NF1200/NF1200R

MULTI – PURPOSE BENCH TOP

CENTRIFUGE
USER’S MANUAL

NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş.

Z14.K25.249 REV.NO.:11 REV.DATE:01/2018
MANUFACTURER

NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş.

Saracalar Mah. Saracalar Kümeevleri No: 4/2

Akyurt 06750 Ankara

TEL: +(90) 312 399 28 30 (pbx)

FAX: +(90) 312 399 21 97

E-mail:satis@nuve.com.tr

WARRANTY CERTIFICATE

1. Nüve warrants that the equipment delivered is free from defects during material and
workmanship. This warranty is provided for a period of two years. The warranty period
begins from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided with
the equipment.
3. Nüve does not accept any liability in the case where the goods are not used in
accordance with their proper intent.
4. The warranty may not be claimed for damages occurred during the shipment, for
damages resulting from improper handling or use, the defects in maintenance,
negligence, bad functioning of auxiliary equipment, in the case of force majeure or
accident and incorrect power supply.
5. In the event of failure, Nüve shall be under no liability for any injury, or any loss or
damage as the result of the failure other than the guarantee conditions.
• BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE READ
CAREFULLY.
• THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF
THE INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS MANUAL.
• INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF NÜVE. IT
MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT PERMISSION.

1
DEAR NUVE USER

We would like to take this opportunity to thank you for preferring this Nüve product. Please
read the operating instructions carefully and keep them handy for future reference.

Please detain the packing material until you see that the unit is in good condition and it is
operating properly. If an external or internal damage is observed, contact the transportation
company immediately and report the damage. According to ICC regulations, this
responsibility belongs to the customer.

While you are operating the instrument please;

• obey all the warning labels,

• do not remove the warning labels,
• do not operate damaged instrument,
• do not operate the instrument with a damaged cable,
• do not move the instrument during operation.

In case of a problem contact your Nüve agent for an authorized service or maintenance.

The validity of the guarantee is subject to compliance with the instructions and precautions
described in this manual.

Nüve reserves the right to improve or change the design of its products without any
obligation to modify previously manufactured products.

Information contained in this document is the property of Nüve. It may not be duplicated or
distributed without its permission.

PLEASE REGISTER ONLINE TO VALIDATE WARRANTY:

To register your warranty online, please visit our web page www.nuve.com.tr and fill in WARRANTY
REGISTRATION FORM.

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TABLE OF CONTENTS
SECTION 1 – INTRODUCTION ............................................................................................ 4
1.1. USE AND FUNCTION ............................................................................................. 4
SECTION 2 – TECHNICAL SPECIFICATIONS ..................................................................... 5
2.1. TECHNICAL SPECIFICATIONS TABLE ................................................................. 5
2.2. ACCESSORIES FOR NF 1200/1200R .................................................................... 6
2.3. GENERAL PRESENTATION .................................................................................. 7
2.4. ROTOR SELECTION TABLE .................................................................................. 8
2.5. PRECAUTIONS AND USAGE LIMITATIONS ......................................................... 9
SECTION 3 – SYMBOLS .....................................................................................................10
SECTION 4 – INSTALLATION .............................................................................................10
4.1. LIFTING AND TRANSPORT ..................................................................................10
4.2. UNPACKING ..........................................................................................................10
4.3. POSITIONING........................................................................................................11
4.4. MAIN SUPPLY .......................................................................................................11
SECTION 5 – OPERATING UNIT ........................................................................................11
5.1. OPERATING ..........................................................................................................11
5.2. DISPLAY AND CONTROL PANEL.........................................................................12
5.3. MAKING A NEW PROGRAM .................................................................................14
5.3.1 SELECTING THE MEMORIZED PROGRAM .....................................................15
5.3.2 PULSE MODE ....................................................................................................16
5.4 END OF OPERATION............................................................................................17
5.5 SAFETY INTERLOCK SYSTEM ............................................................................17
5.6 IMBALANCE DETECTION SYSTEM .....................................................................17
5.7 MANUAL LID OPENING ........................................................................................18
SECTION 6 – OPERATING PRINCIPLES............................................................................18
6.1 PREPARATION OF THE ROTOR TO RUN ...........................................................18
6.2 LOADING ...............................................................................................................18
6.3 ROTOR INSTALLATION ........................................................................................19
6.4 DRIVE SYSTEM ....................................................................................................20
SECTION 7 – CLEANING AND PERIODIC MAINTENANCE ...............................................20
7.1 PERIODIC MAINTENANCE ...................................................................................20
7.2 STERILIZATION ....................................................................................................21
7.3 CORROSION INFORMATION ...............................................................................21
7.3.1 CHEMICAL CORROSION ..................................................................................21
7.3.2 STRESS CORROSION ......................................................................................21
7.4 CLEANING .............................................................................................................22
7.5 ELECTRICITY ........................................................................................................22
SECTION 8 – DISPOSAL MANAGEMENT CONCEPT ........................................................22
BÖLÜM 9 – TROUBLESHOOTING ......................................................................................23
SECTION 10 – ELECTRICAL CIRCUIT DIAGRAMS ...........................................................24
SECTION 11 – WARNING LABEL .......................................................................................26

