School of Industrial Technology
Faculty of Applied Sciences
________________________________________________________
                   BIO 462
                  Laboratory Title:
   Practical 6: Qualitative test for carbohydrate
         Date of experiment: 08/05/2020
             Laboratory Group: Tuesday
Name          :    NURUL AINAA AQILAH BINTI SAIFOLBAHARI
Student ID    :    2018438786
Group         :    AS2462A
                                      MARK:
Experiment 6: Qualitative Test for Carbohydrates
Objective: The objective of this lab is to know different methods for qualitative estimation
and identification of carbohydrates.
 a.   In the Molisch Test, what is the expected result that would occur in the presence of
      carbohydrates?
         In the presence of carbohydrate, the expected result would be formation of purple
          colour ring,
 b.   What are the differences between Barfoed’s test and Benedict’s test?
         The difference between Barfoed’s test and Benedict’s objective of the test was done.
         As for the Benedict’s test, it was done distinguish between reducing and non
          reducing sugar by determine the presence of reducing sugar.
         The Barfoed’s test, it was done to distinguish between monosaccharide and
          disaccharide. The reaction Barfoed’s reagent with monosaccharide is faster than
          disaccharide.
         Besides, the copper reduction process for Barfoed’s test, occur in acidic medium
          with the pH of 4.6 rather than alkaline compared to Benedict’s test.
 c.   In the Bial’s Test, which type of sugar would result in a blue-green solution?
         The sugar that would result in a blue-green solution is the pentoses.
d.     Predict the expected results of the following carbohydrates. Use (+) for positive result
       and (-) for negative result.
                                              Types of Carbohydrate
      Test         Glucose      Fructose   Lactose    Maltose    Sucrose     Ribose      Starch
 Molisch              +               +       +          +          +          +            +
 Benedict             +               +       +          +           -         +             -
     Barfoed          +               +       -          -           -         +            -
Seliwanoff             -              +       -          -          +           -           -
      Bial             -              +       -          -          +          +            -
     Iodine            -              -       -          -           -          -           +