Column Protection Guide

Version 0610

Includes:
• Mobile Phase Limitations
• Column Storage Tips
• Column Protection
Devices
column protection guide
TABLE OF CONTENTS
INTRODUCTION TO COLUMN CARE AND USAGE 1-4
Selecting the Right Tubing and Fittings
Column Installation

PART I - SILICA-BASED & TWIN™ TECHNOLOGY

COLUMNS 4-7
Running Parameters Scaling Up/Scaling Down
Mobile Phase Considerations Column Storage
Stationary Phase Considerations Column Cleaning Procedures
Backpressure and Flow Rates
Part II - SFC
(Supercritical fluid chromatography) 7
Running Parameters Cleaning Procedure
Equilibrating Column Column Storage
Mobile Phase Considerations
PART III -AXIA PREPARATIVE COLUMNS 8
Running Parameters Cleaning Procedure
Mobile Phase Considerations Column Storage
PART IV - Lux CHIRAL COLUMNS 8-9
Running Parameters Extending Lifetime and Reconditioning
Mobile Phase Considerations Column Storage
Part V - Chirex Chiral Columns 10
Running Parameters
Mobile Phase Considerations

PART VI - BioSep-SEC-S Columns 10

Running Parameters
Mobile Phase Considerations
Cleaning Procedure
Column Storage

PART VII - REZEX POLYMER-BASED COLUMNS 11-15

Running Parameters
Mobile Phase Considerations
Cleaning Procedure
Table of Specifications and Operating Parameters

PART vIII - PolySep-GFC-P Columns 16

Running Parameters
Mobile Phase Considerations
Cleaning Procedure
Column Storage

PART IX - PHENOGEL GPC COLUMNS 17, 20-21

Specifications Solvent Switching Considerations
Sample Considerations Solvent Compatibility Chart
Column Storage
Solvent Miscibility Table 18-19
PART X - POLYMERX RP COLUMNS 22
Specifications Cleaning Procedure
Running Parameters Column Storage
Mobile Phase Considerations
PART XI - HPLC COLUMN PROTECTION 22-29
and PERFORMANCE TESTING
Phenex Syringe Filters 22-24
Membrane Filters Order List Guide 22-23
Phenex Disposable Centrifugal Filter Units 24
SecurityGuard Guard Cartridge System 25-29
HPLC System Test Kit 30
Column Performance Check Standards 30-33

PART XII - SOLID PHASE EXTRACTION (SPE) 34

PART XIII - HPLC ACCESSORIES 35
PHENOMENEX WARRANTY 36
COLUMN PERFORMANCE RECORD 37
 INTRODUCTION

Every Phenomenex HPLC column is a precision product

which, though delicate, will provide excellent performance,
reproducibility and column lifetime if cared for properly. The
information and recommendations contained in this manual are
designed to guide you in the care and use of your column, but
should not be considered absolute. Please follow the instructions
herein to maximize column performance and lifetime. Should you
have any questions, please contact your Phenomenex Technical
Representative or local distributor.

UPON RECEIPT OF THE COLUMN

• Verify the column you received is the column
you ordered
• Check the column for physical damage which
may have occurred during shipping
• Test the column immediately to verify
performance and quality
• All columns are shipped in the testing solvent,
unless otherwise specified
Each Phenomenex manufactured HPLC column is individually
packed and tested to ensure high column quality. Every column
is supplied with its Test Chromatogram and a Specification
Sheet which indicates column serial number and identity, testing
conditions and operating parameters.
The warranty period begins upon receipt of the column.
Testing is especially important if the column is to be placed
in storage. Test the column using the same conditions in
the test chromatogram. Use the formulae in Figure 1 to
determine column efficiency and peak asymmetry.
Chromatographic performance depends on the entire system,
not just the column. Columns are QC tested using optimum
conditions to minimize bandspreading from “extra column
effects.” Most variations from the Phenomenex test data are
due to extra-column effects created by the design of your system
(i.e., injector, flow cell, connecting tubing, etc.). If you have any
questions regarding your test results or the column quality, or if
there are signs of damage, CONTACT PHENOMENEX OR YOUR
LOCAL DISTRIBUTOR IMMEDIATELY.

Figure 1

Tangent lines
are used in
determining
Peak width at peak width
at base (W) 10 % peak
half height (W½) W½ height
is actual width of
peak at half-height
inject

A B

A = peak from @ 10 % to dropline

W B = dropline to peak tail @ 10 %
Retention time for:
tO unretained peak
1
R1 peak 1
t
R2 peak 2

Peak Asymmetry Column Efficiency

( )
B t 2
As = N = 5.54 R1
A W½ W½

Formulae for calculating efficiency and peak asymmetry

© 2010 Phenomenex, Inc. All rights reserved.

1
 SELECTING THE RIGHT TUBING AND
FITTINGS
The tubing and fittings on an HPLC system contribute to system
dead volume. If not minimized, dead volume can lead to band
broadening and peak degradation. Please use the following
guideline to keep system dead volume to a minimum and to help
ensure optimum column performance.
TUBING
The choice of tubing material is based on its chemical
resistivity, application and HPLC system considerations
(i.e. flow rate, backpressure, etc). Please refer to Tables 1-3
for specifics.
TUBING DIAMETER
Table 1
High Pressure Tubing:

1/16 in. OD x 0.010 in. ID

Inlet/Outlet Low Pressure Tubing:

1/16 in. OD x 0.030 in. ID 1/8 in. OD x 0.062 in. ID

TUBING COMPATIBILITY
Table 2
Stainless Steel AVOID high concentrations
(Type 316) of acids or halogenated salts

PEEK AVOID 100 % THF,

(biocompatible) chlorinated solvents, high
concentrations of acids
Titanium Compatible with nearly all
(biocompatible) chemicals

TUBING APPLICATIONS
Table 3
Tubing ID Column IDs Typical Flow Rates
(Inch) (mm) (mL/min)

0.002 0.30 (Fused Silica) 0.001 - 0.02

0.005 1.0 (Stainless Steel) 0.02 - 0.1
0.007 2.0 - 4.6 0.2 - 2.0
0.010 3.2 - 7.8 0.5 - 5.0
0.020 10.0 - 21.2 2.0 - 50.0
0.040 21.2 - 100.0 10.0 - 200.0

2
Fittings
All Phenomenex column end-fittings are female inverted (internal
type) with 10-32 type threading:
• The end-fitting can fit any 1/16 in. OD tubing (see page 2 for
tubing considerations)
• A 10-32 threaded male nut and ferrule or a polymeric
fingertight male nut* is used to swage or tighten the tubing
onto the fitting (see Figure 2)

Figure 2

Polymeric
Fingertight
Male Nut
32 threads per inch
No. 10 or 0.190 in. diameter
Ferrule

1/16 in. OD
Tubing Male Nut
(0.010 in. ID) /4 in. OD Standard Analytical
1
Seating depth of the nib
(250 x 4.6 mm ID)
is the critical dimension
10-32 Threaded Male Nut Female / Inverted (internal)
and Ferrule Column End-fitting

Installation
Figure 3
Considerations:
• The shape of the swaged
ferrule can differ between
manufacturers. For
Phenomenex columns, you
may use Phenomenex or
Valco type ferrules.
• VERY IMPORTANT:
The seating depth of
the nib (Figure 2) for
Phenomenex columns is
0.080 in. Tubing MUST be
seated all the way down
into the column end-fitting.
Failure to do so will result
in having a small mixing
chamber at the top or
bottom of the column.
This will lead to degraded
chromatography.
*Polymeric fingertight
fittings are easy to use.
They come in one piece,
require NO tools for
attachment and easily
conform to the shape of
the column end-fitting.

3
 Column Installation

IT IS HIGHLY RECOMMENDED THAT YOU READ THIS

GUIDE FOR SPECIFIC COLUMN CONSIDERATIONS BEFORE
PROCEEDING WITH THE INSTALLATION (PARTS I-X)
• Flush HPLC pump and line thoroughly with filtered and
degassed mobile phase (without any buffers). Make
sure there are no air bubbles in the system.
• Connect the column to the injector corresponding to the
direction of the flow label (located on the column). Leave
the outlet of the column unattached.
• Set pump to flow at 0.1 mL/min (or lowest setting) and
increase to normal flow rate over 5 minutes.
• Stop flow when there is a free flow of solvent from the
column outlet, wipe the end and attach to the detector
• Equilibrate the column by passing approximately 10-30
column volumes of mobile phase at normal flow rate.
• For those columns that can be used under reversed-
phase or normal phase conditions (i.e., -CN or -NH2),
flush with 20-30 column volumes of IPA or THF as the
intermediate solvent when switching from
reversed-phase to normal phase modes, or vice versa.

PART i - SILICA-BASED & TWIN™

TECHNOLOGY COLUMNS
RUNNING PARAMETERS
• Keep backpressures below 3500 psi (245 bar) [maximum
5000 psi] unless otherwise specified in Parts I-X
• Avoid any sudden pressure changes
• If high backpressure is observed, reverse flush the column
(do not try this on other manufacturers’ columns)
• Use a backpressure regulator if you are experiencing out-
gassing problems in the detector cell.
• Maximum operating temperature is 60 °C for all
Phenomenex silica-based reversed phase columns.

Mobile Phase Considerations

• Use only HPLC grade solvents and water
• Use only highest purity chemicals and reagents
• Filter and degas all mobile phases prior to use
• Make sure solvents are miscible
Trace impurities can dramatically degrade HPLC columns. When
changing to a different mobile phase, make sure the solvents
and/or buffers are miscible (see Table 11). Using solvents that
are immiscible with the solvent in the column can permanently
damage the column. Salt and buffer precipitation from the
mobile phase can permanently damage the column. Always
check sample solubility and if possible use the mobile phase as
the diluent (sample solvent).

Stationary Phase Considerations

• Maintain pH between 2.0 and 8.0*
• Use presaturator columns and guard columns
• Avoid aldehydes and ketones with amino columns
Silica-based columns are pH sensitive. Low pH (< 2.0) will
hydrolyze the bonded phase (strip off the functional groups) and
high pH (> 8.0) will dissolve the silica. If the mobile phase pH is
near 2.0 or 8.0, use a presaturator column.

*Consult Phenomenex for columns that have extended pH ranges.

4
backpressure and flow rates
To maximize column life, flow rates should be adjusted to keep
pressures below 3500 psi.
Table 4
Particle Internal Typical Flow Typical Pressure(psi)
Size µm Diameter(mm) Rate(mL/min) 150 mm* 250 mm*
3 | 4.6 | 0.5 | 985 | 1640
5 1.0 0.1 1500 2500
5 2.0 0.2 750 1250
5 3.0 0.5 732 1226
5 4.6 1.0 710 1180
5 10.0 5.0 750 1250
10 4.6 2.0 355 590
10 21.2 20.0 170 280
* column length
Columns can be operated at any flow rate that is consistent with
the backpressure limitations described above. Flow rates should
be optimized to provide the best efficiency for your sample.

