BLOOD > EE dees LA pS acceres SL oF bleoel “— Ayanspe LUNGS Jo THE TSNVES PON Sogety A- SP= Treat alt SRKS_ Ay be Pokeehialy mpechoys iP. U Keel all psa REAENTIAL SOUIEE_op OB paths BS X vecommend HANOWASKING oper gloves remeval B.HANDWASRING =mod egprchve Way F breaking the COl a) Wet heads £ witke 3 ety emp, Mechanicgl ep re ac @\coit 20 sea Bnasele ens ‘han ofp he faucet c. eegecnees jana wee OF deshoyng, gathoqcnic miodargarianr m ©S ouseholeh [yeaun (Wo bypochlew'e) Cad) CuK, daily) Phenat, teroslsidiol, 10% etnanel Steahzetion “Pesratchon oy ALL TORMS of miorSbial \ye ind. epores # Uame - cee x vee. discarding CoN Sie = 125-1310 Naod SPECIMEN COLLECnoNs Ne A.Nenpuactwe sora reserone ( en) on arenes RY 7 Order of DRAN EO ed pile v efsene CK, EDTA erhierivee oll cay" or SR WetCecrseD Food gases Sides : EDIA sion 4 NewbotAs, oryly Otter ane, Secu 1 Gp beg = Chsie for GD shat Hane - onkalycalr he & Scpowoten Gel - aS, some anaes) . cai P C MUSose , ee > switdn togfil B. Equipments a. Tourniquet) - 3-4 (7.5-40em) m. Gbove, LI min b. Collection Woes mock common CETS) a) Anigryedlyte aged - for Deval TESING (Gu) Not — €-Liiodvacelote Setoy gang 4 ea Restle Entel won) © so yp UREA & 1) Arphcoagulaed a. Sepa gel Ot ecm ‘aa | x 1a"t aes cNeedles a8 Peaiaic: DA ng jane ya iKenninn C1. /sien esac on + eas. C. News — 388 Gepralic= hatte, Cluge tm WN L Biachial artery Pc Gene | Ree et ” 7 Pied pe: Adal . we [ons 7 Latwcl side of We Prriteac 7 Eldely Px 2 Oe ga = pr ccthcaen 2 Bene ew ae het 4 Me ceesPopsitagic Sechacs (tem atom Dla 2 She 7 Sal, N es Po ~ CON 2 -2hvs ¢ > Ag = t es Po | ; = WRC count, Hab of = = “Hgb dik~ False T tioraia Act: by vernal cells —¥ EPO» Shmibotes REC prock. —» TREC, Tig KouTme Merely ~ RBC, WRC, Ho, WRC dipperenbar , REC indices « at 1) eg si p Bx fort) 4 ewe 4 lobia og, A heme 4 4aldoin drag &" Arve Cel fyetede) sa ct fhe 4reatment OF anemic comer ~< Bic? 15-20 gla Adalt warnery = (9 Adalt men: 13 - “ib gla M chattion St “feel Peshee sy wee # o starding "Seen remus a oduy: 1 Heb «Rolie: TEPO- TREC gd ‘ robkias T. CYANMethemaglbin c Moatyied.J Reagents: \K cycnicle = = donates ae K Lemeycaile ~ Cynwer gest tear depoced Not Noticos tae Osa) Shofhens fon \5-*5 yi @ a6 ami see Inge Rec Lysi eases. s 's «easy sue pe racrol the my High, AY Rams wneacwtaexce pl a A Ayo coules tAachity Con COANE * Anwtwies false ¢ m No nee igo SZ Hqb C_ | Lipemic Bleoct fers) WE cA:720x)07E Hah PH ft: 7300x107 Remedy « ben Ane mut ose yeTL. Blood Og Capa Cosanctic Nom Syke rmuthod ) Thera ses -16 oF id" ie ony mL o- Og T. Blord Fe content : 00g Ho= O.4tg Fe W. IS metinca Gronwnrtic) - ‘git Is Si Saat wank win lB gece 15 geeg & deg Plight Lom T43-TS6 Z. Caloime + Aad ae maefial #10 g ~ Atay “oct: Nee - HEE is allal resistant BHOImAL Ho Pgwents 0) Carboxyhaly = Rab 1 CO - 200% greater appwity = ¥ Nansed 0, ~ Reversiere = Chomy Peak % @ 536 nm byMthueagli Feondined fo Fe" = Revercinte ener ya 630 an = DRUGS, Ohemi als, c) Suleltgt> _ Wrversible fpr lige | wag Latent amine § “hae. [avert 28 Se Green Chenrg /rederks) @ Bem 4G- 4 x tk Pa F ree: 30-46% igo Maks. 40-45% om 7 Micahematront = Tube is WS mm vf Ubore F I. Lonty, O05 LB s Bea banal “grains bepAnin Cronahcoog,) 5 louse aCe rtand “Rate Clog sn 5 KEDA = ret b leh wean a incenplelr aeafet tool =a hae +8 TRAPRED RAGA * ta Ia yinacke puke conte “an stunt 2 | Mone etemcing - SiS se T “ ' Chee et 5 Tu: dy Ay Strenunterss, ala 7 Mao hematront = time Conguming;slarege owt. oF