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SECTION 1 – INTRODUCTION

1.1. USE AND FUNCTION

The NF 1200 / NF 1200 R centrifuges are designed to separate liquids of different densities
by applying centrifugal force. Different types of rotors, which are swing-out and angle rotors,
can be fitted to the centrifuges.

The microprocessor control system of the centrifuges enables the user to observe and to
control the speed (RPM or RCF), the acceleration and braking rates, run time, program
number and temperature of the bowl for NF 1200 R. The microprocessor control system also
displays the RPM or RCF value during the run.

The ventilation system of the NF 1200 limits temperature in the bowl between the ambient
temperature + 3 °C and ambient temperature + 12 °C depending on the rotor type. The NF
1200 R is equipped with a cooling system which provides to operate between -9 °C and + 40
° C.

It ensures safe processes by means of the locking system which does not allow the
centrifuge to operate if the lid is open and which does not allow the rotor to spin if the lid is
not closed.

The lid locking system which does not allow the centrifuge to operate if the lid is open, which
also does not allow the lid to be opened while the rotor is spinning and the possibility to open
the lid by pushing only one key when the program ends, provide safe and easy working
conditions. There are audible and visible alarms to inform the operator when the lid is open,
when the program ends and when any error conditions occur. In case of a power failure, the
lid could be opened manually by using a manual lid opening tool

The NF series centrifuges are manufactured according to the following standards,

EN 61010-1, EN 61010-2-020, EN 61000-6-3, EN 50419, EN 61326-1.

This device is in compliance with WEEE Regulation.

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SECTION 2 – TECHNICAL SPECIFICATIONS

2.1. TECHNICAL SPECIFICATIO NS TABLE

NF 1200 NF 1200 R
RA 280 rotor: 4100 rpm, MP 200 rotor: 4100 rpm,
RS 600 rotor: 9000 rpm, RS 100 rotor: 12000 rpm, RS24 rotor: 14000
Max Speed
rpm,
RS 30 rotor: 13500 rpm
RA 280 rotor: 3082 x g, MP 200 rotor: 2349 x g, RS 600 rotor: 8965 x g,
Max. RCF
RS 100 rotor: 14167, RS24 rotor : 18405 x g, RS 30 rotor: 20170 x g
RA 280 rotor: 4x280 ml, MP 200 rotor: 2x3 microtitre plates,
Max. Capacity RS 600 rotor: 6x100 ml, RS 100 rotor: 10x10 ml, RS 24 rotor: 24x1.5/2
ml, RS 30 rotor: 30x2 ml
Control System Microprocessor controlled
Display High Visibility Digital
No. of Memories 10
Speed Range 500-14000 rpm
Speed Step 10 rpm
Speed Accuracy ± 20 rpm
Timer Range 01-99 min + Hold Position
Timer Step 1 min
Acceleration Rate 0: Slowest 9: Fastest
Braking Rate 0: Slowest 9: Fastest
Power supply 230 V, 50 Hz
Temp. range -9° C / 40 ° C
Refrigerant Liquid R134a
Temp. Accuracy 1°C
Temp. (at maximum speed) ambient
+4°C at max. speed
Performance temp+12°C
Max. Power 500 W 800 W
Ext. Dimension 455 x 545 x 405 770 x 545 x 410
Packing Dim. 600 x 530 x 470 870 x 650 x 550
Net / Gross
42 / 47 kg 75 / 80 kg
Weight

