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Total Magnification of Microscope Principle of Magnification in Microscope

The document discusses the principles and mechanisms of magnification in light microscopes. It explains that light microscopes use lenses to magnify small objects, with objective lenses providing magnifications of 4x, 10x, or 40x. The total magnification is calculated by multiplying the objective lens magnification by the eyepiece magnification of 10x. Higher magnifications allow viewing closer details but require shorter working distances that risk damaging samples or lenses. In summary, light microscopes are important tools that use lenses and magnification to study biological structures too small to see with the naked eye.

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Lingesswari Nagarajan
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263 views1 page

Total Magnification of Microscope Principle of Magnification in Microscope

The document discusses the principles and mechanisms of magnification in light microscopes. It explains that light microscopes use lenses to magnify small objects, with objective lenses providing magnifications of 4x, 10x, or 40x. The total magnification is calculated by multiplying the objective lens magnification by the eyepiece magnification of 10x. Higher magnifications allow viewing closer details but require shorter working distances that risk damaging samples or lenses. In summary, light microscopes are important tools that use lenses and magnification to study biological structures too small to see with the naked eye.

Uploaded by

Lingesswari Nagarajan
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Magnification of Light Microscope

Introduction:
The light microscope, also referred to as a optical microscope, is a type of microscope that commonly uses visible light and a system of lenses to
generate magnified images of small objects. The objective lenses on a compound light microscope does have powers that start of as 4x on the smallest
power, 10x on the middle power setting and 40x on the maximum power setting. This means that the object can be magnified either, 40x, 100x or
400x. Throughout the development of light microscope magnification of light has increased, but very high magnifications are not possible. The
maximum magnification with a light microscope is around ×1500.

Principle of magnification in microscope Total Magnification of microscope


Light microscope magnifies the size of the object How to determine the TOTAL MAGNIFICATION of your
by a complex system of lens arrangement. It has a microscope:
series of two lenses; the objective lens close to the a. This is calculated by multiplying the magnification of the
object to be observed and,the ocular lens or Eyepiece(10x) by the magnification of the Objective lens that
eyepiece. you are using(4x, 10x, or 40x).

Total Magnification = Eyepiece Magnification(10x) × Objective


Lens Power
Example: You are looking at a specimen through your
LOW Power Lens Total Magnification = (10x) × (4x) = 40x

Light from a light source (mirror or electric lamp)


passes through a thin transparent object(Figure4.4)

The objective lens produces a magnified ‘real image’ Magnification of Image (solving magnification problems and
first image) of the object the relative sizes of object e.g. a cell and its observed image in a
microscope.)
This image is again magnified by the ocular lens The formula 'triangle' for magnification is shown below:
(eyepiece) to obtain a magnified ‘virtual image’
(final image)

Importance of Magnification

 For light microscopes, the higher the


magnification, the closer the lens must be
positioned to the object being observed.
 If the lens gets too close, it may crash into the
specimen, destroying the slide or specimen and
possibly damaging the lens, so exercise great
care when using magnifications over 100x.

Conclusion:
In a nutshell, light microscope clearly is the high-
powered tool for understanding the structure and
function of tissues. On the other hand, magnification
Team Members: refers to the process of enlarging the apparent size and
1.Kvoosik A/L Narayanan (E20181020668) not the physical size of something It also commonly
used in biomedical science courses, research and
2.Pramilla A/P Periasamy (E20182022989)
diagnostic laboratories.
3.Chaavenea A/P Poobalan (E20182022973)

4.Divyarsi A/P Thellaivassan (E20182022971)

5.Lingesswari A/P Nagarajan (E20182022985)

6.Muhamad Amirul Shah Bin Jaafar (E20182022993)

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