The Effectiveness of
Welcome Plant
(Zamioculas zamiifolia)
Leaf Extract against
Golden Kuhol.
Researchers:
Maria Lucille Ines Mercado
Jedeah Michelle Savellano
RhayZhan Stephen Pugal
The Effectiveness of Welcome Plant (Zamioculas zamiifolia) Leaf Extract against Golden Kuhol.
General Problem:
This study will be conducted to determine the effectiveness of Welcome plant
(Zamioculas zamiifolia) leaf extract against Golden Kuhol.
Specifically, it will seek to answer the following questions:
1. How effective is the Welcome plant leaf extract in killing Golden kuhol?
2. What is the most effective concentrations of Welcome plant leaf extract in terms
of the number of dead Golden kuhol?
3. . Is there a significant difference between and among the treatment used in terms
of the number of dead Golden kuhol?
Hypothesis:
1. The Welcome plant leaf extract is effective in killing Golden kuhol.
2. 100% extract is the most effective concentration of Welcome plant leaf extract in
terms of the number of dead Golden kuhol.
3. There is a significant difference between and among the treatments in terms of
the number of dead Golden kuhol.
I. Preparation of the Welcome plant leaf extract:
Five (5) kg of Welcome plant (Zamioculas zamiifolia) leaves will be gathered
from Barangay Teppeng, Sinait, Ilocos Sur. The Welcome plant (Zamioculas
zamiifolia) leaves will be washed with tap water and chopped into small pieces
using a knife. Then the chopped Welcome plant leaves will be blended then will
be filtered to extract the juice and the juice to be obtained will be placed in clean
container.
II. Preparation of Treatments
Six treatments will be prepared based from the following concentrations of
Welcome plant (Zamioculas zamiifolia) leaf extract as follows:
Treatment 1: 100% Welcome plant (Zamioculas zamiifolia) leaf extract
Treatment 2: 75% extract (75 ml extract and 25 ml distilled water)
Treatment 3: 50% extract (50 ml extract and 50 ml distilled water)
Treatment 4: 25% extract (25 ml extract and 75 ml distilled water)
Treatment 5: Positive control (Commercial Pesticide)
Treatment 6: Negative control (Distilled Water)
III. Preparation of the Test Organism
One hundred-eighty (180) Golden kuhol will be collected from Barangay
Purag, Sinait, Ilocos Sur and this will be kept on a pail of tap water. The Golden
kuhol will be classified according to their sizes. One hundred-eighty Golden
kuhols of the same sizes will be divided into six and placed into eighteen separate
containers each with 10 Golden kuhol and 5L of tap water.
IV. Treatment Application
The Welcome plant (Zamioculas zamiifolia) leaf extract will be tested to one
hundred-eighty Golden kuhol into six treatments in three replicates. One hour
after placing the Golden kuhol in each container, 70 ml of each concentration will
be placed in each treatment. Observation will be done in 30 minutes to determine
the death of the Golden kuhol.
V. Number of dead Golden Kuhol will be counted thirty (30) minutes after treatment.
Treatments Replication 1 Replication 2 Replication 3
Treatment 1
Treatment 2
Treatment 3
Treatment 4
Treatment 5
Treatment 6
VI. Static Analysis
The data to be gathered will be summarized in a table and analyzed using
ANOVA. Likewise, graphical representation will be used for further analysis.
VII. Bibliography
https: //en.m.wikipedia.org
www.knowledgebank.irri.org