TOSOH BIOSCIENCE

ANALYSIS PROCESS INSTRUMENTATION

FURTHER INFORMATION

PRINCIPLES OF CHROMATOGRAPHY

To get an overview about the whole range

of TSKgel columns and small TOYOPEARL
and TSKgel bulk media, please request our
For a complete overview of all TOYOPEARL
and TSKgel bulk media, refer to our
Chromatographic Process Media Catalog.
ION EXCHANGE COLUMNS The analysis, isolation, and purifi­cation of biomolecules can be
accomplished by a number of chromatographic modes. Each
Chromatography Catalog.
mode is based on specific physical, chemical, or biological inter-
actions between the sample biomolecule and packing material.

Tosoh Bioscience offers a comprehensive line of TSKgel and

TOYOPEARL media and pre-packed TSKgel columns for all com-
mon modes of liquid chromatography.

The various modes of chromato­graphy involve separations that

are based on specific features of the target or sample, like size,
charge, hydropho­­bi­city, function or specific content of the mol-
ecule. To find out more about general principles of liquid chro-
matography and on how each of them works, visit us on our
YouTube channel.

For a deeper insight into applications and questions related to the practical use of TSKgel and
TOYOPEARL, check out the website www.tosohbioscience.de

Our technical experts are happy to discuss your specific separation needs by phone: +49 (0)6155-70437-36
or mail: techsupport.tbg@tosoh.com

TOSOH BIOSCIENCE

Im Leuschnerpark 4 64347 Griesheim, Germany

Tel: +49 6155-7043700 Fax: +49 6155-8357900 ION EXCHANGE CHROMATOGRAPHY
info.tbg@tosoh.com www.tosohbioscience.de
B16L01A
 TOSOH BIOSCIENCE ANALYSIS PROCESS INSTRUMENTATION

FURTHER INFORMATION

PRINCIPLES OF CHROMATOGRAPHY

To get an overview about the whole range

of TSKgel columns and small TOYOPEARL
and TSKgel bulk media, please request our
For a complete overview of all TOYOPEARL
and TSKgel bulk media, refer to our
Chromatographic Process Media Catalog.
ION EXCHANGE COLUMNS The analysis, isolation, and purifi­cation of biomolecules can be
accomplished by a number of chromatographic modes. Each
Chromatography Catalog.
mode is based on specific physical, chemical, or biological inter-
actions between the sample biomolecule and packing material.

Tosoh Bioscience offers a comprehensive line of TSKgel and

TOYOPEARL media and pre-packed TSKgel columns for all com-
mon modes of liquid chromatography.

The various modes of chromato­graphy involve separations that

are based on specific features of the target or sample, like size,
charge, hydropho­­bi­city, function or specific content of the mol-
ecule. To find out more about general principles of liquid chro-
matography and on how each of them works, visit us on our
YouTube channel.

For a deeper insight into applications and questions related to the practical use of TSKgel and
TOYOPEARL, check out the website www.tosohbioscience.de

Our technical experts are happy to discuss your specific separation needs by phone: +49 (0)6155-70437-36
or mail: techsupport.tbg@tosoh.com

TOSOH BIOSCIENCE

Im Leuschnerpark 4 64347 Griesheim, Germany

Tel: +49 6155-7043700 Fax: +49 6155-8357900 ION EXCHANGE CHROMATOGRAPHY
info.tbg@tosoh.com www.tosohbioscience.de
B16L01A
 TOSOH BIOSCIENCE ANALYSIS WWW.TOSOHBIOSCIENCE.DE

1

TOSOH BIOSCIENCE GMBH


2
TOSOH BIOSCIENCE LLC
3
TOSOH CORPORATION
TSKgel ANION EXCHANGE COLUMNS TSKgel CATION EXCHANGE COLUMNS
IM LEUSCHNERPARK 4
64347 GRIESHEIM


3604 HORIZON DRIVE,
SUITE 100
3-8-2 SHIBA, MINATO-KU
TOKYO 105-8623
ORDERING INFORMATION ORDERING INFORMATION
GERMANY KING OF PRUSSIA, PA 19406, USA JAPAN

ABOUT US
T + 49 (0) 6155 70437 00 T +1 484 805 1219 T +81 3 5427 5118
F + 49 (0) 6155 83579 00 F +1 610 272 3028 F +81 3 5427 5198 ORDERING INFORMATION ORDERING INFORMATION
INFO.TBG@TOSOH.COM INFO.TBL@TOSOH.COM INFO@TOSOH.CO.JP
WWW.TOSOHBIOSCIENCE.DE WWW.SEPARATIONS.US.TOSOHBIOSCIENCE.COM WWW.TOSOHBIOSCIENCE.COM Part # Description ID Length Particle Number Flow rate (mL/min) Maximum Part # Description ID Length Particle Number Flow rate (mL/min) Maximum
(mm) (cm) size (µm) theoretical Range pressure (mm) (cm) size (µm) theoretical Range pressure
plates drop (MPa) plates drop (MPa)
TSKgel GLASS COLUMNS: POLYMER-BASED TSKgel GLASS COLUMNS: POLYMER-BASED
WITH A GLOBAL PERSPECTIVE. 0013061 DEAE-5PW Glass, 100 nm 5.0 5.0 10 ≥ 700 0.5 - 0.8 1.5 0014012 CM-5PW Glass, 100 nm 20.0 15.0 13 ≥ 2,500 4.0 - 6.0 1.5
0008802 DEAE-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 1.0
Tosoh Bioscience is a leading manufacturer in the field of liquid 0014016 DEAE-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5
0013062
0008803
SP-5PW Glass, 100 nm
SP-5PW Glass, 100 nm
5.0
8.0
5.0
7.5
10
10
≥ 700
≥ 1,300
0.5
0.5
-
-
0.8
1.0
1.5
1.0
chromatography. The portfolio of over 500 specialty products 0018386 SuperQ-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0014017 SP-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5

encompasses instruments for size exclusion/gel permeation TSKgel PEEK COLUMNS: POLYMER-BASED
0019685 BioAssist Q, 400 nm 4.6 5.0 10 ≥ 500 0.3 - 1.0 2.5
TSKgel PEEK COLUMNS: POLYMER-BASED

chromatography and a comprehensive line of media and prepacked 1

0021410 BioAssist Q, 400 nm 10.0 10.0 13 ≥ 500 1.0 - 5.0 2.5 0019686
0021411
BioAssist S, 130 nm
BioAssist S, 130 nm
4.6
10.0
5.0
10.0
7
13
≥ 1,500
≥ 3,000
0.3 - 0.8
1.0 - 5.0
2.5
2.5
(U)HPLC columns for all common modes of liquid chromatogra- 2 3
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
0021960 Q-STAT, nonporous 3.0 3.5 10 > 200 1.0 - 2.0 10.0
phy. Over the last 40 years, TSKgel SW columns have become the 4
0021961 Q-STAT, nonporous 4.6 10.0 7 > 4,000 0.5 - 1.4 10.0 0021965 CM-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0021962 DNA-STAT, nonporous 4.6 10.0 5 > 4,000 0.3 - 0.6 15.0 0021966 CM-STAT, nonporous 4.6 10.0 7 ≥ 2,000 0.5 - 1.0 10.0
worldwide industry standard for size exclusion chromatography of 0013075 DEAE-NPR, nonporous 4.6 3.5 2.5 ≥ 1,300 1.0 - 1.5 20.0
0021963 SP-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0018249 DNA-NPR, nonporous 4.6 7.5 2.5 ≥ 6,000 0.5 - 1.0 30.0
biomolecules. 5 0018757 DEAE-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.5 0021964 SP-STAT, nonporous 4.6 10.0 7 ≥ 200 0.5 - 1.4 10.0
0007164 DEAE-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5 0013068 CM-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007574 DEAE-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5 0018758 SP-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.0
Tosoh manufacturing sites in Japan provide products to the sales 0007930
0018257
DEAE-5PW, 100 nm
SuperQ-5PW, 100 nm
55.0
7.5
20.0
7.5
20
10
≥ 1,500
≥ 1,300
20.0
0.5
-
-
40.0
1.0
0.4
2.0
0007161 SP-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007575 SP-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5
and support subsidiaries in the U.S. and Europe, ensuring full global 0018387 SuperQ-5PW, 100 nm
0008639 Sugar AXI, 6 nm
21.5
4.6
15.0
15.0
13
8
≥ 3,000
≥ 3,700
4.0
0.2
-
-
6.0
0.4
2.0
3.0 0007934 SP-5PW, 100 nm 55.0 20.0 20 ≥ 1,500 20.0 - 40.0. 0.4
coverage. Our technical specialists in the European Headquarters 0008640 Sugar AXG, 6 nm 4.6 15.0 10 ≥ 2,700 0.2 - 0.5 2.0 0013076
0007156
SP-NPR, nonporous
SCX (Na+), 6 nm
4.6
6.0
3.5
15.0
2.5
5
≥ 1,300
≥ 2,000
1.0 - 1.5
0.5 - 1.0
20.0
15.0
0007157 SAX, 6 nm 6.0 15.0 5 ≥ 2,000 0.5 - 1.0 15.0
provide assistance in developing HPLC applications or purification 4
TOSOH BIOSCIENCE SHANGHAI CO. LTD.
5
TOSOH ASIA PTE. LTD.
0007158 SCX (H+) 6 nm 7.8 30.0 5 ≥ 12,000 0.5 - 1.0 5.0
TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
methods, in up-scaling, or packing process columns. We offer chro- ROOM 101, INNOV TOWER, 63 MARKET STREET #10-03
BLOCK A, NO 1801 HONGMEI ROAD BANK OF SINGAPORE CENTRE 0018761 DEAE-2SW, 12.5 nm ­ 2.0 25.0 5 ≥ 5,000 0.12 - 0.17 13.0 TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
XU HUI DISTRICT SINGAPORE 048942, SINGAPORE 0007168 DEAE-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0 0007165 SP-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
matographic workshops, on-site training, and are the sole sponsor SHANGHAI, 200233, CHINA
T +86 21 3461 0856 T +65 6226 5106
0007163 DEAE-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0007167 CM-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
0007162 CM-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0
of the HIC/RPC Bioseparation Conference series. F +86 21 3461 0858 F +65 6226 5215
INFO@TOSOH.COM.CN INFO.TSAS@TOSOH.COM TSKgel GUARD COLUMN PRODUCTS
WWW.SEPARATIONS.ASIA.TOSOHBIOSCIENCE.COM WWW.TOSOHASIA.COM
0017088 DEAE-NPR Guard column 4.6 0.5 2.5 For P/N 0013075 GUARD COLUMN PRODUCTS
0018253 DNA-NPR Guard column 4.6 0.5 2.5 For P/N 0018249
TOSOH HISTORY 0018388 SuperQ-5PW Guardgel Kit 20 For P/N 0018257
0013069 CM-5PW Guardgel Kit 10 For P/N 0013068
1935 FOUNDING OF TOYO SODA MANUFACTURING CO., LTD. 0007211 SP-5PW Guardgel Kit 20 For P/N 0007161
0007210 DEAE-5PW Guardgel Kit 20 For P/N 0007164
1936 OPERATION OF NANYO MANUFACTURING COMPLEX BEGINS 0008807 SP-5PW Guardgel Kit, Glass 20 For P/Ns 0013062 and 0008803
0008806 DEAE-5PW Guardgel Kit, Glass 20 For P/Ns 0013061 and 0008802
0016093 SP-5PW Prep Guardgel Kit 20 For P/N 0007575
1971 SCIENTIFIC INSTRUMENTS DIVISION FORMED, FIRST GPC COLUMN USING TSKgel DEVELOPED BY TOSOH 0014466 DEAE-5PW Guardcol., Glass 20.0 2.0 13 For P/N 0014016
0007932 SP-5PW Guard column 45.0 5.0 20 For P/N 0007934
1974 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN PLANT IS COMPLETED 0016092 DEAE-5PW Prep Guardgel Kit 20 For P/N 0007574
0007650 CM-SW Guardgel Kit 20 For P/Ns 0007167 and 0007162
1979 TOSOH DEVELOPS TOYOPEARL MEDIA 0007928 DEAE-5PW Guard column 45.0 5.0 20 For P/N 0007930
1983 TOSOH DEVELOPS HYDROPHOBIC INTERACTION MEDIA 0007648 DEAE-SW Guardgel Kit 10 For P/Ns 0007168 and 0007163
1987 TOSOHAAS US OPERATIONS FORMED IN MONTGOMERYVILLE 0019308 Guard cartridge holder 2.0 1.5 For all 2 mm ID guard cartridges
1989 TOSOHAAS GMBH OPERATIONS FORMED IN STUTTGART

1995 TOSOH NANYO GEL FACILITY RECEIVES ISO 9001

2002/2003 ALL TOSOH AFFILIATED SCIENTIFIC & DIAGNOSTIC SYSTEM RELATED COMPANIES IN EUROPE ARE UNIFIED UNDER THE NAME TOSOH BIOSCIENCE.