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2.2. ACCESSORIES FOR NF 1 200/1200R

A 08 062 Puller for rotor removal

B 50 016 RA 280 swing- out rotor 4*280 ml , 4.100rpm , 3.082 x g
G 51 007 Set of 4 buckes for RA 280
G 51 036 Set of 4 buckets with sealing cap
G 51 008 Set of 4 insert 1x280 ml
G 51 009 Set of 4 insert 1x250 ml flat
G 51 010 Set of 4 inserts 1x200 ml flat
G 51 011 Set of 4 inserts 1x100 ml
G 51 012 Set of 4 inserts 2x50 ml conical
G 51 013 Set of 4 inserts 4x25 ml
G 51 014 Set of 4 inserts 2x15 ml conical
G 51 015 Set of 4 inserts 12x15 ml
G 51 016 Set of 4 inserts 12x5/7 ml
G 51 017 Set of 4 inserts 19x5 ml RIA
B 50 021 MP 200 microtitre rotor 4.100 rpm 2.349 xg
B 50 023 RS 600 Angle rotor 6x100 ml 9.000 rpm , 8.965 xg
G 51 018 Set of 6 adaptors 1x50 ml
G 51 019 Set of 6 adaptors 1x50 ml cocinal
G 51 020 Set of 6 adaptors 1x30/38 ml
G 51 021 Set of 6 adaptors 1x15 ml cocinal
B 50 022 RS 100 Angle rotor 10x10 ml 12.000 rpm ,14.167xg
G 51 022 Set of 6 adaptors 1x6 ml
G 51 023 Set of 6 adaptors 1x1.5/2 ml
B 50 014 RS 24 Angle rotor 24 x1,5/2 ml 14.000 rpm , 18.407xg
A 14 005 Adaptor 500/800 µl
A 14 006 Adaptor 200 µl PCR
A 14 007 Adaptor 250/400/700 µl

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2.3. GENERAL PRESENTATION

Figure 1

Figure 2

1 LID 6 Manual LİD opening hole

2 Lock pin 7 Control panel
3 Gas spring 8 Bowl
Electrıcal cables inlet, On / off switch and
4 Lid Gasket 9
fuses
5 Rotor 10 Cooling unit (Only NF1200R)

7
2.4. ROTOR SELECTION TABLE

Swing – Out Rotor And Accessories;

Max. Max.RCF
Max.Tube Radius xg
Rotor Description Capacity Speed
Dia.(mm) (mm)
(rpm)
Swing-out Rotor 4x280ml 164 4,100 3,082
380 ml
Set of 4 buckets 76
nominal
Set of 4 inserts 1x280ml 65
Set of 4 inserts 1x250ml flat 62
Set of 4 inserts 1x200ml flat 55
RA280 Set of 4 inserts 1x100ml 39
2x50ml
Set of 4 inserts 30
conical
Set of 4 inserts 4x25ml 25
5x15ml
Set of 4 inserts 17
conical
Set of 4 inserts 12x15ml 17
Set of 4 inserts 12x5/7ml 13.5
Set of 4 inserts 19x5ml RIA 13
2x3
Microtitre Plate
MP200 microtitre 125 4,100 2,349
Rotor
plates

8
Angle Rotor Accessories;

Max.
Max.Tube Max.Speed
Rotor Description Capacity Radius Max RCFxg
Dia(mm) (rpm)
(mm)
Angle Rotor 6x100ml 38 99 9,000 8,965
Set of 6
1x50ml 30
adaptors
RS600 Set of 6 1x50 ml
30.5
adaptors conical
Set of 6
1x30/38ml 26
adaptors
Set of 6 1x15 ml
17
adaptors conical
Angle Rotor 10x10ml 16 88 12,000 14,167
RS100 Set of 10
1x6ml 13
adaptors
Set of 10
1x1.5/2ml 11
adaptors
Angle Rotor 24x1.5/2ml 11 84 14,000 18,405
Adaptor 1x500/800µl 8
RS24 1x200 µl
Adaptor 6.5
PCR
1x250/400/70
Adaptor 6
0 µl
Angle Rotor 30x2ml 11 99 13.500 20.170
Adaptor 1x500/800µl 8
RS30 1x200 µl
Adaptor 6.5
PCR
1x250/400/70
Adaptor 6
0 µl