Scaling Up/Scaling Down

Adjusting flow rates for different column internal diameters
is straightforward. To keep the retention times constant, the
flow rates and loading capacity must be adjusted according to
the column’s internal diameter. Assuming column length does
not change:
(radius column B) 2
X = Scale Factor =
(radius column A) 2

From a 4.6 mm ID column some approximate scaling factors

are:
Table 5
Internal Diameter Scaling Factor
1.0 mm 0.05x
2.0 mm 0.2x
3.0 mm 0.5x
10.0 mm 5x
21.2 mm 21x
HPLC columns running water-free, flammable organic
solvents (e.g., normal phase, chiral, GPC) can generate
static electricity and should be properly grounded to avoid
a potentially dangerous electrical discharge.

Column Storage
• Column storage conditions affect column lifetime
• Never store columns with buffers
• Flush with 5 column volumes of mobile phase without
buffer to remove any buffers or salts
Storage Conditions for Silica-Based HPLC Columns:

Table 6
Column Type Storage Solvent
Reversed Phase 65 % Acetonitrile/
C18, C12, C8, C4, C2, C1, 35 % Water
Phenyl, PFP
Normal Phase
Silica, CN, NH2, PAC, Isopropanol or Hexane
Diol Alumina
Ion-Exchange
Methanol*
SAX, SCX, WAX, WCX
Size-Exclusion 0.05 % NaN3 in water or
Diol 10 % methanol
HILIC 80 % Acetonitrile/
Luna HILIC 20 % Water
*Flush column with 50 mL HPLC grade water prior to storage solvent 5
 Column Cleaning Procedures
The following conditions apply to Phenomenex silica-based
columns with the exception of chiral columns (see Parts IV
and V):
• Before starting any kind of cleaning procedure, make sure
your in-column solvent or mobile phase is miscible with the
recommended cleaning solvent(s).
• Flow rates should be 1/5 – 1/2 of the typical flow rate.
To estimate the column volume, use the following
equation: V= r 2L V = column volume in mL
r = column radius in cm
L = column length in cm
UNBONDED SILICA
Rinse with 10 Column Volumes each of:
• Hexane
• Methylene Chloride
• Isopropanol
• Methylene Chloride
• Mobile Phase
Water Removal Procedure:
Flush column with 30 mL 2.5 % 2,2-dimethoxy-propane and
2.5 % glacial acetic acid in Hexane
BONDED NORMAL PHASE (CN, NH2, Diol, PAC)
Rinse with 10 Column Volumes each of:
• Chloroform
• Isopropanol
• Methylene Chloride
• Mobile Phase
Exception: Luna Amino in reversed phase mode.
HILIC
Rinse with 10 Column Volumes each of:
• 95 % Water/5 % Acetonitrile (for buffer removal)
• 95 % 100 mM Ammonium Acetate, pH 5.8/5 % Acetonitrile
• 95 % Water/5 % Acetonitrile
• Mobile Phase
REVERSED PHASE
(C18, C12, C8, C4, C5, C2, C1, Phenyl, PFP, CN, NH2)
Rinse with 10 Column Volumes each of:
• 95 % Water/5 % Acetonitrile (for buffer removal)
• THF
• 95 % Acetonitrile/5 % Water
• Mobile Phase
REVERSED PHASE PROTEIN/PEPTIDE
(C18, C12, C8, C5, C4, Phenyl)
Rinse with 20 column volumes of mobile phase with buffer
removed. Run gradient (2x):
A) 0.1 % TFA in water
B) 0.1 % TFA in Acetonitrile/Isopropanol (1:2)
25 % B to 100 % B for 30 minutes
Equilibrate with 10 column volumes of mobile phase
Do not store column in TFA 
ION EXCHANGE (SAX, SCX, NH2, WAX, WCX)
Rinse with 10 Column Volumes each of:
• 500 mM Phosphate Buffer pH 7
• 10 % Acetic Acid (Aq)
• 5 Column Volumes of Water
• 10 Column Volumes of Phosphate Buffer pH 7
• 5 Column Volumes of Water
• 10 Column Volumes of Methanol
• 10 Column Volumes of Water
For Protein Removal
Follow the above procedure with this exception:
Substitute 10 Column Volumes of Methanol with 10 Column
Volumes of 5 M Urea or 5 M Guanidine Thiocyanate
6
GFC/SEC (BioSep SEC*) *See Part VI for more details
Rinse with 5 column volumes of 0.1 M Phosphate Buffer
pH 3.0. For strongly retained proteins, run the following
gradient: 100 % Water to 100 % Acetonitrile to 100 % Water
over 60 minutes OR wash with 5 column volumes of SDS or 6 M
Guanidine Thiocyanate or 10 % DMSO

Sample Considerations
Always prefilter samples with Phenex™ syringe filters to avoid
particulate contaminants which may clog the column. Use of a
guard column is highly recommended to prolong the life of your
analytical to preparative column.

Part II - SFC (Supercritical

fluid chromatography)
Phenomenex analytical and Axia ‘SFC Approved’ columns have
been leak tested under SFC conditions at pressure far exceeding
what may be expected with normal SFC operation.

Running Parameters SFC

APPROVED
• Backpressure Limitation: 3500 psi
• Flow Rate Limitations: Flow rate is to be controlled so that
pressure limit of 3500 psi is not exceeded
• pH Limitations: Dictated by the media packed in the column

Equilibrating Column
SFC column stationary phases have a polar surface and may
be shipped under reversed phase or normal phase conditions.
Flush all columns with 10-30 column volumes of Methanol/
CO2 as intermediate solvent between CO2 and column shipping
conditions. Be aware of backpressure settings.
Equilibrate column to starting conditions with 10 column volumes
of mobile phase.

Mobile Phase Considerations

• Use only HPLC grade solvent modifiers
• Use only highest purity chemicals and reagents
• Filter and degas all mobile phases prior to use

Cleaning Procedure
• Under extreme conditions the column can be flushed with
50/50 Acetonitrile/Isopropyl Alcohol followed by 100 %
Isopropyl Alcohol. Maintain backpressure below limits.
• Re- Equilibrate column to starting conditions with 10
column volumes of mobile phase

Column Storage
• Completely remove all buffers, acids, bases or other mobile
phase additives to prevent damage to media
• Flush with at least 10 column volumes of Methanol after
the last sample is purified
• Store column with end plugs firmly seated in endfittings to
ensure storage solvent does not evaporate

7
 PART III - AXIA PACKED
PREPARATIVE COLUMN
Running Parameters
• Backpressure Limitation: 3500 psi
• Flow Rate Limitations: Determined by the viscosity
of mobile phase; flow rates to be controlled so that
backpressure limit of 3500 psi is not exceeded
• pH Limitations: Dictated by the media packed in the column

Mobile Phase Considerations

• Use only HPLC grade solvents and water
• Use only highest purity chemicals and reagents
• Filter and degas all mobile phases prior to use

Cleaning Procedure
(for Axia reversed phase columns)
• Reverse flush the column with 10 column volumes of HPLC
grade water and then 10 column volumes of 100 % organic
solvent
• Under extreme conditions, the column can be flushed with
10 column volumes of 100 % THF (or IPA) followed by
100 % methylene chloride
• After cleaning, wash with 100 % THF (or IPA) and 50:50
Acetonitrile/Water, prior to equilibrating with the starting
mobile phase

COLUMN STORAGE
• Completely remove all buffers, acids, bases, or other
mobile phase additives to prevent physical damage to the
media
• Flush with at least 10 column volumes of 50:50
Acetonitrile/Water after the last sample is purified
• Store with column end plugs placed back in the end-fittings
to ensure that the packing media does not dry out
For additional information, consult the Care and Use of Axia Packed
Preparative HPLC Columns, included with each Axia column
purchased.

Part IV - Lux Chiral Columns

Running Parameters
Operating Backpressure
The mobile phase flow rate should be set such that the column
backpressure stays below 300 bar (4300 psi). This maximum
backpressure should not be exceeded for long periods of time.
Operating Temperatures
With standard mobile phases (such as alkane/alcohol) the column
can be used in the temperature range 0-50 °C.

Mobile Phase Considerations

Mobile Phase Compatibility
Lux columns can be used with normal phase (alkane/alcohol),
reversed phase (aqueous methanol, aqueous acetonitrile or
appropriate buffer/methanol or buffer/acetonitrile mixtures), as
well as with pure polar organic solvents (low molecular weight
alcohols, acetonitrile or their mixtures).
Solvent Switching
An appropriate column washing procedure must be applied when
changing from one mobile phase to another. The miscibility
of the different mobile phase components must be carefully
considered for this wash. To safely transfer a column from hexane
8
to methanol (or acetonitrile) or from methanol (or acetonitrile) to
hexane, use 100 % 2-propanol as transition solvent at a flow rate
of 0.2-0.5 mL/min. Ten column volumes of 2-propanol (i.e. 25
mL for a 250 x 4.6 mm ID column or 15 mL for a 150 x 4.6 mm
ID column) are sufficient for completely removing the old mobile
phase. In addition, when the buffer salt additive of the RP mobile
phase is insoluble in 2-propanol, flush the column briefly with
water before switching to a buffered mobile phase.
Switching between elution modes – NP to PO or RP and back to
NP – is possible, but changes in resolution and retention times
may be observed with some compounds. We recommend the
use of dedicated Lux columns to reversed phase operation hence
avoiding the need of converting columns used in normal phase
elution mode to reversed phase or vice versa.
Use of Mobile Phase Modifiers
For basic samples or acidic chiral compounds, it may be necessary
to use an appropriate mobile phase modifier in order to achieve
chiral resolution and to insure proper peak shapes. Diethylamine,
ethanolamine and butyl amine in the concentration range 0.1-
0.5 % can be used with basic analytes, while trifluoroacetic
or acetic acid (0.1-0.5 %; typically 0.1-0.2 %) with acidic
analytes. Mixtures of basic and acidic mobile phase additives
are acceptable (e.g. diethylamine acetate or trifluoroacetate).
Lux columns will deliver consistent results when operated with
mobile phases containing additives at the concentration levels
specified above. However, limited decrease in column efficiency
may occur when a column is used in combination with these
additives. Therefore, we advise to dedicate columns to mobile
phases containing basic additives.
Mobile Phase Restrictions
Lux chiral stationary phases are prepared by coating silica
with various polysaccharide derivatives. Therefore, any
solvent dissolving the polysaccharide derivative (such as
tetrahydrofurane, acetone, chlorinated hydrocarbons, ethylacetate,
dimethylsulfoxide, dimethylformamide, N-methylformamide, etc.)
must be avoided even in trace amounts (e.g. even as sample
solvent).

