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Carbohydrate Tests for Students

This lab report summarizes an experiment on qualitative tests for carbohydrates. Key results include that the Molisch test identifies carbohydrates by producing a purple color ring, and that Barfoed's test distinguishes monosaccharides from disaccharides based on reaction speed. The Bial's test identifies pentoses by turning the solution blue-green. A table predicts the results of common carbohydrates on each qualitative test.

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0% found this document useful (0 votes)
742 views3 pages

Carbohydrate Tests for Students

This lab report summarizes an experiment on qualitative tests for carbohydrates. Key results include that the Molisch test identifies carbohydrates by producing a purple color ring, and that Barfoed's test distinguishes monosaccharides from disaccharides based on reaction speed. The Bial's test identifies pentoses by turning the solution blue-green. A table predicts the results of common carbohydrates on each qualitative test.

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School of Industrial Technology

Faculty of Applied Sciences

________________________________________________________

BIO 462
Laboratory Title:
Practical 6: Qualitative test for carbohydrate
Date of experiment: 08/05/2020
Laboratory Group: Tuesday

Name : NURUL AINAA AQILAH BINTI SAIFOLBAHARI


Student ID : 2018438786
Group : AS2462A

MARK:
Experiment 6: Qualitative Test for Carbohydrates

Objective: The objective of this lab is to know different methods for qualitative estimation
and identification of carbohydrates.

a. In the Molisch Test, what is the expected result that would occur in the presence of
carbohydrates?
 In the presence of carbohydrate, the expected result would be formation of purple
colour ring,

b. What are the differences between Barfoed’s test and Benedict’s test?
 The difference between Barfoed’s test and Benedict’s objective of the test was done.
 As for the Benedict’s test, it was done distinguish between reducing and non
reducing sugar by determine the presence of reducing sugar.
 The Barfoed’s test, it was done to distinguish between monosaccharide and
disaccharide. The reaction Barfoed’s reagent with monosaccharide is faster than
disaccharide.
 Besides, the copper reduction process for Barfoed’s test, occur in acidic medium
with the pH of 4.6 rather than alkaline compared to Benedict’s test.

c. In the Bial’s Test, which type of sugar would result in a blue-green solution?
 The sugar that would result in a blue-green solution is the pentoses.
d. Predict the expected results of the following carbohydrates. Use (+) for positive result
and (-) for negative result.

Types of Carbohydrate

Test Glucose Fructose Lactose Maltose Sucrose Ribose Starch

Molisch + + + + + + +

Benedict + + + + - + -

Barfoed + + - - - + -

Seliwanoff - + - - + - -

Bial - + - - + + -

Iodine - - - - - - +

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