2008 EcoSEC, THE 7TH GENERATION GPC SYSTEM IS INTRODUCED GLOBALLY

2010 TOSOH CELEBRATES ITS 75TH YEAR IN BUSINESS WITH THE OPENING OF FIVE NEW PLANTS, AND CONTINUED RAPID EXPANSION IN CHINA

2011 TOSOH BIOSCIENCE CELEBRATES 40 YEARS OF OPERATION

2012 TOSOH RELEASES FIRST TOYOPEARL MIXED-MODE RESIN TOYOPEARL MX-Trp-650M

2013 TOSOH RELEASES A HIGH CAPACITY PROTEIN A CHROMATOGRAPHY RESIN

2014 TOSOH BIOSCIENCE GMBH CELEBRATES ITS 25TH ANNIVERSARY IN STUTTGART

2015 TOSOH BIOSCIENCE SUCCESSFULLY MOVES ITS SALES & MARKETING OFFICES TO GRIESHEIM, DARMSTADT
 TOSOH BIOSCIENCE ANALYSIS WWW.TOSOHBIOSCIENCE.DE

1

TOSOH BIOSCIENCE GMBH


2
TOSOH BIOSCIENCE LLC
3
TOSOH CORPORATION
TSKgel ANION EXCHANGE COLUMNS TSKgel CATION EXCHANGE COLUMNS
IM LEUSCHNERPARK 4
64347 GRIESHEIM


3604 HORIZON DRIVE,
SUITE 100
3-8-2 SHIBA, MINATO-KU
TOKYO 105-8623
ORDERING INFORMATION ORDERING INFORMATION
GERMANY KING OF PRUSSIA, PA 19406, USA JAPAN

ABOUT US
T + 49 (0) 6155 70437 00 T +1 484 805 1219 T +81 3 5427 5118
F + 49 (0) 6155 83579 00 F +1 610 272 3028 F +81 3 5427 5198 ORDERING INFORMATION ORDERING INFORMATION
INFO.TBG@TOSOH.COM INFO.TBL@TOSOH.COM INFO@TOSOH.CO.JP
WWW.TOSOHBIOSCIENCE.DE WWW.SEPARATIONS.US.TOSOHBIOSCIENCE.COM WWW.TOSOHBIOSCIENCE.COM Part # Description ID Length Particle Number Flow rate (mL/min) Maximum Part # Description ID Length Particle Number Flow rate (mL/min) Maximum
(mm) (cm) size (µm) theoretical Range pressure (mm) (cm) size (µm) theoretical Range pressure
plates drop (MPa) plates drop (MPa)
TSKgel GLASS COLUMNS: POLYMER-BASED TSKgel GLASS COLUMNS: POLYMER-BASED
WITH A GLOBAL PERSPECTIVE. 0013061 DEAE-5PW Glass, 100 nm 5.0 5.0 10 ≥ 700 0.5 - 0.8 1.5 0014012 CM-5PW Glass, 100 nm 20.0 15.0 13 ≥ 2,500 4.0 - 6.0 1.5
0008802 DEAE-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 1.0
Tosoh Bioscience is a leading manufacturer in the field of liquid 0014016 DEAE-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5
0013062
0008803
SP-5PW Glass, 100 nm
SP-5PW Glass, 100 nm
5.0
8.0
5.0
7.5
10
10
≥ 700
≥ 1,300
0.5
0.5
-
-
0.8
1.0
1.5
1.0
chromatography. The portfolio of over 500 specialty products 0018386 SuperQ-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0014017 SP-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5

encompasses instruments for size exclusion/gel permeation TSKgel PEEK COLUMNS: POLYMER-BASED
0019685 BioAssist Q, 400 nm 4.6 5.0 10 ≥ 500 0.3 - 1.0 2.5
TSKgel PEEK COLUMNS: POLYMER-BASED

chromatography and a comprehensive line of media and prepacked 1

0021410 BioAssist Q, 400 nm 10.0 10.0 13 ≥ 500 1.0 - 5.0 2.5 0019686
0021411
BioAssist S, 130 nm
BioAssist S, 130 nm
4.6
10.0
5.0
10.0
7
13
≥ 1,500
≥ 3,000
0.3 - 0.8
1.0 - 5.0
2.5
2.5
(U)HPLC columns for all common modes of liquid chromatogra- 2 3
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
0021960 Q-STAT, nonporous 3.0 3.5 10 > 200 1.0 - 2.0 10.0
phy. Over the last 40 years, TSKgel SW columns have become the 4
0021961 Q-STAT, nonporous 4.6 10.0 7 > 4,000 0.5 - 1.4 10.0 0021965 CM-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0021962 DNA-STAT, nonporous 4.6 10.0 5 > 4,000 0.3 - 0.6 15.0 0021966 CM-STAT, nonporous 4.6 10.0 7 ≥ 2,000 0.5 - 1.0 10.0
worldwide industry standard for size exclusion chromatography of 0013075 DEAE-NPR, nonporous 4.6 3.5 2.5 ≥ 1,300 1.0 - 1.5 20.0
0021963 SP-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0018249 DNA-NPR, nonporous 4.6 7.5 2.5 ≥ 6,000 0.5 - 1.0 30.0
biomolecules. 5 0018757 DEAE-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.5 0021964 SP-STAT, nonporous 4.6 10.0 7 ≥ 200 0.5 - 1.4 10.0
0007164 DEAE-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5 0013068 CM-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007574 DEAE-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5 0018758 SP-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.0
Tosoh manufacturing sites in Japan provide products to the sales 0007930
0018257
DEAE-5PW, 100 nm
SuperQ-5PW, 100 nm
55.0
7.5
20.0
7.5
20
10
≥ 1,500
≥ 1,300
20.0
0.5
-
-
40.0
1.0
0.4
2.0
0007161 SP-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007575 SP-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5
and support subsidiaries in the U.S. and Europe, ensuring full global 0018387 SuperQ-5PW, 100 nm
0008639 Sugar AXI, 6 nm
21.5
4.6
15.0
15.0
13
8
≥ 3,000
≥ 3,700
4.0
0.2
-
-
6.0
0.4
2.0
3.0 0007934 SP-5PW, 100 nm 55.0 20.0 20 ≥ 1,500 20.0 - 40.0. 0.4
coverage. Our technical specialists in the European Headquarters 0008640 Sugar AXG, 6 nm 4.6 15.0 10 ≥ 2,700 0.2 - 0.5 2.0 0013076
0007156
SP-NPR, nonporous
SCX (Na+), 6 nm
4.6
6.0
3.5
15.0
2.5
5
≥ 1,300
≥ 2,000
1.0 - 1.5
0.5 - 1.0
20.0
15.0
0007157 SAX, 6 nm 6.0 15.0 5 ≥ 2,000 0.5 - 1.0 15.0
provide assistance in developing HPLC applications or purification 4
TOSOH BIOSCIENCE SHANGHAI CO. LTD.
5
TOSOH ASIA PTE. LTD.
0007158 SCX (H+) 6 nm 7.8 30.0 5 ≥ 12,000 0.5 - 1.0 5.0
TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
methods, in up-scaling, or packing process columns. We offer chro- ROOM 101, INNOV TOWER, 63 MARKET STREET #10-03
BLOCK A, NO 1801 HONGMEI ROAD BANK OF SINGAPORE CENTRE 0018761 DEAE-2SW, 12.5 nm ­ 2.0 25.0 5 ≥ 5,000 0.12 - 0.17 13.0 TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
XU HUI DISTRICT SINGAPORE 048942, SINGAPORE 0007168 DEAE-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0 0007165 SP-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
matographic workshops, on-site training, and are the sole sponsor SHANGHAI, 200233, CHINA
T +86 21 3461 0856 T +65 6226 5106
0007163 DEAE-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0007167 CM-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
0007162 CM-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0
of the HIC/RPC Bioseparation Conference series. F +86 21 3461 0858 F +65 6226 5215
INFO@TOSOH.COM.CN INFO.TSAS@TOSOH.COM TSKgel GUARD COLUMN PRODUCTS
WWW.SEPARATIONS.ASIA.TOSOHBIOSCIENCE.COM WWW.TOSOHASIA.COM
0017088 DEAE-NPR Guard column 4.6 0.5 2.5 For P/N 0013075 GUARD COLUMN PRODUCTS
0018253 DNA-NPR Guard column 4.6 0.5 2.5 For P/N 0018249
TOSOH HISTORY 0018388 SuperQ-5PW Guardgel Kit 20 For P/N 0018257
0013069 CM-5PW Guardgel Kit 10 For P/N 0013068
1935 FOUNDING OF TOYO SODA MANUFACTURING CO., LTD. 0007211 SP-5PW Guardgel Kit 20 For P/N 0007161
0007210 DEAE-5PW Guardgel Kit 20 For P/N 0007164
1936 OPERATION OF NANYO MANUFACTURING COMPLEX BEGINS 0008807 SP-5PW Guardgel Kit, Glass 20 For P/Ns 0013062 and 0008803
0008806 DEAE-5PW Guardgel Kit, Glass 20 For P/Ns 0013061 and 0008802
0016093 SP-5PW Prep Guardgel Kit 20 For P/N 0007575
1971 SCIENTIFIC INSTRUMENTS DIVISION FORMED, FIRST GPC COLUMN USING TSKgel DEVELOPED BY TOSOH 0014466 DEAE-5PW Guardcol., Glass 20.0 2.0 13 For P/N 0014016
0007932 SP-5PW Guard column 45.0 5.0 20 For P/N 0007934
1974 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN PLANT IS COMPLETED 0016092 DEAE-5PW Prep Guardgel Kit 20 For P/N 0007574
0007650 CM-SW Guardgel Kit 20 For P/Ns 0007167 and 0007162
1979 TOSOH DEVELOPS TOYOPEARL MEDIA 0007928 DEAE-5PW Guard column 45.0 5.0 20 For P/N 0007930
1983 TOSOH DEVELOPS HYDROPHOBIC INTERACTION MEDIA 0007648 DEAE-SW Guardgel Kit 10 For P/Ns 0007168 and 0007163
1987 TOSOHAAS US OPERATIONS FORMED IN MONTGOMERYVILLE 0019308 Guard cartridge holder 2.0 1.5 For all 2 mm ID guard cartridges
1989 TOSOHAAS GMBH OPERATIONS FORMED IN STUTTGART

1995 TOSOH NANYO GEL FACILITY RECEIVES ISO 9001

2002/2003 ALL TOSOH AFFILIATED SCIENTIFIC & DIAGNOSTIC SYSTEM RELATED COMPANIES IN EUROPE ARE UNIFIED UNDER THE NAME TOSOH BIOSCIENCE.

2008 EcoSEC, THE 7TH GENERATION GPC SYSTEM IS INTRODUCED GLOBALLY

2010 TOSOH CELEBRATES ITS 75TH YEAR IN BUSINESS WITH THE OPENING OF FIVE NEW PLANTS, AND CONTINUED RAPID EXPANSION IN CHINA

2011 TOSOH BIOSCIENCE CELEBRATES 40 YEARS OF OPERATION

2012 TOSOH RELEASES FIRST TOYOPEARL MIXED-MODE RESIN TOYOPEARL MX-Trp-650M

2013 TOSOH RELEASES A HIGH CAPACITY PROTEIN A CHROMATOGRAPHY RESIN

2014 TOSOH BIOSCIENCE GMBH CELEBRATES ITS 25TH ANNIVERSARY IN STUTTGART

2015 TOSOH BIOSCIENCE SUCCESSFULLY MOVES ITS SALES & MARKETING OFFICES TO GRIESHEIM, DARMSTADT
 TOSOH BIOSCIENCE ANALYSIS 1

IEC
ION EXCHANGE CHROMATOGRAPHY

IEC
Ion exchange chromatography (IEC) is one of the most TSKgel HPLC columns are packed with silica or polymer
frequently used chromatographic modes in the separation based porous or nonporous beads. They are well suited
and purification of biomolecules. It is a non-denaturing for a broad range of applications in R&D, quality control or
technique that is used for analysis and at all stages and reaction monitoring.
scales of purification: from micro scale purification to in-
dustrial scale downstream processing.