2.5. PRECAUTIONS AND USAG E LIMITATIONS

• Do not use the device for any purpose other than the usage purpose.
• Prior to first use, the user’s manual should be read and the device is only to be used by
authorized and trained personnel. Only authorized technical personnel handle the
product in case of any failure.
• The working bench should be durable to the device weight and vibration isolated.
• Ensure that the rotor is placed correctly prior to usage.
• According to the standard IEC 61010-2-020, anyone and any hazardous materials
should not be in the 300 mm safety zone while centrifuge is running.
• Do not move the device while it is running.
• Do not open the lid while rotor is spinning.
• Apply the manual lid opening procedure in the case of power cut or in the case of any
error.
• Use only the the spare parts, rotors and accessories which are supplied by NUVE.
• Load the rotor according to the explanations in the user’s manual.
• Start the device after ensuring the rotor is loaded correctly.
• Do not use the centrifuge in areas which are in explosive danger.

9
• Do not centrifuge the explosive, flammable, radioactive, corrosive materials and the
materials which may react with each other.
• The centrifuge and the rotor are not microbiologically leak-proof. Use tubes with leak-
proof covers, if hazardous, toxic and pathogenic microorganisms are centrifuged.
• Do not use corrosive materials which may be harmful for the device integrity, rotor and
accessories.
• Do not use rotors and accessories with corrosion and mechanical damages.
• Mains supply should be appropriate to power of the device and grounded.
• Use tubes whose sizes are suitable to the rotor and accessories.
• Tubes which are used in the centrifuge should not be deformed by the effect of the
centrifuge force.
• Use glass tubes to balance, if glass tubes are used. Use plastics tubes to balance, if
plastics tubes are used.
• Do not start the device unless tubes are in balance.
• Imbalance loading may cause mixing the samples, broken tubes, and damages on the
rotor and motor shaft.

If mentioned warnings are not considered, nüve will not be responsible

from their results.

SECTION 3 – SYMBOLS

Symbol in the operating instructions: Attention, general hazard area.

This symbol refers to safety relevant warnings and indicates possibly
dangerous situations. The non-adherence to these warnings can lead to
material damage and injury to personal.

Symbol in the operating instructions: This symbol refers to important

circumstances.

SECTION 4 – INSTALLATION

4.1. LIFTING AND TRANSPORT

Because of the heavy weight of the centrifuge, all lifting and transport must be carried out
using proper handling equipment. The centrifuge should be lifted from underneath and never
be turned over.

4.2. UNPACKING

Remove the packing cardboard box and the second nylon packing around the centrifuge.
The below written are provided with the instrument, please check them;
• 1 ea. user’s manual
• 1ea. Warranty certification
• 1 ea. power cable
• 1 ea. manual lid opening tool
• 1 ea. 24 socket wrench
• 1 ea. Wrench
• Lubricant oil

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4.3. POSITIONING

• Check that no damage has occurred during transport.

• Check that the positioning is suitable for users.
• Lift the centrifuge (use proper handling equipment if necessary) underneath and carry it
to its place. Check that the centrifuge is stable on its four pads.
• The bench-top must be rigid enough to support the weight and vibrations.
• Leave 30 cm. free space on the every side of the centrifuge.
• Open the lid by using unlocking tool and check no substances left in the bowl. (See the
manual lid opening section 5.7 )
• Make sure that the centrifuge does not occupy the utilization space of nearby
equipment or do damage to them.
• The user should be able to follow the centrifuge during the operation.

According to the standard IEC 61010-2-020, anyone and any

hazardous materials should not be in the 300 mm safety zone while
centrifuge is running.

The centrifuges are designed to operate safely under the following

conditions:
- Indoor use only
- Ambient temperature: 5 ° C to 25 ° C.
- Maximum relative humidity of 80% for temperature up to 22 °C.
- Maximum altitude: 2000 m.
- Temperature for maximum performance: 15 °C / 25 °C.

4.4. MAIN SUPPLY

The centrifuge requires 230 V, 50 / 60 Hz. Please make sure that the supplied mains match
the required power ratings. If no, provide an extra line to support.

Always plug the sterilizer/ovens to properly earthed sockets.

A supply fitted with a circuit breaker should be used for protection

against indirect contact in case of an insulation fault.

