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Biochemistry Postgraduate Guide

The document outlines the syllabus for a postgraduate degree in biochemistry. It covers 5 units: Unit I discusses various techniques used in biochemistry like microscopy, chromatography, spectroscopy and tracer techniques. Unit II covers the structure and chemistry of biomolecules like carbohydrates, proteins, nucleic acids and vitamins. Unit III examines metabolism of carbohydrates, lipids, amino acids and nucleic acids. Unit IV focuses on enzyme kinetics, regulation of enzymes, and mechanisms of enzyme action. Unit V looks at clinical biochemistry topics like disorders of metabolism and laboratory tests.

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121 views2 pages

Biochemistry Postgraduate Guide

The document outlines the syllabus for a postgraduate degree in biochemistry. It covers 5 units: Unit I discusses various techniques used in biochemistry like microscopy, chromatography, spectroscopy and tracer techniques. Unit II covers the structure and chemistry of biomolecules like carbohydrates, proteins, nucleic acids and vitamins. Unit III examines metabolism of carbohydrates, lipids, amino acids and nucleic acids. Unit IV focuses on enzyme kinetics, regulation of enzymes, and mechanisms of enzyme action. Unit V looks at clinical biochemistry topics like disorders of metabolism and laboratory tests.

Uploaded by

Mk.jeya
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BIOCHEMISTRY

POST GRADUATE DEGREE STANDARD PAPER -I CODE:017

UNIT I
TECHNIQUES IN BIOCHEMISTRY:-
Principles and application of light, phase contrast, Fluorescence, scanning and Transmission electron
microscopy. Cytophotometry; Gel filtration; HPLC, Ultracentrifugation; X-ray diffraction; Fluorimetry;
spectroscopy (UV, DRD/CD, visible, NMR, ESR, Atomic absorption and plasma emission); Principles and
application of tracer techniques in biology, Liquid scientillation spectrometry.

UNIT II
CHEMISTRY OF BIOMOLECULES:-
Structure of carbohydrates, Polysaccharides, Glycoproteins, Peptidoglycans, cell wall polysaccharides;
structure of aminoacids and proteins, forces involved, Ramachandran Plot. Structure of Purine,
Pyrimidine bases, Nucleotides, DNA, different types of RNA and Vitamins.

UNIT III
METABOLOSM:-
Metabolism of Carbohydrates, Aminoacids, lipids, and Nucleic acids, Respiratory chain, oxidative
phosphoerylation, free energy change; coupled reactions; Biological energy transducers; High energy
compounds and group transfer potentials; Bio energetics.

UNIT IV
ENZYMES:-
Enzyme kinetics; Regulation of enzyme activity, coenzymes; Activators/inhibitors, isoenzymes;
Mechanism of enzyme action.

UNIT V
CLINICAL BIOCHEMISTRY:-
Disorders of carbohydrate, Fat and Nitrogen, Metabolisms, Laboratory Diagnosis, Liver and Kidney
function tests; Blood coagulation disorders; Inborn errors of metabolism.

PAPER -II
UNIT I
BIOMEMBRANES:-
Structures and organization of membranes, hormones - structure, function and its role in signal
transduction, Neurotranmitters; Transport across Membranes.

UNIT II
MICROBIAL BIOCHEMISTRY:-
Classification of Micro-organisms - Viruses, bacteria, Fungi, Yeast; Basic principles of Bioprocess
technology. Fermentation products - ethanol, glycerol, lactic acid, acetone, riboflavin, vitamin B12 and
Penicillin, Preparation of media to culture micro-organisms, Soil microbiology; use of micro organisms in
sewage treatment.

UNIT III
MOLECULAR BIOLOGY:-
Prokaryotic and Kukaryotic cell structure; Eukaryotic genome organisation; cell cycle, Replication,
Transcription, Tranlation and Regulation of Gene Expression; Lysogency and Lytic cycles in
bacteriophages; bacterial transformation; transduction; conjugation and complementation; principles of
Genetic - engineering - Enzymes, vectors, C DNA and Genomic Libracy construction; Screening of
Libraries; Western, Northern and Southern blotting; Agarose Gel and SDS PAGE Electrophoresis; Dot
Blot analysis; DNA sequencing methods; Autoradiography; Transgene technology; PCR and its
application; RFLP, RAPD; Molecular Pathogenesis of Cancer.

UNIT IV
IMMUNOLOGY:-
Antigens, Immunoglobulines, T and B Lymphocytes and their characte rization; monpo - lonal antibodies;
Accessory cells - Macrophages and Dendritic cells; Purification of immunologlobulins: Ion exchange and
affinity chromatography; Enzyme Linked Immunoabsorbant Assay, vaccines; Hypersensitive actions; Auto
immunity, Antibody engineering; Antigen Presentation; Radio immunoassay.

UNIT V
TISSUE CULTURE:-
Primary cultures derived from plant, animal and human tissues, Maintenance of cell lines; Artificial
Insemination technology; Callus culture; Somaclonal Variation; Micropropagation; somatic
embryogenesis; protoplast fusion; artificial seeds.

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