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Changing The Pump Seals: (All Pumps Except The G1361A Preparative Pump)

The document provides instructions for changing pump seals and inspecting sapphire pistons on Agilent pumps. Key steps include: 1. Disassembling the pump head and removing the two seals using a piston and solvent. 2. Cleaning the pump chambers and seal areas, then inserting new seals. 3. Performing a seal wear-in procedure for standard seals that involves flushing with isopropanol. 4. Inspecting pistons for scratches when replacing seals and replacing scratched or damaged pistons.

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91 views2 pages

Changing The Pump Seals: (All Pumps Except The G1361A Preparative Pump)

The document provides instructions for changing pump seals and inspecting sapphire pistons on Agilent pumps. Key steps include: 1. Disassembling the pump head and removing the two seals using a piston and solvent. 2. Cleaning the pump chambers and seal areas, then inserting new seals. 3. Performing a seal wear-in procedure for standard seals that involves flushing with isopropanol. 4. Inspecting pistons for scratches when replacing seals and replacing scratched or damaged pistons.

Uploaded by

sarasa
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Changing the Pump Seals

(All pumps except the G1361A preparative pump)

1. Disassemble the pump head and use


a piston to remove the two seals.
2. Clean the pump chambers and seal
areas by flushing with solvent
and wiping with a lint-free cloth.
Pump seals should be replaced when there 3. Insert new seals.
are leaks on the bottom of the pump head,
when retention times are inconsistent, 4. Reassemble the pump head.
or when the pressure ripple is unstable. 5. Perform the seal wear-in procedure
(Standard seals 5063-6589 only).

The wear-in procedure involves flushing


the seals with isopropanol at high pressure
while they are in place.

42 Order online at www.agilent.com/chem/store


Pump Maintenance

Changing and Inspecting the Sapphire Pistons


(All pumps except the G1361A preparative pump)

Each time you replace the seals, examine 1. Disassemble the pump head assembly.
the pistons for scratches, which can cause
2. Remove any deposits – particularly
small, but damaging leaks.
buffers – from the piston surface using
alcohol or toothpaste. Replace the
pistons if scratched.
3. Rinse thoroughly in alcohol and
HPLC-grade water.
4. Reassemble the pump head.
5. Check to make sure there are no
fractures in the springs. Replace
piston housing assembly if springs
are damaged.
6. Reinsert the pistons.

Seals and Pistons


Description Part No.

Sapphire Piston 5063-6586


Piston Seals, graphite filled Teflon (reversed phase), 2/pk 5063-6589
Piston Seals, polyethylene (normal phase), 2/pk 0905-1420
Piston housing assembly (includes springs) G1311-60002
Extended PM kit 5065-4499
Contains 5063-6589 piston seals (2), 01018-22707 PTFE frits (5/pk),
5062-8562 cartridge active inlet valve, G1311-60012 outlet ball valve
& 5063-6586 pistons (2)

Order online at www.agilent.com/chem/store 43

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