14
1. Which of the following is not true concerning eukaryotic mRNA processing?
A. a
ddition of a 3′ poly-A tail
B. a
ddition of a 5′ cap
C. intron splicing
D. occurs in the nucleus
E. o
ccurs in the cytoplasm
2. Which of the following experiments was useful in determining the existence of introns in the
adenovirus genome?
A. D
NA/DNA hybridization
B. R
NA/RNA hybridization
C. R-looping experiments
D. RT-PCR
E. c DNA cloning experiments
3. You have isolated a new species of mRNA using an oligo-dT column. You then perform an R-
looping experiment to determine if the gene encoding for your mRNA contains introns. Below is a
representation of the electron micrograph results. Which of the following can you conclude from
your experiment?
A. T
he gene contains no introns.
B. T
he gene contains one intron.
C. The gene contains two introns.
D. The gene contains three introns.
E. T
he results are inconclusive.
4. The first two bases and the last two bases in the splicing signal consensus sequence are
A. G
T-AG.
B. G
U-AG.
C. CU-AG.
D. GU-AC.
E. G
T-TG.
5. Which of the following is the name for the Yeast 40S particle where mRNA splicing takes place?
A. r ibosome
B. n
ucleolus
C. spliceosome
D. hnRNA
E. R -loop
6. "Snurps" are composed of
A. R
NA.
B. p
rotein.
C. DNA.
D. RNA and protein.
E. D
NA and RNA.
7. Which of the following snRNP is mismatched with its function?
A. U
1: base pairs with 5′ splice site of mRNA.
B. U
2: base pairs with the conserved sequence at splicing branch point.
C. U4: base pairs with 3′ splice site of mRNA.
D. U5: associates with last nucleotide in one exon and the first nucleotide in the next exon.
E. U
6: base pairs with 5′ end of the intron.
8. Which of the following complexes found in the spliceosome cycle is mismatched with its
snRNP(s)?
A. c ommitment complex: U6/U4
B. A
complex: U1 and U2
C. B1 complex: all snRNPs
D. B2 complex: U2, U5 and U6
E. I complex: U2, U5 and U6
9. Which of the following splicing factors is required for the correct 3′ splice site selection?
A. S
C35
B. S
lu7
C. U11
D. SF2
E. S
Rp55
10. The catalytic center of the spliceosome appears to include
A. M
g2+.
B. U
2 and U6 snRNP.
C. the branch point region of the intron.
D. Mg2+ and the branch point region of the intron.
E. M
g2+, U2, and U6 snRNP, and the branch point region of the intron.
11. Which of the following is the first snRNP to bind during the assembly stage of the spliceosome
cycle?
A. U
1
B. U
2
C. U4
D. U5
E. U
6
12. The binding of which of the following snRNPs to spliceosome requires ATP?
A. U
1
B. U
2
C. U4
D. U5
E. U
6
13. The expressed regions of genes are referred to as ________ while the noncoding regions are
called_______.
________________________________________
14. The process by which introns are removed from immature mRNA is called _____________.
________________________________________
15. _______________ disrupting the normal splicing consensus sequence are one way to obtain
abnormal splicing patterns.
________________________________________
16. Joan Steitz was one of the researchers who used __________ studies to demonstrate that U6
snRNP binds to the 5′ end of the intron.
________________________________________
17. Some introns, such as Group II introns, are self splicing. These types of introns contain catalytic
RNA's, also referred to as __________.
________________________________________
18. Exon definition involves the use of the ________ subunit of RNA polymerase II.
________________________________________
19. Alternative splicing is involved in _________ determination in fruit flys.
________________________________________
20. There are a common set of ___________ Sm proteins that are specific to each snRNA.
________________________________________
21. Introns are regions found in DNA sequences and mature mRNA sequences.
True False
22. RNA polymerase II does not transcribe through introns, instead it jumps over them.
True False
23. Heterogeneous nuclear RNA (hnRNA) is thought to be a precursor of mRNA.
True False
24. Pre-mRNA splicing occurs in the cytoplasm.
True False
25. Experimental evidence demonstrates that mRNA precursors are spliced via a lariat-shaped
intermediate.
True False
26. Base pairing between the 5′ splice site and U1 snRNA is the only interaction essential for
splicing.
True False
27. The spliceosome cycle is one way for a cell to regulate gene expression.
True False
28. Immature mRNA splicing can be established by either exon definition or intron definition.
True False
29. Alternative splicing patterns can lead to the production of different protein products from the same
gene.
True False
30. SC35 is an RNA binding protein that is responsible for the commitment to splice the β-globin pre-
mRNA.
True False
31. The discovery of alternate splicing helped prove the "one gene, one protein" theory.
True False
14 Key
1. Which of the following is not true concerning eukaryotic mRNA processing?
A. addition of a 3′ poly-A tail
B. addition of a 5′ cap
C. intron splicing
D. occurs in the nucleus
E. occurs in the cytoplasm
Blooms Level: 2. Understand
Section: 14.01
Weaver - Chapter 14 #1
2. Which of the following experiments was useful in determining the existence of introns in the
adenovirus genome?
A. DNA/DNA hybridization
B. RNA/RNA hybridization
C. R-looping experiments
D. RT-PCR
E. cDNA cloning experiments
Blooms Level: 2. Understand
Section: 14.01
Weaver - Chapter 14 #2
3. You have isolated a new species of mRNA using an oligo-dT column. You then perform an R-
looping experiment to determine if the gene encoding for your mRNA contains introns. Below
is a representation of the electron micrograph results. Which of the following can you conclude
from your experiment?