Tosoh Bioscience offers analytical and semi preparative For TSKgel bulk and TOYOPEARL bulk please refer to our
ion exchange HPLC columns as well as ion exchange resins Chromatographic Process Media Catalogue.
for large scale biopurification.
2 WWW.TOSOHBIOSCIENCE.DE

IEC
HOW IT WORKS
IEC

IEC retains molecules based on ionic interactions. The on the pH and ionic strength of the mobile phase. Accord-
stationary phase surface displays ionic functional groups ing to differences in their overall charge and surface charge
that interact with analyte ions of opposite charge. IEC is fur- distribution, proteins can be separated by IEC.
ther subdivided into cation exchange and anion exchange
chromatography. IEC takes advantage of the fact that the relationship be-
tween net surface charge and pH is unique for a specific
Anion exchange media carry positively charged groups that protein. At a pH, equivalent to its isoelectric point, a protein
attract negatively charged anions. Cation exchange resins has no net charge and will not interact with the charged
display negatively charged groups which attract positively stationary phase. At a pH above the pI the protein will have
charged cations. Charged target molecules are retained on a negative net charge and will therefore bind to a positively
the stationary phase but can be eluted by increasing the charged anion exchanger. At a pH below its pI it will have
concentration of a similarly charged ion that will displace a positive net charge and will consequently interact with a
the analyte or target ions from the stationary phase. negatively charged cation exchanger. By adjusting the pH
or the salt concentration of the mobile phase, separation
Proteins have numerous functional groups that can have can be optimized. For loading, the pH and ionic strength
both positive and negative charges. IEC separates proteins are selected in a way that the targets or analytes bind to the
according to their net surface charge, which is dependent stationary phase (Figure 1).

FIGURE 1

Acidic pH: all three Low acidic pH: red Low alkaline pH: Blue Very alkaline pH: all
components are and green are and green are three components
positively charged. positively charged negatively charged are negatively
They bind to cation and bind to cation and flow through charged and flow
exchangers and exchangers. Blue cation exchangers. through cation
elute by applying is negatively charged Red is still positively exchangers.
a salt gradient. and flows through. charged and binds.

Cation

Surface net
charge 0 pH

Anion

Acidic pH: all three Low acidic pH: red Low alkaline pH: red Alkaline pH: all
components are and green are is still positively three components
positively charged positively charged charged and flows are negatively
and flow through and flow through. through, blue and charged. They
anion exchangers. Blue is negatively green are negatively bind to anion
charged and binds charged and bind exchangers and
to anion exchangers. to anion exchangers. elute by applying
a salt gradient.
 TOSOH BIOSCIENCE ANALYSIS 3

IEC
INTRODUCTION TO TSKgel IEC COLUMNS

IEC
Elution is usually performed by changing the ionic strength ‘Strong’, respectively ‘weak’ refers to the extent that the ion
of the mobile phase by applying a salt gradient. As the exchange capacity varies with change in pH. Strong ion
salt concentration of the mobile phase increases, the salt exchange groups have a steep titration curve. They show
ions compete with the bound molecules for the functional no variation of their ionization state with the pH and remain
groups of the stationary phase. The higher the net charge of fully charged over a broad pH range.
the molecule, the higher the salt concentration needed for
elution. Very tightly bound compounds are removed at the Typical strong anion exchange groups are quaternary amine
end of the elution by a wash step with very high salt buffer. groups (Q & QAE type), typical strong cation exchange
groups are sulfo or sulfopropyl groups (S & SP type).
Ion exchange resins are classified as weak or strong ion ex- Carboxymethyl (CM, cationic) and diethylaminoethyl
changers. The terms strong and weak do not refer to the (DEAE, anionic) are weak ion exchange groups. Figure 2
performance of the resins or to the strength of interaction shows the pKa values for these ligands.
between resin and target.

FIGURE 2

PKa VALUES FOR ION EXCHANGE GROUPS

pKa values for Ion Exchange groups

Q, SuperQ, QAE pKa = 12.2

DEAE pKa = 11.5

Anion
7.0
De
Cation cr
ea
CM pKa = 4.7*
sin
g
re
sin S, SP pKa = 1.2
pK
a

*pKa Toyopearl GigaCap CM-650M = 3.6

The binding buffer pH is selected between the pI of the protein and

the pKa of the stationary phase.

TABLE 1

CATION EXCHANGE GROUPS STRUCTURE

Carboxymethyl (CM) Weak -O-CH2COO -

Sulfopropyl (SP) Strong -O-R-O-CH2-CH2-CH2-SO3 -

ANION EXCHANGE GROUPS

Diethylaminoethyl (DEAE) Weak -O-CH2-CH2-N+-(C2H5)2

Quarternary Ammonium (Q) Strong -O-R-N+-(CH3)3

Quarternary Aminoethyl (QAE) Strong -O-CH2-CH2-N+H-(CH3)3

4 WWW.TOSOHBIOSCIENCE.DE

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INTRODUCTION TO TSKgel IEC COLUMNS
IEC

Tosoh Bioscience offers a broad line of high efficiency col- TSKgel BioAssist columns are also based on methacrylate
umns for analysis and isolation of biomolecules by anion particles with larger pores (400 nanometer for BioAssist Q
and cation exchange chromatography. In either mode of and 130 nanometer for BioAssist S) providing better access
IEC the product line contains methacrylate and silica based to the functional groups for large proteins.
columns. Most of the available chemistries are offered in
analytical as well as semi-preparative formats. Particle The methacrylate chemistry also forms the backbone of
sizes range from 2.5 µm for fast analysis to 20 µm for pre- non-porous resin columns such as the TSKgel NPR and
parative purposes. Proteins, peptides, oligonucleotides and STAT series. Since rate-limiting pore diffusion is eliminated
nucleic acids are typical samples that are analyzed or iso- with nonporous particles, analysis time is often reduced by
lated on TSKgel IEC columns. as much as 80% without loss in resolution. Also, recoveries
are routinely greater than 90%.
PACKING MATERIALS AND CHEMISTRIES
TSKgel STAT columns are the latest addition to the IEC col-
Methacrylate, silica and polysterene are used as matrices umn line. An innovative bonding chemistry results in col-
for the TSKgel line of ion exchange columns. The base resins umns that show a reasonable sample capacity while tra-
are derivatized either with diethylaminoethyl (DEAE), qua- ditional non-porous resins usually show limited capacity
ternary ammonium (Q), sulfopropyl (SP) or carboxymehtyl due to lower surface area. Specific application needs are
(CM) functionalities to provide weak anion, strong anion, addressed by offering various column formats and particle
strong cation and weak cation exchangers, respectively. sizes.

Columns from the silica-based TSKgel SW series are typi- For special applications polystyrene based columns are of-
cally used in the separation of low molecular weight com fered as well. They are most suitable for analyzing small
pounds such as pharmaceuticals, nucleotides or small pep- molecular weight sugars, amino acids, individual nucleic
tides. acids and small drug candidates. For detailed information
on these special columns please refer to the chromatogra-
The methacrylate backbone chemistry provides a robust, phy catalog.
hydrophilic particle that is suitable as a support for high
performance analytical and preparative separations of bio-
molecules. The G5000 PW base resin of the TSKgel 5PW
series is a spherical particle with a mean pore size of 100
nanometer (1.000 Angström).

FEATURES BENEFITS
BioAssist Columns
High capacity even for larger proteins (1 million Da) Fewer runs to collect required sample amounts
Unique pore structure provides fast mass transfer Sharper peaks improve analysis and isolation
Biocompatible PEEK column hardware Less sample loss due to adsorption
Available in analytical and semi-prep formats Easy scale-up

Polymer-Based Ion Exchange Columns

Methacrylate backbone Mechanically and chemically stable (pH 2.0-12.0)
Withstands repeated cleaning with base, and use of
organic solvents, denaturants and surfactants
Large pore size (100 nm) (excl. limit for proteins ~ Use same column for most biopolymers
5,000,000 Da)
Non porous resin-based (STAT and NPR) columns Fast QC analysis and process monitoring
Several columns available in 2 mm ID format Reduced solvent consumption and analysis time

Silica-Based Ion Exchange Columns

Smaller pore size (2SW = 12.5 nm and 3SW = 25 nm) Most suitable for analysing smaller MW samples such
as nucleotides, drug candidates, catecholamines and
small peptides or proteins
 TOSOH BIOSCIENCE ANALYSIS 5

IEC
PROPERTIES OF TSKgel IEC COLUMNS

IEC
PROPERTIES OF TSKgel ION EXCHANGE COLUMNS

TSKgel ANION EXCHANGE COLUMNS

TSKgel Matrix*
Particle Pore Functional Counter Excl. limit, Capacity Small ion pKa Column
size (µm) size (nm) group ion PEG** (Da) (mg BSA/mL) capacity hard-
meq/mL ware***
BioAssist Q pMA 10, 13 ~400 Polyamine Cl- >5,000,000 70 0.1 9.4 PEEK
SuperQ-5PW pMA 10,13 100 Trimethyl-amino Cl- 1,000,000 100 > 0.13 12.2 S, G
DEAE-5PW pMA 10,13, 20 100 DEAE Cl- 1,000,000 30 0.1 11.5 S, G
Q-STAT pMA 7,10 ~ 0 Trimethyl-amino Cl- 500 20 0.27 10.5 S
DNA-STAT pMA 5 ~ 0 Trimethyl-amino Cl- 500 35 0.27 10.5 S
DEAE-NPR pMA 2.5 ~ 0 DEAE Cl- 500 5 > 0.1 11.2 S
DNA-NPR pMA 2.5 ~ 0 Proprietary ClO4- 500 5 > 0.1 11.2 S
DEAE-2SW Silica 5 12.5 DEAE H2PO4- 10,000 ND > 0.3 11.2 S
DEAE-3SW Silica 10 25 DEAE Cl- 30,000 ND > 0.3 11.2 S
Sugar AXI PS-DVB 8 6 Trimethyl-amino HBO3- ND > 1.2 12.5 S
Sugar AXG PS-DVB 10 6 Trimethyl-amino HBO - 3
ND > 1.2 12.5 S
SAX PS-DVB 5 6 Trimethyl-amino Cl- ND > 1.0 12.5 S

TSKgel CATION EXCHANGE COLUMNS

TSKgel
Particle Matrix*
Pore Functional Counter Excl. limit, Capacity Small ion pKa Column
size (µm) size (nm) group ion PEG** (Da) (mg/mL) capacity hard-
meq/mL ware***
BioAssist S pMA 7, 13 ~130 Sulfopropyl Na+ ~4,000,000 70(1) 0.1 2.4 PEEK
SP-5PW pMA 10, 13, 20 100 Sulfopropyl Na+ 1,000,000 40(2) > 0.1 2.3 S, G
CM-5PW pMA 10, 13 100 Carboxymethyl Na +
1,000,000 45 (2)
> 0.1 4.2 S, G
SP-STAT pMA 7, 10 ~ 0 Sulfopropyl Na
+
500 10 (3)
> 0.023 S
CM-STAT pMA 7, 10 ~ 0 Carboxymethyl Na
+
500 15 (3)
> 0.1 4.9 S
SP-NPR pMA 2.5 ~ 0 Sulfopropyl Na+ 500 5(2) > 0.1 2.3 S
SP-2SW Silica 5 12.5 Sulfopropyl Na +
10,000 ND 0.3 2.2 S
CM-2SW Silica 5 12.5 Carboxymethyl Na +
10,000 110(2) > 0.3 4.2 S
CM-3SW Silica 10 25 Carboxymethyl Na +
30,000 ND > 0.3 4.2 S
SCX PS-DVB 5 6 Sulfonic acid Na+, H+ ND > 1.5 S

*pMA = poly methacrylate; PS-DVB = polystyrene-divinylbenzene **Polyethylene glycol

***PEEK = polyethyletherketone, S = stainless steel, G = glass (1) γ-globulin; (2) hemoglobin; (3) lysozyme
6 WWW.TOSOHBIOSCIENCE.DE

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TSKgel IEC COLUMN SELECTION
IEC