SECTION 5 – OPERATING UNIT

5.1. OPERATING

• Open the lid and check that there is no sample in the instrument.
• Turn the centrifuges on by using On/Off switch.
• See that the display and control panel activates.
• Learn the functions of the control panel (See Part 5.2).
• Set the values and start the operation (See Part 5.3)

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5.2. DISPLAY AND CONTROL PANEL

Figure 3 - Control Display Panel

01- Status Display

Display of speed (500-14.000 rpm) or display of RCF (0-18.405 x g) during the run, rotor type
during programming and error code.

02- Time Display

Display of the run time from 1 min. to 99 min. and hold position (H).

03- Acceleration/Brake/Temperature Display

Display of the acceleration and brake rates from 0 to 10, for NF 1200 R it also displays the
bowl temperature during programming and the run.

04- Program No Display

Display of the program number from 0 to 9.

05- Running Indicator Leds

The 4 leds light up consecutively during the run to indicate the spinning of the rotor.

06- ENCODER Button

This has two functional move. Turn clockwise and opposite clockwise, reach program menu,
set value and “READY” situation. By turning the Encoder button increase or decrease values
during programming stage. When Led is “READY” situation, by pushing Encoder button start
device. Stops the run by starting the braking phase manually.

07-LID Key

Opens the lid if the lid indicator lights up.

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08- LID Indicator Led

It turns on if the lid remains open or is not properly closed.

09- Imbalance Indicator Led

It is activated if imbalance occurs. Brake is applied immediately.

10- Acceleration Indicator Led

It turns on while the acceleration rate is being programmed and while the rotor is
accelerating.

11- Braking Indicator Led

This indicator turns on while the breaking rate is being programmed and while the rotor is
breaking.

12- Cooling Indicator Led*

It indicates that the cooling system functions. During programming, it indicates that the
temperature value is being set on Acc/Br/Temp Display (3).

* It is not valid for NF 1200 model.

13- PULSE Button

PULSE mode on if pushing button by during READY situation. It is pushed to see the RPM or
RCF value on the speed display during the run, to pass among the displays and to store the
programmed values.

14- Program Number Led

It indicates that the user is on the program menu.

15- Set Menu Led

It indicates that the user is on the set value menu.

16- READY Led

It indicates that the device ready to run.

13
5.3. MAKING A NEW PROGRAM

Determine the rotor type, the speed and time, the acceleration and braking rates.

14
 ROTOR TYPE DISLPAY MAX. (RPM) MAX. (RCF)
RA 280 Swing – Out 4.280 4,100 3,082
MP 200 Swing – Out PLAtE 4,100 2,349
RS 600 Angle 6.100 9,000 8,965
RS 100 Angle 10.10 12,000 14,167
RS 24 Angle 24. 1.5 14,000 18,406
RS 30 Angle 30. 1.5 13500 19968

Figure 4 - Angle Rotor (left), Swing-out Rotor (right)

The correct rotor type should be selected in order to see the correct
RCF values during the run.

Pre Cooling;
While operating the NF 1200 R centrifuge at sub-ambient
temperatures, in order to get the maximum performance, without
loading the rotor run the centrifuge at 1.500 rpm, at a temperature
which is 3° C lower than the required temperature and at hold position
until you see that the temperature inside the bowl drops to the required
temperature. Then, stop the centrifuge, load your samples and start
centrifugation.

5.3.1 SELECTING THE MEMORIZED PROGRAM

Select the requested program number at stand-by position by turning Encoder button (6)
value increase and decrease. All values related to the selected program will be shown on the
relevant displays. Check that all values are correct.

• Load the rotor with samples by paying attention to the dynamic and static balances.

