Content

List .................................................................................................................................................... 1
Chapter 1 Instrument specification ................................................................................................... 2
Chapter 2 Installation ........................................................................................................................ 9
Chapter 3 Performance and test flow .............................................................................................. 12
Chapter 4 Module Instruction ……………………………………………………………………..21
Chapter 5 Instrument Fluid Pipeline………………………………………………………………33
Chapter 6 Instrument Hardware Circuit…………………………………………………………..41
Chapter 7 Maintenance……………………………………………………………………………56
Chapter 8 Analysis Method………………………………………………………………………..72
Chapter 9 Troubleshooting List……………………………………………………………………84

1
 Chapter Ⅰ Instrument Specification

1.1 CS-T240 Model Specification

1.1.1 Product Composition：
The analytical part (host), operation part (computer system), the result output part, accessories and
consumables.
Product applicable scope: used for quantitative analysis of serum, plasma, urine, cerebrospinal
fluid and other clinical chemical constituents of sample.

2
1.1.2 Front ① ②

① Model Logo

② Upper Cover

③ Front Cover

1.1.3 Rear

① Purified Water Inlet

② Low Concentrate Waste Outlet

③ High Concentrate Waste Outlet

④ Detergent Inlet

⑤ Detergent Level Sensor Port

⑥ Left Back Cover

⑦ Syringe Pump

⑧ Right Back Cover

⑨ R S232 Port

① ②③ ④ ⑤ ⑥ ⑦ ⑧ ⑨

3
1.1.4 Working Table

① Reaction Cuvette Rinsing Unit

② Reaction Disk Unit ① ② ③ ④⑤ ⑥

③ Reaction Tank Liquid Level Detection

④ Stirring Unit

⑤ Sample &Reagent Adding Unit

⑥ Sample & Reagent Disk

⑦ Mixer Rinsing Tank

⑧ Probe Rinsing Tank

⑦ ⑧

1.1.5 Rightside

① Main Switch

② Power Socket

③ Fuse Installation Place

④ Main Power Indicator（Red）

⑤ Refrigeration Power Indicator（Green）

⑥ Analysis Unit Switch（exclude refrigeration power）

① ② ③ ④ ⑤ ⑥

4
1.2 Analysis Unit
The working speed of CS-T240 auto-chemistry analyzer is 240 tests / hour at constant speed
(single / double-reagent item), whose working period is 15 seconds. Instrument overall structure
adopts the project of "2-disks + 1-probe + 1-stirring rod"—one reaction disk, one sample &
reagent disk, one stirring rod, one sample & reagent probe used for adding reagent and sample,
one stirring rod used for mixing. "Grating + diode array" approach is adopted in optical
measurement unit for real-time optical collection of reaction cuvette. The 8-stop 12-step
automatically rinsing of the reaction cuvette is carried out during testing process.

1.2.1 Structure

1.2.2 Reaction Unit

Reaction Cuvette Reaction Disk

Reaction cuvette：120，optical path: 6mm

20×6 sets hard optical plastic cuvette
Incubation bath
Digital liquid sensor
8-stop 12-step rinsing of colorimetric cup

5
1.2.3 Probe and Stirring Unit
Stirring Unit Probe Unit
Probe unit：1

sample & reagent probe

High-precision digital liquid detector

Stirring Unit：1

High-speed hollow cup motor

Surface high-intensity Teflon coating

1.2.4 Control unit1

Circuit panel boxes: 5 panels ①

Order: from left to right
ISE control panel（Optional）
Sample reagent disk control panel ②
Rinsing & stirring control panel
Reaction disk control panel
Main control panel
②Switching Power Supply Box: 3
Order：form outside to inside
＋12V（lamp）
＋5V（digital circuit）±12V（simulation）
＋24V（motor, valve）
③ Circuit panel box power supply interface ③ ④ ⑤⑥⑦

④ halogen lamp power supply control interface（＋12V）

⑤ Cooling unit power interface（220V）
⑥ Fan power interface（220V）
⑦ Solid state relay panel interface（220V）

6
 ① ② ③ ④

1.2.5 Control Unit 2

Semiconductor refrigeration system：

①＋12V refrigerator power

②＋5V control panel power
③Fan（15W/220W）
④Control panel（with status indication）

7
1.3 Function Overview
Main work flow：
1. All mechanical moving parts initialization.
2. 3 times water blank measure is implemented after six time automatic rinsing
3. Sample reagent probe assimilates quantitive reagent when it descents to reagent sample disk
after the sample reagent disk rotates to designated R1 reagent position. And then, sample reagent
probe assimilates quantitive sample when it descents to reagent sample disk after the sample
reagent disk rotates to designated sample position.
4. After 8-stop 12-step rinsing, reaction cuvette stops at the sampling position, and sample reagent
probe rotates to reaction disk and descends to reaction cuvette to discharge the mix liquid(reagent
and sample), the reagent 1 and sample adding is finished.
5. Reaction cuvette is stirred immediately when it rotates to R1stirring position.
6. Sample＋R1 reagent react in reaction cuvette or temperatured.
7. If it is double reagent item test, sample reagent disk rotates to the designated R2 reagent
position and sample reagent probe descends to sample reagent disk to assimilate quantitive reagent
after a set period (6 mins).
8. The sample&reagent probe discharges R2 into reaction cuvette when it rotates to reaction disk.
9. Finishing R2 reagent adding, reaction cuvette is stirred after its one circle (R2 stirring position)
rotation.
10. Reaction cuvette carries out the collection of absorbance data when it passes the optical unit in
every period.
11.The reaction cuvette is rinsed automatically after reaction when passing the rinsing unit, and 15
minutes have been elapsed since sampling to rinsing.

Table 1-3-1 Main Function of Each Unit

Name Main function

Sample&reagent probe Execute sample, reagent assimilation and discharge of all
unit biochemical items and ISEitems
Sample&reagent disk Total 21 sample positions for carrying all test samples,
unit standard solution and control, 46 reagent positions for
carrying test reagent and detergent
Reaction disk unit Total 120 reaction cuvettes used as container of reaction
and colorimetry test.
Reagent stirring unit Stirring when reagent is added into reaction cuvette.
Optical Measure 12 wavelength absorbance by grating system
system groupware
Auto-rinsing unit Rinse reaction cuvette automatically by 8-stop 12-step
ISE unit （optional） Carry out ISE measurement (K、Na、Cl）
Barcode Total 1 for scanning reagent and sample bottles in sample
reagent disk

8
 Chapter 2 Installation

2.1 Space Requirement：

To make sure the space of maintenance, operation and repair, please follow the instruction as
below:
● Space between left (right) side of analyzer and the wall should ≥50cm
● Space between rear board of analyzer and the wall should ≥50cm
● Space in front of analyzer should≥100cm
● Make sure there is enough space for waste device and purified water equipment.

2.2 Power supply requirement：

● Power supply: AC220V±22V 50Hz/60Hz
● Power: 650VA
● Circuit breaker: 250V, 20A
A well grounded power supply socket is a must. Large electrical appliance such as air condition,
refrigerator, even cannot use the same socket with analyzer.
△! Warning:
Incorrect grounding may cause electric shock or instrument damage.
Input voltage should conform to requirement. 3KVA-line UPS power supply is advised.

2.3 Environment requirement

● Working temperature: 15℃～32℃
● Relative humidity: 32 ％～85％
● Atmospheric pressure: 76kPa～106kPa
● Environment should with no dust, vibration, major noise source and power interference
● Do not put the analyzer in the vicinity of brush motor, flicker fluorescent tube and other
constant on-off electrical equipment.
● Hard and flat ground is a must for the instrument.
● Avoid direct sunlight, do not put the analyzer in front of heat source and wind source
● Keep good ventilation.
△! warning：
Normal running and accurate result can not be guaranteed if instrument works beyond the
requirements mentioned above.

9
Please use air conditioner if the temperature or humidity can not meet the requirement above.
The heat generated during working process by the instrument will be emitted at the rear of the
instrument. Good ventilation should be kept well and ventilation equipment can be adopted if
necessary, but direct air current should be avoided, or inaccuracy of instrument test may be
caused.

2.4 Purified water equipment：

① water should be obtained from tap water pipe
② water conductivity should within 1uS/cm
③ water supply volume should reach 40L/h or more
④ The hydraulic pressure should within 49-343 Kpa

2.5 Instrument Installation Flow：

Make sure the installation place, space, electrical environment, installation room temperature and
purified water equipment can conform to requirements
Make sure instrument installation tools needed are complete and reagent and QC liquid are
enough.
Please check the prepared items according to packing list when open the package; please write
them down on the check report if any missing.
Place instrument in appropriate position, and mount with computer host, display and printer.
Connect water supply and waste liquid outlet equipment.
Infuse CS-anti-bacterial detergent into the 45th position of sample reagent disk.
Check whether the power and data wires are well connected
Install sample reagent probe and reaction cuvette
Check whether the sample reagent probe can move up and down flexibly

Get through the pure water machine, computer host, display and analytical unit power supply, and
enter CS auto-chemistry analyzer systematic application software. Initial user name: 001, initial
password: 001.
After enter software, follow the steps below in “Maintenance” interface.
（a）Injection pump exhaust
Execute injection pump exhaust to expel air in pipeline.
（b）Cleaning liquid pipeline exhaust
Executing irrigation detergent pipeline exhaust is infusing detergent into pipeline to expel air in
pipeline.
（c）Reagent&sample probe horizontal check
Make sure sample&reagent probe is right above reaction cuvette, rinsing groove and reagent
bottle.
Place two standard cups at outer circle position 43, inner circle 44 respectively in the sample&
reagent disk, and put two blank bottle in inner circle 2 and outer circle 1.Make sure the sample&
reagent probe is above reaction cuvette, rinsing groove, standard cup by implementing reagent
sample probe horizontal check.
（d）Stirring rod horizontal check
In order to make sure the stirring rod is above the reaction cup, rinsing groove.

10
（e）Mechanical movement check
Execute 20 times mechanical movement checks to make sure whether the washing block of
rinsing mechanism nozzle abrases the reaction cuvette or not and each mechanism runs normally
or not.
（f）Rinse reaction cup+ ISE
Select rinsing reaction cuvette in “Maintenance” interface, and execute rinsing reaction cuvette +
ISE if ISE equipment is collocated.
（g）Light quantity check
Light quantity result should be attached to installation check report with its value no more than
18000.
（h）Cup blank test
No.1 cup blank value should be within 18000, and 2-120 reaction cup check value should be
within 18000 ±800.

2.6 Clinical item test

Edit chemical parameters; register reagent info.; testing rate assay ALT, point assay, two-point
rate BUN; calculate the difference of parameter and the result of test should be attached to
installation check report.

2.7 Train Medical Personnel

2.8 Fill the Installation Check Report Detailedly.

11
 Chapter 3 Performance and Test Flow

3.1 Main Performance Index

3.1.1 Instrument standard specification

Performance Index Standard Specification

Grating rear spectrophotometry system,

simultaneous photometric processing of 12
Wavelength range
wavelength： 340 、380 、405 、450 、480 、
505 、546 、570 、600 、660 、700 、750nm

Wavelength
±2nm
precision

Reaction
37℃±0.1℃
temperature
Characteri
stics Simultaneously testing 60 colorimetric items
Test item
and 3 ISE items at most

Test
Rate assay ,end-point assay, 2-point assay.
method

Constant speed, 240 tests/ hour ( 360 tests/

hour
Test
speed speed with ISE)

12
 Sample & reagent disk,sample Reagent & sample disk with refrigerator,
position, reagent position semiconductor cooling system.Total 67
positions(21 routine samples, 45 reagent
positions “CS-bacterial phosphate-free
detergent”, 1 detergent position)

Serum, plasma, Urine, cerebrospinal fluid,

Sample type
ascites and other body fluids

Sample volume 3～35ul，0.1μl incremental

Test tube Φ（12－16）mm×（75－100）mm(±1

Test tube mm)

Reagent
sample
Standard cupΦ14mm×37mm(±1 mm)
system
Remaining sample volume More than 100μl

With liquid level detection and collision

Sample&reagent probe
detection function

Sample&reagent probe rinsing Inner, outer wall rinsing

Sample&reagent liquid level Digital liquid detecting, integration

sensor with sample&reagent probe

Reagent volume 10～350ul，1μl incremental

Reagent bottle 20mL 、70mL、100mL

Remaining reagent volume More than 3mL

Reagent storage temperature 5℃～15℃

13
 type：code 128

Size：

width: 8-12mm; alid length : within

40mm; start blank and finish blank:
within 3mm when cutting.
Barcode information
Sticking requirement:the lower edge of barcode
should be within 15mm-20mm away from the
test tube bottom to make sure right reading
barcode,and make sure the barcode is aligned
with sample position gap when putting the test
rack.

Reaction cuvette mode Discrete

Reaction cuvette
6mm
optical path

Reaction cuvette number 6 sets, 20 for each，total 120

Reaction time 15 mins

Reaction liquid volume 150～550ul

Reacti
on System
Light source 20W/12V Long-life quartz halogen lamp

Absorbance range 0～3.3ABS

QC QC interval, monthly QC

Automatically rinsing reaction cuvette, sample&

Automatic rinsing
reagent probe, stirring rod.

Stirring system separately stirring after adding

TCP/IP network port，standard RS-232 and USB

port
2.0 port
Data
Stylus printer, supporting the user-defined mode
system Printer
for report sheet

Connecting LIS/HIS system LIS/HIS system available

weight About 120Kg

Instrument
system
Dimensions 998*752*515 (length×width×height)

14
 power（VA） 650VA

Water consumption 5L/小时

Installation power 220V/230V，50Hz/60Hz，1000VA

requireme
Using environment Systems storage temperature : 0℃～40℃,
nt
volatility: <±2℃/H; storage humidity:30%RH～
80%R ， non-condensing ； at working,
temperature:15℃～30℃， volatility<±2℃/H；
atworking, relative humidity:35%RH ～
80%RH ，non-condensing ；not higher than
2000 meters above sea level.

3.1.2 Testing speed

Test conditions Degree of reduced ability to process (estimated)

Retest after sample

prediluted 80 tests/h（all tests after predilution ）

Use avoiding cross

contamination function 120 tests/h at least（reaction cuvette、sample reagent probe）

R1 and R2 items are

used simultaneously in 240 tests/h at least
testing

15
3.2 Test Flow

3.2.1 典型测试流程

16
3.2.2 Test Flow Instruction
1. Periodic movement sequence of sample reagent probe
a. Switch the pump to zero, internal and external wall rinsing
b.Inhale 3ul air.
c.Rotate to the position above the sample reagent disk
d.Descend till the sample&reagent probe point into liquid level about 2mm
e. Assimilate quantitive volume + push back redundant sample
f.Move to above the rinsing pool from the reagent bottle to rinse the external wall.
g. Inhale 3ul air
h. Move to above the sample&reagent disk
i. Assimilate quantitive volume sample
j.Move to above the reaction disk from sample cup or tube
k.Add sample and reagent into the reaction cuvette
l. Rotate to above the rinsing pool from reaction cuvette
→ (next periodic movement sequence).