SAMPLE TYPE MW RANGE (Da) TSKgel COLUMN pH RANGE

Amino Acids, Peptides and Proteins

Amino acids < 2,000 SAX 1.0 - 14.0
SCX 1.0 - 14.0
Peptides and small proteins < 10,000 Q-STAT 3.0 - 10.0
SP-STAT 3.0 - 10.0
CM-STAT 3.0 - 10.0
SCX 1.0 - 14.0
SP-2SW 2.0 - 7.5
CM-2SW 2.0 - 7.5
DEAE-2SW 2.0 - 7.5
Proteins > 10,000 up to ~ 5,000,000 BioAssist S 2.0 - 12.0
BioAssist Q 2.0 - 12.0
Q-STAT 3.0 - 10.0
SP-5PW 2.0 - 12.0
DEAE-5PW 2.0 - 12.0
CM-5PW 2.0 - 12.0
SP-STAT 3.0 - 10.0
CM-STAT 3.0 - 10.0
SP-NPR 2.0 - 12.0
DEAE-NPR 2.0 - 12.0
SuperQ-5PW 2.0 - 12.0

Nucleic Acids
Purines and pyrimidines DEAE-2SW 2.0 - 7.5
SP-2SW 2.0 - 7.5
Nucleosides SP-2SW 2.0 - 7.5
DEAE-2SW 2.0 - 7.5
Nucleotides Q-/DNA-STAT 3.0 - 10.0
DEAE-2SW 2.0 - 7.5
Oligonucleotides Q-/DNA-STAT 3.0 - 10.0
DEAE-5PW 2.0 - 12.0
DEAE-NPR 2.0 - 12.0
DNA-NPR 2.0 - 12.0
SuperQ-5PW 2.0 - 12.0
DNA, RNA, and PCR products Q-/DNA-STAT 3.0 - 10.0
DNA-NPR 2.0 - 12.0
DEAE-NPR 2.0 - 12.0
DEAE-5PW 2.0 - 12.0
DEAE-3SW 2.0 - 7.5

Other Molecules
Mono and disaccharides Sugar AXI, AXG 1.0 - 14.0
SCX 1.0 - 14.0
SAX 1.0 - 14.0
 TOSOH BIOSCIENCE ANALYSIS 7

IEC
TSKgel STAT SERIES

IEC
TSKgel STAT columns are designed for high efficiency PRODUCT HIGHLIGHTS TSKgel STAT SERIES
separation of biomolecules and low molecular weight com-
pounds. They provide superior performance at reduced Very efficient chromatography for high as well as low
analysis time. STAT columns are available in various for- MW solutes made possible by novel bonding chemistry
mats and sizes of the mono-disperse particles (5, 7 and and the absence of micro-pores
10 µm) to perfectly match specific application needs. High speed and high resolution analysis of
Biomolecules in HPLC and UHPLC
The surface of the hydrophilic non-porous particles con- Higher adsorption capacities and lower pressures
sists of an open access network of multi-layered ion ex- compared with smaller particle sized TSKgel NPR
change groups (carboxymethyl, sulfopropyl or quaternary columns
ammonium groups; see Figure 3). The innovative bonding 7 or 10 µm particles (TSKgel Q-STAT) and 5 µm
chemistry results in columns that show a reasonable sam- particles (TSKgel DNA-STAT)
ple capacity while traditional non-porous resins usually 7 or 10 µm particles for SP and CM chemistries
show limited capacity due to lower surface area.
APPLICATIONS WITH TSKgel STAT ANION EXCHANGE
For fast and ultrafast IEC analysis short columns (3 mm ID x COLUMNS
3.5 cm length) are packed with large 10 µm particles. They
are ideally suited for rapid candidate screening or process Nucleotides
monitoring. Longer columns (4.6 mm ID x 10 cm length)
packed with 7 µm particles were designed for high reso- Mono-, di-, and tri-nucleotides were separated with ex-
lution IEC separation. They are perfect for the analysis of cellent peak shape on a TSKgel DNA-STAT column. The
nucleic acids, mAb variants, protein aggregates or PEGylat- narrow, symmetrical peaks, as shown in Figure 4, demon-
ed proteins. The DNA-STAT columns (4.6 mm ID x 10 cm strate the absence of micropores on this new generation of
length) packed with 5 µm Q-type anion exchange resin are non-porous resin columns. TSKgel DNA-STAT columns are
optimized for the analysis of nucleic acids. also, as the name implies, first choice for large nucleic acid
fragments.
The relatively large particle sizes support fast separation at
moderate pressure while at the same time the proprietary
surface modification technology ensures a high density of
functional groups going along with high sample capacity.

FIGURE 3 FIGURE 4

SCHEMATIC DIAGRAM OF TSKgel STAT SERIES HIGH RESOLUTION SEPARATIONS OF NUCELOTIDES

C, A

Protein 95
2d-UDP
2d-ADP

85
Ionic group
2d-CMP

75
UV@260nm (1Abs/1000mV)

Hydrophilic
2d-CDP

ATP
TDP

2d-CTP

65
chain
2d-AMP

2d-UTP
2d-UMP
TMP

55
2d-GMP

2d-GDP

2d-GTP
TTP

45

35
U

25
T

15

5
0 5 10 15 20 25
Retention time (min)
Column: TSKgel DNA-STAT, 5 µm, 4.6 mm ID x 10.0 cm L;

Column:
Eluent: A: 20 mmol/L TSKgel DNA-STAT,
Tris-HCI (pH 8.5); B: 0.755µm,
mol/L4.6mm
NaCl inID x 10cm
buffer A;
Eluent: A: 20mmol/L
Gradient: 50% B (0 min), Tris-HCl
75% B (25 min); Flow (pH8.5)
rate: 0.8 mL/min;
Non porous material Detection: UV @ 260 B:
nm0.75mol/L NaCl in buffer A
Gradient: 50% B (0min), 75% B (25min)
Flowrate: 0.8mL/min
Detection: UV@260nm
8 WWW.TOSOHBIOSCIENCE.DE

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TSKgel STAT SERIES
IEC

FIGURE 5
APPLICATIONS WITH TSKgel STAT ANION EXCHANGE
COLUMNS ANALYSIS OF IgG FRAGMENTS

Monoclonal Antibodies

The monoclonal antibody was digested using pepsin and

separated on a TSKgel Q-STAT column and a competi- (B)

mV
tive non-porous WAX column. As shown in Figure 5, three
peaks were isolated from the TSKgel Q-STAT column and
assigned as F(ab‘)2, pFc and intact IgG by SDS-PAGE. There
was no correlation between the peaks obtained on the com- (A)
petitive WAX column and SDS-PAGE.
0 2 4 6 8 10
Retention time (min)
High Resolution versus high Throughput Analysis of
Nucleotides

The separation of nucleotides on a 10 cm length Q-STAT F(ab’)2

column was compared to the separation on a 3.5 cm length
pFc
column. While the longer column provides a high resolution Intact IgG
separation of the nucleotides within five minutes the same
sample can be analyzed within one minute on the shorter 200kDa

column allowing high throughput. The chromatograms are

116kDa
shown in Figure 6.

14.4kDa
6.5kDa

Non-reduced SDS -PAGE

Columns: A: TSKgel Q-STAT, 7µm, 4.6mm ID x 10cm
Column: A: TSKgel Q-STAT, 7 µm,B: ProPac WAX-10,
4.6 mm ID x 10µm,
10 cm4mm IDCompetitor
L: B: x 25cm WAX,
Eluent: A: 20mmol/L Tris-HCl (pH8.5)
10 µm, 4 mm ID x 25 cm L; Eluent: A: 20 mmol/l Tris-HCI (pH 8.5); B: 0.5
B: 0.5mol/L NaCl in buffer A
mol/L NaCl in buffer A; Gradient:
Gradient: 0% B0% B (0 100%
(0min), min),B100%
(10min)B (10 min); Flow rate:
Flow-rate:UV @ 280
1.0 mL/min; Detection: 1.0mL/min
nm; Samples: pepsin digested mAb
Detection: UV@280nm
Sample: pepsin digested mAb
FIGURE 6
HIGH RESOLUTION VERSUS HIGH THROUGHPUT ANALYSIS OF NUCLEOTIDES

High resolution: High throughput:

Column: Q-STAT, 4.6 mm ID x 10 cm L (7 µm); Eluent: A) 20 mmol/L Column: Q-STAT, 4.6 mm ID x 3.5 cm L (10 µm); Eluent: A) 20 mmol/L
Tris-HCl (pH 8.5) B) 0.5 mol/L NaCl in A (pH 8.5); Gradient: 0 to 100% B Tris-HCl (pH 8.5), B) 0.5 mol/L NaCl in A (pH 8.5); Gradient: 0 to 100% B
(10 min.); Flow rate: 1.5 mL/min.; Detection: UV @ 260 nm (1 min.); Flow rate: 4.0 mL/min.; Detection: UV @ 260 nm
 TOSOH BIOSCIENCE ANALYSIS 9

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TSKgel STAT SERIES

IEC
APPLICATIONS WITH TSKgel STAT CATION EXCHANGE Reaction Monitoring
COLUMNS
A sample of β-lactoglobulin (5 mg/mL) was reacted with
Fast Separations polyethylene glycol (5 kDa) in a pH 6.5 phosphate buf-
fer. The formation of PEGylated protein reaction prod-
The fast separation of protein standards was investigated ucts was monitored in 5 minute intervals on a 3.5 cm L
using short cation exchange columns (see Figure 7). A TSKgel TSKgel SP-STAT column. As demonstrated in Figure 8,
SP-STAT column shows superior resolution, better peak peak areas of mono-, di-, and tri- PEGylated β-lactoglobulin
shape, and shorter analysis time (< 60 seconds) compared increased with reaction time, while the area of unreacted
to a competitive monolithic SP-type column. β-lactoglobulin declined.

FIGURE 7 FIGURE 8

FAST PROTEIN SEPARATION PEGYLATION MONITORING

50

45
1
2 40 Native
UV @ 280 nm (1 Abs/1000 mV)

35
3
30

Mono- PEG
25
(B)
mV

20 Di- PEG

Tri -PEG
15

10

5
0 0.5 1 1.5 2 2.5
(A) Retention time (min)
100

0 0.2 0.4 0.6 0.8 1 1.2 90

Native
Retention time (min)
80

Columns: A: TSKgel Q-STAT, 10µm, 3.0mm ID x 3.5cm 70

Column: A: TSKgel SP-STAT, 10 µm, 3.0 mm ID x 3.5 cm L;
B: ProSwift SCX-1S Monolith, 4.6mm ID x 5cm
B: Competitor column 4.6 mm ID x 5.0 cm L
Eluent:  A: 20mmol/L sodium acetate (pH5.0) 60
Eluent: A: 20 mmol/L sodium acetate (pH 5.0); B: 1.0 mol/L NaCl in buffer
B: 1.0mol/L NaCl in buffer A (pH5.0) for column A
Peak %

A (pH 5.0) for column A; 1.5 mol/l NaCl in buffer A (pH 5.0) for column B; 50
1.5mol/L NaCl in buffer A (5.0) for column B
Gradient: 0% B (0 min), 100% B (1 min);
Gradient: 0% B (0min), 100% B (1min)
Flow rate: A: 2.0 mL/min; B: 4.73 mL/min; Detection: UV @ 280 nm; 40
Flow rate: A: 2.0mL/min
Samples: 1. α-chymotrypsinogen A; 2. cytochrome C; 3. lysozyme Mono-PEG
B: 4.73mL/min 30
Detection: UV@280nm
Samples: 1. alpha-chymotrypsinogen A 20
2. cytochrome C Di -PEG
10
3. lysozyme
Tri- PEG
0
0 10 20 30 40 50 60 70
Reaction time (min)

Column:
Column: A: TSKgel
TSKgel SP-STAT, SP-STAT,
10 µm, 3.0 10µm,
mm ID x 3.5 3mm
cm L; ID xA:3.5cm
Eluent: 20 mmol/L
Eluent:
sodium A: B:
acetate (pH 5.0); 20mmol/L
1.0 mol/Lsodium acetate
NaCl in buffer buffer
A (pH 5.0);(pH5.0)
Gradient: 0% B (0 min),B: 1.0mol/L
100% NaClFlow
B (2 min); in buffer A (pH5.0)
rate: 2.0 mL/min;
Gradient: @ 280 nm;
Detection: UV 0%Samples:
B (0min),PEGylated
100% B (2min)
β-lactoglobulin
Flowrate: 2.0mL/min
Detection: UV@280nm
Sample: pegylated β-lactoglobulin
10 WWW.TOSOHBIOSCIENCE.DE

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TSKgel STAT SERIES
IEC

Antibody Analysis Column Selection for Antibody Analysis

The analysis profiles for five antibodies separated on a Different antibodies were analyzed on a CM-STAT and SP-
TSKgel CM-STAT column were compared with the profiles STAT column at pH 7. Figure 10 shows that it is dependent
obtained on a competitive WCX column (Figure 9). Similar on the antibody which column provides the better resolu-
or higher resolution profiles were obtained on TSKgel CM- tion. The strong cation exchange column TSKgel SP-STAT
STAT in approximately half the time. shows a better separation of charge variants of mAb A (up-
per chromatograms) while the weak cation exchange col-
umn TSKgel CM-STAT delivers a better separation of a ba-
sic variant from the main peak for mAb B.

