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Isopropyl Myristate

This document provides the procedure for analyzing isopropyl myristate using gas chromatography. It describes preparing a system suitability solution and test solution, and outlines the operational parameters and chromatography conditions used, including the column, inlet, detector settings, and temperature programs. It specifies that the relative retention times for isopropyl myristate and palmitate peaks should be 1.0 and 1.3 respectively, with resolution over 6.0 and tailing factor under 2.0. The percentage of isopropyl myristate in the test solution is then calculated from the peak responses.

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Vindy Meryana
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151 views2 pages

Isopropyl Myristate

This document provides the procedure for analyzing isopropyl myristate using gas chromatography. It describes preparing a system suitability solution and test solution, and outlines the operational parameters and chromatography conditions used, including the column, inlet, detector settings, and temperature programs. It specifies that the relative retention times for isopropyl myristate and palmitate peaks should be 1.0 and 1.3 respectively, with resolution over 6.0 and tailing factor under 2.0. The percentage of isopropyl myristate in the test solution is then calculated from the peak responses.

Uploaded by

Vindy Meryana
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Isopropyl Myristate

Assay (GC method): Not less than 90.0%


[Kadar (metode KG: tidak kurang dari 90,0%]
System suitability solution:
[Larutan kesesuaian sistem]:
Transfer 25.0mg of Isopropyl Myristate Standard and 2.5mg of Isopropyl Palmitate Standard,
accurately weighed, to a 5-ml volumetric flask, dissolve in, dilute with n-hexane to volume,
and mix well.
[Pindahkan 25,0mg baku isopropil miristat dan 2,5mg isopropil palmitat, timbang secara
seksama, masukkan ke dalam labu ukur 5mL, larutkan dalam n-heksan sampai batas
volume, dan aduk rata].
Test solution:
[larutan uji]:
Transfer 50.0mg of sample, accurated weighed, to a 10-ml volumetric flask, dissolve in,
dilute with n-hexane to volume, and mix well.
[Transfer 50,0mg sampel, timbang secara saksama, ke dalam labu ukur 10ml, larutkan
didalamnya, encerkan dengan n-heksan sampai batas volume, dan aduk rata.]

Procedure:
[Prosedur]:
Apparatus: Gas Chromatography (GC) with Flame - Ionization Detector.
[Peralatan: Kromatografi gas (KG) dengan detektor ionisasi api (FID)]
Operational parameters:
[Parameter operasional]:
Column : Agilent J & W G27 Column HP-5 0.25µm (0.32mm ID x 30m)
[Kolom]
Inlet : Temperature = 240°C; Split ratio = 10 : 1
[“Inlet”] [Suhu = 240°C; “Split ratio” = 10 : 1
Column temperature : Initial Temp. Temp. ramp Final Temp. Hold Time at Final
programs (°C) (°C)/min (°C) Temp. (min)
[Program suhu [Suhu awal [Kenaikan suhu [Suhu akhir [Jeda waktu pada
kolom] (°C)] (°C/menit)] (°C)] suhu akhir
(menit)]
150 - 150 1
150 6 230 11
(Make adjustments if necessary)
[buat pengaturan bila diperlukan]
Carrier gas: Nitrogen, flow rate: 1.5ml/min.
[Gas pembawa: Nitrogen, laju alir : 1,5ml/menit].
Detector : Flame Ionization Detector
[Detektor] [Detektor ionisasi api]
Temperature = 280°C
[Suhu = 280°C]
H2 flow rate = 40ml/min
[Laju alir H2 = 40ml/menit]
Air flow rate = 450ml/min
[Laju alir udara = 450ml/menit]
Makeup N2 flow rate = 20ml/min
[Laju alir “Makeup N2” = 20ml/menit]

Inject about 2µl of Solvent (n-hexane), System suitability solution, and Test solution into GC.
[Injeksikan sekitar 2 µl pelarut (n-heksan), larutan kesesuaian sistem 1, dan larutan uji
ke dalam KG].

Chromatographic system:
[Sistem kromatografi]:
Chromatograph the System suitability solution, and record the peak responses as directed
for Procedure:
[Kromatografikan larutan kesesuaian sistem, dan rekam respon puncak seperti yang
diuraikan untuk prosedur]:
1) The Relative retention times for Isopropyl myristate and Isopropyl palmitate are 1.0 and
1.3, respectively.
[Waktu retensi relatif untuk isopropil miristat dan isopropil palmitat masing-masing
adalah 1,0 dan 1,3.]
2) The resolution, R, between Isopropyl myristate and Isopropyl palmitate peaks is not less
than 6.0.
[Resolusi, R, antara puncak isopropil miristat dan isopropil palmitat tidak kurang
dari 6,0].
3) The tailing factor for Isopropyl myristate peak is not more than 2.0.
[Faktor “tailing” untuk puncak isopropyl miristat tidak lebih dari 2,0].
4) The relative Standards Deviation (RSD) for 6 replicate injections is not more than 2.0%.
[Simpangan baku relatif (RSD) untuk 6 replikasi injeksi tidak lebih dari 2,0%].

Calculation:
[Perhitungan]:
Calculate the percentage of the Isopropyl Myristate (C 17H34O2) in the portion of test solution
taken:
[Hitung persentase isopropil Miristat (C17H34O2) dalam larutan uji dengan rumus]:

(rU/rT) × 100
in which:
[di mana];
rU is the peak response of isopropyl myristate.
[rU adalah respon puncak isopropil miristat].
rT is the sum of all the peaks.
[rT adalah jumlah keseluruhan puncak].

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