Extending Lifetime and

Reconditioning
Phenomenex recommends the use of SecurityGuard™ guard
cartridges to extend the lifetime of your column, especially with
samples extracted from complex matrixes. Ideally, samples must
be completely dissolved in the mobile phase or filtered through a
syringe filter of approximately 0.45 µm porosity.

Column Storage
• Column storage for a longer period of time is recommended
in n-hexane/2-propanol (9:1, v/v).
• Columns used in reversed phase conditions should be first
flushed with water (whenever a buffer salt was used as
RP mobile phase additive) and then with methanol (or with
methanol only when no salt was used). The column can be
stored in methanol.

9
 PART V - CHIREX Chiral
Columns
RUNNING PARAMETERS
• Temperature must not exceed 50 °C
• Column pressure must not exceed 3000 psi
• Maintain flow rate between 0.5-2.0 mL/min for 4.6 mm
ID columns

Mobile Phase Considerations

• Dedicate column to reversed or normal phase solvents
• pH range: 2.5 to 7.5
• Use only HPLC grade solvents
• Use only highest purity chemicals and reagents
• Filter and degas all mobile phases prior to use
• Make sure solvents are miscible (see pp. 18-19)
Most CHIREX Chiral columns use a Type I or brush type Chiral stationary
phase (CSP I). Normal phase systems usually provide better selectivity than
reversed phase systems. SEE COLUMN INSERT FOR FURTHER INFORMATION
ABOUT SPECIFIC CHIREX COLUMNS (included with each column)

Sample Considerations
Always prefilter samples with Phenex™ syringe filters to avoid
particulate contaminants which may clog the column. Use of a
guard column is highly recommended to prolong the life of your
analytical to preparative column.

PART VI - BioSep-SEC-S
columns
RUNNING PARAMETERS
• Maximum flow rate is a function of pressure
• Column pressure must not exceed 1500 psi
• Maximum temperature: 50 °C for 316 stainless steel

Mobile Phase Considerations

• pH range: 2.5 - 7.5
• Maximum organic modifier: Up to 100 % CH3CN. Start
with 100 % H2O, linear gradient to 100 % CH3CN over
50 min. Up to 90 % CH3CN, 10 % DMSO or 500 mM
b-mercaptoethanol.
• Maximum salt concentration: 1 M
• Filter and degas all mobile phases prior to use.

Sample Considerations
Always prefilter samples with Phenex™ syringe filters to avoid
particulate contaminants which may clog the column. Use of a
guard column is highly recommended to prolong the life of your
analytical to preparative column.

Cleaning Procedure
• General protein removal: wash with 30 mL of 0.1 M
NaH2PO4, pH 3.0
• Hydrophobic protein removal: use Acetonitrile gradient
• Strongly adsorbed proteins: wash with 30 mL of 0.5 % SDS
or 6 M guanidine thiocyanate, or 10 % DMSO

Column Storage
• Overnight storage: run mobile phase at 0.2 mL/min
• Prolonged storage: use 0.05 % sodium azide in water or
10 % methanol in water

10
 PART VII - REZEX
POLYMER-BASED COLUMN
Running Parameters
• Columns must be run at elevated temperatures
• (60-85 °C) except Rezex ROA and RHM for most
applications
• Column pressure for 8 % cross-linked material must not
exceed 1,000 psi; must not exceed 300 psi for 4 % cross-
linked material
• Clean and reverse flush column regularly with HPLC grade
water
Important: Never exceed maximum pressure limitations. This will
cause irreversible damage to the column.

Mobile Phase Considerations

• Filter and degas all mobile phases prior to use
• Do not exceed 10 % Organic, IPA, EtOH
• Store columns in HPLC grade water
Rezex utilizes a sulfonated polystyrene resin which is very
rugged and resistant to chemical attack. However, the material
is pressure sensitive and must be cared for properly.
START UP
Turn on column heating unit to 60 - 85 °C and start the
mobile phase at 0.1 mL/min. Make sure the pressure
remains below 400 psi for 8 % cross-linked material;
below 200 psi for 4 % cross-linked material. As the temperature
reaches working condition, increase flow rate to the specified
level. (See Rezex Operating Parameters)
SHUT DOWN
Overnight: Lower flow rate to 0.1 mL/min. Leave system on and
continue heating.
Long Term: Store columns in 100 % water. Turn off pump and
allow the system to cool. Replace the end plugs and tightly cap
the column.

Sample Considerations
Always prefilter samples with Phenex™ syringe filters to avoid
particulate contaminants which may clog the column. Use of a
guard column is highly recommended to prolong the life of your
analytical column.

Cleaning Procedure
Before utilizing any cleaning procedure outlined in the
Tables on pages 13 and 15, first try to clean your Rezex column
as follows:
Remove the guard column and reverse the direction of flow on
the analytical column. Run 100 % HPLC grade water through
the column as follows:
Table 7
Rezex Column Flow (mL/min) Temp. (°C)

RPM, RCM, RHM 0.6 85

RCU 0.2 85
RSO and RNO 0.2 75
RNM and RAM 0.4 75
ROA 0.6 85

Run the column under these conditions for a minimum of 12

hours. After completing the cleaning procedure, return the column
to the original direction of flow and equilibrate for analysis.
If this procedure is not effective in cleaning the column, proceed
to the specified procedures outlined in Tables 8 and 9. 11
12
Table 8 RCM Monosaccharide RSO Oligosaccharide RNO Oligosaccharide RNM Carbohydrate RAM Carbohydrate
Part Number 00H-0130-K0 00P-0133-N0 00P-0137-N0 00H-0136-K0 00H-0131-K0
Ionic Form Calcium Silver Sodium Sodium Silver
Standard Dimensions 300 x 7.8 mm 200 x 10 mm 200 x 10 mm 300 x 7.8 mm 300 x 7.8 mm
Matrix Sulfonated Styrene Divinyl Benzene
Cross Linking 8% 4% 4% 8% 8%
Particle Size (µm) 8 12 12 8 8
Min. Efficiency (p/m) based on last peak 35,000 N/A N/A 30,000 35,000
PART VII - REZEX

Typical Pressure (psi @ Max Flow Rate) 400 200 200 400 400
Max. Pressure (psi @ Max Flow Rate) 1,000 300 300 1,000 1,000
Max. Flow Rate (mL/min)* 1.0 0.3 0.3 1.0 1.0
Max. Temperature (°C) 85 85 85 85 85
Typical Mobile Phase Water Water Water Water Water
pH Range Neutral Neutral Neutral Neutral Neutral
Guard Column Part No. 03B-0130-K0 03R-0133-N0 03R-0137-N0 03B-0136-K0 03B-0131-K0
SPECIFICATIONS AND OPERATING PARAMETERS

* Make sure the maximum pressure is not exceeded

See p. 11 for general care and usage of Rezex columns.

POLYMER-BASED COLUMNS (cont’d)
 Table 8 (continued) RCM Monosaccharide RSO Oligosaccharide RNO Oligosaccharide RNM Carbohydrate RAM Carbohydrate
Cleaning, Regeneration and Storage
Organic Modifiers (Max) 10 % Methanol, IPA, EtOH, Acetonitrile
Inorganic Modifiers (Max) 5 % CaS04, Ca(N03)2 , CaCl2 5 % Silver Nitrate 5 % Sodium Salts 5 % Sodium Salts 2 % Silver Nitrate
Avoid Acids, Bases, Non-Calcium Acids, Bases, Non-Silver Acids, Bases, Non-Sodium Acids, Bases, Non-Sodium Acids, Bases, Non-Silver
Salts or Metal Ions, Salts/Metal Ions, Salts/Metal Ions, Salts/Metal Ions, Salts/Metal Ions,
>10 % Organic >5 % Organic >5 % Organic >10 % Organic >10 % Organic
Cleaning Solvent 100 % Water 100 % Water 100 % Water 100 % Water 100 % Water
Flow Rate(mL/min) 0.6 0.2 0.2 0.4 0.4
Temperature (°C) 85 75 75 75 75
Duration (hrs) 12 12 12 12 12
Regeneration Solvent 0.1 M Ca(N03)2 0.1 M AgN03 0.1 M NaN03 0.1 M NaN03 0.1 M AgN03
Flow Rate (mL/min) 0.2 0.1 0.2 0.2 0.2

See p. 11 for general care and usage of Rezex columns.

Temperature (°C) 85 85 85 85 85
Duration (hrs) 4-16 4-16 4-16 4-16 4-16
Ship/Storage Solvent Water Water Water Water Water
and organic acid analysis
COLUMNS FOR carbohYdrate

13
14
Table 9 RPM Monosaccharide RHM Monosaccharide ROA Organic Acid RFQ Fast Acid RCU Sugar Alcohols
Part Number 00H-0135-K0 00H-0132-K0 00H-0138-K0 00D-0223-K0 00G-0130-D0
Ionic Form Lead Hydrogen Hydrogen Hydrogen Calcium
Standard Dimensions 300 x 7.8 mm 300 x 7.8 mm 300 x 7.8 mm 100 x 7.8 mm 250 x 4.0 mm
Matrix Sulfonated Styrene Divinyl Benzene
Cross Linking 8% 8% 8% 8% 8%
Particle Size (µm) 8 8 8 8 8
PART VII - REZEX

Min. Efficiency (p/m) (based on last peak) 35,000 35,000 50,000 (Acetic Acid) 30,000 12,000
Typical Pressure (psi @ Max Flow Rate) 400 400 400 400 400
Max. Pressure (psi @ Max Flow Rate) 1,000 1,000 1,000 1,000 1,000
Max. Flow Rate (mL/min)* 1.0 1.0 1.0 1.0 0.5
Max. Temperature (°C) 85 85 85 85 85
Typical Mobile Phase Water Water 0.005N H2S04 0.005N H2S04 Water
pH Range Neutral 1–8 1–8 1–8 Neutral
Guard Column Part No. 03B-0135-K0 03B-0132-K0 03B-0138-K0 03B-0223-K0 03A-0130-D0
Specifications and OPERATING PARAMETERS

* Make sure the maximum pressure is not exceeded

See p. 11 for general care and usage of Rezex columns.