eloocA thd nna ac L Rule o¢ 3: @c Vallichhy Cheek ce + Bormacyhc, ne Oren Raw 3s Ab Viet = 0-519 Hb /160 Vox 3=Hel + 2 \ tcl = (07,006 RBG 3) BaC cast am, Graer) NY: oe: pS tie a be bovns) 7 PN, Graer) 3 ag: G.0 xlb Ditubrg Fivids: Gower, Cale, NSS , Torcon atveng , Bethel, Hayem, Dace REC/L= Hoy Ce ANCE x DR DEY Gig “LEY Csxoc04) C20) OF # cc x1 KX AO (oCd 004 a) whCct we preset aa Che Shas wing em §] in. fay (xt afc Crys) “i ins Alloa diviag to of ter LOsnins perpotinad whe 3 ae Ding Fluids : 9h Ac, Ih Hcl, Turks WH Hope iay “BS » Qet 0 HRS Cwec = Lo = tage ae ose NeS Garniloaytes : wl egrenteol o° apenas LR ear gery ais . (ire oc hoa . ye “ MPP( Major Rosie 4. Basophil Dov writ gees ae cells Meas Marge shoe Soa ped Ay clas , qveund Glatc app DEPT Au except Lympho |*) ta aa (Pov 21 SOF tem ahr’ Fantoagts * .NonspeaTH < - Sheet 4D 3 Rand is maimed o¢ long m Cange Fhe tert Predominant: Wile SITL — Tote night « Hy pergeqmenty nunghils (C7BlobesS in ehltren + 20D cel ogg Chuvt nes IS LIKIOMe bo cen _ Fim 1) Manual wedge = Covers ‘ — a Comma | me Swen ditt Op WES = ee min ~ blood drop ~ Yarcly vsrel - 30-45 °/ 25-40* = 22 X22 men caverstip Guay : — = Film is 2/3 - 94 @ shide 2 Ang = finger shaped e Sa of teed dnp I - who holes / frvegulonkes oF Stonang ° «wg, We 7Romanowsky Stam: Byars aie) Coane : Stans RNG Cone be os re Ee at gens se OF F exaninahon CAD =Cross- gctonel /ecenallation - side tp side AOE Shae MRS See ean poy 1 ge men! ‘efPeves VENTS ve e “ Sebentene (Wooe Method ea iF Malano] ° eA 2 1 vod - 1) Spun geo a coon Race) 38) Wick Blond DET. yalenal 2) one COAT = only the BS icused 9) TWN BLOBD= “TID. Seo unt Wary bles a 1) Ne Yan omg ba MT Nodtelphaia_C ve Tie tes oP {pin oo - A; ira db, Som roe tecepe” - Taremaeocy tosis - PCY, \Sinaic hye, cut Sp ene Choma (SOAS > Toarmbocy hypeaia - 1. ppm, AA, Aryl. Goren dey 1 Posmay onentnetho . Platelets 4) PHASE Mlonscopy 2) Tokar METHOD - ~Brechev_ Cronkite * r heguly ees ~ Rees, 7 Ecker * ~ Reference method = WSe a Ticget mempscipe = WH ts dived w) 1"), Nig onc + Rees 7 Ecker chivent : - EDV Con covse oll. solcliiisis Ams cor he > BOB - smn anced by using Na abate os the he cok 7 No. Chalke - anticeog tag, L weuhply pit count Loy i + Formeldetngre - Qrecewatve ile nS OIE ails = hagphy weprci ine is - cn Ml aan 2 st T wee y Retiilocte count ae o- ty oth = IS Ahe JASt Awninortvnt. 7/6 BC ‘mRO 72-3 cos in ron CLL rte seed RNA Nor chan) i onetcs ese Och. oF fre BNI : = TP OM s X X procs RECs t Eee eearl ante oy. Wad # MTB sa (2:3ap 50 wel ak) L mainte @ AT: D=1O WAS 2. Count (000 RBCS nee O10. of $2 Toimpave verter, hone ole’ ab Coat He cher anearohes chal ager wim LOD + Be ee Soh ene bakes by ay ARC aie Jace Ieverca ROC T= Hee ached A op veh mn AL oF WB = O - Er “75 xi |e. age tons BY QRE = Low Het spe Forces elovcicl Cos WB contin Sewer RBCS) “BASSET lame 45 LAB, KR oF B- 57% Wy Covepensore drsnia a) RPV = Reni veleated rd pemobets = Cells Age grat Gi e, Pee Loay ths, Rebe count per. count X. repre Ning a pea eday In erent £ RO 73= Adeavae £2 = \nodeaunle5) ESR os ic am mg. eapwrae \nortased : Ratlears Kiet’, ek hr Aap es . Yeorened NAT ncaa, sepa Ihe Nag it sae celle Pheccyks = rel. iaulacg" : a Ariso Fenkatleae BORG) stana argte ge aed WEN ne cy . a cee eR we gas an €- Mtchonicel hic Eee 7 BE oy Sten 0/o 7 Kole 1: low tap Fait T : wilotaton, neat Weegten ve wiht 300 > Ie 1 am 0-20 no WO 2.5 =r bo 3 oan 0-Aam: Men O-200 O-106 rey yIpd -O OND? Waren EDTA 6-10: men, cmlaa: . & Nactok ame Si of LESR Jar Senn vt eEce ‘STAGES i e tation rate is sight Lag phaso- in te st 10 minutes rouleaux formation oocurs and sedimentation ®. Decantation phase: Sedmentaion occurs fora porod approximately 40 minute at a more rapid and ‘constant rate © Slow sedimentation. occurs at he last 10 minutes, RBCs accumulate atthe bottom of the tube SOURCE OF ERROR @. Overant icogulation wih EDTA causes a falsely ow ESR. b stan ‘ '{|ESR stands for more than 60 minutes, the results willbe falsely elevated, fhe testis timed for lass minutes, vail ow resis are oblane lo ome Yeneratre °c ateet eae 4. Tiling ofthe ESR tube «+ Wo renee to ean np 9 Koc ©. Bubbles inthe blood cau 1. Fibrin clots pres | Rea blood calle anemia | Clinical eonaiions ‘Cotagen vascular aseases Diabetes meatus En stage ronal fare Malignancy Menstruation Multiple myeloma«) Eesisiophal count WV: 50-350 x 10° /L i nia: Ns Vrucell Eosinophiha Aller ic (XN, ccllesis, easy. Bemiars + Vio moming St Siebel leap Roveng mush ree, ind, fonts ») a. Wyse sue Of SapnIn Tn the ‘Mest onrca\ 2) Suoar sea — + Scwrening, k Pun aceon wdc m6 ox “Seow Rasen ces fri ® COS ( ON, COs RL) * ciate Boop C4). Hemshysis 10) Aci Sedna tee eu mei Degabiniadrc blood) ogyes Peete sat vm SE [Wer abs Vw AND Le Foto L corny. of ae, 1) Malanal Smear -W/G Sloined smear % Capillary Blood “Thick : Det Thin: 1D wo N®: x ysis JVQGS enn Neves : elon By guus a th aS V20-% Hes ae * fas ve oe oir =Hhs =e Sc cals “Wo AC ARS conem Vortelanel ce dpe. SA vs SCT (lac) we binds 4p Ye AA on G™ GC. Green, white/ Reck /Pink color G+ gray t-—} HbA, mee OST % antes: ies Faw Neline SS PAR vg COSWT ws eg OE are RRs aba © in serve VPNA Res ore mare sonsitig tus C4) Hewolysis ny) GET measures BC abihty te Lali up Poi »/ hyp A ace: SHORE OFT Spnenayrs, HA , ddr cells Sey serecnr, Liver Any SCA, VDA, FRR, ee a vm, REC e PAD = 0.5°A ile (alum) QR Avors 14-29) one Ny: mex Weve 1 (pa ¥ OFT) lt Te gout \@ 19 20 2) TI I ‘ ve" a2 cn Phe roe Y Irypeand snes ~ tel nauk pha Plana dep. on speuies8) 18) ny + Ce. exbnativen\ \n hewnaghit 2) BD Reesaiee bead /RumplecLeece/ Twwniquet To emily (M) , 90 omltglF ‘Ahee © mi Retechion stn X teprnkd fen 7-4 da “Hime t takes Goon std. wowed 40 shoe brea te SI Ane Ph eo A Jin, 25 Vessel wok v VW der faetar ge Bieeeting dle ve “8 cm sick 4 min, 200 55 torr Ouett fetching 5 wins afer retace We) Leow Gag time = measwe all clas ty vag Syckn_{_ menih tepome, Vi: 5-13 ming °) (cotlabe) i) hidin Bh JN io (2-*| mins), v bieds acie wat \) Ny method ¥ Recagniion of Mast cull & Pass udler sere emt dD. ce Viv: B°G Vsenenng £00: COD Ie KV — lad | Crd? @C Back ppl “Forrraein”) ONY. G 0 eae yy) TOT a hh et «At Paissian BNE ; Fe contenining boc =. a J ents QV ae, HewAtowresy Ss V sidontic qunnes,?boclies b hemasidoniy . ty Re + Mesobtatic /Yolk sor thase JSsaing ALP Jim the Ne Ne = 14™ Day Vv DgF. CML fam Le ae = (ew * Exnbryonic ferns % Wo: J Ry, a ee Les Annes, he esha “a3 Uae Yd: Cra, PN, SCA, IM, PA, a “te + Fottland - 22 12% VAN: 30-135, i 1 9k, r& Tien capil “qn ee a = Liver Conayee site) unl Gp ae ces) Gta beg : 27/3" 0s Stains penvidaves in Crowle £ mano Lymphe : 4% mam Mone. SM mon J AMLLML fy. AL J Wok “4% mon. a Wor - 2a + 2% a) ges eal Ses 9) Machillags {Aeehac oace av AML E NIM AW . BNL de 1S * AME - 3:1 eat AS Vi@xghidmo's s/o (FAB MY) sage 18 3 hs ny NY ecomn, A/S L LA ~ beck patton # HDA 24128 0S Rox natal 9 Ay7 ad4aS “netnatop nus SproRic ecrormce “ 7 Growte Ran ADULT Wemnetnpoichc