2. Stirring rod periodic movement sequence：

a．Rotate to above reaction disk
b．Descend into reaction cuvette
c．Mix reaction liquid
d．Rise from reaction cuvette and rotate to rinsing bath
e．Descend into rinsing bath
f．Stirring rod rinsing
g．Rise from rinsing bath

3. Movement and time sequence of reaction disk

A track includes total 120 reaction cuvettes in reaction disk, and rotates in a fixed way when
testing. The reaction cup always rotates and stops 4 times counterclockwise, total 36＋4＋
82=122 （rotation and stop sequence 36－4－82）patches, in every working period, 15
seconds elapsed.

17
 120 个反应杯位置号

反应杯清洗机构

加试剂与加
反应盘复位点
样位置

搅拌位置

光电检测位

参考位置编号

figure 3-2 Position of reaction disk and probe

Outer circle figure: No. of reaction cuvette；inner circle figure: No. of mechanism position.

Reset point is in 71 position of reaction disk.

Reagent1、2 and sample probe is in NO.1 position, stirring is in No.3 position.

Reaction cuvette rinsing unit: NO.71、73、81 position

Reaction cuvette rinsing sequence:
1 → 3 → 5 → 7 → 9 → …… →117 → 119 （18 mins，60 times）
2 → 4 → 6 → 8 → 10 → …… → 118 → 120 （18 mins，60 times）

18
 i. Reaction Cuvette Rinsing Movement Sequence

C D
A B E F G H

Reaction disk rotate direction Test cell blank for 3 times（1 stop，2 pass）
figure 3-3 Reaction cuvette rinsing probe position

Above figure shows that 8 steps are needed when rinsing reaction cuvette. (3 times cell blank test
is added) , therefore, to finish rinsing one reaction cuvette, 12 steps are needed：
ii. Optical measurement movement sequence
Photometry in the entire process is adopted. In 13-minute reaction time, the continuous
determination of the absorbance of reaction solution is carried out. Reaction disk rotates 1 plus 2
pitches, about 15 seconds, absorbance values are measured out when the 120 reaction cups passing
optical axis of the photometer one by one.
Each reaction Cup in 3-minute reaction time was measured 12 times (12 photometric points),
4-minute reaction time was measured 16 times (16 photometric points), 5-minute reaction time
was measured 20 times (20 photometric points), 10-minute reaction time was measured 40 times
(40 photometric points), 13-minute reaction time was measured 49 times (49 photometric
points).
Light starting from the light source was focused by the lens, and passed the reaction cup first, and
then was disparted by concave grating. After spectrophotometry, each wavelength were received
by 12 fixed photoelectric sensors simultaneously, and were amplified 12 amplifiers, after Log
transformation to derive the rate of change of absorbance or absorbance. When dual-wavelength
testing is used, the concentration value is calculated by the difference of the main and
sub-wavelength absorbance or that of absorbance change rate, and therefore dual-wavelength
testing can not only compensate the blood lipid, hemolytic, jaundice sample test, but also
compensate on the result impacted by voltage changes, so that measurement is more accurate,
more stable.

19
20
 Chapter 4. Module Introduction

4.1 Sample&reagent probe unit

4.1.1 Function introduction
Sample&reagent probe can realize the assimilation from the sample test tube and reagent
bottle and add into reaction cuvette.

In addition, main function of reagent&sample probe component: liquid level detecting and
collision detection in movement process, probe block detecting function.

Other subsidiary function includes mechanical limit, power-down self-locking function.

Probe working position：

Reagent&sample probe component ： rinsing bath→sample&reagent disk assimilating

position→reaction disk/ISE sampling position；

Probe drive mechanism plays a key role of reagents and samples adding. The way of probe are
only up-down and circular moving, so two step motors are necessary to drive.

21
 4.1.2 Composition
Probe rotating arm
Probe rotating gear belt

Probe up-down light sensor block tablet

Probe rotating step motor

Probe up-down gear belt

Probe up-down step motor

Probe up-down weight

Figure 4-1 probe components configuration

Rotating fixed light sensor Rotating motor mounting

position hole

Up-down motor
mounting hole
Lower rotating axie centor

figure 4-2 Probe body and rotating body

Mandrel

Probe rotating gear

22
 Rotating probe drive ratio is 12:34, using 0.9° stepper motor and 8 segment controller. Control
accuracy can achieve 0.0398°.

Up-down main gear driver diameter is 19.1mm, the 60mm for the perimeter, also using 0.9°
stepper motor and 8 segment controller.Up-down control accuracy can achieve 0.01875mm.

4.2 Rotating mechanism unit

4.2.1 Function Introduction
The main function of rotating mechanism is bearing of the sample warehouse,reagent warehouse
and reaction disk, and drive it to rotate, so that sample and reagent carried in reaction cup
rotate to the designated location to finish sampling， mixing and other work.

4.2.2 Rotating mechanism composition

1, Reagent&sample turntable: The sample&reagent storehouse, disk rotating bracket, step
motor-driven components

2, The reaction disk turntable: reaction plate, the reaction cuvette, incubation bath, disk rotating
bracket, step motor-driven components

Mandrel Storehouse fixing

S/Driving seat

Zero light sensor

Sample&Reaction
Storehouse cover light sensor disk motor seat

Code disk light sensor The foot of bracket

4-4 Disk rotating bracket

23
 Driven gear
Drive gear belt

Code Driving step motor

disk and
light sensor

4-5 sample &reagent disk motor driver configuration

System transmission ratio is 10:1.Because of using 0.9 °step motor with 8 segment driver circuit,

the wheel rotation accuracy can achieve 0.01125°.

Leader of disk cover touch

switch

Connecting loop sample&reagent

disk rack
Connecting loop of
disk cover

4-6 Sample/reagent disk assembly configuration

Disk cover
45 teeth of sample&reagent switch light sensor
code disk

Original point block

and light
sensor

4-7 sample &reagent disk transmission code disk configuration

4.3 Refrigerator Unit

4.3.1 Function Introduction

The reagent&sample storehouse with refrigerator adopting semiconductor refrigeration, the

24
temperature maintains at 6 degrees -10 degrees, 21 sample positions and 46 reagent positions (the
45th fixed position for placing phosphor-free CS-anti-bacterial detergent)

4.3.2 Refrigeration system configuration and installation

Equipped
with
temperature-keeping
reagent
cooling storehouse

4-8 Reagent cooling storehouse and sample&reagent disk rack assembly configuration

Barcode reader scanning

window
Sample cup

Air inlet

Reagent kit

Air cooling fan

4-9 reagent cooling storehouse and sample&reagent disk rack assembly configuration

4.4 Reaction disk unit

Gear drive adopted reaction disk due to the relatively high positioning precision.

25
 Colorimetric cup Fixing pin
rotating rack

Driving gear

Driven gear

motor vibrator

4-10 reaction disk assembly configuration

120 colorimetric cup

120 teeth of code disk in
colorimetric cup position

4－11 reaction cup installation disk assembly configuration（1）

cuvette
cuvette install pin

Locking screw of
colorimetric cup rack

4－12 reaction cup installation disk assembly configuration（2）

26
 stirring rod rinsing
bath

Liquid level sensor

probe rinsing bath
rack

Spilling outlet of
Optical window incubation bath

Circular constant
Circular constant
temperature water
temperature water
outlet
inlet
4－13 Incubation bath components configuration

Phosphor-free
Incubation bath detergent inlet

4－14 reaction disk with incubation bath components assembly configuration

Optical system

4－15 reaction disk and optical system components assembly configuration

27
4.4 Stirring Unit

Stir and mix reagent and sample after adding them。

Rotating mechanism of stirring and rotating arm adopts the direct drive way of step motor output
axle, using 8 segment drive circuit of 0.9 ° motor, and the controlaccuracy can achieve 0.1125 °.
The largest angle is limited by the open angle of rotatation code disk mechanical limit. And
positioning is determined separately by the left and right light sensors.

Mechanism rotation
mechanical limit

Rotation code disk

Stirring unit swing
and light sensor
motor

Stirring mechanism
up-down motor

Stirring mechanism
up-down slider

Stirring mechanism
up-down 4－16 Stirring components configuration
light
sensor

Stirring mechanism
up-down motor
Up-down slider axle

4－17 stirring up-down driver configuration

Curve axle and curve handle drive is adopted by stirring up-down mechanism .

28
Up-down linear
sliding axle

Up-down
Up-down slider
guide axle

4－18 stirring up-down slider drive configuration

Because of mixing body movements are used by way of the crankshaft crank, so the

movements of the positioning accuracy at different locations different. Landing and taking-off

between the location of the speed of the highest, lowest accuracy, and precision at both ends of the

highest, the lowest speed.

Electrical axis from the axis of the slider bearings the size is 16.5mm, crank in horizontal position,

the landing position accuracy is 0.032mm.

Due to curve axle and curve handle drive is adopted by stirring up-down mechanism, the

up-down positioning precision are different at different position. The speed is the highest and

precision is lowest at the middle, however the precision is highest and speed is lowest at the two

ends.

The size of Motor axis from the axis of the slider bearings is 16.5mm, when curve handle locates

horizontally; the up-down position precision is 0.032mm.

29
 Up-down slider
Up-down slider

4－19 stirring mechanism up and down positions

30
4.6 Colorimetric cup rinsing mechanism

Rinsing probe Colorimetric

rack of cup rinsing
colorimetric cup probe

Up-down
slider
Up-down step motor

4－20

Only one-dimensional movementavailable to colorimetric cup rinsing mechanism,and driving

mechanism adopts the up-down driving mechanism of stirring rod.

4.7 Rack
Rack includes the main rack, electrical, gas liquid valve and water tanks and other racks.

Bottom-Board Main working table board

Isolating
standing
board

Adjusting foot

4－21 Main rack configuration

31
Electrical control
refrigeration controller
box

Switch

Switch power

4－22

32
 Chapter 5. Instrument Fluid Pipeline

5.1 Main function of liquid line

The CS-T240 liquid line system can be divided into four parts: water inlet tank, 37 degrees
centigrade temperature, colorimetric cup cleaning, the inner and outer arms and stirring rod
cleaning.

1. CS-T240 liquid line system includes sampling subsystem and cleaning subsystem.

2. Sampling subsystem uses one probe plus one stirring rod and one injector. The injector use
500uL

3. The inner and outer wall cleaning of probe and stirring rod uses barotropic driving.

4.Cleaning bath: two cleaning bathes plus waste liquor in the reagent storehouse, together
with the flooding waste liquor in the reaction disk etc.
5. The reaction cuvette cleaning uses the way named 8-stop 12-step.

6.Water supply: uses the special outboard water-supply equipment and the special
water-supplying machine.

7. Waste liquor: reticulate pattern pipe as low concentrate waste pipe with inside diameter of
12mm, wall thickness 3mm, high concentrate waste pipe is also reticulate pattern pipe with inside
diameter is 8mm and wall thickness is 2mm

8. Source of power: the power of cleaning comes from the magnetic pump..

9. The cleaning of reaction cup should use one detergent..

10. The vacuum degree for vacuum pump assimilating≥ -70kPa

11. The inner and outer wall cleaning of probe uses independent solenoid valve while the
stirring rod use one solenoid valve.

33
5.2 Liquid Line Principle
5.2.1 CS－T240 Liquid line principle sketch map

34
5.2.1 liquid line of water inlet tank

Explanation：
Z1——heater； Z2——lamp coller； Z3——float； Z4——temperature sensor；
Z5——water tank； Z6——magnetic pump； Z7——water seven-pass；Z8——air discharger
Z9——flow controller；Z10——pressure sensor；Z11——5 valve plate assembly；A——detergent；
B——probe inner wall rinsing；C——Cuvette rinsing；D——stirring rod rinsing；E——probe external
wall rinsing ； F——water inlet ； G——incubation bath inlet ； H——air outlet ；
M——overflow outlet； N——cuvette rinsing

1, When the low water level float detects out the signal, open the inlet valve SV13, and water tank
begins to be infused water until the high water level float detects out signal, then turn off SV13 to stop
the water. Influent flow is as follows:

Stop
Alarm of low influent Alarm of high water influent
water level check water level check water

2, When the low water level float did not detect out signal, the water tank heater began to work,and
temperature control started. Magnetic pump began to work simultaneously.
3, Output water pressure of water tank is controlled by the magnetic pump and fixed damper regulator.
Magnetic pump head is 4.6 m（50Hz）. Control water pressure is around 0.45kgf/cm2.
4,The tank water is pumped into the seven-pass through magnetic pump: the first pass to the

35
five-valve plate assembly, used for cuvette rinsing, stirring rinsing and external wall rinsing of the probe;
second pass through the degassing device for the internal wall rinsing of the probe; third pass with the
detergent used for the first probe rinsing of rinsing unit; fourth pass for the incubation bath; fifth pass
access to pressure sensor for detecting the tank pressure; sixth pass for regulating the water tank
pressure.
5, When abnormality occurs to the float, possibly water tank remains the status of inputting water. When
the water tank is full of water, SV13 not shut down, tank outflow water spills out from the overflow pipe
into the waste liquid pipe.

5.2.2 Constant temperature system liquid line

This system offers precise constant 37 degrees water to the incubation bath of reaction disk, and cools
the high temperature light source simultaneously. This system consists of magnetic pump, incubation
bath water inlet valve, incubation bath water outlet valve, liquid level detector and temperature
controller consists of the heater, temperature detector and the shell.

Explanation：Z1——incubation bath； Z2——liquid level detector； Z3——halogen lamp； Z4

——flow controller；Z5——lamp cooler；Z6——constant temperature unit； Z7——block； Z8
——magnetic pump；Z9——flow controller； A——incubation bath water outlet； B——incubation
bath water inlet；C——overflow outlet

1, Open the inlet valve SV6 and turn off outlet valve SV10 to infuse water into incubation bath,
simultaneously with reagent&sample probe adding phosphor-free anti-bacterial rinsing liquid to the
incubation bath, liquid level detector determining whether to stop water.
2, Turn off outlet valve and inlet valve, and start the water circulation magnetic pump and temperature

36
controller. In order to improve the adjusting performance of the PID temperature controller in high
temperature environment, the system is added the cooling device through the water tank to get a
small amount of temperature cooled.
3, Turn off magnetic pump and temperature controller, open the drain valve SV10, time to turn off the
drain valve when the incubation bath is draining.
5.2.3 Probe internal and external wall rinsing

1,Open valve SV9 can get the inner wall of reagent sample probe rinsed. Probe position should be at
the top of the corresponding cleaning trough when cleaning so that waste liquid can get out of the
instrument.
2,Open the valves SV4 and SV5 to rinse the external wall of sample&reagent probe and stirring rod.
Fixing pressure adjusting piston is adopted to every external wall rinsing pipeline to avoid rinsing water
spilling out of rinsing bath.

Explanation：Z1——syringe pump unit；Z2——sample reagent probe；Z3——probe rinsing bath；Z4

——stirring rob rinsing bath；Z5——five-valve plate unit；A——water tank；B——waste outlet

5.2.4 Colorimetric cup rinsing liquid line

In order to achieve the cleaning effectively, colorimetric cup rinsing adopts warm water. In order to

improve cleaning speed, colorimetric cup adopts hydraulic valve switch and vacuum liquid exhaust.

1, warm water provided by heating tank of inlet water is about 34 ℃. Water pressure is produced by

37
magnetic pump from the water tank and adjusted by voltage regulator to the stable pressure, about

0.45kgf/cm2.

2, vacuum pump, vacuum tank and pressure detector composes vacuum source with pressure value about

-0.7kgf/cm2. Vacuum tank has the function of stable vacuum pressure.

