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Abb Table and Figure

This document contains lists of symbols, abbreviations, tables and figures used in a study investigating the interaction between autophagy and apoptosis in carboplatin-treated lung cancer cells. It includes a list of 14 symbols and abbreviations commonly used in cell biology and cancer research. There are also brief descriptions of 3 tables and 6 figures to be included in the study results, related to molecular docking of carboplatin, effects on cell viability, gene and protein expression analysis, and fluorescence microscopy images of markers for autophagy and apoptosis.

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NATIONAL XEROX
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87 views5 pages

Abb Table and Figure

This document contains lists of symbols, abbreviations, tables and figures used in a study investigating the interaction between autophagy and apoptosis in carboplatin-treated lung cancer cells. It includes a list of 14 symbols and abbreviations commonly used in cell biology and cancer research. There are also brief descriptions of 3 tables and 6 figures to be included in the study results, related to molecular docking of carboplatin, effects on cell viability, gene and protein expression analysis, and fluorescence microscopy images of markers for autophagy and apoptosis.

Uploaded by

NATIONAL XEROX
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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LIST OF SYMBOLS AND ABBREVIATIONS

 µl Microlitre

 µg Microgram

 ml Milliliter

 mg Milligram

 mM Milimolar

 µM Micromolar

 rpm Revolution per minute

 DMSO Dimethyl sulphoxide

 PBS Phosphate buffer saline solution

 DMEM Dulbecco modified eagles media

 ROS Reactive oxygen species

 MMP Mitochondrial Membrane Potential

 PCD Programmed cell death

 ATP Adenosine tri phosphate

 DNA Deoxy ribose nucleic acid

 RNA Ribose nucleic acid

 PS Phosphatidylserine

 NCCS National Centre for Cell Sciences

 DCF-DA Dichlorodihydrofluorescein di-acetate

 PCR Polymerase chain reaction

 EDTA Ethylenediaminetetraacetic acid

i
 SD Standard deviation

 MIC Minimum inhibitory concentration

 5FU 5-Fluorouracil

ii
LIST OF TABLES

S.No. Name of Table Page No.

1.1 Lung cancer statistics in India for the year 2018. 1

3.1 Sequences of primers used in the study 21-22

4.1 Binding energy of the complex of carboplatin 24


with LC3 and caspase3, and details of inhibition
constant and interacting amino acids.
4.2 Relative change in the expression of apoptosis 29
and autophagy marker gene as observed in
carboplatin treated A549 lung cancer cells
before and after autophagy inhibition..

iii
LIST OF FIGURES

S.No. Name of Figure Page No.

1.1 Proposed tumor suppressing and tumor- 4


promoting roles of autophagy in cancer.

2.1 Development of cancer 6

2.2 Intrinsic and Extrinsic Apoptotic Pathways 11

2.3 Mechanism of Autophagy 13

3.1 Grids in heamocytometer 19

4.1 Molecular docking interaction of carboplatin 23


with (a) LC3 and (b) Caspase3

4.2 Dose-dependent inhibition of A549 lung cancer 24


cells by carboplatin after 24 h of treatment, as
assessed by MTT assay. The IC50 was
determined to be 100 µM.

4.3 (a) Gel image depicting the expression of LC3 25


before and after autophagy inhibition. (b)
Graphical representation of the change in LC3
mRNA expression after autophagy inhibition.

4.4 (a) Fluorescence micrographs of reactive 26


oxygen species generation in A549 cells after
treatment with carboplatin under different
conditions for 24 h. (b) Graphical representation
of fluorescence intensity of DCF-DA staining in
A549 cells, as quantified using Image J
software.

4.5 (a) Fluorescence micrographs showing changes 27


in mitochondrial membrane potential in A549
lung cancer cells after treatment with

iv
carboplatin under different conditions for 24 h.
(b) Graphical representation of fluorescence
intensity of Mito Tracker Red staining in A549
cells, as quantified using Image J software.

4.6 (a) Changes in the expression of autophagy and 28


apoptosis marker genes in carboplatin-treated
A549 cells before and after autophagy
inhibition, as observed by RT-PCR analysis.
Relative change in expression of autophagic and
apoptotic marker genes, (b) BAD and BCL x(L)
(c) LC3 and CASPASE3. (1) Control (2)
Carboplatin (3) Control (starved)(4) Carboplatin
(A(autophagy)⊥)

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