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In biochemistry, a metabolic pathway is a linked series of chemical reactions occurring within
a cell. The reactants, products, and intermediates of an enzymatic reaction are known
as metabolites, which are modified by a sequence of chemical reactions catalyzed by enzymes.
[1]:26
In most cases of a metabolic pathway, the product of one enzyme acts as the substrate for
the next. However, side products are considered waste and removed from the cell. [2] These
enzymes often require dietary minerals, vitamins, and other cofactors to function.
Different metabolic pathways function based on the position within a eukaryotic cell and the
significance of the pathway in the given compartment of the cell. [3] For instance, the, electron
transport chain, and oxidative phosphorylation all take place in the mitochondrial membrane. [4]:73, 74
& 109
In contrast, glycolysis, pentose phosphate pathway, and fatty acid biosynthesis all occur in
the cytosol of a cell.[5]:441–442
There are two types of metabolic pathways that are characterized by their ability to either
synthesize molecules with the utilization of energy (anabolic pathway) or break down of complex
molecules by releasing energy in the process (catabolic pathway).[6] The two pathways
complement each other in that the energy released from one is used up by the other. The
degradative process of a catabolic pathway provides the energy required to conduct a
biosynthesis of an anabolic pathway.[6]In addition to the two distinct metabolic pathways is the
amphibolic pathway, which can be either catabolic or anabolic based on the need for or the
availability of energy.[7]