3,Vacuum liquid discharging is high concentrate liquid – reaction liquid including concentrated patient

sample and reagent,requiring separate collection.

4, when the colorimetric cup cleaning mechanism descends, turn off valves open the valve SV11. It tarts

assimilating sample under the vacuum pressure, and the liquid of colorimetric cup will be discharged

after a short period of time when the rinsing mechanism arrives at the bottom of Colorimetric Cup.

5, cleaning liquid and ionized water adding is completed by the SV1、SV2、SV3、SV7 and SV8 which

are timed. Because the vacuum liquid discharging begins to work simultaneously when adding liquid to

discharge redundant liquid, the liquid will not spill outside colorimetric cup.

6, SV7 and SV8 valves are responsible for adding cleaning liquid.

SV8 valve is three links, NO port connect with vacuum tank, NC connect with water seven pass.Before

adding or after the previous adding, open the valve SV7, but valve SV8 is at COM and NO conduction

status (power off status). Because there is a one-way valve in the 3-way top pipeline, cleaning fluid

flows into the middle pipeline of the SV7 and SV8 valves, and time valve SV7, cleaning liquid will

remain in pipeline. Open valve SV8 when adding, cleaning liquid will be added into colorimetric cup

through one- way valve under the pressure. About 500ul cleaning liquid is consumed each time.

38
Explanation：Z1——cuvette rinsing unit；Z2——detergent component；Z3——five-valve plate unit；

Z4——vacuum pump unit；Z5——liquid collection vaccum tank unit；Z6——nozzle1；Z7——nozzle

2；Z8——nozzle 3；Z9——nozzle 4；A——water tank；B——iquid collection vaccum tank；C——

high concentrate waste outlet

5.2.5 ISE Part

39
40
 Chapter 6 Instrument Hardware Circuit

6.1 Hardware configuration

PC

main control board（including

the control of AC ，pressure
and temperature monitoring）

Reaction Sample Stirring ISE

Rinsing A/D
module module module module
collection
A/D

ISE A/D collection

amplification
Photoelectricity conversion and
Reaction disk

Sampling mechanism

Rinsing unit
Stirring unit

ISE pump sets

ISE valve sets
Sample disk

convertion
and

Figure 6-1 Hardware main frame

41
6.2 Security Note:
At working, touching hardware panel with hand or any other objects is forbidden.

In order to dismount panels, operation is only allowed when cut off power (220V, AC).

6.3 Circuit board and function list

PCB Name Function Description Circuit
board No.
1. Carrying on the communications between upper and lower
machine, and that with cooling board.
Main control
2. Magnetic valve control：SV6、SV10、SV13

board 3. Monitoring of high and low temperature water tanks

4. Monitoring of incubation bath and vacuum tank liquid level
5. AD board data processing
6. Solid-state relay board control
Reaction disk 1. Communications with the main control board

2. The reaction disk rotation mechanism control

Circuit board
Reagent&sample disk 1. Communication with the main control board
circuit board
2. Sample&reagent probe mechanism control
3. Sample&reagent disk control
4. Injection pump control

5. Magnetic valve control: SV5、SV9

Stirring board rinsing 1. Communication with the main control board
2. Stirring mechanism control
3. Rinsing mechanism control

4. Magnetic valve control: SV1、SV2、SV3、SV4、SV7、

SV8、SV11
AD board 12 AD-wavelength data collection

42
ISE 1. Communication with the main control board
2. ISE pump motor control
Circuit board
4. Magnetic valve control: ISV1、ISV2、ISV3、ISV4

5. ISE preamp board data collection

1. 37℃constant temperature heater control（200W）

2. Water tank heater control（200W）

Solid relay board
3. Gear pump control
4. Water circulation pump
7. Halogen lamp control
refrigeration board 1.Semiconductor refrigeration control and temperature display
2.Semiconductor current monitoring and current value display
3. Fan control
Level detecting board 1. Sample reagent probe liquid level detection
2. Incubation bath liquid level detection
3. Alkaline detergent liquid level detection
4. Vacuum liquid level detection

ISE Preamp board K、Na、Cl electrode preamp

Mother board 1. Providing reaction disk board, sample reagent board, rinsing
stirring board, main control board, ISE board with connection
and power supply.

2. Communication among circuit panel

6.4 Instrument electrical principle wiring and function

6.4.1 Communication system and switch power

1. Wiring

43
2. Function
As shown in above figure:
Power input: ~220V
L1、N1: AC power filter S1: power switch
G1: power filter connecting with the ground of bottom-board
P131: AC port of solid state relay board
J501: fan port of AC system J502: AC power port of cooling system
J503: power Supply Port of Halogen lamp J504: Zero line port
J505: power switch of circuit board box J506: power port of solid state relay board
N1:±12V, 5V switch power, power supply for the mother board of circuit board

N2: 12V switch power，power supply for halogen lamp

N4: 24V switch power，power supply for step motor

44
6.4.2 Refrigeration system

1. Wiring

2. Function
Refrigeration system is composed of semiconductor refrigeration module, radiator, fan.
The main function of refrigeration board is controlling the semiconductor refrigeration module, to
keep the reagent disk temperature within 6-10℃.
As shown in the figure :
U1：refrigeration control board is the CPU of refrigeration system. It controls the working of
refrigeration system on the basis of the reagent disk temperature detected by temperature sensor.
Semiconductor refrigeration module and fan are controllable. The refrigeration control board starts
when the temperature beyond 6-10℃ to adjust the temperature. It also communicates with the main
control board.
DS18B20：the sensor used to detect the reagent disk and environment temperature
D1－D2：semiconductor cooling module（Peltier）
N3：12V switch power
N2：5V switch power

6.4.3 Main control board

45
1.Wiring

46
2.Function
Communicate with PC through serial port to transmit data, command and alarm information.

Transmit data and command with reaction disk board, sample&reagent disk board, ISE board,
refrigeration board and AD board communication through mother board.

As shown in figure:

Solenoid Valve:

Refer to 5.2.1

SV6 －water inlet valve of incubation bath

SV10－ water outlet valve of incubation bath

SV13－water inlet valve of water tank

J01：control port of vacuum pump, valve.

J02：communication port with computer（COM port）

J03：AD board data collection port

J04：pressure sensor of water tank, incubation bath.

J05：temperature sensor of water tank, incubation bath.

J06：solid state relay board port .

J07：float and liquid level signal interface.

J10：serial port monitor communication port.

J705：optocoupler signal interface of reaction disk.

47
6.4.4 Solid state relay board
1. Wiring

2. Function
Its main function is to control the solid state relay through main board to turn on and turn off the pump ，
motor, heater and lamp.

As shown in figure ：

Heater 200WA：water heater of incubation bath

WATER TANK PUMP：water circulation pump of water tank

GEAR PUMP：gear pump

FLUME PUMP：water circulation pump of incubation bath

LAMP：Halogen lamp

48
6.4.5 Circuit board of reaction disk
1. Wiring

2. Function
The main function of circuit board of reaction disk is to receive the command from the main control
board, communicate with the main control board, transmit data and alarm command.
Controlling sample&reagent unit includes sample&reagent disk rotation, probe unit running.
Barcode scanning of sample&reagent disk
J702：motor drive port of reaction disk
J704：signal receiving port of reaction disk clear optocoupler, count optocoupler. J705：output port of
reaction disk count optocoupler
J706：output control signal port with AD board

49
6.4.6 Rinsing mixing circuit board
1. Wiring

50
2. Function
The main function of rinsing mixing circuit board is to receive the command from the main control
board, communicate with the main control board, transmit data and alarm command.
Controlling sample&reagent unit includes sample&reagent disk rotation, probe unit running.
Barcode scanning of sample&reagent disk
As shown in figure：
J041: signal port of rinsing mixing optocoupler
J042: drive port of solenoid valve
J045: stirring motor control port.
J048: communication port with cooling board
Solenoid valve：refer to 5.2.1
SV1：cuvette rinsing probe（the second one）control solenoid valve
SV2：cuvette rinsing probe（the third one）control solenoid valve
SV3：cuvette rinsing probe（the fourth one）control solenoid valve
SV4：stirring rod rinsing control solenoid valve
SV7，SV8：cuvette rinsing probe（the first one）control solenoid valve
SV11：cuvette vacuum control solenoid valve

51
6.4.7 Circuit board of sample&reagent disk
1. Wiring

52
2. Function
Receive the command from the main control board, communicate with the main control board, transmit
data and alarm command.
Controlling sample&reagent unit includes sample&reagent disk rotation, probe unit running.
Barcode scanning of sample&reagent disk
As shown in figure ：
J041: signal port of sample&reagent disk optocoupler
J042: signal inlet port of probe position limit optocoupler and liquid level detection optocoupler
J043: signal inlet port of injection pump optocoupler
J044: SV5,SV9 drive signal port of solenoid valve
J045: motor drive port of sample&reagent disk
J046: motor drive port of injection pump
J047: motor drive port of probe
J048: communication port of barcode scanning

53
6.4.8 ISE circuit board
1. Wiring

2. Function

54
The instrument add calibrator into the reaction cuvette.The SIP injection pump aspirate the calibrator,
and pump it into the Na、K、Cl electrode pipeline of the reaction tank. Test the electric potential on the
basis of reference electrode. The sample probe aspirates sample and diluent, and pump it into the
reaction cuvette. Test the electric potential, and the concentration can be calculated.
J2：optocoupler port of aspirate nozzle elevator mechanism
J5：motor port of proportioning pump
P13：optocoupler port of proportioning pump
J61：motor port of aspirate nozzle elevator mechanism
J62：solenoid valve port
J64：preamplifier board port
ISE preamplifier board：amplify electrode signal

55
 Chapter 7 Maintenance

In order to ensure reliable system performance, excellent working status and span, please conduct
system operation and regular maintenance strictly in accordance with the requirements in the service
manual. Learning maintenance and overhaul of this chapter is also very important and in-depth study
will enable the instrument to achieve the best running status and exert the best performance.

Warning：

Do not carry out maintenance this chapter doesn’t mention. Otherwise,

it could lead to system damage and personal injury.

Do not touch any other parts except user self-operation and maintenance
which are clear recorded.

Unauthorized repair of the system may lead to system damage and

personal injury, and commitment term of the repair contract is no longer
valid.

Upon completion of maintenance work, make sure the system is

working normally.

Do not splash water, reagent and other liquid onto the system's
mechanical or electrical parts.

Biological contamination danger ：

In the process of maintenance work, be sure to wear gloves, put on work

clothes to prevent them from being infected and, if necessary, wear
protective glasses.

56
7.1 Maintenace preparation
Tools, high concentrate detergent and alcohol maybe used in maintenance.

1.Tools
 One set of hexagon wrench
 Cruciform Screwdriver (large, medium and small)
 Injection needle hose
 Small tweezers
 Clean gauze

2. High concentrate detergent

 Acid detergent，0.1mol/L hydrochloric acid
 Alkaline detergent，0.5％(V/V) sodium hypochlorite

Warning：
Acidic high concentrate detergent and alkaline high concentrate detergent
mixed generate poisonous gas. Do not mix them.

Caution：
Following high concentrate detergent designated by Dirui：
Acidic high concentrate detergent: 0.1mol / l hydrochloric acid; alkaline
high concentrate detergent: 0.5% (V / V) sodium hypochlorite.
Please use the high concentrate detergent designated by Dirui. If
undesignated types of high concentrate detergent are used, inappropriate
analysis results might be received.
Dirui recommends the use of alternating acidic and alkaline high
concentrate detergent, for example, use acidic high concentrate detergent
after power on, then use alkaline high concentrate detergent next time
after power on.

7.2 Daily maintenance

7.2.1 Check injection pump
The purpose of checking the injection pump is check whether leakage exists.

1 Make sure that Power of analysis part has been switched off.

57
 2 The injection pump can be seen in figure.

3 Observe whether the injection pump is leaking.

If so, check the leakage causes, and check the pipeline and connector timely.

7.2.2 Check/rinse sample & reagent probe

1 In online status, click “Instrument resetting” in “Maintenance”, and instrument
executes resetting.

2 When cleaning samle&reagent probe, carefully observe whether the outflow of

sample&reagent probe internal wall is continuous, whether the direction of flow
is consistent with the sample&reagent probe and the outflow of external wall is
continuous, and whether water volume is normal.
If not normal, clean sample&reagent probe(refer to 7.3.1)
If still not normal, check the corresponding liquid line channel; check whether
water supply of water tank and water pressure is normal.

7.2.3 Rinsing stirring rod

1 In online status, click “Instrument resetting” in “Maintenance”, and instrument
executes resetting.

2 When cleaning, carefully observe whether the stirring rod works normally, If not
normal, check the corresponding liquid line channel, check whether water supply
of water tank and water pressure is normal.

7.2.4 Rinse rinsing mechanism

1 In online status, click “Rinse reaction cuvette” in “Maintenance”, and instrument
executes reaction cuvette rinsing.

58
 2 When rinsing, carefully observe rinsing probe working and whether probe infusing
is normal and assimilating is completely.
If infusing abnormal, check pressure value of water infusing pressure gauge
If assimilating abnormal, check pressure value of assimilating vacuum
7.2.5 Check waste connection and discharging

1
Biological contamination danger:
During waste water operation, please put on gloves, put on work
clothes and if necessary, wear protective glasses.
Check whether liquid waste disposal system is normal every day, and maintain
waste liquid pipe is not bent and discharges smoothly and high and low
concentration waste liquid are disposed properly (refer to local standards of
dispose waste liquid).

7.2.6 Rinse instrument surface

Check whether liquid waste disposal system is normal every day, and
1
maintain waste liquid pipe is not bent and discharges smoothly and high
and low concentration waste liquid are disposed properly (refer to local
standards of dispose waste liquid).

7.2.7 Check printer and printing paper

1 Check printer power supply indicator, preparation indicator and printing paper
daily.

7.3 Weekly maintenance

7.3.1 Rinse sample reagent probe

Warning:
Please be careful to avoid hands from being scratched
Biological contamination danger:

In operation, please put on gloves, work cloths, and put on protective glasses
for the best.

Do not dispose the gauze used to clean sample probe at your own will, please
follow the relevant provisions for proper disposal.

59
1 Make sure the analysis part power supply is switched off.
2 Lift the rotating arms of samle&reagent probe by hands to the top position, and
rotate it to the top of sample&reagent storehouse for convenient operation.

3 Caution:
When cleaning, do not touch directly the probe surface to
prevent probe scratch; avoid too much hand force to prevent
deformation of the samle&reagent probe.
Note:
Acidic and alkaline detergent can be used alternatively, for
instance, acidic detergent is used at previous time
maintenance, use alkaline detergent at this time maintenance.
Wipe the external walls of sample&reagent probe lightly with cotton
stick moisturized with alcohol, especially the point of probe, until no
impurities left at all.

4 Wipe sample&reagent probe with the gauze dipped with deionized water.
5 After cleaning, lift the rotating arms of sample&reagent probe to the top
position, and rotate the rotating arm of sample&reagent probe to locate the
sample&reagent probe above the rinsing bath of sample&reagent probe.
Caution:
After cleaning the surface of a sample&reagent probe, please
make sure sample&reagent probe must be rotated to the top
of sample&reagent probe rinsing bath.

60
6 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample&reagent probe and
rinse them with deionized water.