y sc 4. BM — a. Rr many (0-4 ys) Jv MU ge GL Onde yal dev. cet h) TRAP b-Nellew mawond J Hares cell leukemia tes v TRAP: fees + Rebngirss on - ceploring ved by yelw air cells [pera ee Wee 7 2 Ayimehte C8 cell malignancy) “Bavoeeany herds» Sten} - = aha pit ar seevectorrd denan,Linphe, ed 2 ors foe Bane - weticvan wedwwor, ae hers RPYs QUIP - Arndnhic pocesies ~ AlKT y Corrs of trinth ,f oepiy gw apply % $0 Ee aes 2 Cemnever a Olling - (es O-* ) ki! # Pithng- — Brie teas Bro" OS *& Mite. dex A coinita valve set ty esl. celly ved wmhsis, Sieg Seay TS *Monophykhe T° SPSC + Poyphyiche T- ~ ows) vmtuve SC * CLE: Tt cell, & cell, HK cell CoM Gomis , ey tony, Mons weg - CROCE, QuiescenAenTumes Wtsis - Cel\ division, 1 Nhhase = contloks of Gl Sy Sea cons nase - Crromasoine condense Ch) (ani FN, Cr, ; J ~ Cenberstones 2 0. PSIES Dantens LAs by Fill Ihaphase — = SEK AM Man A seg got C) mplvente: Ter 6) INF Ob) INE ot phase ~ Chavis 90. pile =" Cen divides = [Erytaroey k_melebslisen] = [REC Singers]: ') ENN patiway +) Eronpimablad onaenbie gycayhe 9. Wan N.C vaio “prin =9-32| = “Joh her are Caer’) = hig} = Gork Bive cyto ¥ - na Glin ona! bears +) vue el or 2) Basephilic Viomelbiast = Gewbye ghycolysts (hae “NC of Gl 7MADe > NADPH = Deep Be (18 age) = ed. ghim wtdvics Ne # Uct. level og Ugh symtheas yoo (Fe ser Fe") 3) Raychamatic = GGPD Acc. a t at oot ae Bie [howls gay ve Dane main Ledichele #iisie Nob fog Wh vyeductat " 9) OAtrodmpmte 0 Lichen Bopegat Seat ~ PYKNonc Nudeds = ogverate s” 2,3-DPG = Meaty, Hb ee < = RNA reroviven , Dink cv/huplevin Comer Wo wea - ¥% hvoleus , 1-@ %» eteal geile” \20_ DAYSa o) a) 4 Exyrcacy DP Excessive EV \\. ExWowascvler_. (mi -mediatd ) Se re = Spleen ( - Pin Ms ear bis ul 7 qason Lor uMlolingeg — Uablin = gly = red. gycoysic CATR pnd. CUbsetnen ul a - Trace Urobilinogen tr emiic, = Wn Shook 4) Excessive NA. owiehe Pins INS 7. ¥-® color - yi i- iowascor Vi ( Mechanical) wna, lonnunn * = Reghaghbin 2 Hemopacin Saheqe vel. Ho , = Albumin Komg. hilds metheme £ paucs tthe Wengen PY WOH? herialibinvner [ Hemogtsbinune APemondennutior TUdvbati nven WMittchonclnion P80 gpetorc Cyivae) Giyone Vane’ tua abyaatere Pl S.0A sh uae Poutaing 1 ree dame brydetng lbitane Dieter VPG m + URC cecarboreyon Prope hyeagen Cogro TE Femuddaine | znd pnibibien re Hen ene . Goer { - ret af Ye Br 8,£.¥ Wor - x+y a z A - a+b Av ~at$ 4 gqengt 055852 QL Gena: X,Y ¥@ wb amc m adlts 92-99 Io Cryor) 25 LZ Crean 1-22 Crier)mm Pag - awl. oF Og nacdech fe tabrmk Sob oF Ho (1F malig) Bohs epprot - sh out tothe ket [right AE ie sy Sogo Oy tet b v oe oeeerr T Oe dui. Across hye ¢ r etsy % Gaia ,[smtoge Shope >. -Ae PE eeIGE (ay-a5) Ne ~gfanvtes Cortpin ACP oad Wydeilane, c. Ni yt fnutomidase @lacte@rin pepytos Bee ee “Beds Ee auth Nighy incenied [1) shaped nucteus whit bact. qrowt : 7 MITOSIS. Clot stag capable) —buperseqiat'y : VA. Be “hey = Allww afore dapr 9. NEUTROPHIL PooLS 'BONE MARROW Metzner, Ban, Segneria Nevrgna_| FORATING POOL 2) Eosm) Eosmeohi| mal 2) “HR oe = Pgeanvtes Stam heanly o| € = De Bak qyandn. “tee pe? os Mi ¢. Mate =X ALP mm onl x Wad cele te - Lweek in BlosD ~ Gheredcleycten oogiel = Ceyenants apan(atl moh PKS) IT Tagory 29/7 Seeipic 3° a-Manablad ~Baighivc ante — [Rreenfelonh| PREY [Wictn band - WV rant = hae b2-m\or0, bb Rroamandayhe “Blue qvapr cy aes pl Be — a9 eae c \nimeayte ~ Phe a aie ss dohes) © ¥| a ae “an f= ie? eee Sd am 5) Lymphoask sat . 