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Biochemical Technique

UV visible spectroscopy is a technique that uses ultraviolet and visible light to analyze molecules by measuring how they absorb radiation in this wavelength range. There are two main types of UV visible spectroscopy: emission spectroscopy, which analyzes the wavelengths of light emitted by a substance; and absorption spectroscopy, which analyzes the wavelengths of light absorbed by a substance. The principle of UV visible spectroscopy relies on molecules absorbing UV or visible light energy to excite electrons from their ground state to higher energy states.

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Mareena Balouch
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44 views4 pages

Biochemical Technique

UV visible spectroscopy is a technique that uses ultraviolet and visible light to analyze molecules by measuring how they absorb radiation in this wavelength range. There are two main types of UV visible spectroscopy: emission spectroscopy, which analyzes the wavelengths of light emitted by a substance; and absorption spectroscopy, which analyzes the wavelengths of light absorbed by a substance. The principle of UV visible spectroscopy relies on molecules absorbing UV or visible light energy to excite electrons from their ground state to higher energy states.

Uploaded by

Mareena Balouch
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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UV VISIBLE SPETROSCOPY

CONTENT
● Definition of UV visible spectroscopy
● Types of spectroscopy
● Principle of UV Visible spectroscopy
● Absorbance Laws
● Instrumentation
What is UV VISIBLE SPECTROSCOPY
● The absorption of UV ( 200_400nm) and visible ( 400_800nm) radiations
by molecules is associated with the excitation of valency electrons from
ground state to higher energy state
Types of UV VISIBLE SPECTROSCOPY
There are two types of UV Visible spectroscopy

● Eission spectroscopy
● Absorbstion spectroscopy

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