Research Plan Template
Name(s):
Research Plan (to Accompany Form 1A)
A) Rationale
Include a brief synopsis of the background that supports your research problem and explain why this research is
important and if applicable, explain any societal impact of your research.
The Kudzu plant is tearing down other plants all around the South causing plants to die which is also causing higher
levels of C0 to be produced in the atmosphere. We believe that instead of attempting to wipe out a whole population
of plants we change their rDNA sequences to improve live for other plants. So in order to do so we must use the
CRISPR kit for a proper transaction.
B) Research Question(s), Hypothesis(es), Engineering Goal(s), Expected
Outcomes.
How is this based on the rationale described above?
Research Question(s): Can we successfully use CRISPR to manipulate the Kudzu genes?
Hypothesis(es):If we study the patterns of the Kudzu plant and experiment on similar plants then, we can successfully
help the tress that Kudzu are tearing up.
Engineering Goal(s): To be able to help the environment
Expected Outcomes: To limit the C0 percentage in the atmosphere
Procedure(s)
Detail all procedures and experimental design including methods for data collection. Describe only your project. Do
not include work done by mentor or others.
Materials:
LB Agar
LB Strep/Kan/Arabinose Agar
Glass bottle for pouring plates
Non-pathogenic E. coli bacteria
Inoculation Loops/Plate Spreader
10-100uL variable volume adjustable pipette(1uL increments)
Box of 96 Pipette Tips
14 Petri Plates
Microcentrifuge tube rack
Nitrile Gloves
Microcentrifuge tubes
50mL Tube for measuring
Bacterial transformation buffer 25mM CalCl2, 10% PEG 8000
LB Media for transformation recovery
Cas9 plasmid
gRNA plasmid
Template DNA
Procedures: (FULL)
https://www.the-odin.com/crispr-bacterial-guide/
Risk and Safety
Identify any potential risks and safety precautions needed.
For the project we will be working with bacteria that could be harmful.
Data Analysis
Research Plan Template
Name(s):
Describe the procedures you will use to analyze the data/results.
We will analyze the effectiveness by testing it on LB Agar and LB Strep and experimenting with the DIY CRISPR kit to
familiarize ourselves with the steps and procedures.
IF Applicable
Below are subject-specific guidelines for additional items to be included in your
research plan/project summary as applicable.
Human participants research:
a. Participants: Describe age range, gender, racial/ethnic composition of participants. Identify vulnerable populations
(minors, pregnant women, prisoners, mentally disabled or economically disadvantaged).
b. Recruitment: Where will you find your participants? How will they be invited to participate?
c. Methods: What will participants be asked to do? Will you use any surveys, questionnaires or tests? What is the
frequency and length of time involved for each subject?
d. Risk Assessment: What are the risks or potential discomforts (physical, psychological, time involved, social, legal,
etc.) to participants? How will you minimize risks? List any benefits to society or participants. Keeping area clean and
ourselves protected during experimentation
e. Protection of Privacy: Will identifiable information (e.g., names, telephone numbers, birth dates, email addresses)
be collected? Will data be confidential/anonymous? If anonymous, describe how the data will be collected. If not
anonymous, what procedures are in place for safeguarding confidentiality? Where will data be stored? Who will have
access to the data? What will you do with the data after the study?
f. Informed Consent Process: Describe how you will inform participants about the purpose of the study, what they
will be asked to do, that their participation is voluntary and they have the right to stop at any time.
Vertebrate animal research:
a. Discuss potential ALTERNATIVES to vertebrate animal use and present justification for use of vertebrates.
b. Explain potential impact or contribution of this research.
c. Detail all procedures to be used, including methods used to minimize potential discomfort, distress, pain and injury
to the animals and detailed chemical concentrations and drug dosages.
d. Detail animal numbers, species, strain, sex, age, source, etc., include justification of the numbers planned.
e. Describe housing and oversight of daily care
f. Discuss disposition of the animals at the termination of the study.
Potentially hazardous biological agents research:
a. Give source of the organism and describe BSL assessment process and BSL determination.
b. Detail safety precautions and discuss methods of disposal.
E. Coil
Hazardous chemicals, activities & devices:
• Describe Risk Assessment process, supervision, safety precautions and methods of disposal.
Mrs. Curran or Mrs. Allard are supervising us when we conduct our experiments, things will be cleaned in a 10%
bleach solution before disposal, always wearing full PPE.