FIGURE 9 FIGURE 10
ANALYSIS OF CHARGE HETEROGENEITY COMPARISON OF TSKgel CM- AND SP-STAT COLUMNS
40
A
30

E
mV

20
D
C
10
B
A
0

0 10 20 30 40
Retention time (min)

B
15

10
E
mV

D
5 C
B

A
0

0 20 40 60 80
Retention time (min)
Columns: A: TSKgel
Column: A: TSKgel CM-STAT, CM-STAT,
7 µm, 4.6 mm ID7µm, 4.6mm
x 10 cm L; B: ID x 10cm
Competitor WCX, 10 B: µm,ProPac
4.0 mm WCX-10,
ID x 25 cm 10µm,
L; 4mm ID x 25cm
Eluent: A: 20mmol/L
Eluent: A: 20 mmol/L MES (pH 6.0) ; B: MES (pH6.0)
20 mmol/L MES + 0.5 mol/L NaCl (pH 6.0) Columns: TSKgel SP-STAT (7 µm, 4.6 mm ID x 10 cm);
Gradient: A: 10% B (0 B:min),
20mmol/L
30% B MES + 0.5mol/L
(15 min), NaCl
100% B (15 (pH6.0)
min), 100% B (17 TSKgel CM-STAT (7 µm, 4.6 mm ID x 10 cm)
Gradient:
min), 10% B (17 min), A:
10%10%B (21Bmin);
(0min), 30%B (0
B: 10% B min),
(15min),
30%100% B (15min),
B (30 min), 100% Mobile phase A: 10 mmol/L sodium phosphate buffer pH 7.0
100%
B (30 min), 100% B (32 min), 10%B B(17min),
(32 min),10%
10%BB(17min),
(36 min) 10% B (21min) Mobile phase B: 100 mmol/L sodium phosphate pH 7.0 + 500 mmol/L NaCl
B: 10%
Flow rate: A: 1.0 mL/min B (0min),
B: 2.0 mL/min;30% B (30min),
Temp.: Ambient;100% B (30min),
Detection: UV @ Gradient: 0-100 % B in 30 min; Flow rate: 1 mL/min; Detection: UV @ 280 nm
100% Bmonoclonal
280 nm; Inj. Vol.: 20 µL; Sample: (32min), 10% B (32min),
antibodies (mAb10% B (36min)
A through E) Injection vol.: 10 µL; Sample: mAb A (2 g/L); mAb B (2 g/L)
Flowrate: A: 1.0mL/min
B: 2.0mL/min
Temp.: Ambient
Detection: UV@280nm
 TOSOH BIOSCIENCE ANALYSIS 11

IEC
TSKgel BioAssist SERIES

IEC
TSKgel BioAssist columns are based on methacrylate Especially designed for the separation of large biomole-
particle design technology. TSKgel BioAssist Q contains cules the very large pores of the TSKgel BioAssist columns
particles with very large pores (~400 nm) that are deriva- offer high capacity and resolution at a low column pres-
tized with a network of polyamine groups. The capacity of sure drop. The polymerization technique used to construct
TSKgel BioAssist Q has been shown to be high over a these columns results in a homogenous distribution of ion
wide molecular weight range (up to 1,000,000 Da). TSKgel exchange groups without significantly reducing pore size.
BioAssist S is packed with particles possessing 130 nm
pores functionalized with sulfopropyl groups. APPLICATIONS WITH TSKgel BioAsisst ANION
EXCHANGE COLUMNS
TSKgel BioAssist analytical IEC columns are provided in a
4.6 mm ID x 5 cm L PEEK housing with 7 μm or 10 μm par- Performance Enhancement on FPLC System
ticles for the respective S and Q functionalities.
TSKgel BioAssist Q is suitable for use in systems that are
Semipreparative TSKgel BioAssist columns are also avail- designed for laboratory or semi-preparative applications.
able with a 13 μm particle size packed in a 10 mm ID x 10 cm L Figure 11 demonstrates the performance enhancement of
housing. The longer length of the semi-preparative column TSKgel BioAssist Q over a competitive product when oper-
compensates for the increased particle size, resulting in ated side-by-side on an FPLC system.
similar resolution to the analytical column.

PRODUCT HIGHLIGHTS TSKgel BioAssist SERIES FIGURE 11

PERFORMANCE ENHANCEMENT ON FPLC SYSTEM
Performance enhancement on FPLC system
Pore structure and bonding chemistry of TSKgel mAU
1
BioAssist Q/S columns provide high capacity for 1200 2
BioAssist Q
small to very large MW proteins and nucleic acids. 1180
1600 3
TSKgel BioAssist Q/S is suitable for use in systems
1140
that are designed for HPLC, laboratory or semi-
1120
preparative applications i
1100
TSKgel BioAssist columns are packed in 4.6 mm ID 1080
or 10 mm ID PEEK hardware. Other columns are 1060
available in glass and stainless steel for analytical, 1040
semi-preparative and preparative applications.
mAU
2
1200 1 3

1180
1600 Competitor Q
1140
1120
i
1100
1080
1060
1040

0 5 10 15
Minutes
Column: TSKgel BioAssist Q, 4.6 mm ID x 5 cm (PEEK)
Column: TSKgel BioAssist Q, 4.6 mm ID x 5 cm L (PEEK),
Competitor Q, 5.0 mm ID x 5 cm
Competitor
Elution: Q, 5.0
30mm ID x 5gradient
min linear cm L; Elution:
from 0 to301 min
mol/Llinear
NaClgradient from
0 to 1 mol/L NaClinin20
20mmol/L
mmol/Lsodium
sodiumphosphate
phosphatepH pH8.0
8.0; Flow Rate: 1.0 mL/
Flow Detection:
min; Rate: 1.0 mL/min
UV@ 280 nm; Sample: 1) conalbumin, i) ovalbumin impurity,
Detection: UV @ 280
2) ovalbumin, 3) trypsin nm
inhibitor
Sample: 1) conalbumin, i) ovalbumin impurity
2) ovalbumin, 3) trypsin inhibitor
12 WWW.TOSOHBIOSCIENCE.DE

IEC
TSKgel BioAssist SERIES
IEC

APPLICATIONS WITH TSKgel BioAsisst ANION APPLICATIONS WITH TSKgel BioAssist CATION
EXCHANGE COLUMNS EXCHANGE COLUMNS

Comparison of Dynamic Binding Capacity Bromelain Anaylsis on TSKgel BioAssist S and Competitor
S Column
Table 2 shows typical dynamic binding capacities on Bio-
Assist Q relative to competitive products. Figure 12 shows the analysis of bromelain, a proteolytic
enzyme that is used as a nutritional supplement. Bromelain
TABLE II is a basic glycoprotein with a MW of 33 kDa and a pI of 9.55.
COMPARISON OF DYNAMIC BINDING CAPACITIES
Analysis of IgM
Binding capacity (mg/mL)
BioAssist Q SuperQ Conv. Conv. IgM is known to possess unique and beneficial charac-
-5PW Q type Q type teristics relative to other immunoglobulin classes; it is a
Protein prod. A prod. B large molecule comprised of five IgG subunits, resulting in
Thyroglobulin 77.4 22.9 20.2 1.8 a relatively unstable and difficult to purify protein. Unlike
Monoclonal IgG1 57.8 43.3 46.7 47.7 single chain antibodies, IgM cannot be purified by Protein A
Human Serum 83.1 78.9 48.2 48.8 (affinity material commonly used for its high binding capacity
Albumin and excellent selectivity for antibodies) due to steric hin-
Trypsin Inhibitor 84.3 92.8 51.8 57.8 drance. Alternative affinity methods have been developed
with thiophillic absorbents but these methods often result
Columns: TSKgel BioAssist Q and T
SKgel SuperQ-5PW (4.6 mm ID x 1 cm); in low binding capacity. An alternative purification method
Conventional Q type product A and B (4.6 mm ID x 1 cm) of IgM by ion exchange chromatography using a TSKgel
Solvent: 20 mmol/L Tris-HCl buffer, pH 8.0; BioAssist S column was developed. Figure 13 shows the
Flow rate: 0.38 mL/min; Det.: UV @ 280 nm baseline separation of IgM from other contaminants using
*Capacity was determined at 10% height of the breakthrough curve; UV 280 nm. a 0.3 mol/L NaCl step gradient after elution of albumin.

FIGURE 12 FIGURE 13

BROMELAIN ANALYSIS ON TSKgel BioAssist S & COMPETITOR S ANALYSIS OF IgM

COLUMNS
Bromelain Analysis on TSKgel Bioassist S and Competitor S Columns
3.0 0.8
500
1
0.7
TSKgel BioAs
2.5
400
Competitor S 0.6 Mobile phase
Gradient
2.0
0.5
NaCl (M) in eluent

300
Flow Rate:
Abs (280mm)

1.5 0.4 Detection:

Sample:
200 0.3
2
1.0
0.2
100
0.5
BioAssist S
0.1

0 0.0 0
0 5 10 15 20 25 0 5 10 15
Retention time
Retention time (minutes)
Columns: TSKgel BioAssist S, 4.6 mm ID x 5 cm L, PEEK Column: TSKgel BioAssist S, 7 µm, 4.6 mm ID x 5 cm L;
Competitor S 5 mm ID x 5 cm L; Elution: 20 min (TSKgel) or 30 min (Competitor Mobile phase: 20 mmol/L sodium phosphate buffer, pH 6.0;
S) linear gradient of NaCl from 0 to 0.5 mol/L in 20 mmol/L sodium phosphate Gradient: 0 mol/L - 0.3 mol/L NaCl (5 min), 0.3 mol/L - 0.5 mol/L NaCl (10 min);
buffer, pH 7.0; Flow rate: 0.8 mL/min for TSKgel; 1.0 mL/min for Competitor S Flow rate: 1 mL/min; Detection: UV @ 280 nm; Sample: 500 µL of 9.5 mg/mL IgM
Detection: UV @ 280 nm; Temperature: 25°C; in mouse ascites fluid; shaded peaks represent albumin and IgM respectively
Sample: crude bromelain (C4882, Sigma), 1 mg in 100 μL

Columns: TSKgel BioAssist S, 4.6mm ID x 5cm, PEEK

Competitor S 5mm ID x 5cm
Elution: 20 min (TSKgel) or 30 min (Competitor S) linear gradient of NaCl
from 0 to 0.5mol/L in 20mmol/L sodium phosphate buffer, pH 7.0
 TOSOH BIOSCIENCE ANALYSIS 13

IEC
TSKgel 5PW SERIES

IEC
The G5000 PW base resin of the TSKgel 5PW series is a Analysis of high MW RNA on TSKgel DEAE-5PW
spherical particle with a mean pore size of 100 nanometer.
The chemistries of DEAE, SP and CM functionalities result Figure 15 shows the fractionation of high molecular weight
in standard ion exchangers while the chemistry employed E. coli RNA on TSKgel DEAE-5PW, effectively utilizing the
in the manufacturing of TSKgel SuperQ-5PW results in a large 100 nm pores of this base resin.
higher capacity strong anion exchanger by introducing
polyamine functional groups. Due to the higher density of
anion exchange sites TSKgel SuperQ-5PW has a smaller
effective pore size and a higher binding capacity than
TSKgel DEAE-5PW.

APPLICATIONS WITH TSKgel 5PW ANION EXCHANGE

COLUMNS

Analysis of a synthetic Oligonucleotide on TSKgel SuperQ-

5PW

Figure 14 shows the analysis of a 16-mer morpholine oligo-

nucleotide on TSKgel SuperQ-5PW column using a NaCl
gradient in a 10 mmol/L sodium hydroxide mobile phase.

