Computer Assisted

Semen Analysis

Touch smart Soft/Hard copy Wi-Fi Tablet Bar-Code Integrated Sperm

interface Reports Support Support Chemistry Analyzer
 Spermolyzer

Computer Assisted Semen Analysis System utilizes modern computer and advanced image
processing techniques for clinical test of sperm motility, quality, morphology, vitality, DNA
fragmentation, HOS and nuclear protein. Based on the sperm test standards of the WHO 5th
criteria (2010), the system can analyze the characteristic of sperm comprehensively through
image processing of sperms. The system analyzes these values and generates accurate
parameters to reflect sperms’ quality. The whole procedure is fast and provides several important
NATURALLY SHINE information that are vital to present scientific basis for the male reproductive ability.
 Creative Constant-Temperature Operation Desk
The operation desk makes provides a constant temperature of 37 during sperm testing during the whole test
Standard Methods: procedure. This in turn eliminates the influence of excessive low outside temperature on the tested sperm such
as sperm velocity, sperm vitality etc.
• Spermolyzer includes two standard methods ( Dynamic and Morphology)  
• The analysis of the parameters is performed according to the recommendations given by The Unique Virtual Grid Function
world health organization (WHO) for the latest 5th edition for motility patterns & Morphometric This function has realized the direct comparison of test results between human being and machine in the
system. If you wish to use manual counting there is no need to buy an expensive calibrated counting chamber,
assessment of human semen.
it is all in your screen with the aid of virtual grid function

Database:
• The in-built database enable the user to store, backup and restore any kind Of obtained information .
1- Dynamic standard method : • The in-built database enable the user to generate historical report for any Patient.
• The method is designed to estimate the spermatozoa concentration and their motility in a native specimen. • The in-built database is capable of helping researcher by generating many Statistics reports.
• Determining spermatozoa concentration in ( million/ml)
• Measuring spermatozoa movement parameters and classify this movement to classes in accordance of WHO Other Features of Spermolyzer software:
recommendations . • Spermolyzer software is able to automatically add appropriate comments according to the patient results.
• Measuring spermatozoa dynamic parameters ( VCL/VSL/VAP/MAD/ALH/BCF/LIN/WOB/STR) • Spermolyzer software has the ability to generate accustom reports like Urine, Stool, CBC and Bone marrow
with any captured image using the system digital camera and microscope.
2- Morphology standard method: •Spermolyzer software allows the user to record and print the manual test result of semen.
• The method is designed to determine the morphological parameters of Spermatozoa.
• The software calculate the ( TZI/SDI/MAI) according to the latest criteria Of the world health organization.

3- Vitality test:
This test is important to determine if the non-motile spermatozoa are alive or dead.
It should be assessed if a low percentage of sperm are progressively motile, e.g., 30 - 40%.
The two most common methods of sperm vitality assessment:
• Eosin-nigrosin staining.
System Requirements
• The hypo-osmotic swelling test ( HOS).

4- Sperm DNA Fragmentation Test:

This test provides a reliable analysis of sperm DNA integrity that may help to identify men who are at risk of
failing to initiate a healthy ongoing pregnancy. Information about sperm DNA integrity may help in the clinical Computer Desktop, Laptop or All in one PC
diagnosis, management and treatment of male infertility and may be of prognostic value in assessing outcome
of assisted conception treatment. Operating system Windows 7 or 8.1 (32 or 64 bits)

5- HOS: Processor Intel core i3 or Higher

The hypo-osmotic swelling test is based on the semi-permeability of the intact cell membrane, which causes
HDD 1 TB or Higher
spermatozoa to “swell” under hypo-osmotic conditions
All the curled tail sperm are labeled as ‘Live Sperm’. The damaged / broken
Ram 4 GB DDR3 or Higher
sperm membrane, allows fluids to pass across the membrane without any
accumulation & hence there is No Cytoplasmic Swelling & the tail remains straight
Display Adapter ntel Integrated graphics or ATi or nvidia 1GB
(No Curling). All these sperm are labeled as the Dead sperm.
Camera USB 3.0 industrial CMOS digital camera 30 frame /sec
6- Nuclear Protein Assay: 640 x 480 minimum resolution
The mature chromatin contains around 85% Protamine & 15% Histone, where Olympus, Micros, Nikon, Zeiss, Leica C-mount 1x,
as immature chromatin contains more than 15% Histone. Microscope Trinocular tube 1x,
Objective : 10x, 20x, 40x, 100x oil plan achromatic lens.
Increased levels of persistent Histone in turn indicate a break in the development sequence of Histone – Transition
Heater Special temperature controller with attached heater
Protein – Protamines, which significantly affect the DNA chain folding & vulnerability for increased DNA
on the microscope stage
Fragmentation & this leads to decline in sperm fertilizing potential. Hence by evaluating Nucleoprotein assessment
the diagnosis & prognosis of Male Infertility can be determined. Slide Special Slide for count and motility
 MIRALAB ANDROLOGY LINE

Andrology lab will be most Easy and Practical….