POLYMER-BASED COLUMNS (cont’d)
 Table 9 (continued) RPM Monosaccharide RHM Monosaccharide ROA Organic Acid RFQ Fast Acid RCU Sugar Alcohols
Cleaning, Regeneration and Storage
Organic Modifiers (Max) 10 % Methanol, IPA, EtOH, Acetonitrile
Inorganic Modifiers (Max) 5 % Lead Nitrate 5 % HN03, H3P04 5 % HN03, H3P04 5 % HN03, H3P04 5 % CaS04, Ca(N03)2, CaCl2
Avoid Acids, Bases, Non-Lead Acids, Bases, Salts, Acids, Bases, Salts, Acids, Bases, Salts, Acids, Bases, Non-Calcium
Salts/Metal Ions, Metal Ions, Metal Ions, pH > 3, Metal Ions, pH > 3, Salts or Metal Ions,
>10 % Organic >10 % Organic >10 % Organic >10 % Organic >10 % Organic
Cleaning Solvent 100 % Water 100 % Water 100 % Water 100 % Water 100 % Water
Flow Rate(mL/min) 0.6 0.6 0.6 0.6 0.2
Temperature (°C) 85 85 85 85 85
Duration (hrs) 12 12 12 12 12
Regeneration Solvent 0.1 M Pb(N03)2 0.025 M H2S04 0.025 M H2S04 0.025 M H2S04 0.1 M Ca (N03)2
Flow Rate (mL/min) 0.2 0.2 0.2 0.2 0.2

See p. 11 for general care and usage of Rezex columns.

Temperature (°C) 85 85 85 85 85
Duration (hrs) 4-16 4-16 4-16 4-16 4-16
Ship/Storage Solvent Water Water 0.005 N H2S04 0.005 N H2S04 Water
and organic acid analysis
COLUMNS FOR carbohydrate

15
 PART VIII - PolySep-GFC-p
columns
RUNNING PARAMETERS
• Column pressure must not exceed 650 psi
• Do not exceed 60 °C

Mobile Phase Considerations

• pH range: 3 - 12
• Maximum salt concentration: 0.5 M
• Organic Modifier capacity:

PolySep Phase
1000 2000 3000 4000 5000 6000 Linear
Methanol 20 % 95 % 70 % 70 % 70 % 70 % 70 %
Acetonitrile 20 % 70 % 70 % 70 % 70 % 70 % 70 %

Cleaning Procedure
0.5 % SDS or 6 M guanidine thiocyanate. All PolySep columns
except for PolySep 1000 may also be cleaned with 50 %
acetonitrile. Make sure not to exceed a maximum pressure of
650 psi when cleaning.

Column Storage
• Overnight storage: run buffer at low flow rate (0.2 mL/min
or less)
• Prolonged storage: store in 0.05 % sodium azide in water
or 10 % methanol in water

Sample Considerations
Always prefilter samples with Phenex™ syringe filters to avoid
particulate contaminants which may clog the column. Use of
a guard column is highly recommended to prolong the life of
your analytical to preparative column.

16
 PART IX - PHENOGEL GPC
columns
Specifications
Matrix: Styrene-Divinyl Benzene Copolymer
Particle Size: 5, 10, 20 µm
Porosities: 50 Å to 106 Å, and mixed beds
Typical Pressure: 5 µm: 300 psi 10 µm: 200 psi
Maximum Pressure: 650 psi
Maximum Temperature: 140 °C (205 °C for UT)
Minimum Efficiency*: 5 µm: 45,000 P/m**
10 µm: 35,000 P/m**
Typical Flow Rates: 4.6 mm ID: 0.35 mL/min
7.8 mm ID: 1.0 mL/min
21.2 mm ID: 7.0 mL/min
End Fittings: Valco Compatible
*Tested in THF ** For 300 x 7.8 mm ID columns

Sample Considerations
Always prefilter samples with Phenex™ syringe filters to avoid
particulate contaminants which may clog the GPC column. Use
of a GUARD COLUMN is highly recommended to prolong the life
of your analytical or preparative column. For optimal results,
use the chart below to determine sample concentrations and
injection volumes.

Table 10
Molecular Weight Concentration (w/v) Max Injection Volume
<50 K 0.5 % 100 µL
50-600 K 0.25 % 100 µL
600-3000 K 0.05 % 100 µL
>3000 K 0.01 % 20 µL

Continued on p. 20

17
18
Polarity Refractive UV (nm) Boiling Viscosity Solubility in
Solvent Miscibility Table Solvent Index Index @ 20 °C Cutoff @ 1 AU Point (°C) (cPoise) Water (% w/w)
Acetic Acid 6.2 1.372 230 118 1.26 100
Table 11
Acetone 5.1 1.359 330 56 0.32 100
Solvent Polarity Chart Acetonitrile 5.8 1.344 190 82 0.37 100
Relative Compound Representative Benzene 2.7 1.501 280 80 0.65 0.18
Polarity Formula Group Solvent Compounds Butyl Acetate 4.0 1.394 254 125 0.73 0.43
NONPOLAR R - H | Alkanes | Petroleum ethers, ligroin, hexanes n-Butanol 3.9 1.399 215 118 2.98 7.81
Ar - H Aromatics Toluene, benzene
R-O-R Ethers Diethyl ether Carbon tetrachloride 1.6 1.466 263 77 0.97 0.08
R-X Alkyl halides Tetrachloromethane, chloroform Chloroform 4.1 1.446 245 61 0.57 0.815
R - COOR Esters Ethyl acetate Cyclohexane 0.2 1.426 200 81 1.00 0.01
R - CO - R Aldehydes Acetone, methyl ethyl ketone 1,2-Dichloroethane1 3.5 1.444 225 84 0.79 0.81
and ketones (MEK) Dichloromethane2 3.1 1.424 235 41 0.44 1.6
R - NH2 Amines Pyridine, triethylamine Dimethylformamide 6.4 1.431 268 155 0.92 100
R - OH Alcohols Methanol, ethanol, Dimethyl sulfoxide3 7.2 1.478 268 189 2,00 100
isopropanol, butanol Dioxane 4.8 1.422 215 101 1.54 100

Increasing Polarity
R - COHN2 Amides Dimethylformamide Ethyl Acetate 4.4 1.372 260 77 0.45 8.7
R - COOH Carboxylic Ethanoic acid Ethanol 5.2 1.360 210 78 1.20 100
acids di-Ethyl Ether 2.8 1.353 220 35 0.32 6.89
POLAR H - OH Water Water Heptane 0.0 1.387 200 98 0.39 0.0003
 Hexane 0.0 1.375 200 69 0.33 0.001
Methanol 5.1 1.329 205 65 0.60 100
Methyl-t-Butyl Ether4 2.5 1.369 210 55 0.27 4.8
Methyl Ethyl Ketone5 4.7 1.379 329 80 0.45 24
Pentane 0.0 1.358 200 36 0.23 0.004
n-Propanol 4.0 1.384 210 97 2.27 100
iso-Propanol6 3.9 1.377 210 82 2.30 100
di-iso-Propyl Ether 2.2 1.368 220 68 0.37
Tetrahydrofuran 4.0 1.407 215 65 0.55 100
Toluene 2.4 1.496 285 111 0.59 0.051
Trichloroethylene 1.0 1.477 273 87 0.57 0.11
Water 9.0 1.333 200 100 1.00 100
Xylene 2.5 1.500 290 139 0.61 0.018

& Miscible
SYNONYM TABLE
Immiscible* 1 4
Ethylene Chloride tert-Butyl Methyl Ether
2 5
Methylene Chloride 2-Butanone
3 6
*Immiscible means that in some proportions two phases will be produced. Methyl Sulfoxide 2-Propanol

Xylene
Water
Trichloroethylene
Toluene
Tetrahydrofuran
di-iso-Propyl Ether
iso-Propanol6
n-Propanol
Pentane
Methyl Ethyl Ketone5
Methyl-t-Butyl Ether4
Methanol
Hexane
Heptane
di-Ethyl Ether
Ethyl Acetate
Ethanol
Dioxane
Dimethyl Sulfoxide3
Dimethylformamide
Dichloromethane2
1,2-Dichloroethane1
Cyclohexane
Chloroform
Carbon tetrachloride
n-Butanol
Butyl Acetate
Benzene
Acetonitrile
Acetone
Acetic Acid

19
 Column Storage
Solvents such as THF (stabilized THF only), Chloroform, Methylene
Chloride, and Toluene are commonly used for column storage.
Be sure to follow solvent switching instructions (see below) if
using solvents other than THF. Storage solvents that remain
liquefied at ambient temperatures and are not oxidizing can be
used for storage.
BE SURE THAT ANY COLUMN THAT IS NOT USED IS CAPPED
TIGHTLY WITH END-PLUGS TO AVOID EVAPORATION OF
SOLVENTS FROM COLUMN. COLUMN DESICCATION IS THE
MOST COMMON SOURCE OF COLUMN FAILURE.

SOLVENT SWITCHING
CONSIDERATIONS FOR
NON-AQUEOUS GPC COLUMNS
Phenogel columns are rugged and exhibit wide solvent
compatibility. Different solvents, however, produce different
swell characteristics (Table 12). Improper solvent switches can
result in a void. For this reason, we recommend that you dedicate
columns to specific solvents.
If you need to switch solvents, it is VERY IMPORTANT to take the
following into consideration:
1. Reduce flow rate to 0.2 mL/min.
2. Backpressure must NEVER exceed 650 psi.
3. Always check solvent miscibility in a beaker or follow the
solvent miscibility table on page 18-19 before proceeding
with ANY solvent switch.
4. Compare the swell characteristics of solvent 1 (old solvent)
to solvent 2 (new solvent) and use the following guideline:
• If the solvent 1 and solvent 2 belong to the same swell
category (Table 12), check the solvent miscibility and
proceed with the switch.
• If solvent 1 and solvent 2 belong to successive swell
categories as indicated by the arrows on Table 12, check
the miscibility and proceed with the switch.
• If solvent 1 and solvent 2 DO NOT belong to the same OR
successive swell categories, switch to an intermediate
solvent FIRST, as indicated by the arrows on Table 12.

