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R-DNA Activity

This document discusses recombinant DNA technology and its applications. It begins by defining transgenic organisms and recombinant DNA, and describes restriction enzymes which cut DNA at specific sequences. The document then presents a table to illustrate how restriction enzymes cut different DNA sequences. Finally, the document poses two essay questions about the positive and negative effects of genetic engineering in medicine, and the use of transgenic pigs for organ transplants and the risks of transmitting diseases.

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Jamille Nympha C. Balasi
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155 views3 pages

R-DNA Activity

This document discusses recombinant DNA technology and its applications. It begins by defining transgenic organisms and recombinant DNA, and describes restriction enzymes which cut DNA at specific sequences. The document then presents a table to illustrate how restriction enzymes cut different DNA sequences. Finally, the document poses two essay questions about the positive and negative effects of genetic engineering in medicine, and the use of transgenic pigs for organ transplants and the risks of transmitting diseases.

Uploaded by

Jamille Nympha C. Balasi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Recombinant DNA Technology

NAME : Balasi, Jewel Emerald C. DATE

I. PART 1: Identification

1. Two names for organisms that contain foreign DNA are Transgenic Organism and Recombinant
DNA

2. The “special scissors” used to cut DNA in certain spots are called restriction enzymes.

3. Using options 1-4 at the bottom of the page, complete the table below:

Cutting DNA Sequence this Enzyme will


Pattern of Cut
Enzyme (choose from 1-4 below)

➀ ➂

➁ ➃

4. On DNA segments 1-4 above, draw lines showing where each of the restriction enzymes cleaves
them.

5. In order to form recombinant DNA, scientists have found a way to cut a DNA segment using and
enzyme called EcoRI. This enzyme cuts DNA wherever the sequence C-T-T-A-A-G occurs between
the A and the G base. Which of these would result if EcoRI were used on the DNA in the diagram
below? Circle your answer.

II. PART 2: Essay

1) Genetic engineering has good and bad effects in our society. Give an example of a positive and
negative effects of genetic engineering in the field of medicine.

2) One important factor to be considered in organ transplants is the compatibility of the donor and
the recipient. But now, doctors have learned to develop a strain of transgenic pigs that can
provide donor hearts to humans. How can cloning be helpful in providing donor hearts for
human recipients? If you are on the situation are you in favor of this? Why or why not?

Once pig organs can be transplanted into humans, transplantation will move into a new era. There will
be unlimited access to undamaged organs and cells for transplantation and, eventually, donation from
deceased or live human beings will become obsolete. Furthermore, it will be possible to alleviate graft
rejection, at least in part, by genetic modification of the source animal. If I were in this situation I am
not in favor with it because there is a risk of transmitting infectious agents from animal to man. With
regard to most microbiological agents, the risk can be minimized by using animals from strictly
controlled herds. However, such measures will not affect the porcine endogenous retrovirus (PERV).
These viruses are a permanent part of the genome in all mammalian species[12] and so all recipients of
porcine transplant will inevitably be exposed to PERV.

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