ALKALOID FASE DIAM FASE GERAK UV REAGEN

INDOL Silika gel G Kloroform, first Dragendorff’s

toluene observing Reagent, Gives an
Aseton, under UV orange
methanol, light color with many
etanol 254 nm for types of
Ethyl acetate, the presence alkoloids.
propan-2-ol, of fluorescent 4-
and 13.5 M or quenching Dimethylaminobenzal
ammonia, i.e., zones. dehyde solution in an
100:2:1, If the latter acidic medium, such
16:3:1, and are sought, as HCl, is
9:7:4 then a layer own as Ehrlich’s
Methanol:0.2 with added reagent or Van Urk
M ammonium fluorophor reagent, and gives a
nitrate (e.g., silica blue color with simple
gel GF indoles such as the
) should be harmane series, and
used. The particularly with the
Cinchona ergot alkaloids. It has
alkaloids in been extensively used
particular to detect the alkaloids
give a very of ergot with which it
strong bright gives blue, violet,
blue or green colors.
fluorescence Cerium(IV) sulfate
under solution gives a
UV 365 nm variety
and can be of colors with the
detected at Catharanthus and
very low Vinca alkaloids,
concentration 18,19
s. Many of while iron(III)
the ergot chloride=
alkaloids perchloric acid has
give a blue been widely utilized
fluorescence for many indole and
under UV oxindole compounds,
light at both especially those from
254 and 365 Rauvolfia, Strychnos,
nm. Mitragyna,andUncaria
with which a wide
variety of colors are
seen.

SIMPLE silica gel methanol:13.5 M dragendorff’s

INDOLES ammonia 100:1.5 o-phthalaldehyde=sulfuric
acid and this can detect
amounts
as low as 20 ng for
 serotonin and 100 ng for
bufotenine.
ERGOT silica gel toluene:chlorofor UV light 254 which change to a purple
ALKALOI m:ethanol and 365 color on heating, with Van
DS 28.5:57:14.5 nm, and as blue Urk reagent.
formamide:ethano zones Iodoplatinate reagent gives
l 8:15 a
acetone:0.1 M range of colors which
ammonium enable the common
carbonate:ethanol alkaloids to be
32.5:67:5.1 distinguished from one
another, for example,
ergometrine gives a white
zone against a pinkish
background
while ergotamine gives a
strong pink color and
ergocristine a pale brown.
TERPEN Silica gel diethylamine iron(III) chloride
OIDAL 13.5 M solution=perchloric
INDOLE ammonia acid, and also ceric
ALKALOI sulfate spray
DS
variety of colors with
different types of
alkaloids
QUINO Silica gel 0% of a base UV 365 nm With iodoplatinate
LINE (e.g., dark violet reagent the four
ALKAL diethylamine in fluorescence. compounds give
OIDS a weakly polar slightly different colors
solvent such with
as chloroform) quinine giving violet-
brown, cinchonidine
grey-violet, quinidine
red-violet, and
cinchonine
brown-red.
46

INDOL 0.2 M aqueous

ES ammonium
WITH A nitrate to
QUATE methanol (2:3)
RNARY
NITRO
GEN
OTHER silica gel ethyl bright yellow
INDOL acetate:formic fluorescent
E acid:water compound
ALKAL 6:1:1 under UV
OIDS chloroform:metha light 365 nm
indoloqui nol:water 7:3:1
nolizidin chloroform:metha
 e nol 97:3
alkaloid benzene:chlorofor
cryptolep m:ethanol
ine
Canthino
ne
Camptot
hecin
Ellipticin
e

Alkaloid Isoquinolon