15
• Close the lid, see that the lid open warning led (8) turns off.
• See that the READY led (16) turns on and push Encoder button (6).
• The rotor starts accelerating according to the set value and the acceleration led turns
on. The set speed or RCF value is displayed during the set time duration. (RPM or
RCF screen will appear in every 3 seconds). For the operations whose speeds are
higher than 2.000 rpm, the rotor accelerates according to the set acceleration value up
to 2.000 rpm and then continues accelerating with the highest acceleration value, 9 to
reach the set speed. As the speed reaches the set value, “-“ sign appears on the
acc/brk/temp display (3).
• The elapsed time is counted down as the centrifugation starts.
• When the time display shows “00”, the program ends, the breaking led turns on and the
set break rate is shown at acc/brk/temp display (3). The rotor brakes with the highest
break rate, 9 until it slows down to 2.000 rpm and continues braking according to the
set braking value.
• The elapsed time until the rotor stops is counted and is shown at the time display. This
is the time that passes until the rotor stops.
• When the speed display shows “0”, the “end” expression appears. The user is warned
by an intermittently sounding alarm.
• Push the LID button (7) to open the lid.
• You may leave the centrifuge at stand-by position.

5.3.2 PULSE MODE

• Load the rotor with samples by paying attention to the dynamic and static balances.
• Set the all parameters for program (See 5.3)
• Close the lid, see that the lid open warning led (8) turns off.
• See that the READY led (16) turns on and push Encoder button (6).

• You can reach the desired speed by pressing and holding the Encoder button (6) within
the RPM speed limits you have set.
• In the PULSE mode, the rotor operates according to the set acceleration and braking
values you set.
• While operating in Pulse mode, press the Encoder button (6) and start counting from
the moment the rotor starts to accelerate. When you take your hand out of the Encoder
button, it will stop counting by braking.

You may display the RPM or RCF value on the speed display during
the run by pushing the function button.

16
 At hold position, the rotor keeps spinning until the Encoder button (6) is
pushed. After the Encoder button has been pushed, the rotor brakes
according to the set brake rate.

The centrifuge waits at the stand-by position with the program values of
the last program.

Please open the lid after every operation, otherwise the centrifuge
cannot be run again.

5.4 END OF OPERATION

At the end of the cooling operation with the NF 1200 R centrifuges, dry with a non-wool soft
cloth or paper towel at the centrifuge cell. (When the end of cooling operation with NF1200,
has a puddle at the centrifuge cell. So dry to water with paper towel and soft cloth.)

5.5 SAFETY INTERLOCK SYSTEM

The safety interlock system prevents opening of the lid when the rotor is spinning.

The centrifuge does not operate until the lid is closed and the lid remains locked until the
rotor stops spinning. The “Lid Open” indicator turns on to warn the user if the lid is not closed
properly.

If power failure occurs, access to the samples is possible by opening

the lid with a special tool. Please see the manual lid opening section
(3.6.) for further information.

5.6 IMBALANCE DETECTION SYSTEM

The imbalance detection system of the NF 1200 / NF 1200 R centrifuges operates

electronically when an unacceptable imbalance occurs. In this case the brake is applied
immediately.

The “Err 1” imbalance error is displayed and the imbalance indicator (9) turns on. The lid
cannot be opened until the rotor stops spinning. The centrifuge can only be started after the
lid has been opened and the rotor has been re-loaded correctly.

To avoid facing imbalance problem, please make sure to insert the tubes correctly.

17
5.7 MANUAL LID OPENING

In case of power cut or any breakdown, the centrifuge can be opened manually to access the
samples.

To open the lid manually:

• Switch off the instrument.

• Insert the manual lid opening tool (allen key) into the hole on the front side of the
instrument (see the general view drawing).
• Push the tool keeping it horizontal until the lid opens.
• Turn left or right side the tool, and open the lid.
• After opening the lid manually, turn off and on device.

Before opening the lid manually, make sure that the rotor already
stopped spinning. Upon opening the lid, lift it by hand and observe the
rotor. If the rotor is still spinning, close the lid and wait approximately
10 minutes before repeating the operation. This operation must be
carried by someone who is informed of the danger and of the
precautions which must be undertaken.

After opening the lid manually, turn off and on device.

SECTION 6 – OPERATING PRINCIPLES

6.1 PREPARATION OF THE ROTOR TO RUN

Before installation, check the rotor for corrosion and cleanliness.

Chemical corrosion or mechanical corrosion may do severe damage to the rotor and the
centrifuge. Particles which are stuck inside the inserts cause the breakage of tubes and lead
to major imbalance please check to make sure that no particles are left on the rotor.

The central hole of the rotor and the motor shaft should also be clean and dry before all
centrifugal operations and they must be kept in that way all the time.

6.2 LOADING

The most important condition of an efficient centrifugation is to balance the tubes properly.
Loading must be done by meeting the requisites of static and dynamic balance.