7.3.2 Rinse stirring rod

Biological contamination danger:

In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean stirring rod at your own will,
please follow the relevant provisions for proper disposal.
1 Make sure the analysis part power supply is switched off.

2 Lift the stirring rod by hands to the top position, and rotate its rotating arm to a
position for convenient operation.
Caution:
When cleaning, do not touch the stirring rod surface directly to
prevent scratch; avoid too much hand force to prevent deformation
of the sample&reagent probe.
Note:
Acidic and alkaline detergent can be used alternatively, for instance,
acidic detergent is used at previous time maintenance, use alkaline
detergent at this time maintenance.
Wipe the surface of stirring rod lightly with cotton stick moisturized with
alcohol, especially the point of probe, until no impurities left at all.

4 Wipe stirring rod with the gauze dipped with deionized water
5 After cleaning, lift the rotating arm of stirring rod to the top position, and rotate
the rotating arm of stirring rod to locate the stirring rod to the top of the rinsing
bath.

61
 6 Switch on the power of analysis part and wait 30 seconds, enter the "maintenance
- routine maintenance" column to implement “instrument resetting", the system
will automatically reset the sample&reagent probe and rinse them with deionized
water.

7.3.3 Sample/ barcode window rinsing

Caution:
Do not gaze scanning laser light, or it may cause eyes injury

1 Make sure the analysis part power is switched off.

2 Remove the reagent sample disk cover, and then remove the sample&reagent disk.
3 Wipe the scanning glass window lightly with gauze dipped with deionized water.
4 Remount the sample&reagent disk and cover them.
5 Switch on the analysis part and wait 30 seconds, the system will reset
automatically.

7.3.4 Rinse reaction cuvette

The contamination of sample&reagent probe, stirring rod and reaction cuvette will affect the
accuracy of measurement. The reaction cuvette requires intensive rinsing.

1 Place 70 ml detergent (1mol of hydrochloric acid or 0.5% NaOH solution, use

the two types of solution alternatively, one week one solution) at the 45th
detergent position of sample&reagent disk.

2 Click “Maintenance” functional key to enter “System maintenance” menu,

and select “Rinse reaction cuvette” to execute.

7.4 Monthly maintenance

7.4.1 Rinse sample&reagent probe rinsing bath

Warning:
Please be careful to avoid being scratched by probe.

62
 Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample&reagent
probe.rinsing bath at your own will, please follow the relevant
provisions for proper disposal.

1 Make sure the analysis part power is switched off.

2 Lift the rotating arm of sample&reagent probe by hands to the its top position, and
rotate its rotating arm to keep reagent&sample probe away from rinsing bath for
convenient operation.
3 Clean the inside and appearance of sample&reagent probe rinsing bath with clean
cotton stick.
4 After cleaning, lift the rotating arm of sample&reagent probe to the top position,
and rotate the rotating arm of sample&reagent probe to locate the probe to the top
of the rinsing bath.

Caution：
After the work of sample&reagent probe surface rinsing,
please make sure to rotate reagent&sample probe to the top
of reagent&sample probe rinsing bath.

5 Switch on the power of analysis part and wait 30 seconds, enter the "maintenance -
routine maintenance" column to implement “instrument resetting", the system will
automatically reset the sample&reagent probe.

7.4.2 Rinse stirring rod rinsing bath

Warning:
Please be careful to avoid being scratched.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean stirring rod at your own
will, please follow the relevant provisions for proper disposal.

1 Make sure the analysis part power is switched off.

2 Lift the stirring arm to the top position by hand to one side of rinsing bath.
3 Wipe stirring rod with clean soft gauze.

63
7.5 Every 6 months maintenance
7.5.1 Check light source lamp
Lamp light source of optical system will gradually be aged in use, and will cause an increase in noise
during measurement. If the cuvette blank and light source intensity attenuation is out of range or the
working time of light source lamp accumulates over 2000 hours, the light source lamp should be
checked.

Caution：

Please use consumables recommended by DIRUI company, using other

consumables may cause system performance degradation.

Do not touch the light source lamp shell surface and lens in front of
the light source lamp by hand, because it may change the characteristics
of the light source. If you accidentally make noodle stained with filth,
absorbent cotton dipped by absolute alcohol can be used to clean it.

1 Turn off the system main power, so that the light source box and light source
lamp will be cooled for at least 15 minutes.

Warning:

High-temperature light source lamp and light box will cause

burn. Operation is carried out only after the light source and light
source lamp are cooled.

2 Loosen the fixing screws of rinsing mechanism; remove the rinsing mechanism
of reaction cuvette. Loosen the setscrews of reaction disk and remove the
reaction disk. Place the reaction cuvette at dry and clean position. Loosen the
two fixing wire connecting poles of halogen and remove down-lead.

64
 3 Loosen the two screws fixing light source seat to remove halogen lamp.

4 Mount a new halogen lamp according to the above opposite steps; pay attention to
tighten the screws. The cooling rubber hose in the lamp room can not be twisted and
down-lead can not be loosed or cocked.

5 Remount the reaction disk, the reaction cuvette and rinsing mechanism; switch on
the power supply of analysis part. After standby mode, single-click “Next” in
“System maintenance” window; infuse purified water into reaction groove. After
instrument standby mode, execute light quantity check function. Check the back of
halogen lamp if the light quantity conforms to the requirement to start test.

7.6 Every year maintenance

7.6.1 Check water of cooling system
7.7 Irregular check
7.7.1 Rinse sample reagent probe
If the water flow is not normal when rinsing sample&reagent probe, sample&reagent probe,the probe
may have been blocked and cleaning is needed to reagent&sample probe.

Warning:
Please be careful to avoid being scratched.

Biological contamination danger:

In operation, please put on gloves, work cloths, and put on
protective glasses for the best.

65
1 Make sure the analysis part power is switched off.

2 Remove sample&reagent disk cover and then sample&reagent disk

3 Lift the rotating arm of sample&reagent probe by hands to its top position, and
rotate its rotating arm to keep sample&reagent probe away from rinsing bath for
convenient operation.
4 Hold shell claw of probe rotating arm with fingers and lift to remove.

5
6 Loosen pipeline interface

7 warning：
Carefully place dismounted sample reagent&probe and prevent it
scratching human body and sample&reagent probe damage.
Note：
Take out sample reagent&probe from the rotating arm and be
careful to operate to avoid the damage of probe point caused by
touching rotating arm.

66
 Note：
Sample&reagent probe is precisely processed to ensure the sample and
reagent adding precision. If the probe point is damaged or bent, checking
sample&reagent probe is a must, or no guarantee can be made for test
precision.

7.7.2 Clean sample&reagent probe

Warning：
Please be careful to avoid being scratched by probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective glasses
for the best.
Do not dispose the gauze used to clean sample&reagent probe at your own
will, please follow the relevant provisions for proper disposal.

1 Put a stainless steel wire through the sample&reagent probe point to clean the
impurity in the probe.

Caution：
Sample&reagent probe is precisely processed to ensure the sample and
reagent adding precision. If the probe point is damaged or bent, checking
reagent&sample probe is a must, or no guarantee can be made for test
precision.

7.7.3 Install sample&reagent probe

Warning：
Please be careful to avoid being scratched by probe.

Biological contamination danger:

In operation, please put on gloves, work cloths, and put on protective
glasses for the best

Dismounting sequence is opposite to that of sample&reagent probe.

67
 Caution：
Sample&reagent probe is precisely processed to ensure the sample and
reagent adding precision. If the probe point is damaged or bent, checking
sample reagent probe is a must, or no guarantee can be made for test
precision.

7.7.4 Rinse sample&reagent probe

When it is found that the water level of rinsing bath is too high when rinsing sample&reagent probe
because of no discharging available, which may be caused by the blocked leaking hole. Cleaning sample
&reagent rinsing bath is necessary.

Warning：

Please be careful to avoid being scratched by probe.

Biological contamination danger:

In operation, please put on gloves, work cloths, and put on protective glasses
for the best

1 Make sure the analysis part power is switched off.

2 Lift the rotating arm of sample&reagent probe by hands to its top position,
and rotate its rotating arm to keep sample&reagent probe away from rinsing
bath for convenient operation.

3 Infuse about 1ml alkaline detergent of 0.5% (V / V) sodium hypochlorite or 84

disinfectant into rinsing bath for 10 minutes.

4 Switch on the power supply of analysis part

5 Lift the rotating arm of sample&reagent probe by hands to its top position,
and rotate its rotating arm to keep sample&reagent probe above the rinsing
bath of sample&reagent probe.

Caution：
Please rotate the sample&reagent probe to the top of sample&
reagent probe rinsing bath after clean rinsing bath of sample
&reagent probe.

6 Select and execute “Instrument resetting” after enter “Maintenance-routine

maintenance”, and the system will reset sample &reagent probe and rinsing
bath will be rinsed with deionized water automatically. Observe the outflow of
reagent&sample probe rinsing bath.

7.7.5 Rinse stirring rod

68
If the stirring rod is damaged, please check stirring rod in accordance with following steps strictly.

Warning：

Please be careful to avoid being scratched by probe.

Any touch is forbidden except at the knurling of it only by hand when

checking, and prevent any scratch on the flat part of stirring part.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective glasses
for the best
Please deal with removed stirring rod properly.
Caution：
Please use consumables recommended by DIRUI company, using other
consumables may cause system performance degradation.

1 Make sure the analysis part power is switched off.

2 Prepare a new stirring rod and wipe the flat part of it with gauze or cotton stick
dipped with cleaning liquid, and then wipe it with gauze dipped with deionized
water.
3 Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm for convenient operation.
4
Caution：
When pulling out stirring rod, make sure the direction of force is
vertical to that of axis of rotating arm. Lateral force may damage
the axis or stirring rod.
Remove stirring rod after loosen the two fixing screws.

5 Prepare a new stirring rod, and wipe the front of the stirring rod with gauze
dipped with 2% -antibacterial detergent.

69
6 When mounting new stirring rod, insert stirring rod till the bottom of motor axis
and tighten it with M2 screw.

Caution：
When inserting stirring rod, make sure the direction of force is
vertical to that of axis of rotating arm. Lateral force may damage the
axis or stirring rod.
Pushing the stirring rod completely

7 After stirring rod check, visually check whether stirring rod and its rotating arm
are vertical with each other.
If not vertical, return to step 5 and remount the stirring rod.
If vertical, continue to next.
8 Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm to the top of its rinsing bath.

Caution：
Please make sure to rotate stirring rod to its rinsing bath top after
mount it.
9 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample&reagent probe and
rinse it with deionized water. Observe the outflow of reagent&sample probe.
7.8.6 Check reaction cuvette

Warning：
Please be careful to avoid being scratched by sample&reagent probe.
Place each probe and pole into proper position for convenience.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective glasses
for the best
Please deal with removed reaction cuvette properly which is broken.
Caution：
Please use consumables recommended by DIRUI company, using other
consumables may cause system performance degradation.

1 Make sure the analysis part power is switched off.

70
2

Put on protective gloves to remove fixing screws.

3 Rotate reaction disk by hand and remove the reaction cuvette sequently. Take
out reaction cuvette while rotating it.

4 Rinse the new reaction cuvette dipped in with water; rinse inside and outside
of reaction cuvette and no scratch is allowed.
5 Rotate reaction disk by hands, and mount new reaction cuvette on reaction
disk and check the six sets reaction cuvette simultaneously.

6 Mount reaction disk with the opposite steps and make sure the fixing screw of
reaction disk is tight.
7 Switch on the power of analysis part

71
 Select and execute “cuvette blank test” after click “System maintenance”, and
observe execution result and reaction status.

Chapter 8 Analysis Method

CS-T240 adopts three analysis methods as follows:

End point analysis method

Two-points analysis method

Rate analysis method

8.1 Analysis principle

The analysis principle of auto-chemistry analyzer is based on the Beer-Lambert law that material
absorpts light selectively.

The main principle is: When monochromatic light with specific wavelength passes through the cuvette
with sample, the monochromatic light absorbency and sample liquid concentration vary in positivw
proportion as the distance of the light possing through the liquid.
I0
A = lg（1/T）= lg（ ）= ε b c
It
A －Absorbency of the light when passes through liquid

T －Transmitted intensity and incident intensity ratio: transmittance It/I0；

I0 － Incident intensity

It － Transmitted intensity

ε － Molar absorption coefficient of solution（ml×mmol－1×cm－1）

；

c － Mol concentration of the solution（mmol/ml）；

b － Solution layer thickness（cm）；

Solution layer thickness (b): Optical path, which is fixed by instrument. Molar absorption
coefficient (ε) is the correlation coefficient of the wavelength, solution and solution temperature.
Linear relationship is displayed between solution thickness and absorbency when in stable temperature
and single wavelength（ε value is given on the reagent bottle by factory）

72
 If the sample liquid adequate distribution, interaction between liquid and incidence
monochromatic light only happens during absorbing process. No fluorescence, disperse and
photochemical appear. No interaction between substances in the solution while absorbing process. The
absorbency possess conducts nature, and this condition conforms to the Beer-Lambert law

8.2 Types of analysis methods

Method Photometry point Cell Blank Absorbance Formula Remark

1-point
L– 0– 0– 0 B1 + B 2 + B 3 AL + AL −1
End point
analysis 1＜L≤49 3 2
method

2-points L– M– 0- 0 B1 + B 2 + B 3 ( AM + AM −1 ) − k ( AL + AL −1 )
analysis
1＜L＜M≤49 3 2
method

Time
2-points AM + AM −1 AL + AL −1 (minutes)
B1 + B 2 + B 3 −
Rate analysis L– M– 0– 0 3
2 2 between
method t metering
1＜L＜M≤49
points L,M

L– M – 0 - 0
B1 + B 2 + B 3
Rate method A 1＜L＜M≤49 △A（M-L）
3
L +2＜M

Fist half

L– 0 – 0 – 0 B1 + B 2 + B 3 AL + AL −1
3 2
1 ＜ M ＜ N≤L ＜ P ＜
1-point rate Q≤49
double items
Second half
analysis
method M – N – P – Q
B1 + B 2 + B 3
1 ＜ M ＜ N≤L ＜ P ＜ △（AQ- P）-k△（AN -M）
3
Q≤49

M+2＜N ，P+2＜Q

73
 Fist half
B1 + B 2 + B 3 AL + AL −1
L– 0 – 0 – 0
3 2
3-points 1＜L≤M＜N≤49
double items Second half
B1 + B 2 + B 3 ( AN + AN −1 ) − k ( AM + AM −1 )
M – N – 0 – 0
3 2
1＜L≤M＜N≤49

Fist half

L– M – 0 - 0 B1 + B 2 + B 3
△A（M-L）
3≤L＜M＜N＜P≤49 3
Rate B double
items L +2＜M
analysis
When it is different from the
method Assay Second half first half part wavelength of
(mode 1 ) the project △ A (P-N)
N – P – 0 – 0 B1 + B 2 + B 3 When it is the same with the Two conditions
3≤L＜M＜N＜P≤49 3 second part of wavelength of
the project: △A （P-N）–k
N+2＜P △A（M-L）

Fist half part of

the project

L– M – 0 – 0 B1 + B 2 + B 3
△A（M-L）
3≤L＜M＜N＜P＜Q＜ 3
R≤49
Rate B double
items L +2＜M
analysis
method Assay Second half part of
(mode 2 ) the project

N – P –Q – R B1 + B 2 + B 3
△A(R-Q)–k△A(P-N)
3≤L＜M＜N＜P＜Q＜ 3
R≤49

N+2＜P ，Q+2＜R

74
 L,m,n,p,q,r : Photometric points

Rn ：Volume of the reagent, n=1 to 4

B1、、
B 2 B 3 ：Through cell blank

(B1,B2,B3 )/3 ：Average value of three times passing through cell blanks

Ax ：Absorbance at photometric point x

△A(m-L) ：Change in absorbance per minute between photometric points L

and M

k ：Liquid volume correction factor

a
S + ∑ Rj
j =1
k= b
S + ∑ Ri
i =1

S ：Sample volume
Rj 、Ri: a is the volumn of reagents without correction

b is the volumn of reagents with correction

Note 1: After adding reagent 2, the 5th metering point does not immediately stir. But after the reaction
disk rotates one circle around plus 2 reaction cuvettes and then pause, it need to rotate another 22
reaction cuvettes, after which will pause and then stir.
Note 2: During photometry , the reaction liquid should be more than or equal to 150μL, and less than
or equal to 450μL.
Note 3: Be sure to enter "0(zero)" when there will be no photomitric point.