A. The gene contains no introns.
B. The gene contains one intron.
C. The gene contains two introns.
D. The gene contains three introns.
E. The results are inconclusive.
Blooms Level: 5. Evaluate
Section: 14.01
Weaver - Chapter 14 #3
4. The first two bases and the last two bases in the splicing signal consensus sequence are
A. GT-AG.
B. GU-AG.
C. CU-AG.
D. GU-AC.
E. GT-TG.
Blooms Level: 1. Remember
Section: 14.01
Weaver - Chapter 14 #4
5. Which of the following is the name for the Yeast 40S particle where mRNA splicing takes
place?
A. ribosome
B. nucleolus
C. spliceosome
D. hnRNA
E. R-loop
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #5
6. "Snurps" are composed of
A. RNA.
B. protein.
C. DNA.
D. RNA and protein.
E. DNA and RNA.
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #6
7. Which of the following snRNP is mismatched with its function?
A. U1: base pairs with 5′ splice site of mRNA.
B. U2: base pairs with the conserved sequence at splicing branch point.
C. U4: base pairs with 3′ splice site of mRNA.
D. U5: associates with last nucleotide in one exon and the first nucleotide in the next exon.
E. U6: base pairs with 5′ end of the intron.
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #7
8. Which of the following complexes found in the spliceosome cycle is mismatched with its
snRNP(s)?
A. commitment complex: U6/U4
B. A complex: U1 and U2
C. B1 complex: all snRNPs
D. B2 complex: U2, U5 and U6
E. I complex: U2, U5 and U6
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #8
9. Which of the following splicing factors is required for the correct 3′ splice site selection?
A. SC35
B. Slu7
C. U11
D. SF2
E. SRp55
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #9
10. The catalytic center of the spliceosome appears to include
A. Mg2+.
B. U2 and U6 snRNP.
C. the branch point region of the intron.
D. Mg2+ and the branch point region of the intron.
E. Mg2+, U2, and U6 snRNP, and the branch point region of the intron.
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #10
11. Which of the following is the first snRNP to bind during the assembly stage of the spliceosome
cycle?
A. U1
B. U2
C. U4
D. U5
E. U6
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #11
12. The binding of which of the following snRNPs to spliceosome requires ATP?
A. U1
B. U2
C. U4
D. U5
E. U6
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #12
13. The expressed regions of genes are referred to as ________ while the noncoding regions are
called_______.
exons, introns
Blooms Level: 1. Remember
Section: 14.01
Weaver - Chapter 14 #13
14. The process by which introns are removed from immature mRNA is called _____________.
RNA splicing
Blooms Level: 1. Remember
Section: 14.01
Weaver - Chapter 14 #14
15. _______________ disrupting the normal splicing consensus sequence are one way to obtain
abnormal splicing patterns.
Mutations
Blooms Level: 2. Understand
Section: 14.01
Weaver - Chapter 14 #15
16. Joan Steitz was one of the researchers who used __________ studies to demonstrate that U6
snRNP binds to the 5′ end of the intron.
cross-linking
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #16
17. Some introns, such as Group II introns, are self splicing. These types of introns contain
catalytic RNA's, also referred to as __________.
ribozymes
Blooms Level: 1. Remember
Section: 14.03
Weaver - Chapter 14 #17
18. Exon definition involves the use of the ________ subunit of RNA polymerase II.
Rpb1
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #18
19. Alternative splicing is involved in _________ determination in fruit flys.
sex
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #19
20. There are a common set of ___________ Sm proteins that are specific to each snRNA.
seven
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #20
21. Introns are regions found in DNA sequences and mature mRNA sequences.
FALSE
Blooms Level: 2. Understand
Section: 14.01
Weaver - Chapter 14 #21
22. RNA polymerase II does not transcribe through introns, instead it jumps over them.
FALSE
Blooms Level: 2. Understand
Section: 14.01
Weaver - Chapter 14 #22
23. Heterogeneous nuclear RNA (hnRNA) is thought to be a precursor of mRNA.
TRUE
Blooms Level: 1. Remember
Section: 14.01
Weaver - Chapter 14 #23
24. Pre-mRNA splicing occurs in the cytoplasm.
FALSE
Blooms Level: 2. Understand
Section: 14.01
Weaver - Chapter 14 #24
25. Experimental evidence demonstrates that mRNA precursors are spliced via a lariat-shaped
intermediate.
TRUE
Blooms Level: 2. Understand
Section: 14.02
Weaver - Chapter 14 #25
26. Base pairing between the 5′ splice site and U1 snRNA is the only interaction essential for
splicing.
FALSE
Blooms Level: 2. Understand
Section: 14.02
Weaver - Chapter 14 #26
27. The spliceosome cycle is one way for a cell to regulate gene expression.
TRUE
Blooms Level: 2. Understand
Section: 14.02
Weaver - Chapter 14 #27
28. Immature mRNA splicing can be established by either exon definition or intron definition.
TRUE
Blooms Level: 2. Understand
Section: 14.02
Weaver - Chapter 14 #28
29. Alternative splicing patterns can lead to the production of different protein products from the
same gene.
TRUE
Blooms Level: 2. Understand
Section: 14.02
Weaver - Chapter 14 #29
30. SC35 is an RNA binding protein that is responsible for the commitment to splice the β-globin
pre-mRNA.
TRUE
Blooms Level: 1. Remember
Section: 14.02
Weaver - Chapter 14 #30
31. The discovery of alternate splicing helped prove the "one gene, one protein" theory.
FALSE
Blooms Level: 2. Understand
Section: 14.02
Weaver - Chapter 14 #31
14 Summary
Category # of Questions
Blooms Level: 1. Remember 18
Blooms Level: 2. Understand 12
Blooms Level: 5. Evaluate 1
Section: 14.01 11
Section: 14.02 19
Section: 14.03 1
Weaver - Chapter 14 31