0 be wag si a aete ©) WBC mat: L Lifespan A Nevbophl: 2° days (min 2 A Mokoaye cin 4) ao 5 Clge 7TSSHE = Months, longer & EOS\Wophil : hs. ‘ss Sigeean 4 ee eiomts fod Mg cal & Basoelal : 4 (Mee) Myemphueg le: PS wy 4)» char ~ Anucleated - 150-450 xl07/L es Jor acnvateay : wre quar /SeieacAt ( alts, ~ ROUND CEDtA) ~ BICONVEY (Reshing pits) aw spine Leas : a ro BM: Niegakaryoayks: tite manmay ayenore Rati ADS ayer | Ta TE fuk) \ 20n¢ eajcocntyn, YM, Sdomeont ora, 1b, Gp2e5a pivedhctin WA ‘ante D. Sal “Uarivechir * \miomflaments CACTI reqasiny “Senet Imonveuress Cubvhin’ : retains ph, shape ¢. Orgamelle zane +"O-granvies = ~ (2) hemostasis + Dense qromwles = 1° hemostasis = eee wi oamay Ssee fusdetn pyr a ee end) atelet sthwdur & F. STRESS/retalated gis = Compenscthon for + TEs , (2-14 Cowy fret abo, Frogmeab o FER, 15 Wy REC rene. Fase Gare Carly /ropeh Prplt tendon enw “\mmatwe BRU-Nieg- ask mate ves CEU-Meg = Memecioke wi - "TOSS: X cyhhaines 2 ) Edom: Fait © Heep (x00), | ocr ne6 mana on hepabes ¢ : ol ic grands — yarns woes x Weg, syste: + Sep: gs Sto of Xn meg oultwrs viel EN Ah ye & roar mee Ke inal ‘onals vols POCCsSes: 4 — = Qe et NON Remy Se Pe KG Heer aire mek weltat (bes Ties st ey ta = tng a 7 anaes xs NPY is muta NF Count OTS akon eer t * SCES - pli. granule reine 4) Galhedlery - revonilok 2) Aggegation - \nrovensiphe =YtO. Alera DB) Sroretion - ~yeaibe oe = ER 8 comFibnnaly ¢ ‘Remostasis Blood flew A. F hemostasis - fumtn of gli. plug V Components Voisuilar gust ¢ pls a) Bend vessels : % Lavers, ¥intod 7 Andthmioic — prevents Prcwortwe cating, Thod coat WW ove: °. “6 Wooe - CHerorepeanh ‘prevents Qi. adhesion) Coats ends supace t weakly enlace AlriebyNe oct. % promote coagulation elect Be avo > Nasocanswichin, Laren gis) 7% > Exposed arti do) : DI. acthevence wes Grain, 7 Release in: miko, etieele pete takes Flonnalyhe pathwiewy Yeheasek ds ca RICYSANIA Metabshkt thal Imnioks EY. ochJathon OE rate = Nasollay™ relaxing" Foor Fe - Quasaminogyinn tral enhances onhthambin Iii ochinhy + SAninsic wey 1 sadhaic ok Bede aoe es SRE T cman NS ERR @ Themen ADEN AINE - Nasoailation , cavers BF wore = 7 4 -NWE- gor pit aac Pl api binds Fete ee ce TRA Ranmeqin TA plevmin acrvates FIBRINOLYS I 9). PAIL 2 TAED - preonis fxressive poumin sprees A Duving, pH. achivarton, Men. phospmlipias AEA AtaOnidenic AAP (Twat) ——Treombaione J. Cyeloonygenase [ASR N. reps ARE MOONS) Thembowaine Ap « IMME Pes Heong ase?} “has ows 1H Ctause veleaie oF Cole promalts ih. aggre £ vacocis. 2) Platelets - “fa Vey TRO ave Metrokane Consumes Qa™ 4 AP wa stot Wweethon of prin det, debulking of olst( endeets) ON = nitiaka by Sertatn £TxAz, On): atiednmnent te Cogn $ ame Comp. = agen) glais(invite) eal ran Seg sSheat Vatts : veavets WWE b orien” La cnet! cat ae te pi hay i cad mere = sie 1-2 mins , Passive * reac = te the J oF Ca” nediatoes : Sateen eee foe = Stetblized by Four“Factor Xml Prekallikrein __HMWK De Wemslaasis ~ COAG reer (eompunents) 0 ak Os . Proce sw ree Tomy alas that X ri become activo E 7 TIMOGE): &. preouisor /pmenrymes (MALIVE) (2947,10,1012) (age + Serice Proicases: Mla, Wer, Xo, Xie , la, Prefealli, Hanwlt e + Transqlutaminase: Killer (12) 7 COFACTET: eine wa aolivetion of Zymogen Core, teamed, «No cae IXov sMiel or lo 2 Xen + CDH) Bor achvatra cs 2 Va ger Xa VE Gee VM 8 Cron Cc 7 CALCIO, Preferred Name 7 Fibisoren Prothrombin Prikrombin ete Fate we onion Galelum Crmeca ese a roaccelerin ile factor ie @ toa fomip) Froaceslenin" OS UST ROLE ef lum ee) " Froconverin iSchcet arc yi