Endpoint analysis method

Endpoint analysis method is reaction takes a period of time to reach

equilibrium, due to reaction balance constants are big, all substrates (tested
substance) are transformed into product, and no increase (decrease) of
reaction solution absorbance will occur, and the degree of absorbance increase
(decrease) and the concentration of tested substance is directly proportional.
This method is called “endpoint analysis method” or balance analysis method
to be more accurately, which is the ideal analysis method mode. .

The endpoint analysis method is not sensitive to small changes of conditions

(such as enzyme amount, pH, temperature, etc.) as long as this change does
not affect the balance in a certain period of time.

figure 8-1 endpoint assay reaction curve

75
bs
A

Cell blank

Time

Example 1：TBIL－Total bilirubin reagent kit (Surfactant / diazonium salt method)

wavelength Main 550nm，sub 660nm Absorbance 0～2A；

range
test mode Endpoint analysis method Optical path 10mm
reagent 1：250uL sample 10uL
Single reagent A:B＝50:1 Mixing
reagent storage
temperature 37℃ incubation 10min
reaction 10min “0” 550nm，blank pipe
sensitivity 5mA equals to 1umol/L Linearity 300umol/L（18mg/dL）
range
calibrator 89.6umol/L，0.425A Unit 1umol/L＝0.0585mg/dL

76
 conversion
Reference Adult；5.1～19umol/L（0.3～1.1mg/dL）
value Newly born：20～200umol/L（1.2～12mg/dL）

Example 2、UA（uric acid）－Uric acid liquid reagent kit

wavelength Main 520nm （ 500,550 Absorbance 0～2A

optional） range
Test mode Endpoint analysis method Optical path 10mm
Reagent 1：200uL；2：50uL sample 4uL
Single 4 parts of reagent 1 part of Mixing storage 2～8℃ 5 days stablity
reagent reagent 2
temperature 37℃（30℃,25℃） incubation 5min（6min , 8min ）
reaction 5min adjust “0” 520nm，blank pipe
sensitivity 0.42mA equals to1umol/L Linearity range 1.5mmol/L（25mg/dL）
calibration 0.72mmol/L，0.302A Unit 1mmol/L＝16.8mg/dL
conversion
Reference child：0.12～0.33mmol/L（2.0～5.5mg/dL） ；
value Male：0.21～0.43mmol/L（3.5～7.2mg/dL） ；
female：0.15～0.36mmol/L（2.5～6.0mg/dL） ；
urine ：14.9～44.6mmol/L（250～750mg/dL） ；

8.4 Two Points analysis method

Two points analysis method (fixed time assay) is also named as first class
dynamics analysis method, which means during a certain period time of reaction,
reaction speed is in direct proportion to the simple power of substrate
concentration in specified time, namely v=k[S]. Due to the reduction of
substrate, the whole reaction speed is decreasing gradually, which shows the
increase (decrease) of absorbance. Because it takes a very long time to reach
balance, it can be monitored at any time theoretically, but due to the
complexity of serum ingredient, it must take a certain period of time to enter
into stable reaction phase.

Figure 8-2 2-point assay reaction curve

Reaction limit level

Absorbance

Absorbance

Cell blank

Time
Time

77
 Two points end point analysis two points rate analysis

8.5 Rate Analysis

Rate analysis, also known as zero-class dynamics analysis, refers to the

reaction rate is directly proportional to the zero power of substrate
concentration, which has nothing to do with the substrate concentration. Hence,
the reactants can generate a certain product at constant speed throughout the
reaction process, resulting in even decrease or increase of absorbance of
measured solution at a wavelength, and the decrease or increase speed (△ A
/ min) is directly proportional to the activity or concentration of the tested
substance (catalytic material). Dynamics assay is also called as the
continuous monitoring assay, mainly used for the measurement of enzyme
activity.

In fact, because substrate concentration is not high enough, with the reaction
proceeds, the reaction is no longer zero class when substrate is consumed to
a certain extent, Therefore, zero-class dynamics analysisn is targeted at a
certain period of time; Because reaction time to reach balance is very long,
it can be monitored at any time theoretically, but because of the complexity
of serum ingredient and much reaction, therefore, it takes a certain period
of time to enter in stable reaction phase. So all reagent manufactures have
strict requirements to thesse two time periods

Dynamics analysis is based on the changes between specified photometric points

to obtain the absorbance concentration or activity value.

Metering point in accordance with the input form, dynamics method can be
divided into single-band and dual-band dynamics analysis.

Figure 8-3 Rate assay reaction curve

Absorbance

Cell blank

Time

78
Example 3：ALT/GPT － Alanine aminotransferase （IFCC）

wavelength Main 340nm Absorbance range 0～2A

Test mode Rate assay Optical path 10mm
Reagent 1：240uL；2：60uL sample 15uL
Single reagent 4 reagent 1and 1reagent 2 Mixing storage 2～8℃ 5days stability
temperature 37℃ incubation 5min
reaction 60s delay，measure 60－120s “0” 340nm，blank pipe
sensitivity 0.30mA/min equals to 1.0U/L Linearity range 450U/L（7.5ukat/L）
calibration Unit conversion 1U/L＝16.67×10-3ukat/L
Reference value 37℃：Male：<40U/L（<0.67ukat/L） ；Female：<31U/L（<0.52ukat/L）

Calculating method：

�
A/min * TV *1000
ALT（U/L）＝
6.22 * SV * P

TV＝The total reaction volume (mL）

SV＝sample volume（mL）

P＝optical path of colorimetric cup（cm）

6.22＝NADH position mmol extinction coefficient at 340nm （334nm：6.18，365nm：

3.40）

79
8.6 Principle of electrolyte measurement

8.6.1 Principle

Internal standard solution is firstly added into the instrument reaction

cuvettes, and assimilates it and discharges it into the Na, K, and Cl electrode
solution line through the SIP injection pump to measure its electrode potential
which is relative to reference electrode potential. Then needle draws the
samples and then sample is diluted then added to the reaction cuvette. To test
liquid electric potential mixed with electric potential, the calculation of
the concentration comes out.

8.6.2 Principle of generating electrode potential

Electrode potential is obtained by Nernst's formula.

RT
E =E 0 + 2.303 × × l og(ai )
nF ………………………………………………………（1）

ai= f × Ci ……………………………………………………………………………（2）

E 0 : The standard potential of the measured system

R: gas constant (8.314510 J × mol-1 × K-1)

80
 T: absolute temperature (t ℃ +273.15) (K)

F: Faraday constant (9.6485309 × 104 C × mol-1)

ai : Ion (i) activity

f: activity coefficient

Ci: concentration

n: a given ion (i) the charge number (Cation is positive, anion is

negative)

8.6.3 Test method

Working curve preparation, the internal standard solution concentration

measurement, concentration calculation, and result modification are explained
as follow.
8.6.3.1 Working curve preparation

Measure low concentration slope of liquid (S1) and high concentration slope
liquid (S2), and determine slope value (sensitivity) of K, Na, Cl the
electrode.

E ( H ) − E ( L)
SL =
C(H )
log
C ( L) …………………………………………………… (3)

SL: slope value (slope)

E (H): the potential of high concentration slope solution

E (L): the potential of low concentration slope solution

C (H): high concentration of the concentration slope solution (input value)

C (L): low concentration of the concentration slope solution (input value)

8.6.3.2 The measurement of internal liquid concentration

E ( IS ) − E ( L )
C (=
IS ) C ( L) ×10 SL
…………………………………………… (4)

81
 C (IS): the concentration of internal standard solution

E (IS): the potential of internal standard solution

8.6.3.3 concentration calculation

The calculation of routine sample, STAT sample, and concentration of quality

control liquid is based on the concentration of internal standard solution.
Internal standard solution is different with the different sample.

E ( S ) − E ( IS )
=
C ( S ) C ( IS ) ×10 SL
………………………………………… (5)

C(S) : Sample concentration

E(S): Sample potential

8.6.3.4 result modification

Test calibrator (calibrator S3)of serum category after calibration to

calculate its concentration, and the difference between the tested
concentration and input value is used as compensation value to increase or
decrease sample quantitative value.

=
C (VALUE ) C (C ) − C ( X ) ………………………………………… (6)

C (VALUE): compensation value (compensation value)

C (C): Concentration input value of the serum calibrator

C (X): Concentration tested value of the serum calibrator

C ' ( S ) IF {C ( S ) + C (VALUE )}
=
.. ... ... ... ... ... ... ... ... ... ... ..
. ... ... (7)

C '(S): modified sample concentration

IF: Instrument constant (usually 1.0)

8.6.3.5 Standard specification of electrolyte

Item specification

Sample volume 15ul

82
 Diluent volume 600ul
Processing
100samples/h（only measuring electrolyte）
ability
Na + 20 ~ 200mmol / L (when only serum )
10 ~ 400mmol / L (when measuring urine)
K + 1.0 ~ 15.0mmol / L (when only serum)
Measuring
1 ~ 200mmol / L (when measuring urine)
range
Cl-20 ~ 200mmol / L (when only serum)
10 ~ 400mmol / L (when measuring urine)

Internal standard solution 600ul/sample

Reagent
(only for continuous determination of electrolyte )
consumption
Diluent 600ul /sample
volume
Reference Electrode Solution 130 ul / sample

Note:

Internal standard solution will be added one time in order to activate the
electrode if there is no electrolyte analysis for more than 10 minutes.

83
 Chapter 9 Troubleshooting List

9.1 Stirring mechanism malfunction analysis

Alarm Detailed
Description Solution
Code description

 In the CS-T240 software, enter "system maintenance"

interface after getting on-line, implement "mechanical
movement check", and observe the stirring rod
running status

Malfunction 1：stirring mechanism doesn’t move

Solution:

1, Check stirring mechanism up-down movements,

mechanical repair is required if resistance is big.

2, check whether both ends of connector of the motor are

connected well.

3, check whether the conductivity of motor lead wire is

stirring good.
mechanism
stirring 4, check the motor drive module of circuit board is
fails to reach
1-1 mechanism working normally
the top of
abnormal
rinsing bath
side. Malfunction 2：stirring mechanism can reach the top, but
can not check the zero position.

solution：Manually make stirring mechanism repeatedly rise

to peak, DC files transferred to the multimeter, plug the
negative point of the first 6-core P02, P02 positive pole
core plugs 5

1、Multimeter readings between the positive changes in 5V

and 0

(1) Check whether the conductivity from adapter to reaction

disk circuit board is good and both ends of connector are
connected well.

(2) Check input part circuit of reaction disk circuit board

light sensor signal.

84
 2、No change on multimeter reading

(1) Check conductors

Check whether the P02 plug 4,5,6 core well-connected, the

connection is connected at both ends of good

(2) Check the coupler signal

Check voltage between P02 plug 4,6 feet, if the voltage is

not 5v, check the connection plug is connected good. If the
voltage is 5v, check the J02 socket 5 feet of potential,
rising to peak when the potential was high or low potential.
If the potential is not normal, check the coupler

Malfunction 3：Stirring mechanism cannot reach the zero

position.

solution：Mechanical repair

 In the CS-T240 software, enter into the "system

maintenance" interface after on-line, implement
"mechanical movement check", and observe the
stirring rod running status
Malfunction 1：stirring mechanism stops
solution：

1, Check stirring mechanism up-down movements,

mechanical repair is required if resistance is big.
stirring 2, check whether both ends of connector of the motor are
Stirring mechanism connected well.
1-3 mechanism can leave the
abnormal top when it 3, check whether the conductivity of motor lead wire is
descents good.

4, check the motor drive module of circuit board is

working normally

Malfunction 2：stirring mechanism can reach the top but not

leave

Solution ：the same to 1－1 Malfunction 2

 In the CS-T240 software, enter into the "system

stirring
maintenance" interface after on-line, implement
mechanism
"mechanical movement check", and observe the
Stirring fails to reach
stirring rod running status
1-4 mechanism the rinsing
abnormal bath when it Malfunction 1：：stirring mechanism doesn’t move
moves to
Solution：
rinsing bath.
1, Check stirring mechanism up-down movements,

85
mechanical repair is required if resistance is big.

2, check whether both ends of connector of the motor are

connected well.

3, check whether the conductivity of motor lead wire is

good.

4, check the motor drive module of circuit board is

working normally

5、Check whether the it is at top position, check whether

up-down zero light sensor works normally at top position.

Malfunction 2： stirring mechanism can sway, but can not

reach rinsing bath.

Solution：

1、check whether the installation of left and right limit light

sensors is correct.

2、When the instrument is turned on, manualy sway the

stirring mechanism to observe the right limit coupler,
transferr the multimeter to DC, negative points P02 core
plug No.9, then plug positive P02 to the first pole core No.8.

(1）Multimeter readings changes between 5V and 0, the light

sensor, lead and adaptor are normal.

1) Check conductors

Check whether the P02 plug 7,8,9 core well-connected, the

connection is connected at both ends of good

2) Check the reaction plate coupler circuit board part of the

circuit signal input

(2）No change multimeter reading

1) Check conductors

Check whether the P02 plug 7,8,9 core well-connected,

the connection is connected at both ends of good

2) Check the coupler signal

Check voltage between P02 plug 7,9 feet, if the voltage is

not 5v, check the connection plug is connected good. If
the voltage is 5v, check the J02 socket 8-foot potential, up
to the peak when the potential was high or low potential.
If the potential is not normal, check the coupler.

86
  In CS-T240 software, enter into the "system
maintenance" interface after on-line, implement
"mechanical movement check", and observe the
stirring rod running status
Malfunction 1：stirring mechanism doesn’t move

Solution：

1, Check stirring mechanism up-down movements,

mechanical repair is required if resistance is big.

2, check whether both ends of connector of the motor are

connected well.

3, check whether the conductivity of motor lead wire is

good.

4, check the motor drive module of circuit board is

working normally

5、Check whether the it is at top position, check whether

up-down zero light sensor works normally at top position.

Malfunction 2： Stirring mechanism can realize its sway

stirring movement, but can not reach the reaction cuvette.
mechanism
Stirring fails to reach Solution：
1-5 mechanism the reaction
abnormal cup when it 1、check whether the installation of left and right limit light
moves to sensors is correct.
reaction cup.
2、When the instrument is turned on, manually swing the
stirring mechanism to observe the left limit coupler, transfer
the multimeter to DC, negative points, 12 of the plug core
P02, P02 plug is the 11th pole core. .

