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Officinale) Which Exhibited Its Anti-Angiogenic Property by Decreasing The CAM

The chick Chorioallantoic Membrane (CAM) is a double layer of mesoderm that is highly vascularized and connects to the embryonic circulation. It is frequently used to study angiogenesis in vivo by assessing the effects of test substances on vascular density, occlusion, and sprouting. Numerous studies have used the CAM assay to evaluate anti-angiogenic properties of various compounds, such as PSP-2 which suppressed vessel branching, and crenolanib which decreased sprout length. The CAM assay is advantageous for its rapid development, inexpensive chicken eggs, and lack of innervation which avoids ethical issues regarding pain in embryos.

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Carlos Niño
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72 views2 pages

Officinale) Which Exhibited Its Anti-Angiogenic Property by Decreasing The CAM

The chick Chorioallantoic Membrane (CAM) is a double layer of mesoderm that is highly vascularized and connects to the embryonic circulation. It is frequently used to study angiogenesis in vivo by assessing the effects of test substances on vascular density, occlusion, and sprouting. Numerous studies have used the CAM assay to evaluate anti-angiogenic properties of various compounds, such as PSP-2 which suppressed vessel branching, and crenolanib which decreased sprout length. The CAM assay is advantageous for its rapid development, inexpensive chicken eggs, and lack of innervation which avoids ethical issues regarding pain in embryos.

Uploaded by

Carlos Niño
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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The chick Chorioallantoic Membrane (CAM) is a double layer of

mesoderm formed by the fusion of the allantois and the chorion of the developing
embryo. This double layer is enriched with vascular supply, which connects to the
embryonic circulation via the allantoic arteries and veins (Ribatti, 2001). Due to
this property, CAM is frequently used for in vivo study of angiogenesis.

An effect on angiogenesis is indicated by a change in vascular density in


and around the CAM as a result of the addition of test substances applied to it
(Irvin, 2015, p.7). In terms of anti-angiogenesis, a positive result is indicated by
vascular occlusion (Nowak-Slowinska, 2015, p. 11), while pro-angiogenic effect
is measured by growth of the vessels and sprouting ability (Manjunathan, 2015, p.
4).

Numerous studies about anti-angiogenic properties of different substances


utilized the CAM assay. One of the substances assessed for its anti-angiogenic
potential is the PSP-2
(1,2bis(bis(dimethylphosphorothioylmethyl)phosphino)ethane), a copper chelator
by observing that it suppressed the branching of the vessels (Heuberger, 2019,
p.8).

Crenolanib, a tyrosine kinase inhibitor, showed anti-angiogenic effects by


decreasing the average sprout length (Berndsen, 2019).

Another study observed the ability of opioids in inhibiting angiogenesis by


observing the decrease of capillary density after administration in different
concentrations (Karaman, 2017).

Strong anti-angiogenic activity is also exhibited by bioactive compounds


of different species of plants using CAM assay, among those is Ginger (Zinciber
officinale) which exhibited its anti-angiogenic property by decreasing the CAM
region and blood vessel diameter. The decreased CAM region is proportional the
angiogenesis activity (Bashir, 2017).

The CAM assay offers many advantages, the most prominent of which is
the accessibility and rapid growth, as the CAM develops in a relatively short
period of time, making it suitable for studies involving developmental pathways
(Nowak-Sliwinska, 2015, p.3). This also adds to the fact that chicken eggs are
inexpensive, making the method suitable for large scale screening (Stryker, 2019,
p. 9). The CAM lacks innervation, making the embryo insensitive to pain. This
offers an advantage because ethical issues does not pose as a limitation (Kunz,
2019, p.2).

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