronucleus
Large intestine - Balantidium coli                         1. Intestinal Flagellates
                                                           **Giardia lamblia – G. intestinalis / G.
C. Phylum Apicomplexa                                      duodenalis
Usually No Cilia and Flagella - Special structure:                                  Cyst
apical complex for penetrating host cells                  Patho: Giardiasis/lambliasis, diarrhea, dehydration
Plasmodium, Babesia, Toxoplasma, etc.                      abdominal pain, steatorrhea (fats greater than 5g
                                                           sa stool), traveler’s diarrhea
                  AMOEBA                                                       Trophozoite:
Pseudopodia – footlike projection                          “old man’s eyeglasses” tear drop shape, 2
Karyosome- Are of chromatin w/i nucleus                    axonemes; Axostyle-midrib structure for support
Cyst – non-motile, infective s., sa formed stool           **Chilomastix mesnili – American Lemon cyst
Trophozoite – vegetative/feeding stage, bla                Entromonas hominis, Retortamonas intestinalis
                                                           Dientamoeba fragilis
Classifications                                             2. Urogenital Flagellates
    1. Intestinal: Pathogenic                              Trichomonas vaginalis, T. tenax (mouth)
**E. histolytica – naka /E. dispar pag reporting                       Ciliates – Balantidium coli
Often with charcott-leyden crystals, mostly                Cilia, macronucleus – bean shape, micronucleus
asymptotic, similar din kay E. moshkovskii                 – small nucleus. IH – pigs; at risk: hog raisers,
                  Non-pathogenic                           treatment: metronidazole
**E. coli – Dirty cytoplasm (table)                                       Blastocystis Hominis
**Endolimax nana – CN – “cross eyed cyst”,                 -previously classifies as yeast, usually asymptotic
smallest amoeba, peripherally nucleated                    cyst forms: vacuolated/granular/amoeboid form
**Iodamoeba butschlii – CN – “iodine cyst”                 MOT – ingestions, habitat: small intestine
(cyst)Rarely binucleated -> nucleus at periphery,
amoeba of pigs                                             Stool exam (ova and parasite exam)
E. gingivalis - no cyst stage – no encystation             1. Direct fecal smear
lives on the surface of gum and teeth not sa large         2. Concentration techniques - Formalin Ether
intestine, gotten through kissing, sharing                 Concentration Technique (FECT)
utensils, not through ingestion/fecal-oral route           3. Permanent stained smears
E. dispar, E. polecki                                      - Iron hematoxylin
    2. Free-living (pweds CNS) –                           - Trichrome stain
         Acanthamoeba and Naegleria
                                                   Cyst stage
         Trophozoite stage
            Flagellates
Balantidium coli (Ciliophora/ciliate)