Table 12
A – 70-80 % SWELL
Toluene TCB
THF p-Xylene
Chloroform Cyclopentane
Benzene Pyridine
o-Dichlorobenzene

B – 60 % SWELL
Ethyl Ether Cycloheptane
Methylene Chloride Ethyl Acetate
Methyl Ethyl Ketone

C – 50 % SWELL
Acetone Dimethyl Acetamide
m-Cresol N-Methyl Pyrrolidone
o-Chlorophenol DMSO
Dimethyl Formamide Dioxane

D – 30 % SWELL
Acetonitrile Freon 113  n-Butyl Alcohol
Cyclohexane HFIP Methanol
20 n-Hexane Iso-Propanol
 PHENOGEL PORE SIZE Suggested PHENOGEL PORE SIZE Suggested
Linear Operating Linear Operating
Mobile Phase Solvent 50 Å 100 Å 500 Å 103 Å 104 Å 105 Å 106 Å & Mixed Temperature 3 4 5 6 Temperature
Mobile Phase Solvent 50 Å 100 Å 500 Å 10 Å 10 Å 10 Å 10 Å & Mixed
Acetone Y Y Y Y Y Y Y Y Hexane Y Y Y Y Y Y Y Y
Benzene Y Y Y Y Y Y Y Y m-Cresol Y* Y Y Y Y Y Y Y 100 °C
Carbon Tetrachloride Y Y Y Y Y Y Y Y Methyl Ethyl Ketone Y Y Y Y Y Y Y Y
Chloroform Y Y Y Y Y Y Y Y Methylene Chloride Y Y Y Y Y Y Y Y
30 % HFIP/chloroform Y Y Y Y Y Y Y Y o-Chlorophenol Y* Y Y Y Y Y Y Y 100 °C
Diethyl Ether Y Y Y Y Y Y Y Y o-Dichlorobenzene Y* Y Y Y Y Y Y Y 135 °C
Table 13

Dimethylacetamide (DMAC) Y* Y Y Y Y Y Y Y 60 °C Quinolin Y* Y Y Y Y Y Y Y 60 °C

Dimethylformamide (DMF) Y* Y Y Y Y Y Y Y 60 °C Tetrahydrofuran Y Y Y Y Y Y Y Y
Dioxane Y Y Y Y Y Y Y Y Toluene Y Y Y Y Y Y Y Y
DMSO Y* Y Y Y Y Y Y Y 60 °C
Trichlorobenzene Y* Y Y Y Y Y Y Y 135 °C
Ethyl Acetate Y Y Y Y Y Y Y Y
Water N N N N N N N N
Hexafluoroisopropanol (HFIP) Y Y Y Y Y Y Y Y
FOR PHENOGEL GPC COLUMNS

Xylene Y Y Y Y Y Y Y Y
SOLVENT COMPATIBILITY CHART

*Not recommended on 5 µm 50 Å columns. N = Not Compatible Y = Compatible

21
 PART X - Polymerx rp
columns
Specifications
• Matrix: Polystyrene Divinylbenzene (PSDVB)
• Particle Size: 3, 5, 7, 10 µm
• Pore Size: 100 Å

RUNNING PARAMETERS
• Maximum temperature: 60 °C
• Maximum pressure: 2500 psi

Mobile Phase Considerations

• pH range: 0 - 14
• Avoid buffer strength > 0.5 N

Cleaning Procedure
• 100 % Water to 100 % Acetonitrile. Repeat 3 times.

Column Storage
• 75:25 Acetonitrile / Water

PART XI - HPLC COLUMN

PROTECTION & PERFORMANCE
TESTING
• Maximize the life of your valuable HPLC Column
• Reduce system wear and tear
• Save time and money

phenex™
Syringe filters
• Increase column lifetime (save money!)
• Ensure more accurate, consistent results
• Eliminate damaging microparticlates
Particulates can damage expensive equipment, valves, columns
and pumps. They can also lead to erratic analytical results. Pre-
filtering samples prior to analysis is critical in preventing column
and frit blockage, undue wear on valve seals, and abnormally
high operating pressures.
Table 14
Sample or Mobile Filter Membrane
Phase Volume (mL) (diameter, mm) Format
≤2 4 Syringe filter
2 to 10 15-17 Syringe filter
10 to 100 25-28 Syringe filter
> 100 47 Membrane disk
> 1000 90 Membrane disk

Membrane Filters Order list Guide

Regenerated Cellulose (RC)
As a universal hydrophilic membrane, RC is widely used in
chromatography for the clarification of aqueous samples
and solvents. Due to its ultra-low binding capabilities, RC
membranes are an excellent choice for proteins, peptides and
other biomolecules.
Polytetrafluoroethylene (PTFE, Teflon®)
PTFE is an inherently hydrophobic membrane, excellent for
filtration of organic-based, highly acidic or basic samples and
solvents. Widely used in chromatography, it is especially well
suited for the clarification of non-aqueous samples. Although
22 this membrane is hydrophobic, it can be made hydrophilic by
wetting the membrane with alcohol and then flushing with
deionized water.
Polyethersulfone (PES)
Polyethersulfone, a hydrophilic membrane with fast flow, high-
throughput characteristics, with ultra-low protein binding. It
is ideally suited for use in life sciences applications. The PES
membrane offers better chemical resistance than cellulose
acetate. Recommended for filtering critical biological sampling,
tissue culture media, additives, and buffers.
Nylon (NY)
Nylon has inherent hydrophilic characteristics and works well for
filtration of many aqueous and mixed-organic samples. Nylon
exhibits a high non-specific affinity for proteins. Phenomenex
recommends Phenex-RC (Regenerated Cellulose) filters for
application requiring low non-specific adsorption of proteins.
Cellulose Acetate (CA)
Cellulose Acetate membranes exhibit ultra-low protein binding
and are broadly used in the filtration of biological samples.
In combination with a glass pre-filter (Phenex-GF/CA), this
membrane is excellent for filtration of tissue culture media,
general biological sample filtration and clarification.
Glass Fiber (GF)
Glass Fiber filters are made of inert borosilicate glass and have a
nominal 1.2 µm pore size. They are commonly used with highly
viscous samples or samples containing high concentrations of
particulate matter (e.g., food analysis, biological samples, soil
samples, fermentation broth samples, removal of yeasts, molds,
etc.). Glass Fiber filters can be used alone or in conjunction
with other Phenex filter membranes such as the 0.45 µm pore
Phenex-RC filter to reduce clogging of the membrane and
optimize flow.

ORDERING INFORMATION
Part No. Pore Size (µm) Phenex Membrane Housing
4 mm Diameter (500/pk)
AF0-3103-52 0.45 RC PP
AF0-3102-52 0.45 PTFE6 PP
AF3-3107-52 0.45 NY PP
AF0-3203-52 0.20 RC PP
AF0-3202-52 0.20 PTFE6 PP
AF3-3207-52 0.20 NY PP
15–17 mm Diameter (500/pk)
AF0-2103-52 0.45 RC PP
AF0-2102-52 0.45 PTFE6 PP
AF0-2107-52 0.45 NY PP
AF0-2203-52 0.20 RC PP
AF0-2202-52 0.20 PTFE6 PP
AF0-2207-52 0.20 NY PP
25–28 mm Diameter (500/pk)
AF0-8103-525 0.45 RC PP
AF0-8108-527 0.45 PES3 PP
AF0-1102-52 0.45 PTFE6 PP
AF0-1107-52 0.45 NY PP
AF0-8B09-527 0.45 GF/CA2,3,4 MBS
AF0-8203-52 5
0.20 RC PP
AF0-8208-52 7
0.20 PES3 PP
AF0-1202-52 0.20 PTFE6 PP
AF0-1207-52 0.20 NY PP
AF0-8A09-527 0.20 GF/CA2,3,4,7 MBS
AF0-8515-52 7
1.20 GF2,3 MBS
Housing is made of medical-grade polypropylene (PP), unless otherwise indicated. Above
syringe filters are non-sterile.
1. 17 mm diameter. 4. Cellulose acetate is surfactant-free.
2. Glass fiber filters are 28 mm diameter 5. 26 mm diameter.
and made of borosilicate. They will 6. Hydrophobic membrane. Can be made
remove 90 % of all particles >1.2 µm. hydrophilic by pre-wetting with IPA.
3. Housing material is methacrylate 7. 28 mm diameter.
butadiene styrene (MBS) polymerisate.
Also known as Cryolite™.

23
 PHENEX™ DISPOSABLE CENTRIFUGAL
FILTER UNITS
• Convenient filtration of multiple HPLC and GC samples
• High recovery for small samples
• Nylon, Cellulose Acetate, and PTFE (Teflon®)
membrane materials

Centrifugal force drives the sample through the filter quickly

without effort on the part of the chemist. No cleaning of syringes
is required between samples. The receiver tube serves as a
container for the filtered sample and can be retained as long
as desired.

ORDERING INFORMATION
Pore Volumes (mL) Membrane
Part No. Size (μm) Sample/Receiver Non-Sterile Unit
AF0-0438 0.2 2.0 / 5.0 Nylon 25/pk
AF0-0439 0.45 2.0 / 5.0 Nylon 25/pk
AF0-0440 0.2 2.0 / 5.0 PTFE 25/pk
AF0-0441 0.45 2.0 / 5.0 PTFE 25/pk
AF0-8353 0.2 2.0 / 5.0 CA 25/pk
AF0-8354 0.45 2.0 / 5.0 CA 25/pk
Above centrifugal filters are non-sterile.

24
Guard Cartridge
System

SecurityGuard provides a great balance of convenience, column

protection capability and value. If you’ve ever used another
guard cartridge system or conventional guard column, you will
be pleasantly surprised when you see how practical and effective
SecurityGuard really is. This highly advanced, patented design
offers several unique features up to now not available.

Clean Dirty
CONVENIENCE
Knowing when to replace your guard is no longer a mystery!
SecurityGuard’s direct-view feature lets you inspect the packing
material for visual contaminants and indicates when it’s time
to replace the cartridge. No other guard cartridge has this
convenient feature.

EXTRA PROTECTION
SecurityGuard offers the option of stacking two cartridges in
the same holder, using the simple stacking ring provided. Extra
length provides extra protection. When the first cartridge becomes
exhausted, contaminants are retained by the second cartridge.

VERSATILITY
One direct-connect holder conveniently finger-tightens into
virtually any brand of HPLC column worldwide. How can one
holder be direct-connect and universal at the same time when
end-fittings have different depths? Answer- the length of the
stainless steel nib at the end of the holder automatically adjusts
to the precise depth of a column’s endfitting. SecurityGuard’s
fingertight connection will withstand pressures up to 5000 psi and
it features a completely inert and biocompatible flowpath.

ACCURACY
The cartridges can be used with virtually any matching phase of
virtually any brand of column without affecting efficiency, retention
time or backpressure. There are 34 different phases to choose
from, including cartridges for general purpose, pharmaceutical,
protein and polypeptide, aqueous size exclusion, carbohydrate
and organic acid applications. SecurityGuard phases can be
used with columns containing 3, 3.5, 4, 5, 10, 15 µm or larger
diameter particle sizes.