With specially developed instruments & accessories for all sperm function tests considering their specific
functional and ergonomical needs.
The instruments are developed considering in vivo temperature environment of the natural processes,
Where ever needed which brings systematic approach and give quality test results.

Spermolyzer (Smart CASA system):

Spermolyzer is a computer assisted semen analysis System which utilizes Sperm
modern computer and advanced image processing techniques for Vitality
clinical test of sperm based on the standards of the WHO 5th strict criteria. Test
Sperm Viscosity
Dynamic Reagnent
Spermolyzer Software support: Test Kit
DNA Sperm
* Sperm Dynamic Test. * Sperm Morphology Test. fragmentation
Sperm
Morphology Fructose Kit
* DNA fragmentation Test. * HOS Test. Test Kit
* Sperm Vitality Test.
Spermolyzer
(Smart CASA
system)
Sperm
Sperm
Morphology
Function Sperm
Test Test Kit Vitality
Kit
Warmer for Semen specimen:
Semen specimen warmer is a temperature control system which is
ergonomically designed to give ease of work and specimen handling. HOS
The system has heater & sensor programmed to attain & maintain 37°C. Test Sperm
It has very high accuracy with ± 0.20C. Sperm pH strips
HOS
reagent
Kit

Non-
Spermicidal Zink
semen determination
Warmer for DNA fragmentation Test.: collection kits
pouch
DNA fragmentation test warmer is a dual temperature control system, designed to
expose the agarose tubes at 70°C or at 37°C in separate blocks of instrument. The
two blocks for two different temperatures can be used simultaneously. Semen Andrology Nuclear
You can thereby cut off the hassle included in conventional method and do the processing DNA
work in ease in just one instrument. fragmentation
Solution protein
assessment
Also, Its ergonomics allows user to handle each tube without disturbing others.
solutions reagent Kit Kit kits
IUI
Catheter.

Sperm Centrifuge 37°C: Medium

for sperm
Leukocytes Antisperm
antibody
Confirmation
Sperm centrifuge machine designed to work at 37°C, that is same as the Medium
Swim up (IgG & IgA)
technique kits Reagent
in-vivo temperature, to maintain the natural functionality of sperm. for sperm Kits
Swim down
technique
Technical specifications:
–
•Maximum speed: 4000 RPM (with •Maximum sample volume: 60 ml. Glycosidase
rotor 4x 15ml.) •Speed setting & display: Digital. kits
•Maximum RCF: 2,719 X g (with rotor •Speed accuracy :± 100RPM.
4x 15ml.)
 HOSPITEX HOSPITEX HOSPITEX HOSPITEX
Tel:+202 2504 4134 Tel:+202 2504 4134
Diagnostics
Fax:+202 2504 4135 Diagnostics Diagnostics
Fax:+202 2504 4135 Diagnostics
Email:sales@mira-lab.com www.mira-lab.com Email:sales@mira-lab.com www.mira-lab.com

Semen Screen (Computer Assisted Semen Analysis) Semen Screen (Computer Assisted Semen Analysis)
The system follows WHO (2010) strict criteria for motility patterns & morphometric assessment of human semen.
Motility Report
Name Patient Name Case no. 201502251
Age 23 Date 25/02/2015 10:31:13 ‫ﺹ‬ Name Patient Name Case no. 201502251

Lab ID 2 Ref. by Dr.\Galal Ibrahim

Physical Proerties Dynamic parameters (I)
Abestination 4 2 - 5 days _ Classification Conc. (million/ml) Total number (million) Percentage %
100
Collection Masturbation at lab Tested sperms 54.15 216.6 100
80 _
Volume 4 >= 1.5 ml Total motility (PR+NP) 48.66 194.64 89.86
pH 7.7 >= 7.2 60 _ 54.38 Progressive motility (PR) 29.45 117.8 54.38
Color Grey Opalescent Non progressive (NP) 19.21 76.84 35.48
40 _ 35.48

Semen Screen (Computer Assisted Semen Analysis)

Odor Normal Immotile (IM) 5.49 21.96 10.14
20 _

Semen Screen (Computer Assisted Semen Analysis)