Static Balance: This balance states that diametrically oppositely replaced weights are
almost the same. In application, the liquid level in the tubes should be at the same height to
balance the load.

Dynamic Balance: This balance states that diametrically oppositely replaced center of the
gravity of tubes are symmetrical with respect to spinning axis of the rotor.

• Although in hospitals the samples having almost the same densities are centrifuged, in
industry, samples having different densities may be centrifuged. In this case, the
dynamic balance becomes more important factor than the static balance is.

18
• If the number of tubes to be centrifuged is less than the capacity of the rotor, the tubes
must be placed oppositely. If an odd number of tubes is centrifuged, a water filled tube
at the same weight should be used for balancing.

Imbalance of the rotor may cause major damage to the rotor and
centrifuge.

Never attempt to introduce liquids into the tube inserts.

Balance the rotor with glass tubes if you use glass tubes for
centrifugation.
Balance the rotor with plastic tubes if you use plastic tubes for
centrifugation.

Always use tubes which can withstand to the set speeds.

Examples of the proper and improper loading are shown below.

Figure 5

6.3 ROTOR INSTALLATION

• Put some light oil on the drive head to prevent sticking.

• Be careful that the rotor fits on the drive shaft (See Figure 6).
• Screw the shaft nut with socket wrench to the clockwise direction. Make sure that shaft
nut is screwed tightly, but do not expose over-force to the socket wrench while
screwing.
• For swing-out rotors, lubricate the pins where the buckets are hanged.
• Place the buckets to the rotor.
• Connect the centrifuge to the power supply.

19
 Figure 6

6.4 DRIVE SYSTEM

• The rotor is driven by a three phase asynchronous motor. The microprocessor control
system assures the correct drive speed.
• The force applied to the rotor is directly related to the shape of the rotor, the swing-out
rotor receives more load than the angle rotor does. Longer radius and more
accessories increase the load of the rotor and decrease the spinning speed.
• The centrifuge does not allow the rotor to spin at a speed which it cannot resist
mechanically.
• Please make that sure the correct type of rotor is selected during programming.
SECTION 7 – CLEANING AND PERIODIC MAINTENANCE

7.1 PERIODIC MAINTENANCE

• Disconnect the power cable and after the maintenance check the presence of the
mains grounding line.
• Rotors should be washed after every use, especially if a spillage has occurred, in warm
water containing a few drops of liquid soap. (A mild washing liquid is ideal as a
cleaner).
• Rotors and other accessorıes must be clean if any spillage or chemicals occur.
• You may use a nylon brush to clean the buckets and tube inserts of the rotor.
• Do not use metal brushes.
• Dry the rotor with a piece of soft absorber cloth. Please make sure that the buckets and
inserts are well dried, you may use hair dryer.
• The buckets of the swing-out rotors should be greased frequently with the oil provided
with the centrifuge. Please remove the light oil from the pins and put a small amount of
fresh oil every time you grease. This will ensure free swinging of the buckets. Most of
the imbalance problems are mostly raised by the users who do not clean and oil the
pins.
• Please do not leave the rotor on a metal surface, particularly stainless steel as
electrochemical reactions set off easily with the aluminum or magnesium in the rotor.
• Make sure that no deposit remains at the bottom of the bucket because the pressure of
a flask or tube from above during centrifugation will certainly increase the chance of
corrosion.

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7.2 STERILIZATION

• Apply alcohol, for example %70 ethanol or isoprophanol, for 10 minutes against
bacteria and viruses.
• The rotors and buckets may be autoclaved at 121°C and under 215 kPa pressure for
20 minutes but please do not forget to remove all accessories.
• Do not use formaldehyde for the sterilization process.
• Phenol is a corrosive substance and should never be used.
• Glutaraldehyde is a toxic substance and increases the rate of fatty acid in the body.
7.3 CORROSION INFORMATIO N

• Nuve rotors which are made of aluminum are designed to spin at proper RCFs for
many years. When used properly, their resistance to corrosion and their life span
increases and the imbalance problems decrease.
• All accessories should be checked thoroughly and regularly as almost all laboratories
already have the conditions which lead to corrosion easily.