WWE ( Chun of sock) Autoprothrombin | Aree ow Athemepiic kor) Rt v Ven ak Anilhemophiic ¥ Tatar (As) Pins espa cowpenea 770) Finte coat? domed Some x snarcriower ctor Sean cor = , ~~ People os asa iomibo eee ere ———— fra - WOVE ar fess tctor dias ctor Fallen ian Pan saing ae Saran ‘Piatra anagataminase Coty 46510 ——Prekalikrein Fetter for =i Highmolcular weight Kininogen —egwa factor vim Circulates in blood bound to von Willebrand factor (vWF) j Antihemophilic A factor (AHF) nai Factor Vill complex Factor vie ‘Coagulant property Measured by standard factor Vill assays, “— Markedly decreased in Hemophilia a —____ [Factor Witrag [Antigenic property Measured by immunoassays wir ‘Acts as a carrier for the VIC —_ © Responsible for normal bleeding time 9 ytelet. Aes Deweme adhesiveness in vitro on VWFIAg Previously termed as related antigen (FVi LWACEARIN) NF antics Measured by immunoassays er neretinior VWF:RCO Ristocetin co-factor activity PAVKAS (coors wtb a Regonuds Active Form Pathway Fibrin clot_ Common Serine protease Common Cofactor Common Serine protease Extrinsic Cofactor_ Intrinsic Serine protease Intrinsic Serine protease Common Serine protease Intrinsic Serine protease Intrinsic Fe Common Serine protease Intrinsic Serine protease Intrinsicvharmiisic Sg, ody &, Pre alli ANWE, Car, PL ? Nsic 3eommon 12, “a, la, PL Mechanism AS ° 4° 3) PIF adnesion B tt. se Bape o arecte | wut ase Me OE Care te BS slo - Xgp1B “atom ph. slo" 0. PW. eckvoman - viscous metamayenosis / rege ‘een 5 9. PW. Srorchon —X aqroniles + All othe (vere ~v Verse cline t poe Gee Caras Cashel “| aR OTE ~ otocrm ent we Collofila BRIN, PL expos = ste Re Abend A Abeemivacgaeis : * 7 moor 3,454 PE . Exinsic AWTRINSIC-(12 Wad sgt Contact C124) 3310.) Nessel myuy Ha cou TEEM fitkot wel Xt —r a. Intense Terr comple 2 Eas Ket Vila 4 i qe} ors Wo! > nae elim A Pasmiconee . i CAPA) te Jew fem, ~ Yratheenoopinae oy avatie® oa Prasmie © eqn Pee +. Prothepmipin Toi te On eld Mee ell i xi fed wrt 0 (ID Rbggegen $1 Fig 22 9 ibn ackweled in dw by untorct a of obs oF Mi of C2 Sepoce (ser) Sonmion petro : * Re imhales Tatviewic p., nin f @sysiee! * Wi wusd be madipiat by Alnnbin te beter Geral Va > Kelhierein = twihenkes M—*%a, fthin syste complet ~dierthy atively I —e Nea We (Cthamibit) pres: comers \— lo. PLASMIN - elat Aesalttion je fo Xe “oaivats Xi —* ila ves vy wu eee Conti Baten Reedoock) : i cf loom re ola Ot avabiahe (Tes fo) NGI achyathon, =r ‘ll Si by Reming He asl Riptide wn ae Sen ¢ i edhere, Sa TrameenesovLiy A Liminterts Ng NIN + Kill can eetiote Nil ovine Cc lorn an Corgi ey = Ctolloting Stone waitng fy Pe ovisnitt £18 a enn Wi Crates) ="Prediced by He LVER. except Il (ode my) Esterase ¢ activates \X elo Sviconnt TN 1 Coy VWF “egal Bilt ge1 Hs ong GX Aimer Rent’ PROPERTIES OF THE COAGULATION GROUPS Pies Prothrombin Fibrinogen 2a, Se WE LVI Xe N,V “Fapctea nares Protein G, Protein $. ¢;s ‘vit Serine proteases: Xi XI, PE Serine proteases: Vl, IK, Precursor of fb otactors tii mc Pretein C Cofactrs: = Cofactor: Protein S ‘Transglutaminase: Molecular weight ‘Medium: " E High, Stability Fairly stable Heat labile: VI, IX, X Heat labile: #v, 0 Well preserved in stored Storage labile: V, ae plasma ~Vitamin K dependent ea No. Yes no Cogepens “Adsorbabie Cb Ny re Fae te en! ‘Consumed in coagulation Partially No (except 1) Yes Site Plasma or serum Plasma or serum (except I Plast is not present in serum) “Destroyed by plasmin. oF No No ved high thrombin concentration ‘Found in platelets No No ‘Alpha-granule Cytoplas Not present. “tite phase reactants No No Yer Production reduced by No Yes No oral anticoagulants + - = Digest fn win ast YE. croluson oc Bi gepen a oes Sn ieeen mn cennyine Coenive prkoue) Cte by tre LIVER Udiseebes floan—# FOPs Vateed bain, heringen ,V 2 Vili yA nvoen) ¢ PLasinianetn) 59 ia, Falibecin Py ALD Coase) Rs At on poten “Yontionins om. meagan FIORIN * G> Woabn—r eli exksotor Forn Gechanis> rede On {ink nsic acai: Yl, Xia, Kalli HY, Proactvatee —|Abain is ae Onna advo, “TPA Coy oe celv ka (Kidney) ir fey 7 nea-cass > Metapevtic ack: SIEPTEKINEE swakinese TRA « Finn aeqtadanon ly Pron ~ _FPps a4 principal Hagments —~ Ki, EP Vedkeinese Lew eesr 8.14 | pn ol onan) Pasnin Degtodahan oF Orss-aped ra. oF th “No oxo Bos Pierce ain, 1 |) Coat pert a vey B/D Te yoy VI mi 4 h- Arkithremain Yh - sc a out ror 5 imbibe we D=DiMEr “is aaweaicator ap imviva Abnedysis| — Plasmn & Kalli * sodicans J of Fiona a fl CRsP) i tern = weak. jahibytee eX ely 1 ony deqraas ahon Products Is! C-+S cmb Net, a ¥DLE - low 4fQHON S-+o, "8 x Balla _cgact Es: eTa2-ankiplammin - Prieapol some . Y [Won Poteh hibiter xe x 7 Teh Whitten oF Hhewmbin YED -iombrr FeVIN polymenretierrPEC Couecmon a aise Prevent prematwe activation oF the clotting process Caused Fiat > 4. Costarmnation w/ T.twrumbeplostin - Sram DANGED TYE —9 TE ig rebrand f oxtvaies EP 2 lnapproprat cenbniner — Glass ~ achwates LP @ atvorts pls Gliely & 3. \onprger Temp - Lavie @RT: V & VI Preemomvaly sari @ REE tg: WAVEX\ a 4 oh weeny 4. Heananysis - Galicly Shotlened (ew ecwes Telibe goby — (Ef ESUPMENT —+ 20 9 - cong sa nr Chtaess 5 14. g - 720m ‘lead © Ant = Rein wide complees (rtf. in 00 ecting) —__ Comeong oF ohare) (a) t oe aa - rss f por al read saieng pw P.ggye Shialieg 5 ~ ideal SEL (aot) — Wp SON preted aoa te —+FRotonged\, EDTA - unsuitable y unttable Factor a Heparin - aus of anvthenebiy Ill —~ xi Al stays % eng Pr. velenthon tr) z & = changes can Prolong, cating hens 6 \D mins to = “Ips Cn Plone Cate Dae ot cr x ~ Use gal james, t Teer Se x mi ly ackvated € 4°C : WiLL x! [Rootme Walvation) EVALUATION A. \niosc J Comey pathway 4) Leet white WA cag inne = Neos Yaad in glass hee Solid clot = potas Neda 1.P 2 “ mgensitive te Ga Aeficiencies WN: 715 mins | PWR A1DXFSmm / (SX10O tubes ) 7 mevba © ae Foe Swing, Oneck every 40. seeomoly 2 = Moa CoN wing ela, ay cantains all ame ~ Abin cht PRE: eet soc Coser a) PRP: 150-240 secs em 8) APTr/ Py Haeee ore ~40 Re /plt Fecter % A Rodd: \ph + 25 9-49 kl = tala @ Pi. leels Ws 25 Rex Smtl = 0d wo — 100-HaK] al ~ Sighty J, 4100 [pl — AON We BT W0-taM|efa| — Low Bo -SO¥) nl ~ Porting -] Hourna 200 -4oK fal - LOOK [al ~ Sportomnedss Pxiding Yo\- SK, ~ Matty T LSk[pl -~ Seem se God- Wk fal - Moa. “T ? 7 . BOK] - Morfaaly T ». Pi. = Keg Ph A PRP = shape onange 4 cee am awe Lhe oe Io rin ax PRS - Roor wan't ope od = oly [Wetecenh9] 16 win odhetion Oafeel ¢ G-PR. odlhesivene cy x Geta aaah “satamann method” Woe 1 EDT) oe Gey [WER] u + TERMINOLOGIES - Yo. Wemagbysis. - Exprctworhon 1. Petdnias - pinterat Wemowhagic spots 8% blond! £? hy he narthoge 2. UTA - Vood i Small artas oF Skin, other deep hover. yp. Melena - block sha 3. Eechymosis . Wosd in Loiger overs X 10 deep tis i Merarthagia + Cress 4. Epistaxts - joscblera ren Bleeding, 5 Hemarthrsi's - blocot in joint avily G. Hema temenis ~ Seung #f blood 9. Hematoena - swelling / tmer mn Ksaves (uf blows) P-fematnia -intoct RECs im Unie @. Hemaghbinuna - ¥ oF He in Une Bleeding Alo lor ae gecks . 