FIGURE 14 FIGURE 15

ANALYSIS OF SYNTHETIC OLIGONUCLEOTIDE ON TSKgel LARGE PORE TSKgel DEAE-5PW RESOLVES HIGH MW RNA
SuperQ-5PW
Analysis of synthetic oligonucleotide on TSKgel SuperQ-5PW Large pore TSKgel DEAE-5PW resolves high MW RNA
16S rRNA
High MW
Impurities

23S rRNA

4S tRNA
5S rRNA

0 10 20 30 40 0 40 80
Minutes Minutes
Column: Column: TSKgelTSKgel SuperQ-5PW,
7.5 mm ID 7.5mm
x 7.5 cmID
L; x 7.5cm
SuperQ-5PW, Column: TSKgel TSKgel
Column: DEAE-5PW,
DEAE-5PW, 6 mm ID x6mm IDL;x Sample:
15 cm 15cm total E. coli RNA;
Sample: Sample: 16-mer16-mer morpholine oligonucleotide,
morpholine oligonucleotide, AAG AAG AAG AGG GGA G; Sample:300 mintotal
Elution: linearE.gradient
coli RNAfrom 0.3 mol/L NaCl in 0.1 mol/L Tris-HCI,
AAG AAG AAG AGG GGA G
Sample load: 0.5 O.D. (optical density); Mobile phase: A: 10 mmol/L NaOH; Elution:
pH 300min
7.6; Flow rate:1.0 linear
mL/min; gradient
Detection: UVfrom 0.3mol/L
@ 260 nm to 1.0mol/L NaCl
Sample load: 0.5 O.D. (optical density)
B: 10 mmol/L NaOH with 1 mol/L NaCl; Gradient: Initial: 0 % B, 40 min: 50 % B, in 0.1mol/L Tris-HCl, pH 7.6
Mobile phase: A: 10mmol/L NaOH Flow Rate: 1.0mL/min
41 min: 100 % B, 46 min: 100% B; Flow rate: 1 mL/min; Detection: UV @ 254 nm
B: 10mmol/L NaOH with 1mol/L NaCl Detection: UV @ 260nm
Gradient: Initial: 0% B
40min: 50% B
41min: 100% B
46min: 100% B
Flow Rate: 1 mL/min
Detection: UV @ 254nm
 14 WWW.TOSOHBIOSCIENCE.DE

IEC
TSKgel 5PW SERIES
IEC

APPLICATIONS WITH TSKgel 5PW CATION EXCHANGE

COLUMNS

Comparison of strong and weak Cation Exchangers Semi-preparative Purification of Lipoxidase

Differences in selectivity between strong (TSKgel SP-5PW) The purification of 200 mg of crude lipoxidase on a
and weak (TSKgel CM-5PW) cation exchangers are demon- 21.5 mm ID TSKgel SP-5PW column is illustrated in
strated in Figure 16 which is a separation of globular pro- Figure 17. Scale-up is simplified as only the particle size
teins. changes from 10 μm (7.5 mm ID) to 13 μm (21.5 mm ID) or
20 μm (55 mm ID) colum.

FIGURE 16 FIGURE 17

SELECTIVITY ON TSKgel STRONG AND WEAK CATION SEMI-PREPARATIVE PURIFICATION OF LIPOXIDASE

Selectivity of TSK-GEL strong and weak cation exchangersSemi-preparative purification of lipoxidase
EXCHANGERS

SP-5PW
5

1 2 4 4

3 4

CM-5PW 5
1 2

4 0 30 60
Minutes

Column: TSKgel SP-5PW, 21.5 mm ID x 15 cm L; Sample: crude lipoxidase,

Column:
200 mg; Elution: TSKgel
120 min SP-5PW,
linear gradient fromID
21.5mm 0 mol/L
x 15cmto 0.5 mol/L Na2SO4 in
Sample: crude
0.02 mol/L acetate, lipoxidase,
pH 4.5; Flow rate:200mg
4.0 mL/min; Detection: UV @ 280 nm;

0 20 40
Elution: 120min
Recovery: Lipoxidase linearcollected
activity gradientbetween
from 0mol/L to vertical
the two 0.5mol/Llines SO4 in
Na2was
84%. 0.02mol/L acetate, pH 4.5
Minutes Flow Rate: 4.0mL/min
Columns: TSKgel SP-5PW and TSKgel CM-5PW, 7.5 mm ID x 7.5 cm L; Detection: UV @ 280nm
Columns: TSKgel SP-5PW and TSKgel CM-5PW, 7.5 mm ID x 7.5 cm L
Recovery: Lipoxidase activity collected between the two
Sample: 1. trypsinogen, 2. ribonuclease A, 3. a-chymotrypsinogen, 4. cyto-
Sample:chrome C,1.5.trypsinogen,
lysozyme;
2. ribonuclease A, 3. a-chymotrypsinogen, vertical lines was 84%
4. cytochrome C, 5. lysozyme
Elution: 60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl in 0.02 mol/L
Elution: phosphate,60pH
min
7.0;linear gradient
Flow rate: from
1.0 mL/min; 0 mol/L
Detection: UVto
@ 0.5 mol/L NaCl in
280 nm
0.02 mol/L phosphate, pH 7.0
Flow Rate: 1.0 mL/min
Detection: UV @ 280 nm
 TOSOH BIOSCIENCE ANALYSIS 15

IEC
TSKgel NPR SERIES

IEC
TSKgel DEAE-NPR, DNA-NPR and SP-NPR are packed with APPLICATIONS OF TSKgel NPR ION EXCHANGE
2.5 μm particles. High column efficiency coupled with low COLUMNS
sample capacity restricts the application of these columns
to fast analysis and micro-scale preparative isolation. TSKgel DEAE-NPR and DNA-NPR Anion Exchangers

The DNA-NPR column is a longer version of the DEAE-NPR Because of their small particle size, non-porous resin (NPR)
column that allows improved resolution of oligonucle- columns excel in rapid separations of large biomolecules
otides, including those amplified by PCR. Small guard col- such as DNA digests. A chromatogram of a standard Hae III
umns are available to protect the DNA-NPR and DEAE-NPR digest of pBR322 DNA on TSKgel DEAE-NPR, protected by
columns. a guard column, is shown in Figure 18.

To achieve better resolution for PCR fragment analysis we

recommend the use of TSKgel DNA-NPR columns, which are
7.5 cm long and 4.6 mm wide, providing higher efficiency
in a longer column.

TSKgel SP-NPR Cation Exchanger

TSKgel SP-NPR columns provide fast separations due to

their small spherical particles. A purity check of adeno-as-
sociated virus, commonly used in gene therapy research,
on a TSKgel SP-NPR column is shown in Figure 19. This 10
minute HPLC method replaces an existing assay that took
two days.

FIGURE 18 FIGURE 19

HIGHER RESOLUTION AND FASTER ANALYSIS ON ANALYSIS OF PURIFIED AAV WITH TSKgel SP-NPR
TSKgel
Higher DEAE-NPR
resolution and faster analysis on TSKgel DEAE-NPR
Analysis of purified AAV with TSKgel SP-NPR
540
504
587
434

458

20

15
267

mAU
234
213
184

10
192
123

5
124
104
89
80

0
64
57
51

-5

2 5 10 13 0 2.5 5 7.5 10
Minutes Minutes
Column:TSKgel TSKgel
Column: DEAE-NPR,DEAE-NPR,
4.6 mm 4.6mm ID cm
ID x 3.5 x 3.5cm, with
L, with guard
guard column, Column: TSKgel SP-NPR, 4.6mm ID x 3.5cm
column, 4.6mm ID x 0.5cm
4.6 mm ID x 0.5 cm L; Sample: Hae III digest of pBR322 DNA, (base pair Sample:
Column: TSKgel purified adeno-associated
SP-NPR, 4.6 mm ID x 3.5virus
cm L; Sample: purified adeno-
Sample: Hae III digest of pBR322 DNA,
number for each peak is indicated); Buffer A: 0.02 mol/L Tris-HCl, pH 9.0; associated virus; Elution: A.HEPES,
Elution: A. 50mmol/L 1mmol/L
50 mmol/L EDTA,
HEPES, 5mmol/L
1 mmol/L MgCl,
EDTA, pH 7.5;
5 mmol/L
(base pair number for each peak is indicated) B. 50mmol/L HEPES, 1mmol/L EDTA, 5mmol/L MgCl, pH 7.5 with
Buffer B: Buffer A plus 1.0 mol/L NaCl; Elution: 15 min linear gradient from MgCl, pH 7.5; B. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH
Buffer A: 0.02mol/L Tris-HCl, pH 9.0 0.5mol/L NaCl; linear gradient from 20% to 100% B in
48Buffer
% to 65
B: % buffer B; Flow
Buffer rate:1.5
A plus mL/min;
1.0mol/L Pressure: 14 Mpa; Temp.: 40 C;
NaCl 7.5 with 0.5 mol/L NaCl; linear gradient from 20 % to 100 % B in 10 column
10 column volumes
Detection:
Elution: UV @ 260
15minnmlinear gradient from 48% to 65% buffer B volumes; Flow rate: 1 mL/min; Detection: UV @ 280 nm
Flow Rate: 1mL/min
Flow Rate: 1.5mL/min Detection: UV @ 280nm
Pressure: 2000psi
Temperature: 40º C
Detection: UV @ 260nm
16 WWW.TOSOHBIOSCIENCE.DE

IEC
TSKgel SW SERIES
IEC

The silica-based ion exchange columns are typically used APPLICATIONS OF TSKgel SW ION EXCHANGE COLUMNS
in the separation of low molecular weight compounds such
as pharmaceuticals, nucleotides or small peptides. Silica- Silica-based Anion Exchange Columns
based particles are used in pore sizes of 125 nanometer
(2SW) and 250 nanometer (3SW) with either diethylami- TSKgel 2SW-type columns provide high performance sepa-
noethyl (DEAE) or carboxymethyl (CM) functionality. Bind- rations of small ionic solutes. High performance analyses
ing capacity for small to medium size proteins on TSKgel of small anionic species are best performed on small pore
DEAE-3SW is roughly double that of the DEAE-5PW due to silica-based anion exchangers, such as TSKgel DEAE-2SW.
the smaller pore size and larger surface area. This is demonstrated in Figure 20. The 250 nanometer pore
size TSKgel DEAE-3SW column is used for separating pep-
The increased solubility of the silica backbone at pH above tides, low MW proteins and DNA fragments.
pH 7.5 limits the use of silica based ion exchange columns
to acidic or neutral mobile phases. Silica-based Cation Exchange Columns

Silica-based cation exchangers are typically used in the

separation of low molecular weight compounds such as
pharmaceuticals, nucleotides, catecholamines, and small
peptides. For example, Figure 21 shows the separation of
nucleosides on TSKgel SP-2SW.

FIGURE 20 FIGURE 21

SEPARATION OF NUCLEOTIDES ON TSKgel DEAE-2SW Separation

SEPARATIONof OF
nucleosides by ion-exchange
NUCLEOSIDES chromatography
BY IEC ON TSKgel SP-2SW
Separation of nucleotides on TSKgel DEAE-2SW on TSKgel SP-2SW
A. pH 3.5 B. pH 4.25
4
1
2
2
5

3
3
2

1
3
inj. inj.