(1) multimeter reading in is between 5V and 0 change,

indicating coupler, lead normal
1) Check conductors
Check whether the P02 plug 10,11,12 core well-connected, the
connection is connected at both ends of good
2) Check the reaction plate coupler circuit board part of the
circuit signal input
(2) multimeter reading of no change
1) Check conductors
Check whether the P02 plug 10,11,12 core well-connected, the
connection is connected at both ends of good
2) Check the coupler signal
Check voltage between P02 pin plugs 10,12, if the voltage is
not 5v, check the connection plug is connected good. If the
voltage is 5v, check the J02 socket 11 feet of potential, rising to
peak when the potential was high or low potential. If the
potential is not normal, check the coupler.

87
 When
resetting,
Stirring Stirring
1-6 mechanism mechanism The solution is the same as 1-4
abnormal failed to
reach rinsing
bath.

 Enter into system maintenance window and execute

“Mechanical movement check”. Observe the running
When status of stirring rod.
resetting, Malfunction 1：stirring mechanism doesn’t move
Stirring Stirring
1-7 mechanism mechanism The solution is the same as 1-4
abnormal failed to
leave rinsing Malfunction 2： Stirring mechanism can realize its sway
bath. movement to rinsing bath, but can not leave it.

The solution is the same as 1-4

Stirring
mechanism
Stirring
failed to  If stirring mechanism failed to reach the top when
1-8 mechanism
reach the top rotating，the solution is the same as 1-1
abnormal
when
rotating.

9.2 Rinsing mechanism of reaction cup

Alarm Detailed
Description Solution
Code description

 Enter into system maintenance window and execute

“Mechanical movement check”. Observe the
running status of stirring rod.
Malfunction 1：Rinsing mechanism doesn’t move

Solution:
Rinsing
Rinsing 1, Check rinsing mechanism sway movements,
mechanism of
mechanism of mechanical repair is required if resistance is big.
3-1 reaction cup
reaction cup
fails to reach the
abnormal 2, check whether both ends of connector of the motor are
top
connected well.

3, check whether the conductivity of motor lead wire is

good.

4, check the motor drive module of circuit board is

working normally

88
 Malfunction 2：Rinsing mechanism can reach the top,
but can not check the zero position.

solution：Manually make rinsing mechanism repeatedly

rise to peak, transferred the multimeter To DC, plug the
negative point of the first three core P02, P02 plugs are 2
pole core.

1、Multimeter readings between the positive changes in

5V and 0, indicating coupler, lead normal

1) Check conductorsCheck 1,2,3 P02 core plug is

connected well and good connections at both ends is
connected

2) Check the reaction plate coupler circuit board part of

the circuit signal input

2、 No change on multimeter reading

1) Check conductors
Check 1,2,3 P02 core plug is connected well and good
connections at both ends is connected

2) Check the coupler signal

Check voltage between P02 plug 1,3 feet, if the voltage
is not 5v, check the connection plug is connected good. If
the voltage is 5v, check the J02 socket 2 feet of potential,
rising to peak when the potential was high, or low
potential. If the potential is not normal, check the coupler

Malfunction 3：Rinsing mechanism can not reach the zero

position.

solution：Mechanical repair

 Enter into system maintenance window and execute

“Mechanical movement check”. Observe the
running status of stirring rod.
Malfunction 1：stirring mechanism doesn’t move
Rinsing Solution:
mechanism of
Rinsing reaction cup 1, Check stirring mechanism up-down movements,
3-2
mechanism fails to leave the mechanical repair is required if resistance is big.
abnormal top when
descending. 2, check whether both ends of connector of the motor are
connected well.

3, check whether the conductivity of motor lead wire is

good.

4, check the motor drive module of circuit board is

working normally

89
Malfunction 2：Rinsing mechanism of reaction cup
cannot reach the top

solution：the same to that of malfunction 2 in 3-1

90
9.3 Reaction disk malfunction analysis

Alarm Detailed
Description Solution
Code description

Malfunction：The quantity counted by reaction cup light

sensor does not conform to real quantity of cup rotated.

solution：Check whether the signal status of counting light

Reaction disk fails sensor is normal.
Reaction
to rotate to the
4-1 disk
designated
（1）check whether the conductivity from counting light
abnormal sensor 1 to lead wire is good.
position.
（2）check whether the counting light sensor 1 is ok.

（3）Check input part circuit of reaction disk circuit board

light sensor signal.

Reaction disk fails Malfunction：Reaction disk fails to stop at the position

Reaction
to stop at the matching with light sensor.
4-2 disk
designated
abnormal
position solution：the same as 4－1

Malfunction：Reaction disk fails to stop at the zero

position after resetting or the light sensor of zero position
did not check resetting point.

solution：Check whether the signal status of counting light

Reaction disk fails sensor is normal.
Reaction
to stop at the zero
4-3 disk
position when
（1）check whether the conductivity from zero light sensor
abnormal to lead wire is good and the both ends of connector are
resetting.
connected well.

（2）check whether the zero light sensor 1 is ok

（3）Check input part circuit of reaction disk circuit board

light sensor signal.

cleaning Liquid level of

4-6 liquid level alkaline cleaning Add cleaning liquid into cleaning liquid kit.
low liquid kit low.

91
9.4 Sample probe mechanism malfunction analysis

Alarm Detailed
Description Solution
Code description

Enter system maintenance window, execute "mechanical

inspection." Observe running probe arm

Malfunction 1: no liquid in sample cup

Sample Solution:
probe (1) confirm whether there are samples left int the sample
5-3 Sample abnormal. malfuntions cup.
while Malfunction 2: there are liquid in the sample cup
descending Solution:
(1) confirm the correct location of the sample cup.
(2) check and confirm whether the detection board of the
liquid is working ok

Malfunction 1: no putting of the sample cup in the sample

position
Solution:
Sample Alarm
probe Malfunction 2: putting of the sample cup in the sample
5-13 Sample abnormal. cannot find position
reaction Solution:
cuvette (1) Check whether there are sampel cup in the current
sample tray.
(2) check and confirm whether the detection board of the
liquid is working ok

9.8 Reagent probe mechanism malfunction analysis

Alarm Detailed
Description Solution
Code description

Enter system maintenance window of the operation

system, execute "reset". Observe probe running status

Malfunction 1: The arm of the probe couldn’t move

Sample&reage Solution:
nt probe 1 Manually move the probe mechanism, need mechanical
Reagent probe repair if resistance is big
8-1 couldn’t reach
abnormal.
the top while
rising 2, check whether the motor connector body is connected at
both ends in a good well function way
3, check whether the connection of electrical conductor is
good
4, check the sample&reagent motor drive circuit board
Malfunction 2:The probe arm to the coupler's position

92
 can run, but the photo sensor cannot detect any signal
Solution: Manually make the probe body go up to the
peak repeatedly, transfer the multimeter to DC, negative
points the first 6-core plugs P042, P042 positive pole core
plugs 5.
1, multimeter readings between the positive changes from
5V to 0, indicating photo sensor and lead are normal
(1) Check conductors
Check whether the sample probe’s photo sensor J202 to
the reagent disc circuit board (P042) is in good
connection, and whether the both ends of the connectors
are good.
(2) Check the input of the sample reagent disc circuit
board’s photo sensor.
2, No change on multimeter reading
(1) Check conductors
Check P042 plugs 4,5,6 core is connected well,
connection of both ends is good.
(2) Check the photo sensor
Check voltage between P042 pin plugs 4,6, if the voltage
is not 5v, check the connection plug is connected well. If
the voltage is 5v, check the J042 socket 11 feet’s potential.
Rising to the peak when the potential is high, if not, the
potential is low. If the potential is not normal, check the
photo sensor.

Malfunction: When Sample&reagent probe’s running, it’s

touching the side of the reagent cup or the botom of the
cup
Solution:
Observe the Sample&reagent probe’s running status
Probe senses (1) If the Sample&reagent probe is touching the side of
Reagent probe the liquid the cup while it’s running, need to ajust the position of the
8-2 Sample&reagent probe’s and the position of the cup
abnormal. touches the
side (2)If the probe directly touches the botom of the cup while
it’s running, check whether there is reagent in the reagent
cup. If not, need to add reagent and then check. If yes,
need to check the circuit board of the liquid surface, check
whether the sensitivity of the Sample&reagent probe is
working normally.

Enter system maintenance window of the operation

system, execute "reset". Observe probe running status
Sample&reage Malfunction 1: The arm of the probe couldn’t move
nt probe Solution: 1 Manually move the probe mechanism, need
Reagent probe
8-3 couldn’t leave mechanical repair if resistance is big
abnormal.
the top while
descending 2, check whether the motor connector body is connected at
both ends in a good well function way
3, check whether the connection of electrical conductor is
good

93
 4, check the sample&reagent motor drive circuit board

Malfunction 2: probe arm to the coupler's position can

run, but the photo sensor cannot detect any signal
Solution: 1, multimeter readings between the positive
changes from 5V to 0, indicating photo sensor and lead
are normal
(1) Check conductors
Check whether the Sample&reagent probe’s photo sensor
J202 to the reagent disc circuit board (P042) is in good
connection, and whether the both ends of the connectors
are good.
(2) Check the input of the sample&reagent disc circuit
board’s photo sensor.
2, No change on multimeter reading
(1) Check conductors
Check P042 plugs 4,5,6 core is connected well,
connection of both ends is good.
(2) Check the photo sensor
Check voltage between P042 pin plugs 4,6, if the voltage
is not 5v, check the connection plug is connected well. If
the voltage is 5v, check the J042 socket 11 feet’s potential.
Rising to the peak when the potential is high, if not, the
potential is low. If the potential is not normal, check the
photo sensor.

Malfunction 1: Sample&reagent probe is in the effective

status of touching and liquid detecting all the time

Solution: If the arm of the probe is not moving,

repeatedly and manually pull up the probe (imitate the
touching), repeatedly touch the tip of the probe
gently(imitate the liquid detection), transfer the multimeter
to DC, negative point of P042 plug No.10 core, positive
point P042 plug No.9 core
Sample&reage
nt probe is in Initial State: Multimeter reads “0”
the effective
Reagent probe status of Touching: Multimeter reads “1”
8-4
abnormal. touching and 1. Multimeter reads between 5V and 0, indicates that the
liquid photo sensor and the lead are normal
detecting all
the time (1) Check the lead

Check the connection of probe’s photo sensor J202 to the

sample&reagent circuit board (P042)

check whether the connection of probe photo sensor J202

with sample&reagent dick circuit board wire（Ｐ042）is
good or not, check the connection of the both ends.

(2) check reagent dick circuit board

94
 2. No chang on multimeter readings

(1) Check the liquid level detection of flexible cable is

connected well, check the conductivity

(2) Check the liquid level detection signal line (P042) is in

good connection or not, check conductivity
(3) Check whether the liquid level detector is well
connected or not
(4) measurement of P042 in the 8 (surface), 9 (touch) pin
voltage is normal or not.

When liquid surface is detected, P042-8 surface is high

level
When liquid surface is not detected, P042-8 surface is low
level
When touches, the photo sensor blocks, P042-9touch is
high level,
No touch, the photo sensor is without shelter, P042-9touch
is low level.
(5) Check the P042's 8 (surface), 9 (touch) feet whether 7
(+5 v) is short-circuit, so that potential is pulled up.
(6) Check the liquid level detection board is working
regularly, when the status is not , check the circuit board.
Unplug the surface detection panel J6, J7 on the flexible
cable, measuring J6, J7-level state of the foot, right to left
(J7: 1 empty, 2touch, 3surface) (J6: 1 ground, 2 air, 3 +5
v, 4 blank)
When liquid level is detected, J7-3surface is high, and the
on-board indicator lights;
When liquid is not detected, J7-3surface is low, on-board
light goes out;
When there’s touch, the photo sensor blocks, and
J7-2touch is high;
When there’s no touch, the photo sensor is not blocked,
J7-2touch is low.

Enter CS-T240 operation system, system maintenance

When window, execute "mechanical movement". Observe
Sample&reage sample&reagent probe running status.
nt probe
changes its Malfunction 1: Sample&reagent probe couldn’t sway.
direction to
cup side, and Solution:
Reagent probe couldn’f find
8-5 1. Manually sway the mechanism, need mechanical repair
abnormal reaction disk’s
position. if resistance is big
(Sample&reag
2. Check both ends of the motor connector are connected
ent probe
well
swings can not
3, Check whether the conductivity of the conductor’s lead
be set back as
are good
zero)
4, Check the sample&reagent disc motor drive circuit
board

95
 5, Check if the up down and back to zero of photo sensor
are working normally

Malfunction 2: When the arm sways to reaction disk

position, photo sensor couldn’t detect signal

Solution: Manually make the probe mechanism rise to

peak repeatedly, transfer the multimeter to DC, negative
point P042 core plug No.3, positive point P042 plugs to
core plug No. 2.

1, multimeter readings between the positive changes in 5V

and 0, indicates photo sensor and lead are normal

(1) Check conductors

Check conductivity of probe photo sensor J202 to the

reagent disc circuit board (P042) is connected good or not,
check both ends of the connector are connected well or
not.

(2) Check sample&reagent disc circuit board’s photo

sensor’s input electric circuit

2, No change on multimeter reading

(1) Check lead

Check plug P042 core plug 1,2,3 is connected well or not

and whether both ends of the connection wire is good or
not

(2) Check photo sensor signal

Check voltage between P042 pin plugs 1,3, if the voltage

is not 5v, check the connection plug is connected well or
not. If the voltage is 5v, check the J042 socket 2 feet of
potentials, when it is up to peak, when the potentials is
high, if not, potential’s low. If the potential is not normal,
check photo sensor.