7.3.1 CHEMICAL CORROSION

This type of corrosion is caused by chemical reactions. The electrolide liquid on the surface
of the material is the main cause of the chemical reaction. If that electrolide liquid is allowed
to stay at the surface, corrosion occurs. First, discoloration appears and then the metal
pittens. Aluminum easily reacts with the ionic solutions.
The other causes of corrosion are as follows,

• Chemical vapors in the laboratory environment which dissolve in the water on the rotor
(in refrigerated centrifuges)
• Corrosive liquids which overflow from overfilled and unsealed tubes. (the liquids which
spread out during centrifugation)
• Contaminated and non-cleaned buckets, tubes and bottles.

If the centrifuged samples are corrosive, only rinsing with water is not
sufficient. The residuals dissolve in the water and humidity on the rotor
and in the buckets.

Some particles may stick to the tubes, buckets and adapters. These
particles crash and do harm to anodized surface during centrifugation
and ease the occurrence of corrosion.

7.3.2 STRESS CORROSION

This type of corrosion is caused by the force of the centrifugation of the corrosive chemical
which is already in contact with the alloy. As the aluminum alloy contacts with the corrosive
chemical, the stress corrosion starts. This type of corrosion is even more dangerous than the
chemical corrosion as the effects of this corrosion are microscopic and very difficult to
observe in the course of time.

The corrosive material is pushed against the aluminum alloy by the centrifugation “g” force
during the centrifugation. This situation causes the stress corrosion to occur more quickly

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than the chemical corrosion does. Microscopic cracks occur under the force of the
centrifugation.

Every centrifugation causes the aluminum rotor to be attacked by the chemical more and
more and eventually micro-cracks decrease the resistance of the rotor against the
centrifugation force. Fortunately, no crash occurs just after the first micro-cracks have
appeared as the rotors are manufactured according to the high safety limits.

The corrosion of the small amount of corrosive materials does not result in severe cracks but
weakens the mechanical resistance of the rotor in the course of time.

7.4 CLEANING

• Disconnect the centrifuge before cleaning.

• There is no need of daily cleaning unless a tube breakage occurs or any liquid spills.
7.5 ELECTRICITY

Centrifuged at high voltages are present behind the panels. These panels are electrically
disconnecting Do not open the centrifuge.
SECTION 8 – DISPOSAL MANAGEMENT CONCEPT

The currently valid local regulations governing disposal must be observed. It is in the
responsibility of the user to arrange proper disposal of the individual components.

Applicable local regulations for disposal have to be carefully observed.

The instruments and electronic accessories (without batteries, power packs etc.) must be
disposed off according to the regulations for the disposal of electronic components.

Batteries, power packs and similar power source have to be dismounted from
electric/electronic parts and disposed off in accordance with applicable local regulations.

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BÖLÜM 9 – TROUBLESHOOTING

If the centrifuge fails to operate,

Check that,

• The on/off switch is on,

• The fuses are sound,
• The plug is not defective,
• The centrifuge is well connected to the supply,
• The electricity installation is not defective,
• Power is supplied.
In case of below written failures, related error codes are shown.

Err 1
• Imbalance error. Wrong loading.
• To avoid facing imbalance problem, please make sure to insert the tubes correctly. (See 5.6)
Err 2

• Motor overheat. Please switch off the centrifuge for at least 20 minutes
Err 3

• Speed failure. The optical sensor could have failed. Please observe whether the
“running” indicator leds are on or not. If it is not on, the optical sensor is defective. If it is
on, the motor could have failed. Contact to your service if either occurs.
• This error code occurs when the mains voltage drops below the lower limit.
Err 4

• Lid failure. This failure occurs when lid is opened during the centrifugation.
Err 5

• The temperature sensor endings are broken. This error code appears only on NF 1200R.
Err 6
• Motor failure. The motor driver unit (inverter) is defective. Switch off the centrifuge and
switch on again after the motor has stopped. If you still see the error code, contact to the
service.
Err 7
• “Lid Open” switch are broken on the Lid Board.
Err 8
• “Lid Close” switch are broken on the Lid Board.
Con.
• The communication between the display & main PCB and the Display PCB fails.

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SECTION 10 – ELECTRICAL CIRCUIT DIAGRAMS

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25
SECTION 11 – WARNING LABEL

EARTHED FUSES

NF 1200R NF 1200
FUSES ( 2x10A) FUSES ( 2x4A)

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