0 12 24 0 5 10 0 5 10
Minutes Minutes
Minutes
Column: TSKgel DEAE-2SW, 4.6mm ID x 25cm Column: TSKgel SP-2SW 4.6mm ID x 25cm
Column: TSKgel DEAE-2SW, 4.6 mm ID x 25 cm L; Sample: 1. AMP, 2. IMP,
Sample: 1. AMP, 2. IMP, 3. GMP, 4.ADP, 5. ATP Sample:
Column: TSKgelNucleoside
SP-2SW 4.6Standards:
mm ID x 25 1)cmGuanosine,
L 2) Cytidine, 3) Adenosine
3. GMP, 4.ADP, 5. ATP; Buffer A: ACN in 0.1 mol/L phosphate, pH 3.0, 20/80; Mobile
Sample:Phase: A) 0.1Standards:
Nucleoside mol/L sodium citrate - phosphoric
1) Guanosine, acid
2) Cytidine, 3) buffer, pH 3.5
Adenosine
Buffer A: ACN in 0.1mol/L phosphate, pH 3.0, 20/80
Buffer B)0.1
0.1mol/L
mol/Lsodium
sodiumcitrate
citrate - acetic acid buffer,
buffer,pH
pH4.25
BufferB:B:ACN inACN
0.5 mol/L phosphate,
in 0.5mol/L pH 3.0, pH
phosphate, 20/80;
3.0,Elution:
20/80 30 min linear Mobile Phase: A) - phosphoric acid 3.5
gradient Flow Rate: 0.75 mL/min
Elution:from buffer A tolinear
30min B; Flow rate: 1.0from
gradient mL/min; Detection:
buffer A to B UV @ 260 nm B) 0.1 mol/L sodium citrate - acetic acid buffer, pH 4.25
Temperature: 23°C
Flow Rate: 1.0mL/min Flow rate: 0.75 mL/min; Temperature 23 °C; Detection: UV @ 260 nm
Detection: UV detection @ 260nm
Detection: UV @ 260nm
 TOSOH BIOSCIENCE ANALYSIS WWW.TOSOHBIOSCIENCE.DE

1

TOSOH BIOSCIENCE GMBH


2
TOSOH BIOSCIENCE LLC
3
TOSOH CORPORATION
TSKgel ANION EXCHANGE COLUMNS TSKgel CATION EXCHANGE COLUMNS
IM LEUSCHNERPARK 4
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WWW.TOSOHBIOSCIENCE.DE WWW.SEPARATIONS.US.TOSOHBIOSCIENCE.COM WWW.TOSOHBIOSCIENCE.COM Part # Description ID Length Particle Number Flow rate (mL/min) Maximum Part # Description ID Length Particle Number Flow rate (mL/min) Maximum
(mm) (cm) size (µm) theoretical Range pressure (mm) (cm) size (µm) theoretical Range pressure
plates drop (MPa) plates drop (MPa)
TSKgel GLASS COLUMNS: POLYMER-BASED TSKgel GLASS COLUMNS: POLYMER-BASED
WITH A GLOBAL PERSPECTIVE. 0013061 DEAE-5PW Glass, 100 nm 5.0 5.0 10 ≥ 700 0.5 - 0.8 1.5 0014012 CM-5PW Glass, 100 nm 20.0 15.0 13 ≥ 2,500 4.0 - 6.0 1.5
0008802 DEAE-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 1.0
Tosoh Bioscience is a leading manufacturer in the field of liquid 0014016 DEAE-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5
0013062
0008803
SP-5PW Glass, 100 nm
SP-5PW Glass, 100 nm
5.0
8.0
5.0
7.5
10
10
≥ 700
≥ 1,300
0.5
0.5
-
-
0.8
1.0
1.5
1.0
chromatography. The portfolio of over 500 specialty products 0018386 SuperQ-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0014017 SP-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5

encompasses instruments for size exclusion/gel permeation TSKgel PEEK COLUMNS: POLYMER-BASED
0019685 BioAssist Q, 400 nm 4.6 5.0 10 ≥ 500 0.3 - 1.0 2.5
TSKgel PEEK COLUMNS: POLYMER-BASED

chromatography and a comprehensive line of media and prepacked 1

0021410 BioAssist Q, 400 nm 10.0 10.0 13 ≥ 500 1.0 - 5.0 2.5 0019686
0021411
BioAssist S, 130 nm
BioAssist S, 130 nm
4.6
10.0
5.0
10.0
7
13
≥ 1,500
≥ 3,000
0.3 - 0.8
1.0 - 5.0
2.5
2.5
(U)HPLC columns for all common modes of liquid chromatogra- 2 3
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
0021960 Q-STAT, nonporous 3.0 3.5 10 > 200 1.0 - 2.0 10.0
phy. Over the last 40 years, TSKgel SW columns have become the 4
0021961 Q-STAT, nonporous 4.6 10.0 7 > 4,000 0.5 - 1.4 10.0 0021965 CM-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0021962 DNA-STAT, nonporous 4.6 10.0 5 > 4,000 0.3 - 0.6 15.0 0021966 CM-STAT, nonporous 4.6 10.0 7 ≥ 2,000 0.5 - 1.0 10.0
worldwide industry standard for size exclusion chromatography of 0013075 DEAE-NPR, nonporous 4.6 3.5 2.5 ≥ 1,300 1.0 - 1.5 20.0
0021963 SP-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0018249 DNA-NPR, nonporous 4.6 7.5 2.5 ≥ 6,000 0.5 - 1.0 30.0
biomolecules. 5 0018757 DEAE-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.5 0021964 SP-STAT, nonporous 4.6 10.0 7 ≥ 200 0.5 - 1.4 10.0
0007164 DEAE-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5 0013068 CM-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007574 DEAE-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5 0018758 SP-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.0
Tosoh manufacturing sites in Japan provide products to the sales 0007930
0018257
DEAE-5PW, 100 nm
SuperQ-5PW, 100 nm
55.0
7.5
20.0
7.5
20
10
≥ 1,500
≥ 1,300
20.0
0.5
-
-
40.0
1.0
0.4
2.0
0007161 SP-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007575 SP-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5
and support subsidiaries in the U.S. and Europe, ensuring full global 0018387 SuperQ-5PW, 100 nm
0008639 Sugar AXI, 6 nm
21.5
4.6
15.0
15.0
13
8
≥ 3,000
≥ 3,700
4.0
0.2
-
-
6.0
0.4
2.0
3.0 0007934 SP-5PW, 100 nm 55.0 20.0 20 ≥ 1,500 20.0 - 40.0. 0.4
coverage. Our technical specialists in the European Headquarters 0008640 Sugar AXG, 6 nm 4.6 15.0 10 ≥ 2,700 0.2 - 0.5 2.0 0013076
0007156
SP-NPR, nonporous
SCX (Na+), 6 nm
4.6
6.0
3.5
15.0
2.5
5
≥ 1,300
≥ 2,000
1.0 - 1.5
0.5 - 1.0
20.0
15.0
0007157 SAX, 6 nm 6.0 15.0 5 ≥ 2,000 0.5 - 1.0 15.0
provide assistance in developing HPLC applications or purification 4
TOSOH BIOSCIENCE SHANGHAI CO. LTD.
5
TOSOH ASIA PTE. LTD.
0007158 SCX (H+) 6 nm 7.8 30.0 5 ≥ 12,000 0.5 - 1.0 5.0
TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
methods, in up-scaling, or packing process columns. We offer chro- ROOM 101, INNOV TOWER, 63 MARKET STREET #10-03
BLOCK A, NO 1801 HONGMEI ROAD BANK OF SINGAPORE CENTRE 0018761 DEAE-2SW, 12.5 nm ­ 2.0 25.0 5 ≥ 5,000 0.12 - 0.17 13.0 TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
XU HUI DISTRICT SINGAPORE 048942, SINGAPORE 0007168 DEAE-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0 0007165 SP-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
matographic workshops, on-site training, and are the sole sponsor SHANGHAI, 200233, CHINA
T +86 21 3461 0856 T +65 6226 5106
0007163 DEAE-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0007167 CM-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
0007162 CM-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0
of the HIC/RPC Bioseparation Conference series. F +86 21 3461 0858 F +65 6226 5215
INFO@TOSOH.COM.CN INFO.TSAS@TOSOH.COM TSKgel GUARD COLUMN PRODUCTS
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0017088 DEAE-NPR Guard column 4.6 0.5 5 For P/N 0013075 GUARD COLUMN PRODUCTS
0018253 DNA-NPR Guard column 4.6 0.5 2.5 For P/N 0018249
TOSOH HISTORY 0018388 SuperQ-5PW Guardgel Kit 20 For P/N 0018257
0013069 CM-5PW Guardgel Kit 10 For P/N 0013068
1935 FOUNDING OF TOYO SODA MANUFACTURING CO., LTD. 0007211 SP-5PW Guardgel Kit 20 For P/N 0007161
0007210 DEAE-5PW Guardgel Kit 20 For P/N 0007164
1936 OPERATION OF NANYO MANUFACTURING COMPLEX BEGINS 0008807 SP-5PW Guardgel Kit, Glass 20 For P/Ns 0013062 and 0008803
0008806 DEAE-5PW Guardgel Kit, Glass 20 For P/Ns 0013061 and 0008802
0016093 SP-5PW Prep Guardgel Kit 20 For P/N 0007575
1971 SCIENTIFIC INSTRUMENTS DIVISION FORMED, FIRST GPC COLUMN USING TSKgel DEVELOPED BY TOSOH 0014466 DEAE-5PW Guardcol., Glass 20.0 2.0 13 For P/N 0014016
0007932 SP-5PW Guard column 45.0 5.0 20 For P/N 0007934
1974 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN PLANT IS COMPLETED 0016092 DEAE-5PW Prep Guardgel Kit 20 For P/N 0007574
0007650 CM-SW Guardgel Kit 20 For P/Ns 0007167 and 0007162
1979 TOSOH DEVELOPS TOYOPEARL MEDIA 0007928 DEAE-5PW Guard column 45.0 5.0 20 For P/N 0007930
1983 TOSOH DEVELOPS HYDROPHOBIC INTERACTION MEDIA 0007648 DEAE-SW Guardgel Kit 10 For P/Ns 0007168 and 0007163
1987 TOSOHAAS US OPERATIONS FORMED IN MONTGOMERYVILLE 0019308 Guard cartridge holder 2.0 1.5 For all 2 mm ID guard cartridges
1989 TOSOHAAS GMBH OPERATIONS FORMED IN STUTTGART

1995 TOSOH NANYO GEL FACILITY RECEIVES ISO 9001

2002/2003 ALL TOSOH AFFILIATED SCIENTIFIC & DIAGNOSTIC SYSTEM RELATED COMPANIES IN EUROPE ARE UNIFIED UNDER THE NAME TOSOH BIOSCIENCE.

2008 EcoSEC, THE 7TH GENERATION GPC SYSTEM IS INTRODUCED GLOBALLY

2010 TOSOH CELEBRATES ITS 75TH YEAR IN BUSINESS WITH THE OPENING OF FIVE NEW PLANTS, AND CONTINUED RAPID EXPANSION IN CHINA

2011 TOSOH BIOSCIENCE CELEBRATES 40 YEARS OF OPERATION

2012 TOSOH RELEASES FIRST TOYOPEARL MIXED-MODE RESIN TOYOPEARL MX-Trp-650M

2013 TOSOH RELEASES A HIGH CAPACITY PROTEIN A CHROMATOGRAPHY RESIN

2014 TOSOH BIOSCIENCE GMBH CELEBRATES ITS 25TH ANNIVERSARY IN STUTTGART

2015 TOSOH BIOSCIENCE SUCCESSFULLY MOVES ITS SALES & MARKETING OFFICES TO GRIESHEIM, DARMSTADT
 TOSOH BIOSCIENCE ANALYSIS WWW.TOSOHBIOSCIENCE.DE

1

TOSOH BIOSCIENCE GMBH


2
TOSOH BIOSCIENCE LLC
3
TOSOH CORPORATION
TSKgel ANION EXCHANGE COLUMNS TSKgel CATION EXCHANGE COLUMNS
IM LEUSCHNERPARK 4
64347 GRIESHEIM


3604 HORIZON DRIVE,
SUITE 100
3-8-2 SHIBA, MINATO-KU
TOKYO 105-8623
ORDERING INFORMATION ORDERING INFORMATION
GERMANY KING OF PRUSSIA, PA 19406, USA JAPAN

