Proper collection of urine specimen
is important to maximize the
outcome of laboratory test for the
diagnosis of infectious diseases.
A variety of laboratory tests can be
performed to make a presumptive or definitive
diagnosis so that therapy can begin.
1.Clean-voided
specimen
-usually done on
the first voided
specimen in the
morning
-10ml of urine is
sufficient for
analysis
In collecting urine, the following
specific directions are required:
1.Must be free
of fecal contamination
2.Toilet tissue must not
be with the urine specimen
4.Clean the to prevent difficulty
outside of the in analyzing the result &
container with contamination
disinfectant if 3.Put the lid tightly on the
has been container to prevent
contaminated spillage
2.Clean-catch
midstream urine
-this is collected when
urine culture is
ordered to identify
microorganisms
causing UTI
-urine are collected into
a sterile specimen
with a lid.
-Clean-catch midstream urine specimens
that have more than 100,000 colonies
of bacteria per ml of urine may be
indicative of infection.
3.Timed-urine specimen (24hr urine)
-requires collection of urine over a specific
period of time ranging from 2-24 hours.
-generally refrigerated or contains a preser-
vative to decrease bacterial growth or decom-
position of urine
-each voiding of urine is collected in a small
container & emptied immediately into the
large refrigerated bottle
Purposes of timed-urine collection
1.Assess ability of the kidney to concentrate
& dilute urine
2.Determine disorders of glucose metabolism,
such as DM
3.Determine levels of specific constituents
such as albumin, amylase, creatinine in the
In a timed urine or 24 hr urine collection
Make sure to label the container with
Identifying information of the client
At the start of the collection
period have the client void
& discard this urine
Then save all urine pro-
duced during the specified
time period in the refrigerated
container & send to lab for
analysis after 24 hours
Urine consists of water and dissolved
chemicals such as sodium, potassium, urea
(formed from protein breakdown),
and creatinine (formed
from muscle breakdown)
along with other chemical
compounds
Normally, urine contains
specific amounts
of these waste products
If these amounts are not within a normal range,
or if other substances are present,
it may be an indication of a particular disease or
condition.
The results of a
24-hour urine collection
may provide information to
help your doctor make or
confirm a diagnosis.
24hour urine container
4.Indwelling catheter specimen
-sterile urine specimen can be obtained from
closed drainage system by inserting a sterile
needle with syringe
through a drainage port in the
catheter tubing.
Aspiration of urine from
catheters can be done only
with self-sealing rubber
catheters, NOT plastic or
silicone.
How to get urine sample from an indwelling
catheter?
1.Don gloves
2.Clamp the drainage
tubing 3 inches below
the sampling port for 30
minutes if there is no
urine in the catheter to
allow urine to collect in
the catheter
3. Wipe with disinfectant the area where needle
will be inserted
4.Insert the needle at 30-45 degree angle but
for the needleless port insert at 90 degree angle
5.Unclamp the catheter
6.Withdraw required amount
Of urine:
3ml for culture
30ml for routine analysis
7.Transfer urine to specified
Container
8.Discard the syringe &
needle
Foley catheter/indwelling catheter
Tip of a foley/indwelling catheter
Tips of a straight catheter
9. Cover/cap the container
10. Remove gloves & discard appropriately
11.Label the container & send to laboratory
immediately for analysis or refrigeration
12. Document any pertinent
observation of the urine
Urine Culture
A urine sample can be taken in any of the
above mentioned ways in collecting the
urine placed in a sterile bottle/container
Then send the specimen to the
Laboratory for analysis
Urine specimen must be transported
to the laboratory
promptly. If not cultured within 30 minutes
of collection, it must be refrigerated
and cultured within 24 hours.
Pediatric Urine Collector