Continued on p. 26

25
 Security Guard
ORDERING INFORMATION
Analytical Holder Assembly Kit
Part No. Description Unit
KJ0-4282 Guard Cartridge Kit ea

Kit includes: 1 Cartridge Holder, 3 PEEK Ferrules, 2 Stacking Rings,

2 PEEK Fingertight Male Nuts, 2 Wrenches

Semi-Preparative and Preparative Holder for

10.0, 21.2 and 30.0 mm ID cartridges
Part No. Description Unit
AJ0-7220 Holder for 10.0 mm ID cartridges ea
AJ0-8223 Holder for 21.2 mm ID cartridges ea
AJO-8277 Holder for 30.0mm ID cartridges ea

Semi-Preparative Preparative

If your HPLC column Internal Diameter, ID (mm) is:

2.0 - 3.0 3.2 - 8.0 9.0 - 16.0 18.0 - 29.0 30.0 - 49.0
Analytical SemiPrep PREP

If your HPLC column Internal Diameter, ID (mm) is:

4.0 - 2.0 4.0 - 3.0 10 - 10.0 15 - 21.2 15 - 30.0

Cartridges - General Purpose / Pharmaceutical

pH Dimensions
Part No. Material Description Stability L x ID(mm) Unit
AJ0-4286 C18 (ODS, Octadecyl) 1.5-10 4 x 2.0 10/pk
AJ0-4287 C18 (ODS, Octadecyl) 1.5-10 4 x 3.0 10/pk
AJ0-7221 C18 (ODS, Octadecyl) 1.5-10 10 x 10 3/pk
AJ0-7839 C18 (ODS, Octadecyl) 1.5-10 15 x 21.2 ea
AJ0-8301 C18 (ODS, Octadecyl) 1.5-10 15 x 30 ea
AJ0-6073 C12 (Dodecyl) 1.5-10 4 x 2.0 10/pk
AJ0-6074 C12 (Dodecyl) 1.5-10 4 x 3.0 10/pk
AJ0-7275 C12 (Dodecyl) 1.5-10 10 x 10 3/pk
AJ0-7842 C12 (Dodecyl) 1.5-10 15 x 21.2 ea
AJ0-8304 C12 (Dodecyl) 1.5-10 15 x 30 ea
AJ0-4289 C8 (Octyl, MOS) 1.5-10 4 x 2.0 10/pk
AJ0-4290 C8 (Octyl, MOS) 1.5-10 4 x 3.0 10/pk
AJ0-7222 C8 (Octyl, MOS) 1.5-10 10 x 10 3/pk
AJ0-7840 C8 (Octyl, MOS) 1.5-10 15 x 21.2 ea
AJ0-8302 C8 (Octyl, MOS) 1.5-10 15 x 30 ea
AJ0-4292 C5 (Pentyl) 1.5-10 4 x 2.0 10/pk
AJ0-4293 C5 (Pentyl) 1.5-10 4 x 3.0 10/pk
AJ0-7372 C5 (Pentyl) 1.5-10 10 x 10 3/pk
AJ0-4298 C1 (TMS) 2-9 4 x 2.0 10/pk
AJ0-4299 C1 (TMS) 2-9 4 x 3.0 10/pk
AJ0-7373 C1 (TMS) 2-9 10 x 10 3/pk
AJ0-4347 Silica — 4 x 2.0 10/pk
AJ0-4348 Silica — 4 x 3.0 10/pk
AJ0-7223 Silica — 10 x 10 3/pk
AJ0-7229 Silica — 15 x 21.2 ea
AJ0-8312 Silica — 15 x 30 ea
AJ0-8328 HILIC 1.5-8 4 x 2.0 10/pk
AJ0-8329 HILIC 1.5-8 4 x 3.0 10/pk
AJ0-4301 NH2 (Amino, Aminopropyl) 4 x 2.0 10/pk
AJ0-4302 NH2 (Amino, Aminopropyl) 4 x 3.0 10/pk
AJ0-7364 NH2 (Amino, Aminopropyl) 10 x 10 3/pk
AJ0-8162 NH2 (Amino, Aminopropyl) 15 x 21.2 ea
AJ0-8309 NH2 (Amino, Aminopropyl) 15 x 30 ea

26 (Continued on next page)

Cartridges - General Purpose / Pharmaceutical
(Continued)
pH Dimensions
Part No. Material Description Stability L x ID(mm) Unit
AJ0-4304 CN (Cyano, Cyanopropyl) 4 x 2.0 10/pk
AJ0-4305 CN (Cyano, Cyanopropyl) 4 x 3.0 10/pk
AJ0-7313 CN (Cyano, Cyanopropyl) 10 x 10 3/pk
AJ0-8220 CN (Cyano, Cyanopropyl) 15 x 21.2 ea
AJ0-8311 CN (Cyano, Cyanopropyl) 15 x 30 ea
AJ0-4350 Phenyl (Phenylhexyl) 1.5-10 4 x 2.0 10/pk
AJ0-4351 Phenyl (Phenylhexyl) 1.5-10 4 x 3.0 10/pk
AJ0-7314 Phenyl (Phenylhexyl) 1.5-10 10 x 10 3/pk
AJ0-7841 Phenyl (Phenylhexyl) 1.5-10 15 x 21.2 ea
AJ0-8303 Phenyl (Phenylhexyl) 1.5-10 15 x 30 ea
AJ0-8326 PFP(2) (Pentafluorophenylpropyl) 4 x 2.0 10/pk
AJ0-8327 PFP(2) (Pentafluorophenylpropyl) 4 x 3.0 10/pk
AJ0-8376 PFP(2) (Pentafluorophenylpropyl) 10 x 10 3/pk
AJ0-8377 PFP(2) (Pentafluorophenylpropyl) 15 x 21.2 ea
AJ0-8378 PFP(2) (Pentafluorophenylpropyl) 15 x 30 ea
AJ0-4307 SCX (SA, Strong Cation Exchanger) 4 x 2.0 10/pk
AJ0-4308 SCX (SA, Strong Cation Exchanger) 4 x 3.0 10/pk
AJ0-7369 SCX (SA, Strong Cation Exchanger) 10 x 10 3/pk
AJ0-4310 SAX (SA, Strong Cation Exchanger) 4 x 2.0 10/pk
AJ0-4311 SAX (SA, Strong Cation Exchanger) 4 x 3.0 10/pk
AJ0-7370 SAX (SA, Strong Cation Exchanger) 10 x 10 3/pk
AJ0-5808 RP-1(Reversed Phase Polymer) 4 x 2.0 10/pk
AJ0-5809 RP-1(Reversed Phase Polymer) 4 x 3.0 10/pk
AJ0-7368 RP-1(Reversed Phase Polymer) 10 x 10 3/pk
AJ0-8358 RP-1(Reversed Phase Polymer) 15 x 21.2 ea
AJ0-6075 Polar-RP (Ether-linked Phenyl) 4 x 2.0 10/pk
AJ0-6076 Polar-RP (Ether-linked Phenyl) 4 x 3.0 10/pk
AJ0-7276 Polar-RP (Ether-linked Phenyl) 10 x 10 3/pk
AJ0-7845 Polar-RP (Ether-linked Phenyl) 15 x 21.2 ea
AJ0-8307 Polar-RP (Ether-linked Phenyl) 15 x 30 ea
AJ0-7556 Fusion-RP (C18 Polar Embedded) 4 x 2.0 10/pk
AJ0-7557 Fusion-RP (C18 Polar Embedded) 4 x 3.0 10/pk
AJ0-7558 Fusion-RP (C18 Polar Embedded) 10 x 10 3/pk
AJ0-7844 Fusion-RP (C18 Polar Embedded) 15 x 21.2 ea
AJ0-8306 Fusion-RP (C18 Polar Embedded) 15 x 30 ea
AJ0-7510 AQ C18 (Polar Endcapped C18) 4 x 2.0 10/pk
AJ0-7511 AQ C18 (Polar Endcapped C18) 4 x 3.0 10/pk
AJ0-7512 AQ C18 (Polar Endcapped C18) 10 x 10 3/pk
AJ0-7843 AQ C18 (Polar Endcapped C18) 15 x 21.2 ea
AJ0-8305 AQ C18 (Polar Endcapped C18) 15 x 30 ea
AJ0-7596 Gemini C18 (TWIN Technology) 4 x 2.0 10/pk
AJ0-7597 Gemini C18 (TWIN Technology) 4 x 3.0 10/pk
AJ0-7598 Gemini C18 (TWIN Technology) 10 x 10 3/pk
AJ0-7846 Gemini C18 (TWIN Technology) 15 x 21.2 ea
AJ0-8308 Gemini C18 (TWIN Technology) 15 x 30 ea
AJ0-8367 Gemini-NX (C18 TWIN-NX Technology) 4 x 2.0 10/pk
AJ0-8368 Gemini-NX (C18 TWIN-NX Technology) 4 x 3.0 10/pk
AJ0-8369 Gemini-NX (C18 TWIN-NX Technology) 10 x 10 3/pk
AJ0-8370 Gemini-NX (C18 TWIN-NX Technology) 15 x 21.2 ea
AJ0-8371 Gemini-NX (C18 TWIN-NX Technology) 15 x 30 ea
AJ0-7914 Gemini C6-Phenyl (TWIN Tech.) 4 x 2.0 10/pk
AJ0-7915 Gemini C6-Phenyl (TWIN Tech.) 4 x 3.0 10/pk
AJ0-8134 Oligo-RP (C18 TWIN Technology) 4 x 2.0 10/pk
AJ0-8135 Oligo-RP (C18 TWIN Technology) 4 x 3.0 10/pk
AJ0-8136 Oligo-RP (C18 TWIN Technology) 10 x 10 3/pk
AJ0-8210 Oligo-RP (C18 TWIN Technology) 15 x 21.2 ea
AJ0-8310 Oligo-RP (C18 TWIN Technology) 15 x 30 ea
AJ0-8324 Oligo-WAX 4 x 3.0 10/pk
(WA, Weak Anion Exchanger)
AJ0-8325 Oligo-WAX 10 x 10 3/pk
(WA, Weak Anion Exchanger)

AJ0-8420 Oligo-WAX 15 x 30 ea
(WA, Weak Anion Exchanger)
27
 Security Guard ORDERING
INFORMATION (continued)
Cartridges for Protein/Polypeptide Reversed Phase
For use with all silica columns for separation of proteins and peptides, such as Jupiter®
(Phenomenex); Vydac® 218TP, 214TP (Alltech Associates, Inc.); SynChropak® 300 C18,
C4 (Eprogen, Inc.); Nucleosil® 300 Å C18, C4; HYPERSIL® 300 Å and all other widepore
or 300 Å brands.
pH Dimensions
Part No. Material Description Stability L x ID(mm) Unit
AJ0-4320 Widepore C18 (ODS) 1.5-10 4 x 2.0 10/pk
AJ0-4321 Widepore C18 (ODS) 1.5-10 4 x 3.0 10/pk
AJ0-7224 Widepore C18 (ODS) 1.5-10 10 x 10 3/pk
AJ0-7230 Widepore C18 (ODS) 1.5-10 15 x 21.2 ea
AJ0-8313 Widepore C18 (ODS) 1.5-10 15 x 30 ea
AJ0-4326 Widepore C5 (Pentyl) 1.5-10 4 x 2.0 10/pk
AJ0-4327 Widepore C5 (Pentyl) 1.5-10 4 x 3.0 10/pk
AJ0-7371 Widepore C5 (Pentyl) 1.5-10 10 x 10 ea
AJ0-4329 Widepore C4 (Butyl) 1.5-10 4 x 2.0 10/pk
AJ0-4330 Widepore C4 (Butyl) 1.5-10 4 x 3.0 10/pk
AJ0-7225 Widepore C4 (Butyl) 1.5-10 10 x 10 3/pk
AJ0-7231 Widepore C4 (Butyl) 1.5-10 15 x 21.2 ea
AJ0-8314 Widepore C4 (Butyl) 1.5-10 15 x 30 ea