ABOUT US
T + 49 (0) 6155 70437 00 T +1 484 805 1219 T +81 3 5427 5118
F + 49 (0) 6155 83579 00 F +1 610 272 3028 F +81 3 5427 5198 ORDERING INFORMATION ORDERING INFORMATION
INFO.TBG@TOSOH.COM INFO.TBL@TOSOH.COM INFO@TOSOH.CO.JP
WWW.TOSOHBIOSCIENCE.DE WWW.SEPARATIONS.US.TOSOHBIOSCIENCE.COM WWW.TOSOHBIOSCIENCE.COM Part # Description ID Length Particle Number Flow rate (mL/min) Maximum Part # Description ID Length Particle Number Flow rate (mL/min) Maximum
(mm) (cm) size (µm) theoretical Range pressure (mm) (cm) size (µm) theoretical Range pressure
plates drop (MPa) plates drop (MPa)
TSKgel GLASS COLUMNS: POLYMER-BASED TSKgel GLASS COLUMNS: POLYMER-BASED
WITH A GLOBAL PERSPECTIVE. 0013061 DEAE-5PW Glass, 100 nm 5.0 5.0 10 ≥ 700 0.5 - 0.8 1.5 0014012 CM-5PW Glass, 100 nm 20.0 15.0 13 ≥ 2,500 4.0 - 6.0 1.5
0008802 DEAE-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 1.0
Tosoh Bioscience is a leading manufacturer in the field of liquid 0014016 DEAE-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5
0013062
0008803
SP-5PW Glass, 100 nm
SP-5PW Glass, 100 nm
5.0
8.0
5.0
7.5
10
10
≥ 700
≥ 1,300
0.5
0.5
-
-
0.8
1.0
1.5
1.0
chromatography. The portfolio of over 500 specialty products 0018386 SuperQ-5PW Glass, 100 nm 8.0 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0014017 SP-5PW Glass, 100 nm 20.0 15.0 13 ≥ 3,000 4.0 - 6.0 1.5

encompasses instruments for size exclusion/gel permeation TSKgel PEEK COLUMNS: POLYMER-BASED
0019685 BioAssist Q, 400 nm 4.6 5.0 10 ≥ 500 0.3 - 1.0 2.5
TSKgel PEEK COLUMNS: POLYMER-BASED

chromatography and a comprehensive line of media and prepacked 1

0021410 BioAssist Q, 400 nm 10.0 10.0 13 ≥ 500 1.0 - 5.0 2.5 0019686
0021411
BioAssist S, 130 nm
BioAssist S, 130 nm
4.6
10.0
5.0
10.0
7
13
≥ 1,500
≥ 3,000
0.3 - 0.8
1.0 - 5.0
2.5
2.5
(U)HPLC columns for all common modes of liquid chromatogra- 2 3
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
TSKgel STAINLESS STEEL COLUMNS: POLYMER-BASED
0021960 Q-STAT, nonporous 3.0 3.5 10 > 200 1.0 - 2.0 10.0
phy. Over the last 40 years, TSKgel SW columns have become the 4
0021961 Q-STAT, nonporous 4.6 10.0 7 > 4,000 0.5 - 1.4 10.0 0021965 CM-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0021962 DNA-STAT, nonporous 4.6 10.0 5 > 4,000 0.3 - 0.6 15.0 0021966 CM-STAT, nonporous 4.6 10.0 7 ≥ 2,000 0.5 - 1.0 10.0
worldwide industry standard for size exclusion chromatography of 0013075 DEAE-NPR, nonporous 4.6 3.5 2.5 ≥ 1,300 1.0 - 1.5 20.0
0021963 SP-STAT, nonporous 3.0 3.5 10 ≥ 200 1.0 - 2.0 10.0
0018249 DNA-NPR, nonporous 4.6 7.5 2.5 ≥ 6,000 0.5 - 1.0 30.0
biomolecules. 5 0018757 DEAE-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.5 0021964 SP-STAT, nonporous 4.6 10.0 7 ≥ 200 0.5 - 1.4 10.0
0007164 DEAE-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5 0013068 CM-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007574 DEAE-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5 0018758 SP-5PW, 100 nm ­ 2.0 7.5 10 ≥ 1,300 0.05 - 0.10 1.0
Tosoh manufacturing sites in Japan provide products to the sales 0007930
0018257
DEAE-5PW, 100 nm
SuperQ-5PW, 100 nm
55.0
7.5
20.0
7.5
20
10
≥ 1,500
≥ 1,300
20.0
0.5
-
-
40.0
1.0
0.4
2.0
0007161 SP-5PW, 100 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 1.5
0007575 SP-5PW, 100 nm 21.5 15.0 13 ≥ 3,000 4.0 - 6.0 2.5
and support subsidiaries in the U.S. and Europe, ensuring full global 0018387 SuperQ-5PW, 100 nm
0008639 Sugar AXI, 6 nm
21.5
4.6
15.0
15.0
13
8
≥ 3,000
≥ 3,700
4.0
0.2
-
-
6.0
0.4
2.0
3.0 0007934 SP-5PW, 100 nm 55.0 20.0 20 ≥ 1,500 20.0 - 40.0. 0.4
coverage. Our technical specialists in the European Headquarters 0008640 Sugar AXG, 6 nm 4.6 15.0 10 ≥ 2,700 0.2 - 0.5 2.0 0013076
0007156
SP-NPR, nonporous
SCX (Na+), 6 nm
4.6
6.0
3.5
15.0
2.5
5
≥ 1,300
≥ 2,000
1.0 - 1.5
0.5 - 1.0
20.0
15.0
0007157 SAX, 6 nm 6.0 15.0 5 ≥ 2,000 0.5 - 1.0 15.0
provide assistance in developing HPLC applications or purification 4
TOSOH BIOSCIENCE SHANGHAI CO. LTD.
5
TOSOH ASIA PTE. LTD.
0007158 SCX (H+) 6 nm 7.8 30.0 5 ≥ 12,000 0.5 - 1.0 5.0
TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
methods, in up-scaling, or packing process columns. We offer chro- ROOM 101, INNOV TOWER, 63 MARKET STREET #10-03
BLOCK A, NO 1801 HONGMEI ROAD BANK OF SINGAPORE CENTRE 0018761 DEAE-2SW, 12.5 nm ­ 2.0 25.0 5 ≥ 5,000 0.12 - 0.17 13.0 TSKgel STAINLESS STEEL COLUMNS: SILICA-BASED
XU HUI DISTRICT SINGAPORE 048942, SINGAPORE 0007168 DEAE-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0 0007165 SP-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
matographic workshops, on-site training, and are the sole sponsor SHANGHAI, 200233, CHINA
T +86 21 3461 0856 T +65 6226 5106
0007163 DEAE-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0 0007167 CM-2SW, 12.5 nm 4.6 25.0 5 ≥ 5,000 0.6 - 0.8 15.0
0007162 CM-3SW, 25 nm 7.5 7.5 10 ≥ 1,300 0.5 - 1.0 2.0
of the HIC/RPC Bioseparation Conference series. F +86 21 3461 0858 F +65 6226 5215
INFO@TOSOH.COM.CN INFO.TSAS@TOSOH.COM TSKgel GUARD COLUMN PRODUCTS
WWW.SEPARATIONS.ASIA.TOSOHBIOSCIENCE.COM WWW.TOSOHASIA.COM
0017088 DEAE-NPR Guard column 4.6 0.5 2.5 For P/N 0013075 GUARD COLUMN PRODUCTS
0018253 DNA-NPR Guard column 4.6 0.5 2.5 For P/N 0018249
TOSOH HISTORY 0018388 SuperQ-5PW Guardgel Kit 20 For P/N 0018257
0013069 CM-5PW Guardgel Kit 10 For P/N 0013068
1935 FOUNDING OF TOYO SODA MANUFACTURING CO., LTD. 0007211 SP-5PW Guardgel Kit 20 For P/N 0007161
0007210 DEAE-5PW Guardgel Kit 20 For P/N 0007164
1936 OPERATION OF NANYO MANUFACTURING COMPLEX BEGINS 0008807 SP-5PW Guardgel Kit, Glass 20 For P/Ns 0013062 and 0008803
0008806 DEAE-5PW Guardgel Kit, Glass 20 For P/Ns 0013061 and 0008802
0016093 SP-5PW Prep Guardgel Kit 20 For P/N 0007575
1971 SCIENTIFIC INSTRUMENTS DIVISION FORMED, FIRST GPC COLUMN USING TSKgel DEVELOPED BY TOSOH 0014466 DEAE-5PW Guardcol., Glass 20.0 2.0 13 For P/N 0014016
0007932 SP-5PW Guard column 45.0 5.0 20 For P/N 0007934
1974 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN PLANT IS COMPLETED 0016092 DEAE-5PW Prep Guardgel Kit 20 For P/N 0007574
0007650 CM-SW Guardgel Kit 20 For P/Ns 0007167 and 0007162
1979 TOSOH DEVELOPS TOYOPEARL MEDIA 0007928 DEAE-5PW Guard column 45.0 5.0 20 For P/N 0007930
1983 TOSOH DEVELOPS HYDROPHOBIC INTERACTION MEDIA 0007648 DEAE-SW Guardgel Kit 10 For P/Ns 0007168 and 0007163
1987 TOSOHAAS US OPERATIONS FORMED IN MONTGOMERYVILLE 0019308 Guard cartridge holder 2.0 1.5 For all 2 mm ID guard cartridges
1989 TOSOHAAS GMBH OPERATIONS FORMED IN STUTTGART

1995 TOSOH NANYO GEL FACILITY RECEIVES ISO 9001

2002/2003 ALL TOSOH AFFILIATED SCIENTIFIC & DIAGNOSTIC SYSTEM RELATED COMPANIES IN EUROPE ARE UNIFIED UNDER THE NAME TOSOH BIOSCIENCE.

2008 EcoSEC, THE 7TH GENERATION GPC SYSTEM IS INTRODUCED GLOBALLY

2010 TOSOH CELEBRATES ITS 75TH YEAR IN BUSINESS WITH THE OPENING OF FIVE NEW PLANTS, AND CONTINUED RAPID EXPANSION IN CHINA

2011 TOSOH BIOSCIENCE CELEBRATES 40 YEARS OF OPERATION

2012 TOSOH RELEASES FIRST TOYOPEARL MIXED-MODE RESIN TOYOPEARL MX-Trp-650M

2013 TOSOH RELEASES A HIGH CAPACITY PROTEIN A CHROMATOGRAPHY RESIN

2014 TOSOH BIOSCIENCE GMBH CELEBRATES ITS 25TH ANNIVERSARY IN STUTTGART

2015 TOSOH BIOSCIENCE SUCCESSFULLY MOVES ITS SALES & MARKETING OFFICES TO GRIESHEIM, DARMSTADT
 TOSOH BIOSCIENCE ANALYSIS PROCESS INSTRUMENTATION

FURTHER INFORMATION

PRINCIPLES OF CHROMATOGRAPHY

To get an overview about the whole range

of TSKgel columns and small TOYOPEARL
and TSKgel bulk media, please request our
For a complete overview of all TOYOPEARL
and TSKgel bulk media, refer to our
Chromatographic Process Media Catalog.
ION EXCHANGE COLUMNS The analysis, isolation, and purifi­cation of biomolecules can be
accomplished by a number of chromatographic modes. Each
Chromatography Catalog.
mode is based on specific physical, chemical, or biological inter-
actions between the sample biomolecule and packing material.

Tosoh Bioscience offers a comprehensive line of TSKgel and

TOYOPEARL media and pre-packed TSKgel columns for all com-
mon modes of liquid chromatography.

The various modes of chromato­graphy involve separations that

are based on specific features of the target or sample, like size,
charge, hydropho­­bi­city, function or specific content of the mol-
ecule. To find out more about general principles of liquid chro-
matography and on how each of them works, visit us on our
YouTube channel.

For a deeper insight into applications and questions related to the practical use of TSKgel and
TOYOPEARL, check out the website www.tosohbioscience.de

Our technical experts are happy to discuss your specific separation needs by phone: +49 (0)6155-70437-36
or mail: techsupport.tbg@tosoh.com

TOSOH BIOSCIENCE

Im Leuschnerpark 4 64347 Griesheim, Germany

Tel: +49 6155-7043700 Fax: +49 6155-8357900 ION EXCHANGE CHROMATOGRAPHY
info.tbg@tosoh.com www.tosohbioscience.de
B14LXXA
 TOSOH BIOSCIENCE ANALYSIS PROCESS INSTRUMENTATION

FURTHER INFORMATION

PRINCIPLES OF CHROMATOGRAPHY

To get an overview about the whole range

of TSKgel columns and small TOYOPEARL
and TSKgel bulk media, please request our
For a complete overview of all TOYOPEARL
and TSKgel bulk media, refer to our
Chromatographic Process Media Catalog.
ION EXCHANGE COLUMNS The analysis, isolation, and purifi­cation of biomolecules can be
accomplished by a number of chromatographic modes. Each
Chromatography Catalog.
mode is based on specific physical, chemical, or biological inter-
actions between the sample biomolecule and packing material.

Tosoh Bioscience offers a comprehensive line of TSKgel and

TOYOPEARL media and pre-packed TSKgel columns for all com-
mon modes of liquid chromatography.

The various modes of chromato­graphy involve separations that

are based on specific features of the target or sample, like size,
charge, hydropho­­bi­city, function or specific content of the mol-
ecule. To find out more about general principles of liquid chro-
matography and on how each of them works, visit us on our
YouTube channel.

For a deeper insight into applications and questions related to the practical use of TSKgel and
TOYOPEARL, check out the website www.tosohbioscience.de

Our technical experts are happy to discuss your specific separation needs by phone: +49 (0)6155-70437-36
or mail: techsupport.tbg@tosoh.com

TOSOH BIOSCIENCE

Im Leuschnerpark 4 64347 Griesheim, Germany

Tel: +49 6155-7043700 Fax: +49 6155-8357900 ION EXCHANGE CHROMATOGRAPHY
info.tbg@tosoh.com www.tosohbioscience.de
B16L01A