Malfunction 3: Couldn’t sway to reaction disk position

Solution: mechanical repair

Enter CS-T240 operation system, system maintenance

When the window, execute "mechanical movement". Observe probe
Sample&reage running status.
nt probe
Reagent probe switches to Malfunction 1: Sample&reagent probe couldn’t sway
8-6
abnormal another
location, it Solution：same as to 8-5
couldn’t leave
reaction disk Malfunction 2：The arm can leave reaction disk position,
however, the photo sensor couldn’t detect any signal

96
 Solution：same as to 8-5

When
Sample&reage
Reagent probe nt probe
8-7 Probe couldn’t rise to the peak, solution same as to 8-1.
abnormal rotates, it
deciates from
the peak

Malfunction: When there is liquid in the reagent bottle,

Sample&reagent probe cannot detect it

Solution:

it couldn’t 1, Check the liquid level detection reagent with the sample
Reagent probe detect liquid plate to connect the circuit board is the connection of a
8－8
abnormal surface good guide, the connector ends are good
existence
2, check the liquid level detection circuit board, test the
sensitivity of the sample reagent is normal needle

3, check the sample reagent circuit board part of the

electrical signal input

Malfunction: Cannot reach the height of probe arm

falling liquid reagent
Solution:
1, Manually move the probe mechanism, if the resistance
is big, mechanical repair is needed
Cannot reach 2, Check the liquid level detection plate flexible cable is
Reagent probe
8-9 the liquid drop connected well or not
abnormal
height 3, Check both ends of the electrical connector body is
connected well
4, Check whether the connection of electrical conductor
lead is good
5, Check whether there is malfuction of surface detection
6, Check the sample kit circuit board

9.10 Sample&reagent disk malfunction analysis

Alarm Detailed
Description Solution
Code description

Malfunction 1: Sample&reagent disk doesn’t move

Sample&
Sample&reagen Solution:
10-2 reagent disk is
t disk abnormal 1, Manually rotate the sample&reagent disk, if the
not stop at the
resistance is big, mechanical repair is needed
specified
2, Check both ends of the motor connector is connected

97
 location well
3, Check the conductivity of the electrical conductor lead
is good
4, Check sample&reagent circuit board’s motor drive
circuit board kit part
Malfunction 2: When sample&reagent disc rotates to the
specified location, the sample&reagent disk does not stop
at the specified position, or when turns to specified
location, the photo sensor couldn’t detect it, or the signal
of the photo sensor is not correct

Solution:

Manually rotate the sample&reagent disk, transfer the

multimeter to DC, negative points P041, core plug No.6,
positive points P041, core plugs No.5

1, Multimeter readings between the positive changes in

5V and 0, indicates photo sensor and lead are normal

(1) Check lead

Check the conductivity of probe photo sensor J047 to

sample&reagent disk circuit board lead (P041) is good or
not, check both ends of connector is connected good or
not

(2) Check photo sensor’s electric circuit input of sample&

reagent disk’s circuit board

2, No change on multimeter reading

(1) Check wire

Check P041 plugs 4,5,6 core is connected well, a good

connection is connected at both ends

(2) Check the photo sensor signal

Check voltage between P041 pin plugs 4,6, if voltage is

not 5v, check the whether the connection plug is
connected well or not. If the voltage is 5v, check the J041
socket 5 feet of potential, raise up to the peak when the
potential is high, if not, the potential is low. If the
potential is not normal, check the photo sensor.

Enter into system maintenance window, execute

“mechanical movement check”, observe disk retoating
Cannot find status
the sample&
Sample&reagen Malfunction 1: The sample&reagent disk doesn’t move
10-3 reagent disk
t disk abnormal Solution:
position of
back to zero 1. Manually rotate the sample&reagent disk, need
mechanical repair if the resistance is big

2. Check both ends of the motor connector are connected

98
 well of this mechanism
3, Check whether the conductivity of the conductor’s lead
are good
4, Check the circuit board of the sample reagent disk

Malfunction 2: Sample reagent disk rotates, back

couldn’t find the position of back to zero

Solution: Manually rotate the sample&reagent disk,

transfer the multimeter to DC, negative points P041 core
plug No.3, positive points P041 core plug No.2

1. If multimeter reading is between positive 0 to 5V,

indicates that the photo sensor and the lead are normal

(1)Check the lead

Check the conductivity of probe photo sensor J047 to the

sample reagent disk’s circuit board lead (P041) is good or
not, check both ends of the connector are connected well
or not

(2)Check photo sensor’s electric circuit input of sample&

reagent disk

Check voltage between P041 pin plugs 1,3, if voltage is

not 5v, check whether the connection plug is connected
well or not. If the voltage is 5v, check the J041 socket 2
feet of potential, raise up to the peak when the potential is
high, if not, the potential is low. If the potential is not
normal, check photo sensor.

Malfunction 1: Sample&reagent disk couldn’t find

barcode reader
Solution:

Sample&reagen Couldn’t find 1, Check whether the barcode reader is well connected to
t disk barcode sample the instrument
10-4
reader reagent
abnormal barcode reader 2, Check whether the barcode reader is broken
Please reset the barcode reader, if it still doesn’t work,
check barcode reader. If it starts to work normally,
indicates that there is no problem on the barcode reader. It
might be the problem of digital wire or circuit board.

Malfunction 1: Current turntable position error

Motor running Solution:
Sampel&reagen
steps are
t disk turntable 1,Check whether there are friction between sample reagent
10-7 incompatible
motor steps disk shelf and sample reagent refrigeration warehouse
with the given
abnormal
steps number
2,Check whether sample&reagent disk fram has obstacles
that affect rotation

99
9.12 Injection pump malfunction analysis

Alarm Detailed
Description Solution
Code description

Malfunction 1: Syringe pump doesn’t move or it couldn’t

rise up to the top
Solution:
1. When the instrument is working, the syringe pump
Syringe pump doesn’t work
Syringe pump
14-1 doesn’t rise up
abnormal (1) Check both ends of the mechanism’s motor are
to the top
connected well

(2) Check thec conductivity of the motor lead is well

(3) Check cicrcuit board’s motor drive lead

Malfunction 1: Pump couldn’t move or it cannot leave

the top

Solution:

1. Observe the moving status of the syring pump while it’s

working
(1) Check both ends of this mechanism motor are
connected well

(2) Check the conductivity of the motor lead is good or

not

(3) Check circuit board’s driving motor

Syringe pump
Syringe pump 2. While the instrument is working, if the motor is
14-2 couldn’t leave
abnormal working ok, and it can leave the photo sensor position
the top
(1) Check whether the photo sensor is in good condiction

(2) Check the conductivity of the lead connects the photo

sensor and the power adapter plate is good or not, check
both ends of the connector are connected well or not.
(3) Check the signal of the photo sensor
Check voltage between P043 pin plugs 1,5, if the voltage
is not 5v, check the plug P043 is in good connection; if the
voltage is 5v, check the potential of P043 4-pin socket.
While the pump is back to zero (vomiting), then the
potential is high, if not, the potential is low. If the potential
is normal, check the adapter plate; if it’s abnormal, check
photo sensor.

100
9.14 Incubation bath malfunction analysis

Alarm Detailed
Description Solution
Code description

(1) Make sure the cooling fan of the instrument is rotating

normally
The
Incubation bath temperature of (2) Check the temperature sensor is working normally
20-1 temperature incubation
(3) Check the connector part of the temperature sensor on
abnormal bath is above
the main control board
45℃
(4) Replace the main control board or the temperature
sensor

The
temperature of
the incubation (1) Make sure the room temperature is in the scope of
bath is out of 15-32
the scope of
Incubation bath (2) Make sure the cooling fan of the instrument is rotating
20-2 temperature 37℃ ±5℃
narmally
abnormal
(Only check
(3) Make sure the water is cycling in the incubation bath
when the
instrument (4) Replace main control board or the temperature sensor
under
operation)

9.15 ISE Failure Analysis

ISE QC is
over
ISE is over measuring （1）Please check whether the Control
0-31 Note measuring lowe lower limit， volume, reagent volume is adequate, and
limit the current whether the position is correct
QC has no
result.

ISE QC is
ISE is over over （1）Please check whether the Control
0-32 Note measuring measuring volume, reagent volume is adequate, and
upper limit upper limit， whether the position is correct
the current
QC has no

101
 result.

ISE
Calibration
is over （1）Please check whether the Calibrator
ISE is over measuring volume, reagent volume is adequate, and
0-33 Note measuring lower limit， whether the position is correct
lower limit the current
Calibration
has no
result.

ISE
Calibration
is over
ISE is over measuring （1）Please check whether the Calibrator
0-34 Note measuring upper limit， volume, reagent volume is adequate, and
upper limit the current whether the position is correct
Calibration
has no
result.

ISE sample
is over
ISE is over measuring （1）Please check whether the sample
0-35 Note measuring upper or volume, reagent volume is adequate, and
range lower limit， whether the position is correct
no test
result.

Electrolyte
system
stops
working
due to the （1）Enter into system maintenance, and
issued execute "Resetting”, and then execute
Electrolyte
19-1 Note function stops
alarm. “Mechanical movement check”
Note: the
stop status
of adding
sample
means to
restart.

Remaining （1）add ISE reference solution

volume of
ISE reference reference （2）Enter into system maintenance
37-1 Note liquid is solution is window, and execute "ISE reference
inadequate inadequate solution reagent pipeline rinsing”
(less then
the user （3）Execute ISE calibration
designed

102
 volume)

remaining
volume of
internal （1）add ISE internal standard solution
ISE internal standard
（2）Enter into system maintenance, and
standard solution is
38-1 Note liquid is inadequate execute "ISE internal standard solution
inadequate (less then reagent pipeline rinsing”
the user
（3）Execute ISE calibration
designed
volume)

remaining
volume of （1）add ISE diluent
diluent is
ISE diluent is （2）Enter into system maintenance, and
inadequate
39-1 Note (less then
execute "ISE diluent reagent pipeline
inadequate rinsing”
the user
designed
（3）Execute ISE calibration
volume)

In the
5-measurin
g point

potential of
internal
standard (1) Enter into system maintenance
solution, the window, and execute "ISE check."
average
value
ISE LEVEL
60-1 Note (2) please refer to " electrolyte device
error (EAV)of maintenance " in the "user manual" for
three detail.
potentials is
over the
following
range

（internal
standard
solution）K：
-90.0mv≤E
AV≤-10mv

In the (1) Enter into system maintenance, and

ISE LEVEL
60-2 Note 5-measurin execute "ISE check."
error g point

103
 potential of (2) please refer to " electrolyte device
internal maintenance " in the "user manual" for
standard detail.
solution, the
average
value

(EAV)of
three
potentials is
over the
following
range

（internal
standard
solution）K：
-90.0mv≤E
AV≤-10mv

In the
5-measurin
g point

potential of
internal
standard
solution, the
average
value (1) Enter into system maintenance, and
execute "ISE check."
(EAV)of
ISE LEVEL three
60-3 Note potentials is (2) please refer to " electrolyte device
error maintenance " in the "user manual" for
over the
following detail.
range

（internal
standard
solution）
Cl：
80.0mv≤EA
V≤160mv

In the
(1) Enter into system maintenance, and
5-measurin
execute "ISE check."
ISE Noise g point
61-1 Note
error
potential of
(2) please refer to " electrolyte device
internal
maintenance " in the "user manual" for
standard

104
 solution, the detail.
difference
（FI） of
the
maximum
and
minimum is
within
following
range

Na：
0.7mv≤|FIV
（2）-FIV
（4）|

In the
5-measurin
g point

potential of
internal
standard
solution, the
difference
（FIV） of (1) Enter into system maintenance, and
the execute "ISE check."
maximum
ISE Noise
61-2 Note and
error minimum is (2) please refer to " electrolyte device
within maintenance " in the "user manual" for
following detail.
range
(internal
standard,
sample)

K：
1.0mv≤|FIV
（2）-FIV
（4）

In the
5-measurin (1) Enter into system maintenance, and
g point execute "ISE check."

ISE Noise potential of

61-3 Note internal (2) please refer to " electrolyte device
error maintenance " in the "user manual" for
standard
solution, the detail.
difference
（FIV） of
the

105
 maximum
and
minimum is
within
following
range
(internal
standard,
sample)

Cl：
0.8mv≤|FIV
（2）-FIV
（4）|

The slope
value of
calibration
result is
within
following
range or the
impact of
electrode is
low (1) Enter into system maintenance, and
(cross-conta execute "ISE check."
ISE mination
62-1 Note
Prepare rate (A) is (2) please refer to " electrolyte device
as maintenance " in the "user manual" for
abnormal following detail.
Na：（1）
32.0mv≤SL
OPE≤37mv
or
68.1mv≤SL
OPE

The slope
value of
calibration (1) Enter into system maintenance, and
result is execute "ISE check."
ISE within
following
62-2 Note Prepare range or the
(2) please refer to " electrolyte device
maintenance " in the "user manual" for
abnormal impact of
detail.
electrode is
low
(cross-conta
mination
rate (A) is

106
 as
following
K：（1）
32.0mv≤SL
OPE≤37mv
or68.1mv≤S
LOPE

The slope
value of
calibration
result is
within
following
range or the
impact of
electrode is (1) Enter into system maintenance, and
execute "ISE check."
ISE low
(cross-conta
62-3 Note Prepare mination (2) please refer to " electrolyte device
abnormal rate (A) is maintenance " in the "user manual" for
as detail.
following

Cl：（1）
-30mv≤SL
OPE≤-25m
v
or-68.1mv≥
SLOPE

（1）The
slope value
of
calibration
result is
within
following (1) Enter into system maintenance, and
range or the execute "ISE check."
ISE slope impact of
value electrode is
(2) please refer to " electrolyte device
63-1 Note low
（SLOPE） (cross-conta
maintenance " in the "user manual" for
abnormal detail.
mination
rate (A) is
as (3) Please re-execute ISE calibration.
following:

Na：（1）
SLOPE＜
32.0mv

（2）current

107
 ISE
calibration
result is not
updated

（1）The
slope value
of
calibration
result is
within
following
range or the
impact of (1) Enter into system maintenance, and
electrode is execute "ISE check."
ISE slope low
value (cross-conta
(2) please refer to " electrolyte device
63-2 Note mination
（SLOPE） rate (A) is
maintenance " in the "user manual" for
abnormal detail.
as
following:K
：（1） (3) Please re-execute ISE calibration.
SLOPE＜
32mv

（2）current
ISE
calibration
result is not
updated

（1）The
slope value
of
calibration
result is
within
following (1) Enter into system maintenance, and
range or the execute "ISE check."
impact of
electrode is
ISE SLOPE
63-3 Note low (2) please refer to " electrolyte device
abnormal (cross-conta maintenance " in the "user manual" for
mination detail.
rate (A) is
as (3) Please re-execute ISE calibration.
following:C
l：（1）
SLOPE＞
-25.0mv

（2）current
ISE

108
 calibration
result is not
updated

（1）the
concentratio
n of
internal
solution（C
（IS））is
within (1) Enter into system maintenance, and
following execute "ISE check."
ISE the
range :Na：
concentration (2) please refer to " electrolyte device
64-1 Note of internal C（IS）＜
120.0mmol/ maintenance " in the "user manual" for
solution detail.
abnormity l or
190.0mmol/
(3) Please re-execute ISE calibration.
＜C（IS）

（2）current
ISE
calibration
result is not
updated

（1）the
concentratio
n of
internal
solution（C
（IS））is (1) Enter into system maintenance, and
within execute "ISE check."
following
ISE the range .K：C (2) please refer to " electrolyte device
concentration （IS）＜ maintenance " in the "user manual" for
64-2 Note of internal 3.0mmol/l detail.
solution or 8.0mmol/
abnormity ＜C（IS）
(3) Please re-execute ISE calibration.
（2）current
IS
calibration
result is not
updated

（1）the (1) Enter into system maintenance, and

ISE the concentratio execute "ISE check."
64-3 Note concentration n of
of internal internal
solution (2) please refer to " electrolyte device
solution（C

109
abnormity （IS））is maintenance " in the "user manual" for
within detail.
following
range Cl：C
(3) Please re-execute ISE calibration.
（IS）＜
80.0mmol/l
or
140.0mmol/
＜C（IS）

（2）current
ISE
calibration
result is not
updated

Because of
the
implementa
tion of the
ISE
maintenanc
e (ISE
ISE required
cleaning, Executive ISE calibration
calibration
complete
cleaning), it
is necessary
to
implement
ISE
calibration

110
Alarm Detailed
Description Solution
Code description

Malfunction 1： pump stops or fails to reach the top

solution：

observe running status of pump:

1.when working, injection pump is not running.

(1) Check whether both ends of connector of the motor

are connected well.

(2) Check whether the conductivity of motor lead wire

is good.