Cartridges for Silica GFC (Aqueous SEC)

For use with all sillica GFC columns, such as BioSep™ (Phenomenex); ZORBAX®
GF-series; Bio-Sil®(Bio-Rad)
Dimensions
Part No. Material Description pH Stability L x ID(mm) Unit
AJ0-4487 GFC-2000 2-7.5 4 x 3.0 10/pk
AJ0-7365 GFC-2000 2-7.5 10 x 10 3/pk
AJ0-8588 GFC-2000 2-7.5 15 x 21.2 ea
AJ0-4488 GFC-3000 2-7.5 4 x 3.0 10/pk
AJ0-7366 GFC-3000 2-7.5 10 x 10 3/pk
AJ0-8589 GFC-3000 2-7.5 15 x 21.2 ea
AJ0-4489 GFC-4000 2-7.5 4 x 3.0 10/pk
AJ0-7367 GFC-4000 2-7.5 10 x 10 3/pk
AJ0-8590 GFC-4000 2-7.5 15 x 21.2 ea

Cartridges for Chiral

For use with chiral columns, such as Lux™ Cellulose-1, -2, -3, -4, & Amylose-2
(Phenomenex); CHIRALCEL® OD-H ®, CHIRALCEL® OJ-H ®, & CHIRALPAK ® AD ®-H (DAICEL
Chemical Industries Ltd.)
Dimensions
Part No. Material Description* pH Stability L x ID(mm) Unit
AJ0-8402 Lux Cellulose-1 2-9 4 x 2.0 10/pk
AJ0-8403 Lux Cellulose-1 2-9 4 x 3.0 10/pk
AJ0-8404 Lux Cellulose-1 2-9 10 x 10 3/pk
AJ0-8405 Lux Cellulose-1 2-9 15 x 21.2 ea
AJ0-8406 Lux Cellulose-1 2-9 15 x 30 ea
AJ0-8398 Lux Cellulose-2 2-9 4 x 2.0 10/pk
AJ0-8366 Lux Cellulose-2 2-9 4 x 3.0 10/pk
AJ0-8399 Lux Cellulose-2 2-9 10 x 10 3/pk
AJ0-8400 Lux Cellulose-2 2-9 15 x 21.2 ea
AJ0-8401 Lux Cellulose-2 2-9 15 x 30 ea
AJ0-8471 Lux Amylose-2 2-9 4 x 2.0 10/pk
AJ0-8470 Lux Amylose-2 2-9 4 x 3.0 10/pk
AJ0-8472 Lux Amylose-2 2-9 10 x 10 3/pk

(Continued on next page)

28
 Cartridges for Chiral (cont’d)
Dimensions
Part No. Material Description* pH Stability L x ID(mm) Unit
AJ0-8473 Lux Amylose-2 2-9 15 x 21.2 ea
AJ0-8474 Lux Amylose-2 2-9 15 x 30 ea
AJ0-8621 Lux Cellulose-3 2-9 4 x 2.0 10/pk
AJ0-8622 Lux Cellulose-3 2-9 4 x 3.0 10/pk
AJ0-8623 Lux Cellulose-3 2-9 10 x 10.0 3/pk
AJ0-8624 Lux Cellulose-3 2-9 15 x 21.2 ea
AJ0-8625 Lux Cellulose-3 2-9 15 x 30.0 ea
AJ0-8626 Lux Cellulose-4 2-9 4 x 2.0 10/pk
AJ0-8627 Lux Cellulose-4 2-9 4 x 3.0 10/pk
AJ0-8628 Lux Cellulose-4 2-9 10 x 10.0 3/pk
AJ0-8629 Lux Cellulose-4 2-9 15 x 21.2 ea
AJ0-8630 Lux Cellulose-4 2-9 15 x 30.0 ea
* Lux Cellulose-1 is cellulose tris(3,5-dimethylphenylcarbamate)
Lux Cellulose-2 is cellulose tris(3-chloro-4-methylphenylcarbamate)
Lux Amylose-2 is amylose tris(5-chloro-2-methylphenylcarbamate)
Lux Cellulose-3 is cellulose tris(4-methylbenzoate)
Lux Cellulose-4 is cellulose tris(4-chloro-3-methylphenylcarbamate)

Cartridges for Carbohydrate / Organic Acid

For Organic acid and carbohydrate analysis, such as Rezex™ (Phenomenex); Aminex®
(Bio-Rad); Interaction; Sugar-Pak™ (Waters).
Dimensions
Part No. Material Description pH Stability L x ID(mm) Unit
AJ0-4490 Carbo-H +
1-8 4 x 3.0 10/pk
AJ0-4491 Carbo-Ag+* Neutral 4 x 3.0 10/pk
AJ0-4492 Carbo-Pb+2 Neutral 4 x 3.0 10/pk
AJ0-4493 Carbo-Ca+2 Neutral 4 x 3.0 10/pk
*For use with saccharide and oligosaccharide columns in Ag+ form.

Replacement Parts
Part No. Description Unit
AJ0-4283 PEEK Ferrules 3/pk
AJ0-4285 Stacking Rings 2/pk
AQ0-1389 PEEK Fingertight Male Nuts 10/pk
AJ0-4284 Security Guard Wrenches 2/pk
AQ0-8374 PREP Coupler, SS w/ PEEK Ferrule Inserts ea
10-32 Threads, 1/16 in. OD x 0.020 in. ID
AQ0-8375 Replacement Ferrule Inserts, for PREP 10/pk
Coupler, PEEK, 0.020 in. ID
AQ0-8222 PREP Replacement O-Rings, Kalrez® 2/pk
For 15 x 21.2 mm SG Holder, Size 2-021
AQ0-8318 PREP Replacement O-Rings, Kalrez® 2/pk
For 15 x 30 mm SG Holder, Size 2-025

29
 HPLC SYSTEM TEST KIT

• Diagnose hardware problems rapidly and easily

• Avoid unnecessary and costly system repairs
• Convenient benchmark testing of HPLC systems
using a C18 column standard
• Test system setup and hardware connections
• Quickly isolate method development problems
• Reduce instrument down time

Each kit contains

the following:
1. Phenomenex 5 µm C18,
50 x 4.6 mm HPLC column
2. Five vials of Isocratic Test Mix
3. Five vials of Gradient Test Mix

ORDERING INFORMATION
Part No. Description Unit
CH0-1684 | HPLC System Test Kit, Reversed | ea
Phase, includes: C18 column,
isocratic and gradient test mixes
CH0-1685 Isocratic Test Mix 5/pk
CH0-1686 Gradient Test Mix 5/pk

COLUMN PERFORMANCE
CHECK STANDARDS
• Convenient way to check column performance
• Affordable and easy to use

Phenomenex offers a comprehensive line of column performance

check standards to help you evaluate column performance. We
recommend using the check standards to verify performance of all
columns upon receiving them and periodically over the lifetime of
the column. Test conditions are located in the column jacket.

Normal PHASE Part No. AL0-3033

(For Si, NH2, NO2, Alumina, PAC, and Luna CN)
Unit quantity: 2 mL
Contains: Meta-xylene, Nitrobenzene

REVERSED PHASE 1 Part No. AL0-3034

(For C1, C18, CN and Phenyl)
Unit quantity: 2 mL
Contains: Uracil, Benzamide, Benzophenone, Biphenyl

REVERSED PHASE 2 Part No. AL0-3045

(For Prodigy C8, ODS(2), ODS(3); Luna C5, C8, C18, Phenyl-
Hexyl, PFP(2); Jupiter C4, C5, C18, Proteo; Columbus C8,
C18; Aqua; Synergi; PhenoSphere-NEXT C8, C18; Gemini
C18, C6-Phenyl; Gemini-NX C18; Clarity Oligo-RP; Kinetex
C18, PFP)
Unit quantity: 2 mL
Contains: Uracil, Acetophenone, Toluene, Naphthalene
(Please refer to the QC Test Data for specific test conditions for Jupiter and
Luna)

30
COLUMN PERFORMANCE CHECK
STANDARDS (continued)

HILIC PHASE Part No. AL0-8317

(For Luna HILIC; Kinetex HILIC)
Unit quantity: 2 mL
Contains: Toluene, Uracil, Cytosine

CARBOHYDRATE MIX 1 Part No. AL0-3035

(For Rezex RNM, RAM and other carbohydrate analysis
columns)
Unit quantity: 2 mL
Contains: Maltotriose Hydrate, Maltose, Ribitol

CARBOHYDRATE MIX 2 Part No. AL0-3036

(For Rezex RPM and other carbohydrate analysis columns)
Unit quantity: 2 mL
Contains: Melezitose, Glucose, Fructose, Ribitol

CARBOHYDRATE MIX 3 Part No. AL0-3037

(For Rezex RCM, RCU, and other carbohydrate analysis
columns)
Unit quantity: 2 mL
Contains: Melezitose, Maltose, Glucose, Mannose,
Fructose, Ribitol

OLIGOSACCHARIDE STANDARD Part No. AL0-3038

(For Rezex RSO, RNO, and other oligosaccharide analysis
columns)
Unit quantity: 2 mL
Contains: Light corn syrup

ORGANIC ACID STANDARD Part No. AL0-3039

(For Rezex ROA and other organic acid analysis columns)
Unit quantity: 2 mL
Contains: Oxalic acid, Succinic acid, Citric acid,
Formic acid, Tartaric acid, Acetic acid

CATION-EXCHANGE Part No. AL0-3040

(For SCX, SA, CM)
Unit quantity: 2 mL
Contains: Uracil, Cytosine

ANION-EXCHANGE Part No. AL0-3041

(For SAX, SB, DEAE, PEI)
Unit quantity: 2 mL
Contains: Uridine, UMP

31
 COLUMN PERFORMANCE CHECK
STANDARDS (continued)

Aqueous SEC 1 Part No. AL0-3042

(For BioSep-SEC-S and other protein SEC columns)
Unit quantity: Dry; Reconstituted to 2 mL
Contains: Bovine thyroglobulin
Human gamma globulin
Ovalbumin
Myoglobin
Uridine
(reconstitute with 1 mL of 100 mM Sodium Phosphate pH=6.8