Viscosity Normal 10.14
Progressive motility (PR) Spermatozoa moving actively either linearly or in a large circle regardless of speed
Liquefaction time 20 15 - 60 min. 0
PR NP IM
Liquefaction state Complete All other patterns of motility with absence of progression, i.e. swimming in small circle, the flagellar
Non-progressive motility (NP)
force hardly displacing the head, or when only a flagellar beat can be observed.
Test Result Immotile (IM) No movement
Test Result Status Reference value
Concentration 54.15 Passed >= 15 (million/ml) Dynamic parameters (II)
Total sperm count 216.6 Passed >= 39 (million/ejaculate) VCL (µm/s) 32.44 VSL (µm/s) 16.61 VAP (µm/s) 23.33
Progressive motility (PR) 54.38 Passed >= 32 % DCL (µm) 26.49 DSL (µm) 13.53 DAP (µm) 19.04
Total motility (PR+NP) 89.86 Passed >= 40 % MAD (°) 57.57 ALH (µm) 2.99 BCF (Hz) 2.93
Morphology index 24 Passed >= 4 % LIN (%) 51.21 WOB (%) 71.91 STR (%) 71.05
Vaitality 63 Passed >= 58 %
VCL Curvilinear velocity VSL Straight line velocity VAP Average path velocity
Agglutination & Aggregation
DCL Distance curved line DSL Distance straight line DAP Distance average path
Agglutination Head to head
MAD Mean angular degree ALH Amplitude of lateral head displacement BCF Beat-cross frequency
Agglutination grade Isolated ( < 10 spermatozoa per agglutinate, many free spermatozoa )
LIN Linearity (VSL/VCL) WOB Wobble (VAP/VCL) STR Straightness (VSL/VAP)
Non-specific aggregation Between active sperm and mucus thread

Other Cells Pinheads : 1 million/ml

White blood cells 2-4 < 5 /H.P.F. Sperm tracks and velocities Legend
Pinheads sperms are not counted as spermatozoa or
VSL
Red blood cells 4-6 < 5 /H.P.F. head defects because they possess no chromatin or
head structure anterior to the basal plate. VCL
Spermatogenic cells 1 /H.P.F.
VAP
Epithelial cells 1-2 /H.P.F. ALH

Normal seminal profile MAD

Head displacement
The semen passed the W.H.O. strict criteria for count, motility and morphology.
Comment

Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Italy Italy
 HOSPITEX
Tel:+202 2504 4134
HOSPITEX HOSPITEX
Tel:+202 2504 4134
HOSPITEX
Diagnostics
Fax:+202 2504 4135 Diagnostics Diagnostics
Fax:+202 2504 4135 Diagnostics
Email:sales@mira-lab.com www.mira-lab.com Email:sales@mira-lab.com www.mira-lab.com

Semen Screen (Computer Assisted Semen Analysis) Semen Screen (Computer Assisted Semen Analysis)
Charts Report Morphology Report
Name Patient Name Case no. 201502251 Name Patient Name Case no. 201502251

Progressive velocity (VSL) Track speed (VCL) Normal sperms (Morphology Index) 24 % Terato sperms 76 %

% % A. Head abnormalities C. Principal piece (Tail) abnormalities

100 _ 100 _ Normal
Big headsperms (Morphology Index) 32 % Normal
Short tail
sperms (Morphology Index) 0 %
Teratohead
Small sperms 4 % Terato
Bent tail
sperms 0 %
80 _ 80 _
Tapered head (Morphology Index)
Normal sperms 0 % Normal
Multi tail
sperms (Morphology Index) 0 %
60 _ 51.18 60 _
Pyriform head
lblPyriformH 0 % Terato sperms
Break-down tail 0 %
40 _ 40 _ 34.60 Normalhead
Round sperms (Morphology Index) 0 % Normaltail
Coiled sperms (Morphology Index) 0 %
21.98
20 _ 20 _ 12.53 14.01
17.03 Terato sperms
Amorphous 4 % Terato sperms
Irregualr tail 0 %
10.44 10.20 9.06

Semen Screen (Computer Assisted Semen Analysis)

7.13

Semen Screen (Computer Assisted Semen Analysis)

4.95 5.64
0.55 0.69 Vaculated 0 %
0 µm/s 0 µm/s
0-10 10-20 20-30 30-40 40-50 50-60 >60 0-10 10-20 20-30 30-40 40-50 50-60 >60 Small acrosome 52 %
D. Excess residual cytoplasm (ERC)
E. R. C. 0 %
Double head 0 %
Path velocity (VAP) Sperm distribution
% % MAI (Multiple Anomalies Index) : 1.68
100 _ 100 _ B. Neck & Midpiece abnormalities The mean number of anomalies per abnormal spermatozoon

