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Buffer Solution Challenge

This document discusses buffer capacity and the preparation of an isotonic buffered preserved solution. It provides the calculation to determine the capacity of a buffer containing 0.20 mole of acetic acid and sodium acetate per liter when 0.01 M NaOH is added. It also gives the recipe and instructions to make a 100 mL Sorensen phosphate buffered isotonic solution, including adding 0.7% chlorobutanol as a preservative and calculating the amount needed based on its volume. A table with the mixtures of monobasic and dibasic sodium phosphate solutions and resulting pH and NaCl amounts is included.

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Rhita Tamale
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192 views1 page

Buffer Solution Challenge

This document discusses buffer capacity and the preparation of an isotonic buffered preserved solution. It provides the calculation to determine the capacity of a buffer containing 0.20 mole of acetic acid and sodium acetate per liter when 0.01 M NaOH is added. It also gives the recipe and instructions to make a 100 mL Sorensen phosphate buffered isotonic solution, including adding 0.7% chlorobutanol as a preservative and calculating the amount needed based on its volume. A table with the mixtures of monobasic and dibasic sodium phosphate solutions and resulting pH and NaCl amounts is included.

Uploaded by

Rhita Tamale
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Buffer Capacity and Preparation of Isotonic Buffered Preserved

Solution

¾ Solve this problem

At a hydrogen ion concentration of 1.75 * 10-5 (pH = 4.76), what is the capacity of
buffer containing 0.20 mole of each of acetic acid and sodium acetate per liter of
solution if 0.01 M NaoH is added?

¾ Preparation of isotonic buffered preserved solution:

a. Prepare a 100 ml of Sorensen Phosphate buffered isotonic solutions, using


table#1.
Monobasic sodium phosphate solution = 8.00 g / 1000 ml
Dibasic sodium phosphate solution = 9.47 g / 1000 ml.

b. Add Chlorobutanol 0.7% as a preservative. Do the required calculation before


its addition. (V-value of 0.3 g of Chlorobutanol is 8 ml)
N.B. calculate the required amount of the chemicals in step (a) and (b).

Monobasic Dibasic S.P.S. Resulting NaCl to render


Sodium Phosphate “mL” buffer solution isotonic “g”
Solution “mL” pH “mL”
90 10 5.9 0.52
80 20 6.2 0.51
70 30 6.5 0.50
60 40 6.6 0.49
50 50 6.8 0.48
40 60 7.0 0.46
30 70 7.2 0.45
20 80 7.4 0.44
10 90 7.7 0.43
5 95 8.0 0.42

Table # 1:Sorensen Phosphate Buffer Solution

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