1. Hemel: CT dey 7 Eilers -Donlos /o - Inyperestennibie jomb j hyperplastic Skin, 7 Psevdoxanthema - Avy Shack a dhae bea Bactoum = G8 svoarocnneie bleralit L Wed erohar 7 Sn fey C (hot callogen: Seimtn of 8M ) 2 Sem > oging = degenciahen oF cOllagen, otc $-Hendony Nerorhans et wg 7“ ic 7H. Hemernonic \elanqiectana - yasoder malgiatn & sutace skin Lesions 7 Corgemtal Vemangiomate = a5 Aveo oF i we \eesds “Cswet & bled) on Kasaboan Ment s/> Swface A\vtorks Siqniant : 30°00 wa/tthe DM - Mionalounemin Ceatly onel of d-nethnpnty or 90 800 wa/mn (ALR) 7 Aronlovd dais - poster & Gnler by “amyloid”(~~ 8. tol Heroch’s = Absa pain 24 b8 G lending 7 AvRo immune Votahor Pwo = Allergic < coranlein = JOInt pam (Kees eres) we aos ngeine oe C- Quanh. pH. d/o 4. © Y ped'n — A.A : Gen. 8 Cppression Selective supp. oF Meg \oy Cneoliniatay TAR. X/L CARN BM meg, cimgentinl cheformila oF ater Waytlepthnse pracees: o epore Orauppng Iain Wm Bia *Dilutional ‘loss — extenve Blad Henlpaston + threnbiarhpenin + Non-temeune clasiwichon Kgicial suvgaces - prosthetic valves, the (pH micnoggregater) 7 \enaune PR: cachoson ee adic -b prt =a pomnajen st FOR: Dedimet, Li. cont 2 Hus, TTP. ptt. Seevertvodven, S naenbaile peg Brood Sprotwn oF Act fohtonic illaed 1 PH. d/o 1. Adine AE, 7 bs lock oF Geb yabX BT, Giant ph. «fo 2 WWD - leek op WE 7 Gatamewn’s T.- eg. of Gp2bfia 5 7 Afiennogenemin = © abs of Abomgen treet ap) -2 MA s]o - lack of Pqiendes 4d qtamler x monpite iHiwaby 7 7 Groy pit «/o - piah aes Conant) = 6 qranle che. : \s 7 Wermoneky - Petiak s/o — lack of fb (Aense) qranvier ~4nad of tynsinae C4) 7 Chediak -Hhaodhi «fe = fb Catense) otet = oher. by albiaum, wanent ings, € giont Hosemes(‘iBe,)) 4. 2 Sues = asprin, Cartanialh'y Combis pil. aggre) TASES = Myeloprl_ d/o, vitmia, Dic, Paroctinemias Dio_CE Vhronbosis AAe Aniitheembin des -peineipel intierte op teomion is denicient. Aimee de veer a8 Wiig 3) Rioainolytic Syston dfo oe < Tad V teden Catou cet be) oth Plosminogen) chep ap XN, X1, Hime, Kohl L Crean Peeing4. . ia) Fesistend fe Fosndysis S Ao plt-vessel wail interaction ° * Oniicomg - Procoag (mwa): jriniots ProsiacyQuN wes 3 Gctie ore ~ Assn] Twrantoacis dp: t prstooctin iahtion 4. Hemastarhc Cia! ABW 2 Fostop staies - vel. of Eee to > Malignancy - wrens 2 Pregnercy ~ Hocetin , EE Extuosc t c ‘eMMON a, Factor 9 deg Hhemephilie ©) | & Fach Vi) ole - ABN PT, © APT LTT ced tees ‘pkraing | b. Facer X der fo aT FP ¢- Fact V oe Onrer's dco b. Fothw wu: ¢ aey \ A. Foche N ley . Sey ca C. Fouts | bey Tx: Oyoppt / Facer vit aT Rabaneqenenie - © observe cc. oct Xa Comes Mews typo" doo eg. Cy lewis) A ne > Oy. 7 ABN /Acygan| x Foctw x ND fh one f chet Asha an Bu: yea, + Degets : Face VNC 1 WE AC coag 0 Abyinslysi Wepatic dee = cong facts : mote of PT Pb Lis FN ow. oe; xy eens Neowin end 1 blend * DIC = lypoccagulable tints 1° biog ysis- T oct. . pean gbsougen & bnfen un CGotrlegic pone gendycie) 22°F Weis =f cont. og ginoagtn ( Dic) 7 af aul LAR evalumhon oF Flmnnolysis a) WB clot ycis time Simson mY v \ hn harbor, — eel goeont'n. (TP = or I°Ciwaller) WPS ; Patacragy| = ageh feral CT prbsing oct - ABM (4 ay eee thon phone Wal: (AY GEL Rott Soh woe Speen ine int hy 9 - he dase 2 Feerualynp ~@) ony _€ 2° = GY agler Abs oF DIC onset —HEeand atari sxqey | Wersong ____| = Enhances nethnmbin fl, wytybits themes vit de A's Ge MEAS en yen iret = ky Madu famtin Ce = Prtomine sipate - Aer (mons