Normal
Thin neck
sperms (Morphology Index) 12 %
80 _ 80 _ TZI (Teratozoospermic Index) : 1.32
Teratoneck
Thick sperms 16 % Total number of defects divided by the number of abnormal sperms

60 _ 60 _ 54.38
Sharply bent (Morphology Index)
Normal sperms 8 %
SDI (Sperm Deformity Index) : 1.28
39.16
40 _ 40 _ 35.48 Asymmetrical
Terato spermsinsertion 0 % Total number of defects divided by the number of sperms counted

20 _ 15.25 15.79 14.94

10.60 20 _ 10.14 Class Head length Head width Length/Width (%) Head area Head perimeter Acrosome (%)
3.57 0.69
0 µm/s 0 Mean of normal sperms 4.48 3.05 1.49 12.53 12.89 37.71
0-10 10-20 20-30 30-40 40-50 50-60 >60 PR NP IM
Mean of terato sperms 5.43 3.05 2 14 15.77 177
Track image (I) Track image (II) Reference values 4 - 5.5 2.5 - 3.5 1.5 - 1.75 7.85 - 15.1 10.8 - 14.9 40 - 70

Head defects Neck & midpiece defects Tail defects

Tapered Pyriform Round Bent Asymm. insertion Short Bent

Amorphous Vaculated Small acrosome Thick Thin Coiled E.R.C.

PR (Progressive) NP (Non Progressive) IM (Immotile)

Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT

Italy Italy
 HOSPITEX
Tel:+202 2504 4134
HOSPITEX Tel:+202 2504 4134
HOSPITEX HOSPITEX
Diagnostics
Fax:+202 2504 4135 Diagnostics Diagnostics
Fax:+202 2504 4135 Diagnostics
Email:sales@mira-lab.com www.mira-lab.com Email:sales@mira-lab.com www.mira-lab.com

Semen Screen (Computer Assisted Semen Analysis) Semen Screen (Computer Assisted Semen Analysis)
Morphology Images Vitality Report

Name Patient Name Case no. 201502251 Name Patient Name Case no. 201502251

_
100

80 _
# %
HOSPITEX HOSPITEXLive sperms 4 44.44 60 _ 55.56
44.44
Dead sperms 5 55.56
Diagnostics Diagnostics 40 _

Semen Screen (Computer Assisted Semen Analysis)

Semen Screen (Computer Assisted Semen Analysis)

Total sperms 9 100
20 _

0
Live Dead

Semen Screen (Computer Assisted Semen Analysis)

Normal sperm Thin neck Small acrosome Normal sperm Thick neck Small acrosome
Small head
Thin neck
Morphology Images
Big head
Bent neck

Name Patient Name Case no. 201502251 Vitality image (I) Vitality image (II)

Semen Screen (Computer Assisted Semen Analysis)

Live Dead

Normal sperm Normal Sperm Small acrosome Normal sperm Thin neck Small acrosome Normal sperm Thick neck Small acrosome
Small head Big head
Thin neck Bent neck

Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT

Italy Italy
Tel:+202 2504 4134
HOSPITEX
Fax:+202 2504 4135 HOSPITEX
Diagnostics
Email:sales@mira-lab.com Diagnostics
www.mira-lab.com

Semen Screen (Computer Assisted Semen Analysis)

DNA Fragmentation Report
Name Patient Name Case no. 201502251

# %
DNA Fragmentation Index
Non fragmented 32 71.11
Fragmented 13 28.89
DFI 28.89 %
Total sperms 45 100 15 > DFI Excellent fertility potential.
15 < DFI < 30 Good fertility potential.
# % 30 < DFI Poor fertility potential.
Degraded 4 8.89
Without halo 9 20 Comment
Small halo 14 31.11

Semen Screen (Computer Assisted Semen Analysis)

Medium halo 17 37.78 The sample has a good fertility potential
Big halo 1 2.22

_ _
100 100

80 _ 80 _
71.11

60 _ 60 _

37.78
40 _ 31.11 40 _
28.89
20.00
20 _ 8.89 20 _
2.22
0 Halo size 0
Big Medium Small Without Degraded Non Fragmented Fragmented

DNA fragmentation image (I) DNA fragmentation image (II)

Non Fragmented Fragmented Degraded

Building No.6105/6 Quds St,Italy

Mokattam , Cairo, EGYPT
 Egypt & Middle East Head Office
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Tel : +202 2504 4134 / +201150222203 Fax : +202 2504 4135

Alexandria Branch Office

Building No4, Street No7 from El Naser St., Smouha, Alexandria, EGYPT
Tel : +2011 59 88 77 05

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