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Waters 2790 Alliance HPLC
The Waters 2790 Separations Module integrates a solvent management system and a
three-axis sample management system to provide high-throughput analysis
for HPLC, LC/MS, or flow injection-mass spectrometry applications.
When the 2790 is installed with a sample heater/cooler and a column heater, it is known
as the 2790XE. The sample management system in the 2790 uses an XYZ needle
assembly, up to four sample plates of up to 384 wells each, for a total capacity of 1536
samples. The 2790 uses a variety of plate types including several that are preconfigured
Operating Modes
The 2790 Separations Module can operate in one of the following modes:
• Stand-alone HPLC system controller
• Component of an LC/MS system controlled by MassLynx™ software
As a stand-alone HPLC system controller, the 2790 manages both the solvent delivery
and sample management systems as well as controlling one or more detectors. The 2790
controls the sample plates using a rotating carrier. Optionally, the 2790 can also control a
three-column, six-column, or column regeneration selection valve.
An LC/MS system that includes the 2790 and the Waters ZMD mass spectrometer is
designated as a Waters Alliance HT LC/MS System. For further information on
MassLynx control of the 2790, refer to the Waters Alliance LC/MS System Quick Start
Guide.
2790 Specifications:
Physical Specifications
• Height: 22.5 in. (57 cm)
• Depth: 23.5 in. (60 cm)
• Width: 23 in. (58 cm)
• Weight: 136 lb. (62 kg)
• Wetted Surface Materials: 316 stainless steel, UHMWPE, sapphire, ruby,
Tefzel® (ETFE), Teflon® (FEP and PTFE), PEEK, Fluoroloy G, Fluoroloy–
08R
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Environmental Specifications
• Operating Temperature 4 to 40° C
• Relative Humidity 20 to 80%, noncondensing
• Acoustic Noise <65 dB(A)
Solvent Management System Specifications
• Number of Solvents: One to four, three with vacuum degas
• Solvent Conditioning: Vacuum degas, three solvents plus purge solvent
• Typical Operating Flow Rate Range: 0.050 to 5.000 mL/min in 0.001-
mL/min increments
• Programmable Flow Rate Range: 0.000 and 0.010 to 10.000 mL/min in
0.001-mL/min increments
• Compressibility Compensation: Automatic and continuous
• System Delay Volume: <400 µL, independent of backpressure, 1 mL/min
• Plunger Seal Wash: Integral, active, programmable
• Gradient Profiles: Eleven (11) gradient curves [including linear, step (2
curves), concave (4 curves), and convex (4 curves)]
• Dry Prime/Wet Prime: Automatic, front panel control
• Flow Ramping: Time (0.01 to 30.00 min in 0.01-min increments) to reach
maximum flow rate
• Maximum Operating Pressure: 5000 psi (345 bar) at 0.010 to 3.000
mL/min; Programmable upper and lower limits
• Composition Range: 0.0 to 100.0% in 0.1% increments
• Composition Accuracy: ±0.5% absolute, independent of backpressure
(Proportioning Valve Pair Test [degassed methanol: methanol/propylparaben,
2 mL/min, 257 nm])
• A Composition Precision: <=0.15% RSD o r<=0.02 min SD, whichever is
greater, based on retention time. (Degassed 60% methanol:40% water Dial-a-
Mix, 1 mL/min, six replicates, phenone mix, 257 nm)
• Flow Precision: <=0.075% RSD or <=0.02 min SD, whichever is greater,
based on retention time (N = 6) or volumetric measures (0.200 to 5.000
mL/min), isocratic premix
• Flow Accuracy: <=1% or 10 µL/min, whichever is greater, (0.200 to 5.000
mL/ min), degassed methanol, at 600 psi backpressure
Sample Management System Specifications
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• Number of Sample Plates: Total of four plates:
- Microtiter plate – 96- or 384-well
- Vial plate – 2.0-mL vial (48)
- Tube plate – 0.5-mL microcentrifuge tube (48) or 1.5-mL microcentrifuge
tube (24)
- Open access plate – 2.0-mL vial (24 or 48)
• Sample Temperature Control: 4 to 40° C, programmable in 1° C
increments
• Maximum Sample Capacity: 1536 in four 384-well plates
• Number of Sample Injections: 1 to 99 injections per sample
• Sample Delivery Precision: <0.3% RSD, full loop 50 µL. (Default
wash/purge conditions, degassed 60% methanol: 40% water Dial-a-Mix, 1
mL/min, six replicates, paraben mix, 257 nm)
• Sample Volume Linearity: >0.999% correlation, 5 to 20 µL, partial fill in
50-µL loop, 3-µL pre- and post-sample air gaps (Default wash/purge
conditions, degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six
replicates, paraben mix, 257 nm)
• Needle Wash Solvents: Two: wash (strong solvent) and purge (sample-
compatible) solvent A
• Sample Carryover: <0.01% or <2.5 nL (default wash/purge volumes),
whichever is greater. (Blank injection, 25 µL, 60% methanol:40% water,
after six replicates, 25 µL propylparaben, 1 g/L, 257 nm)
Instrument Control Specifications
• Parallel Sample Processing: User-programmable next-sample aspiration
and loop fill, column equilibration, just-in-time gradient delivery
• Advanced Operations: Auto addition, sample pooling, open access, priority
samples
• Column Heater: 20° C (5° C above ambient) to 60° C, in 1° C increments
Proudly serving laboratories worldwide since 1979
CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment
IET - Used Lab Equipment - Refurbished Analytical Laboratory Instruments