AQUEOUS SEC 2 Part No. AL0-3043

(For PolySep GFC-P and other aqueous-soluble analysis
columns)
Unit quantity: 2 mL
Contains: Ethylene Glycol

STAR-ION A300 Part No. AL0-3420

Unit quantity: 2 mL
Contains: Conc. (mg/mL)
Fluoride 5 Nitrite 20
Nitrate 20 Sulfate 20
Chloride 10 Bromide 20
Phosphate 30

POLYMERX RP-1 Part No. AL0-7260

Unit quantity: 2 mL
Contains: Conc. (mg/mL)
Cytosine 13
Uracil 13
Uridine 33

Onyx monolithic reversed

phase Part No. AL0-7836
Unit quantity: 2 mL
Contains: Conc. (µg/mL)
Thiourea 10
Progesterone 100
Anthracene 10

Onyx monolithic NORMAL

phase Part No. AL0-7835
Unit quantity: 2 mL
Contains: Conc. (µg/mL)
Toluene 21.75
Nitrobenzene 150
2-Nitroanisol 0.18

32
COLUMN PERFORMANCE CHECK
STANDARDS (continued)

Chiral Test Mix No. 1 Part No. AL0-3046

Applicable to the following Chirex columns:
3001, 3005
Unit quantity: 2 mL
Contains: 1. S-(+)-2,2,2-trifluoro-1-(9-anthryl)
ethanol CAS [60646-30-2]
2. R-(-)-2,2,2-trifluoro-1-(9-anthryl)
ethanol CAS [53531-34-3]

Chiral Test Mix No. 2 Part No. AL0-3047

Applicable to the following Chirex columns:
3010, 3011, 3012
Unit quantity: 2 mL
Contains: N-dansyl-DL-valine
(cyclohexylammonium salt)
CAS[84540-67-0]

Chiral Test Mix No. 3 Part No. AL0-3048

Applicable to the following Chirex columns:
3014, 3017, 3018, 3019, 3020, 3022
Unit quantity: 2 mL
Contains: 1. (R)-(-)-N-(3,5-Dinitrobenzoyl)- -
methylbenzylamine CAS [69632-32-2]
2. (S)-(-)-N-(3,5-Dinitrobenzoyl)-
methylbenzylamine CAS[69632-31-1]

Chiral Test Mix No. 4 Part No. AL0-3049

Applicable to the following Chirex column:
3126
Unit quantity: 2 mL
Contains: DL-Aspartic Acid CAS [617-45-8]

Chiral Test Mix No. 5 Part No. AL0-8412

Applicable to the following Lux columns:
Lux Cellulose -1,-2,-3,-4, Lux Amylose-2
Unit quantity: 2 mL
Contains: Trans-Stilbene oxide CAS [1439-07-2]

33
 PART XII - SOLID PHASE
EXTRACTION (SPE)
Increase column and instrument life by injecting samples
cleaned-up with Strata®.

STRATA™-X Polymeric Sorbents

Tubes and 96-Well Plates
• Deconditioning Resistant
• Low Elution Volumes
• High Analyte Capacity

Strata™-X for simplified cleanup

of polar and non-polar compounds
Strata™-X-C for selective extraction of basic compounds
Strata™-X-CW for bases (including quaternary amines)
Strata™-X-A for cleanup of weak acids
Strata™-X-AW for acids

STRATA® Traditional Sorbents

Tubes and 96-Well Plates

• Optimal Flow
• Lot-to-Lot Reproducibility
• Wide Range of Selectivity
• Available chemistries include:
C18-E, C18-U, C18-T, C8,
Phenyl, SDB-L, CN, Si-1, WCX, FI-PR, NH2, SAX, SCX,
Melamine

STRATA® Flash Sorbents

• Polar & Non-polar Phases
• Narrow Particle Range Distribution
• Can be used for Direct Scale-up

Strata® Giga™ Tubes available in

12, 20, 60 & 150 mL formats
Sepra™ Bulk available in gram to multi-kilogram quantities

STRATA® On-line Cartridges

• Rapid Extraction and Concentration FLOW
• Direct Inject Analysis
• Easily Automated
Strata™-X for polar and non-polar compounds
Strata™-X-C for weak bases
Strata™-X-CW for strong bases
Strata® C18 for non-polar compounds
Strata® C8 for compounds of intermediate polarity
Aqua, Gemini, Jupiter, Luna, and Strata are registered trademarks
of Phenomenex, Inc. Axia, BioSep, Columbus, Giga, Kinetex,
Lux, Onyx, Phenex, Phenogel, PhenoSphere, PolymerX, PolySep,
Prodigy, Rezex, SecurityGuard, Sepra, Star-Ion, Strata-X, Synergi,
ThermaSphere, TWIN-NX and TWIN Technology are trademarks
of Phenomenex, Inc. Alltima is a trademark of Alltech Associates,
Inc. Bio-Sil and Aminex are registered trademarks of Bio-Rad
Laboratories. Chirex is a registered trademark of Chirex, Inc. licensed
to Phenomenex. Cyrolite is a trademark of CY/RO Industries.
Hypersil is a registered trademark of Thermo Hypersil-Keystone.
Nucleosil is a registered trademark of Macherey-Nagel. Sugar-Pak
is a trademark of Waters Corporation. Symmetry is a registered
trademark of Waters Corporation. SynChropak is a registered
trademark of Eprogen, Inc. Teflon is a registered trademark of E.I.
du Pont de Nemours and Co. Vydac is a registered trademark of
Alltech Associates, Inc. Zorbax is a registered trademark of Agilent
Technologies. © 2010 Phenomenex. All rights reserved. Onyx is a
product based on monolicthic technology under license from Merck
KGaA, Darmstadt, Germany.
Subject to Phenomenex Standard Terms & Conditions which may
be viewed at www.phenomenex.com/TermsAndConditions.
34
PART XIII - HPLC ACCESSORIES
Accessories
• Backpressure Regulators
• Biocompatible / Metal-free products
• Connectors and Splitters
• Filtration Products
• Injectors and Injector Loops
• Membrane Filters
• Mobile Phase Handling Devices
• Polymer Calibration Standards / Kits
• Rotor Seals, Stators, etc.
• Solvent Reservoir and Reagent Bottles
• SPE Consumables, Tube & Plate Manifolds
• Switching Valves
• Syringes
• Syringe Filters
• Tools
• Tubing, Fittings, Frits and Unions
• Valves (Injection, Switching)
• Vials, Caps and Septa

equipment
• Column Chiller-Heater
• Column Heater
• Column Selectors
• Degasser
• Fluid Processors
• Mobile Phase Recycler
• Temperature Controllers
For ordering and additional information, please contact your Phenomenex
Technical Consultant.

SINGLE COLUMN HEATER THERMASPHERE™ TS-130

• Compact, low-cost heater precisely controls temperature
from 25-90 °C
• Improves reproducibility and chromatographic results
• Reduces analyte identification errors
• Improves baseline and overall detector performance
• Improves peak efficiency and analyte quantitation
(especially at low levels)
• Improves the ruggedness of the
separation (within-lab
and lab-to-lab)

ORDERING INFORMATION
ThermaSphere™ TS-130
Part No. Description
EH0-7057 ThermaSphere TS-130 HPLC Column Heater 25-90 °C,
95 to 265 VAC, 50/60 Hz
EH0-7058 Stand for ThermaSphere TS-130 HPLC Column Heater
More Accessories available. See Phenomenex Catalog for details.

35
 PHENOMENEX WARRANTY
Phenomenex products are warranted to meet the stated
performance and quality and to be free of defects in
material and workmanship. They are not warranted, nor
does Phenomenex assume liability, if misused. NO OTHER
WARRANTY OR REPRESENTATION IS IMPLIED OR EXPRESSED
BY PHENOMENEX FOR ITS PRODUCTS WITH RESPECT TO
MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE,
OR ANY OTHER MATTER. PHENOMENEX SHALL NOT UNDER
ANY CIRCUMSTANCES BE LIABLE FOR ANY INCIDENTAL,
CONSEQUENTIAL, OR COMPENSATORY DAMAGE ARISING
FROM THE USE OF, OR IN CONJUNCTION WITH, ITS PRODUCTS.
The maximum liability which can be assumed by Phenomenex for
breach of warranty shall be the invoice price of the product.

Specific Warranties on HPLC

Columns
Phenomenex warrants its quality columns in accordance with the
following terms and conditions. Phenomenex will repack, replace,
or refund charges on any column (at our discretion), at no cost if
a column fails to perform satisfactorily. Columns being returned
must have prior return authorization granted by Phenomenex.
Defective products must be accompanied by a written explanation
of failure. Approval is subject to the following exclusions:
• All columns must be tested upon receipt and all
deficiencies
must be reported to Phenomenex no later than 15 days
after the date of receipt of the column.

• Maximum warranty period is limited to 90 days on HPLC

columns unless previously agreed upon. However,
COLUMNS MAY NOT BE RETURNED FOR REFUND OR
CREDIT AFTER 45 DAYS AND WITHOUT PRIOR
AUTHORIZATION.

• Removal of column end-fittings automatically voids column

warranty.

• Column performance warranty is limited to the conditions

of the original test chromatograms.

• Physical damage to the column due to misuse, abuse, or

mishap, including mechanical shock.

• Chemical damage to the packing material due to operation at

incorrect chemical conditions, temperatures, or pressures.

• Failure due to high backpressures caused by improper

solvent or sample filtration practices causing particulate
build-up or precipitation in the column or end-fitting.

• Incorrect selection of packing material made by customer for

their particular use or incompatibility of equipment, etc.

• For products supplied by but not manufactured by

Phenomenex, the warranty is limited by the terms of the
original manufacturer’s warranty.

36
 column protection guide

COLUMN PERFORMANCE RECORD

Column: ____________________________________

Dimensions: _________________________________

Serial No.: __________________________________

Dates Sample/ No. Back- Storage

User
Used Method Injections Pressure Solvent

37
 www.phenomenex.com
Phenomenex products are available worldwide. For the distributor in your country,
contact Phenomenex USA, International Department by telephone, fax or email: international@phenomenex.com.
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mail: PO Box 4084 Zeppelinstr. 5 411 Madrid Ave. Gydevang 39-41 Parc des Grillons, Bat.3 Zeppelinstr. 5
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fax: 02-9428-6445 01-319-1300 (310) 328-7768 4810 6265 01 30 09 21 11 06021-58830-11
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