(3)check motor drive circuit of circuit board

18-1 2. when working, injection pump is running：

(1) check light sensor

SIP
SIP injection
injection (2) Check whether the conductivity of light sensor to
pump fails to
pump adapter and ISE circuit board is good and both ends of
reach the top
abnormal connector are connected well.

(3) check light sensor signal

check voltage between P901A plug pin 1，3 (measured

when plug it into plug seat), if the voltage is 5v, check
potential (measured when plug it into plug seat)of
P901A plug pin 2，3; The potential is high when
reaching to the zero position(discharging liquid),
otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light sensor
to adapter and ISE circuit board is good; if not normal,
replace the light sensor

If the voltage is not 5v, check voltage between P190

plug pin 1，3; if voltage is 5v, replace light sensor
adapter. If the voltage is not 5v, check input circuit of
ISE circuit board.

111
Alarm Detailed
Description Solution
Code description

Malfunction 1： injection pump stops or fails to

leave the top

solution：

observe running status of pump

1.when working, injection pump is not running.

(1) Check whether both ends of connector of the

motor are connected well.

(2) Check whether the conductivity of motor lead

wire is good.

(3)check motor drive circuit of circuit board

2. when working, injection pump is running：

(1) check light sensor

SIP
18-2 SIP injection (2) Check whether the conductivity of light sensor to
injection
pump fails to adapter and ISE circuit board is good and both ends of
pump
leave the top connector are connected well.
abnormity
(3) check light sensor signal

check voltage between P901A plug pin 2，3 (measured

when plug it into plug seat), if the voltage is 5v, check
potential (measured when plug it into plug seat)of
P901A plug pin 2，3; The potential is high when
reaching to the zero position(discharging liquid),
otherwise the potential is low. If the potential is
normal, Check whether the conductivity of light sensor
to adapter and ISE circuit board is good; if not normal,
replace the light sensor

If the voltage is not 5v, check voltage between P190

plug pin 1，3; if voltage is 5v, replace light sensor
adapter. If the voltage is not 5v, check input circuit of
ISE circuit board.

112
Alar
Detailed
m Description Solution
description
Code

Malfunction 1： Motor stops or fails to leave the top

(1) Check whether both ends of connector of the

motor are connected well.

(2) Check whether the conductivity of the motor lead

wire is good.

(3)check motor drive circuit of sample reagent disk

circuit board

(4) Inspect whether the rise and fall zero light sensor
is working well.

Malfunction 2：The motor swing to the required

position, but the signal is not detected by the light
sensor.
16-1
Solution：Manually enable the unit repeatedly rise to
top, transfer the multimeter to DC, negative points, 3rd
The nozzle of SIP nozzle core of plug P142, positive point ,2nd core of P142
electrolyte SIP fails to reach plug.
abnormal the bottom
1.If the value of multimeter changing between 5 V
–OV, the light sensor and the wire is working
normally.

（1）Check the wire

Check whether the conductivity of probe light sensor

（P142）to wire J2 of ISE circuit board is good and
both ends of connector are connected well.

（2）Check the light sensor input circuit of sample

reagent disk circuit board.

2. The value of multimeter is not changing.

（1）Check the wire

Check whether the 1.2.3 core of P142 plug is

connected well, and whether the two ends connected
well.

（2）Check light sensor signal

113
 Check voltage between P142A plug pin 1，3, if the
voltage is not 5v, check the connection of plug. If the
voltage is 5v, check potential of P142 plug pin 2. The
potential is high when reaching to the top position,
otherwise the potential is low. If not normal, check
the light sensor

Alar
Detailed
m Description Solution
description
Code

Malfunction 1：The motor stops or fails to leave the

bottom.

(1) Check whether both ends of connector of the

motor are connected well.

(2) Check whether the conductivity of the motor lead

wire is good.

(3)check motor drive circuit of sample reagent disk

circuit board

16-2 (4) Inspect whether the rise and fall zero light sensor
The nozzle of SIP nozzle is working well.
electrolyte SIP fails to reach
Malfunction 2：The motor swing to the required
abnormal the top
position, but the signal is not detected by the light
sensor.

Solution：Manually enable the unit repeatedly rise to

top, transfer the multimeter to DC, negative points, 3rd
core of plug P142, positive point ,2nd core of P142
plug.

1.If the value of multimeter changing between 5 V

–OV, the light sensor and the wire is working
normally.

（1）Check the wire

Check whether the conductivity of probe light sensor

（P142）to wire J2 of ISE circuit board is good and
both ends of connector are connected well.

114
 （2）Check the light sensor input circuit of sample
reagent disk circuit board.

2. The value of multimeter is not changing.

（1）Check the wire

Check whether the 1.2.3 core of P142 plug is

connected well, and whether the two ends connected
well.

（2）Check light sensor signal

Check voltage between P142A plug pin 1，3, if the

voltage is not 5v, check the connection of plug. If the
voltage is 5v, check potential of P142 plug pin 2. The
potential is high when reaching to the top position,
otherwise the potential is low. If not normal, check
the light sensor.

9.16 Resetting and other failure analysis

Alar
m Detailed
Code Description Solution
description

Time
synchronizatio Sending time
143 Check the communications of control board and
n synchronization
-1 sub-boards respectively.
order failure
failure

Check whether the water supply machine, magnetic

Water tank
valve, pipeline and filter are working normally, and
liquid system
143 Water adding check whether the water supply pipe has air, and
malfunction,
-2 overtime error check whether the floater is normal, and check
water adding
whether relevant electrical units of floater and
overtime error
control board are normal.

AD board Use control panel monitoring software to monitor

143 AD board detects the fault information to see if there is AD
-3 reset failure malfunction communication error. Repair AD board if there is
when resetting, AD communication error to debug.
AD board reset

115
 failure.

Reaction disk
detects
143 Reaction disk malfunction Lower machine monitoring software monitors error
-4 reset failure when resetting, information, and analyze the reaction disk
Reaction disk board-related failure to eliminate the error
reset failure

Sample&
reagent disk
detects Use lower machine monitoring software to monitor
Sample& error information, and refer to the related
143 malfunction
reagent disk information of sample&reagent disk board to debug.
-5 when resetting,
reset failure
sample&
reagent disk
fails to reset

Check whether the indication of reaction bath liquid

Water Water level detector is right; check whether there is
143 discharging discharging remaining water in reaction bath, and check reaction
-8 failure of failure of bath water outlet pipeline if there is remaining water
reaction bath reaction bath in it; check whether relevant electrical units of
liquid level detection and control board are normal.

Connect the main control board debugging program

Sample&
to electrify the machine; observe machine
reagent probe
electrifying flow; observe whether the 6 times the
Adding fails to add
143 normal cleansing movement of detergents reagent is
detergent detergent
-9 finished in the phase of adding detergents.
overtime error completely in
Corresponding control board of reagent&sample
the specified
probe should be repaired if the reagent&sample
time
probe did not achieve normal movement.

fails to detect
liquid level
143 liquid level
detection Refer to liquid detection error analysis to debug
-10 when adding
failure
detergent

Check the liquid level of reaction bath, and check

Liquid level
Liquid level whether the liquid level detector of reaction bath
143 detection
detection failure works normally; check whether relevant electrical
-12 failure of
of reaction bath units of liquid level detection and control board are
reaction bath
normal.

116
 Check whether the liquid level detector of reaction
bath is clean, whether there is water in reaction bath,
Reaction bath Reaction bath whether water level reaches the position where the
143
liquid level liquid level detector can detect, and check liquid level detector
-13
malfunction malfunction in reaction bath is working normally; check whether
relevant electrical units of liquid level detection and
control board are normal.

Bar code Bar code Use lower machine monitoring software to monitor
143
scanning scanning error information, and refer to the related error
-14
overtime error overtime error information of reagent&sample disks to debug.

Execute the main board debugging program to

monitor, and re-execute pipeline air exhausting and
observe whether air exhausting is carried out by
Pipeline
143 Pipeline exhaust reagent and sample injection pumps and the
exhaust
-15 overtime error implementation is complete. Use lower machine
overtime error
monitoring software to monitor error information,
and refer to the related error information of reagent
&sample disks to debug.

sample sample Use lower machine monitoring software to monitor

143 &reaction disk error information, and refer to the related error
&reaction disk
-16 start failure information of reaction disk to debug.
start failure

Use lower machine monitoring software to monitor

143 Reaction disk Reaction disk
error information, and refer to the related error
-17 stop failure stop failure
information of reaction disk to debug.

Maintenance operation of the sample&reagent

Sample& Sample& probe. Check whether the pressure sensor and
143
reagent probe reagent probe magnetic valve of sample&reagent probe pipeline
-18
blocked blocked are normal; check whether relevant electrical units
of pressure detection and control board are normal.

Previous Use lower machine monitoring software to monitor

143 Previous sample
sample adding error information, and refer to the related error
-19 adding failure
failure information of sample disk to debug.

Previous Previous Use lower machine monitoring software to monitor

143 reagent adding error information, and refer to the related error
reagent adding
-20 failure information of reagent to debug.
failure

143 Previous Previous stirring Use lower machine monitoring software to monitor
-24 stirringfailure failure error information, and refer to the related error

117
 information of reaction disk to debug.

Check whether waste liquid bottle is full; check

143 Waste liquid Waste liquid whether the sensor in waste liquid bottle is normal;
-28 bottle full bottle full check whether relevant electrical units of the floater
in waste liquid bottle and control board are normal.
Switch
Check whether the floaters of high and low liquid
malfunction,
level and the signal cable connecting floater to main
143 high-level
Floater switch control board are normal; check whether relevant
-29 floater detects
error electrical units of floater detection and control board
the signal, but
are normal.
low-level floater
fails.
Reagent level Reagent level Use lower machine monitoring software to monitor
143 scanning error information, and refer to the related error
scanning
-30 overtime error information of reagent board to debug.
overtime error

Check whether the vacuum pump and vacuum

Vacuum Pump pump switch are normal; check whether relevant
143 Vacuum Pump
negative electrical units of vacuum pump switch and control
-31 Failure
pressure low board are normal.

Sample
Sample barcode
barcode Use lower machine monitoring software to monitor
143 scanning
error information, and refer to the related error
scanning
-32 overtime in
information of sample board to debug.
overtime in
testing
testing

ISE detects
Observe whether there is ISE alarm information;
malfunction in
143 ISE reset
resetting;
Use lower machine monitoring software to monitor
-33 failure
resetting
error information, and refer to the related error
information of ISE board to debug.
failed.

ISE check ISE check

Observe whether there is ISE alarm information;
143 maintenance maintenance
Use lower machine monitoring software to monitor
-34 movement movement
error information, and refer to the related error
overtime overtime
information of ISE board to debug.

ISE pipeline ISE pipeline Observe whether there is ISE alarm information;
143 rinsing is over Use lower machine monitoring software to monitor
rinsing
-35 specified time error information, and refer to the related error
overtime
information of ISE board to debug.

118
 ISE malfunction
ISE Observe whether there is ISE alarm information;
143 when testing,
Use lower machine monitoring software to monitor
malfunction in
-36 follow-up ISE
error information, and refer to the related error
testing
stops
information of ISE board to debug.

Check gear pump and pressure sensor of sample

pipeline are normal. Check whether the read value
of pressure sensor which is running is more than
143 Gear pump Gear pump
2500 by using main control board debugging
-37 failure pressure low
software; check whether relevant electrical units of
pressure sensor and control board are normal.

Sending reagent
Sending
mapping
reagent
143 information
Refer to instrument module error analysis
mapping
-42 failure, adding
information
reagent may
failure
fail.

When the water

tank
temperature is
over 36.5
degrees, add
Execute the main board debugging program to
cold water into
observe whether the display of temperature is
the tank into to
normal and room temperature is high; check the
143 cooling water cool down.
water supply pipeline of water tank is normal; check
-43 overtime Temperature
whether relevant electrical units of temperature
could not drop
sensor and control board are normal.
to 35.5 degrees
in one minute,
which means
cold water
temperature is
too high.

Execute the main board debugging program to

Analyzer Malfunction monitor whether the communications of main board
143 among and sub-boards are normal; check whether relevant
module
-44 modules electrical units of sub-boards communications and
malfunction
control board are normal.

119
 Check whether light source, water quality of
Continuous incubation bath, reaction cup and counting light
Continuous
143 emergence of 5 sensor of reaction disk are normal; test data
emergence of
-45 dirty cups collecting board lines by using the testing program
dirty cups
of data collecting board.

AD data AD data Execute the main board debugging program to

malfunction mixed monitor whether the communications of main
143 control board and AD board is normal, and analyze
-46 error; check whether counting light sensor is
normal.

IES ISE measuring Use lower machine monitoring software to monitor

143 malfunction in internal error information, and refer to the related error
-47 testing standard liquid information of ISE board to debug.
failure

Execute the main board debugging program to

Version
Version number monitor whether the communications is normal, and
143 number
reading analyze error; check whether relevant electrical
-48 reading
overtime units of sub-boards communications and control
overtime
board are normal.

9.17 Refrigeration system

Alar
m Detailed
Code Description Solution
description

(1)check whether sample&reagent disk cover is

covered, whether the ambient temperature is in line
with environmental requirements of instrument.
refrigeration refrigeration
(2) observe the temperature displayed on digital pipe
144-1 system time
of refrigeration system and Peltier current value are
abnormal abnormal
normal.

If abnormal: Please check Peltier and refrigeration

circuit board

120
 （1）observe whether current value displayed on
digital pipe of refrigeration system is normal.
refrigeration refrigeration
144-2 system current
（2）Make sure which current is abnormal, and cope
abnormal abnormal
with it after check the corresponding abnormal Peltier
and circuit board.

refrigeration refrigeration （1）observe which chip is abnormal

144-3 system chip
abnormal abnormal （2）cope with the corresponding abnormal chip

（1）check the communications wiring of refrigeration

board and main board

（2）check the communications interface circuit of

refrigeration refrigeration
refrigeration board
144-5 communicatio status
n abnormal abnormal
（3）check the communications interface circuit of
main board

The 1st line The 1st line Check the 1st line refrigeration Peltier and cooling
refrigeration refrigeration chip
145-1
chip current <5A
malfunction

The 2nd line The 2nd Check the 2nd line refrigeration Peltier and
refrigeration line refrigeration chip
145-2
chip refrigeration
malfunction current <5A

9.18 AD Collector
Alarm Detailed
Description Solution
Code description

The 1st line The Check the AD collection board and preamp board
146-1 AD collector measuring
value of the

121
 malfunction 1st line AD
collector is
over normal
range

The 2nd line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-2 2nd line AD
collector is
over normal
range

The 3rd line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-3 3rd line AD
collector is
over normal
range

The 4th line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-4 4th line AD
collector is
over normal
range

The fifth line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-5 fifth line AD
collector is
over normal
range

The sixth line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-6 sixth line AD
collector is
over normal
range

122
 The seventh The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-7 malfunction seventh line
AD collector
is over
normal range

The eighth The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-8 malfunction eighth line
AD collector
is over
normal range

The ninth The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-9 malfunction ninth line AD
collector is
over normal
range

The tenth The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-10 malfunction tenth line
AD collector
is over
normal range

The 11th line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-11 11th line AD
collector is
over normal
range

The 12th line The Check the AD collection board and preamp board
AD collector measuring
146-12 malfunction value of the
12th line AD
collector is

123
over normal
range

124